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Semilicoisoflavone B induces oral cancer cell apoptosis by targeting claspin and ATR-Chk1 signaling pathways

Environmental Toxicology 2024年第4期39卷 2417-2428页

作者： Hsieh, Ming-Ju Lin, Chia-Chieh Lo, Yu-Sheng Chuang, Yi-Ching Ho, Hsin-Yu Chen, Mu-Kuan Oral Cancer Research Center Changhua Christian Hospital Changhua Taiwan Doctoral Program in Tissue Engineering and Regenerative Medicine College of Medicine National Chung Hsing University Taichung Taiwan Graduate Institute of Biomedical Sciences China Medical University Taichung Taiwan Department of Otorhinolaryngology Head and Neck Surgery Changhua Christian Hospital Changhua Taiwan Department of Post-Baccalaureate Medicine College of Medicine National Chung Hsing University Taichung Taiwan

The prevalence of oral squamous cell carcinoma (OSCC) is increasing worldwide mainly due to poor oral hygiene and unrestricted lifestyle. Advanced-stage OSCC is associated with poor prognosis and a 5-year survival rate of only 30%–50%. The present study was designed to investigate the anticancer effect and mode of action of Glycyrrhiza-derived semilicoisoflavone B (SFB) in 5-fluorourasil (5FU)-resistant human OSCC cell lines. The study findings revealed that SFB significantly reduces OSCC cell viability and colony formation ability by arresting cell cycle at the G2/M and S phases and reducing the expressions of key cell cycle regulators including cyclin A, cyclin B, CDC2, and CDK2. The compound caused a significant induction in the percentage of nuclear condensation and apoptotic cells in OSCC. Regarding pro-apoptotic mode of action, SFB was found to increase Fas-associated death domain and death receptor 5 expressions and reduce decoy receptor 2 expression, indicating involvement of extrinsic pathway. Moreover, SFB was found to increase pro-apoptotic Bim expression and reduce anti-apoptotic Bcl-2 and Bcl-xL expressions, indicating involvement of intrinsic pathway. Moreover, SFB-mediated induction in cleaved caspases 3, 8, and 9 and cleaved poly(ADP-ribose) polymerase confirmed the induction of caspase-mediated apoptotic pathways. Regarding upstream signaling pathway, SFB was found to reduce extracellular signal regulated kinase 1/2 (ERK) phosphorylation to execute its pro-apoptotic activity. The Human Apoptotic Array findings revealed that SFB suppresses claspin expression, which in turn caused reduced phosphorylation of ATR, checkpoint kinase 1 (Chk1), Wee1, and CDC25C, indicating disruption of ATR-Chk1 signaling pathway by SFB. Taken together, these findings indicate that SFB acts as a potent anticancer compound against 5FU-resistant OSCC by modulating mitogen-activated protein kinase (MAPK) and ATR-Chk1 signaling pathways. © 2024 Wiley Periodicals LLC.

关键词： Phosphorylation

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Molecular dynamics simulation and purification of chimeric L1/L2 protein from human papillomavirus type 52 expressed in Escherichia coli BL21 (DE3)

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Journal of Immunoassay and Immunochemistry 2024年第5期45卷 395-414页

作者： Al Adawiah, Rabiyah Zaenal Mustopa, Apon Budiarti, Sri Nur Umami, Rifqiyah Hertati, Ai Irawan, Herman Ikramullah, Muh. Chaeril Arwansyah, Arwansyah Mamangkey, Jendri Kartikasari, Isti Salahudin Darusman, Huda Post Graduate Program of Microbiology IPB University Bogor Indonesia Research Center for Genetic Engineering National Research and Innovation Agency (BRIN) KST Soekarno Bogor Cibinong Indonesia Department of Biology IPB University Bogor Indonesia Biotechnology Study Program Postgraduate School of Universitas Gadjah Mada Yogyakarta Indonesia Department of Chemistry Education Tadulako University Palu Indonesia Department of Biology Education Faculty of Education and Teacher Training Universitas Kristen Indonesia Jakarta Indonesia Primate Animal Study Center IPB University Bogor Indonesia Department of Anatomy Physiology and Pharmacology School of Veterinary Medicine and Biomedical IPB University Bogor Indonesia Primate Animal Study Center Research Institution and Community Service (LPPM) IPB University Bogor Indonesia

The available prophylactic vaccines for human papillomavirus (HPV) in the market are only effective against specific types of HPV, rendering them ineffective for other types of HPV infections. The objective of this research is to investigate the stability of the recombinant protein constructed, namely chimeric L1/L2 protein from HPV type 52, with improved cross-neutralization ability. The 3D model, predicted using Alphafold, Robetta, I-Tasser, and refined with Galaxy Refinement, is validated using Ramachandran plot analysis. The stability is verified through molecular dynamics simulations, considering parameters such as RMSD, RMSF, Rg, and SASA, where stable conditions are observed. The chimeric L1/L2 protein from HPV type 52 is purified using affinity chromatography, and the His-tag is cleaved using SUMO protease to obtain pure chimeric protein with the size of ~ 55 kDa. Western blot analysis confirms binding to anti-L1 HPV type 52 polyclonal antibody. The obtained vaccine candidate can be utilized as an effective prophylactic vaccine against HPV. © 2024 Taylor & Francis.

关键词： HPV molecular dynamics purification vaccine

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Gas vesicle isolated from microorganisms to act as ultrasound contrast agent

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Research on biomedical engineering 2021年第1期37卷 1-7页

作者： Garrute, Felipe Vianna Machado, João Carlos Biomedical Engineering Program-COPPE Federal University of Rio de Janeiro Horácio Macedo Ave. 2030 Center of Technology Block H Room 327 Rio de JaneiroRJ21941-914 Brazil Post-graduate Program in Surgical Sciences Department of Surgery School of Medicine Federal University of Rio de Janeiro Carlos Chagas Filho Ave. 373 Health Sciences Center Block K Room 49 Rio de JaneiroRJ21941-902 Brazil

Purpose: Ultrasound contrast agent (UCA), used to enhance the contrast in ultrasonography, consists of microbubbles filled with an inert gas and encapsulated with a protein or a lipidic-stabilize membrane. The UCAs are able to resonate when exposed to ultrasound waves and to generate an echo-acoustic signal with increased intensity. Recently, a technique to produce UCA from microorganisms was developed and it consists of lysing microorganism cells to obtain gas vesicle (GV) after cultivation in the laboratory. GVs occur almost exclusively in aquatic microorganisms, as Microcystis aeruginosa (MYC), and provide buoyancy which permit to float towards the water surface. This work implemented a technique to produce GVs from MYC, at the Ultrasound Laboratory, and inspected their properties of acting as UCAs, analyzed through ultrasonic images acquired at 40 MHz, and their structural characteristics such as morphology, diameter, and length. Methods: After the cultivation of MYC, the buoyant cells with GVs were separated from the culture medium and submitted to cell lysis with chemical treatment. Thereafter, the solution with lysed cells was centrifuged, at 300g during 8 h at 4–8 °C, to isolate the GVs (absorbance 0.6, λ = 500 nm). Results: Transmission electron microscopy inspection revealed the MYC-GVs with elliptical morphology and having a diameter between 100 and 150 nm and length near to 200 nm. B-mode ultrasound images of the solutions proved the GV’s ability to generate an echo signal, thus confirming their performance as UCAs. © 2020, Sociedade Brasileira de Engenharia Biomedica.

关键词： Cytology

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Jing-Si Herbal Tea Suppresses H2O2-Instigated Inflammation and Apoptosis by Inhibiting Bax and Mitochondrial Cytochrome C Release in HIG-82 Synoviocytes

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Environmental Toxicology 2024年第12期39卷 5347-5356页

作者： Kao, Shih-Wen Chang, Yu-Chun Lin, Feng-Huei Huang, Tai-Lung Chen, Tung-Sheng Lin, Shinn-Zong Lin, Kuan-Ho Kuo, Wei-Wen Ho, Tsung-Jung Huang, Chih-Yang Graduate Institute of Aging Medicine China Medical University Taichung Taiwan Department of Orthopedic Surgery Chung-Shan Medical University Hospital Taichung Taiwan Department of Biological Science and Technology College of Life Sciences China Medical University Taichung Taiwan Cardiovascular and Mitochondrial Related Disease Research Center Hualien Tzu Chi Hospital Buddhist Tzu Chi Medical Foundation Hualien Taiwan Institute of Biomedical Engineering College of Medicine and College of Engineering National Taiwan University Taipei Taiwan Division of Biomedical Engineering and Nanomedicine Research National Health Research Institute Miaoli Taiwan Department of Orthopedics Chung-Kang Branch Cheng Ching General Hospital Taichung Taiwan School of Life Science National Taiwan Normal University Taipei Taiwan Department of Neurosurgery Hualien Tzu Chi Hospital Buddhist Tzu Chi Medical Foundation Hualien Taiwan Bioinnovation Center Buddhist Tzu Chi Medical Foundation Hualien Taiwan Department of Emergency Medicine China Medical University Hospital Taichung Taiwan College of Medicine China Medical University Taichung Taiwan Ph.D. Program for Biotechnology Industry China Medical University Taichung Taiwan School of Pharmacy China Medical University Taichung Taiwan School of Post-Baccalaureate Chinese Medicine College of Medicine Tzu chi University Hualien Taiwan Department of Chinese Medicine Hualien Tzu Chi Hospital Buddhist Tzu Chi Medical Foundation Tzu Chi University Hualien Taiwan Integration Center of Traditional Chinese and Modern Medicine Hualien Tzu Chi Hospital Buddhist Tzu Chi Medical Foundation Hualien Taiwan Graduate Institute of Biomedical Sciences China Medical University Taichung Taiwan Department of Medical Laboratory Science and Biotechnology Asia University Taichung Taiwan

Inflammation is an intrinsic protective mechanism against various forms of cellular injuries in humans;however, its undesired activation results in tissue damage and cell death. The onset of chronic inflammation and oxidative stress are the key characteristics of autoimmune inflammatory diseases such as rheumatoid arthritis (RA), for which an effective treatment is yet to be developed. Therefore, in this study, we investigated the protective effects and molecular mechanisms of a novel herbal preparation, Jing-Si herbal tea (JS), against H2O2-induced inflammation and cellular damage in HIG-82 synoviocytes. We found that JS did not show any significant alterations in cell viability at 2O2-induced pathological oxidative stress (ROS) in HIG-82 cells. Interestingly, JS treatment not only reduced the ROS levels but also significantly repressed the protein expressions of collagen degrading proteases in a dose-dependent manner. Treatment with JS showed enhanced cell viability against H2O2-induced toxic ROS levels. The expressions of cell protective aggrecan, Collagen II, and Bcl-2 were increased, whereas MMP-13, ADAMTS-2, Cytochrome C, and cleaved Caspase 3 were decreased by JS under inflammatory agents H2O2, MIA, LPS, and TNF-α treatment, respectively, in HIG-82 cells. Interestingly, the cytoprotective effect of JS treatment was attributed to a decreased mitochondrial localization of Bax and a reduction of Cytochrome C release into the cytoplasm of H2O2-treated HIG-82 cells. Collectively, our results suggest a novel protective mechanism of JS for RA treatment, which could be potentially applied as a complementary treatment or as an alternative therapeutic approach to mitigate inflammatory diseases. © 2024 Wiley Periodicals LLC.

关键词： Cell death

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Pulmonary Disease Pattern Recognition on X-Ray Radiography Image Using Artificial Neural Network (ANN) Method 3

Pulmonary Disease Pattern Recognition on X-Ray Radiography I...

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3rd Annual Scientific Meeting on Medical Physics and Biophysics, PIT-FMB in conjunction with the 17th South-East Asia Congress of Medical Physics, SEACOMP 2019

作者： Fitriyah, N. Kurniawati, L.D. Purwanti, E. Astuti, S.D. Department of Physics Faculty of Science and Technology Universitas Airlangga Surabaya60115 Indonesia Biomedical Engineering Study Program Faculty of Science and Technology Universitas Airlangga Surabaya60115 Indonesia Department of Biomedical Engineering Post-Graduate Program Faculty of Science and Technology Universitas Airlangga Surabaya60115 Indonesia

This research aims to recognize the pattern of pulmonary disease on x-ray radiography image using artificial neural network (ANN) method. The images, which were used such as images of healthy pulmonary, pulmonary tuberculosis, and pulmonary tumour. Pattern recognition was using an extraction feature of GLCM (Gray Level Co-occurrence Matrix) and back propagation method. Before being identified, the images were processed by median filter and adaptive histogram equalization. The GLCM features that used were homogeneity, energy, contrast, variance and correlation. The parameters were learning rate and hidden layer. Learning rate was 0.3 and hidden layer was 25. Back propagation training showed 100% accuracy, which all of 44 images were used had been successfully identified. From the result, the healthy pulmonary showed 60% accuracy, 83.3% for pulmonary tuberculosis and 100% for pulmonary tumor. Hence, the overall result showed 81.25% accuracy, which 13 of 16 images had been successfully identified. From these result, extraction feature of GLCM using back propagation method was capable to recognize the pattern of pulmonary disease. However, due to narrow range of the feature, this application may not be used optimally for comparing features in every category of images. Therefore, the further research is needed to determine the best features and parameters of training back propagation. © Published under licence by IOP Publishing Ltd.

关键词： Pattern recognition

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Hellebrigenin induces oral cancer cell apoptosis by modulating MAPK signalling and XIAP expression

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Journal of Cellular and Molecular Medicine 2024年第2期28卷 e18071页

作者： Hsieh, Ming-Ju Lin, Chia-Chieh Lo, Yu-Sheng Ho, Hsin-Yu Chuang, Yi-Ching Chen, Mu-Kuan Oral Cancer Research Center Changhua Christian Hospital Changhua Taiwan Doctoral Program in Tissue Engineering and Regenerative Medicine College of Medicine National Chung Hsing University Taichung Taiwan Graduate Institute of Biomedical Sciences China Medical University Taichung Taiwan Department of Post-Baccalaureate Medicine College of Medicine National Chung Hsing University Taichung Taiwan Department of Otorhinolaryngology Head and Neck Surgery Changhua Christian Hospital Changhua Taiwan

Oral squamous cell carcinoma (OSCC), which accounts for 90% of all oral cancers, has become a public health crisis worldwide. despite advances in therapeutic interventions, the prognosis remains poor for advanced-stage OSCC. In this study, we investigate the anticancer activity and the mode of action of hellebrigenin in human OSCC. The findings demonstrated that hellebrigenin exerted cytotoxic effects in OSCC cells through cell cycle arrest at the G2/M phase and downregulation of cell cycle-related proteins (cyclins A2, B1 and D3, Cdc2, CDK4 and CDK6). Moreover, hellebrigenin caused activation of PARP and caspase 3, 8 and 9, followed by downregulation of antiapoptotic proteins (Bcl-2 and Bcl-xL) and upregulation of pro-apoptotic proteins (Bax and Bak). The hellebrigenin treatment also increased Fas, DR5, DcR2 and DcR3 expressions in oral cancer cells, indicating the compound causes oral cancer cell apoptosis through both intrinsic and extrinsic pathways. Regarding upstream signalling, hellebrigenin was found to reduce the phosphorylation of ERK, p38, and JNK, indicating that hellebrigenin triggers caspase-mediated apoptosis by downregulating MAPK signalling pathway. Finally, the human apoptosis array findings revealed that hellebrigenin specifically suppressed the expression of XIAP to execute its pro-apoptotic activities. Taken together, the study suggests that hellebrigenin can act as a potent anticancer compound in human OSCC. © 2023 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley & Sons Ltd.

关键词： apoptosis hellebrigenin oral squamous cell carcinoma XIAP

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Correction to: Effect of photobiomodulation on the behaviour of mesenchymal stem cells in three‑dimensional cultures

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Lasers in medical science 2023年第1期38卷 269页

作者： Ana Laura Martins de Andrade Lucília Pereira da Silva Nivaldo Antonio Parizotto Patrícia Brassolatti Richard Eloin Liebano Alexandra Pinto Marques Physiotherapeutic Resources Laboratory Post‑Graduate Program in Physiotherapy Department of Physiotherapy Federal of University Sao Carlos Sao Carlos Sao Paulo Brazil. Post‑Graduate Program in Biomedical Engineering Department of Biomedical Engineering University Brazil Campus Itaquera Rua Carolina Fonseca 584 Vila Santana Sao Paulo SP 08230030 Brazil. 3B's Research Group Biomaterials Biodegradable and Biomimetics Avepark Parque de Ciencia E Tecnologia Zona Industrial da Gandra 4805‑017 Barco Guimaraes Portugal. ICVS/3B's - PT Government Associate Laboratory 4805‑017 Braga Guimaraes Portugal. Physiotherapeutic Resources Laboratory Post‑Graduate Program in Physiotherapy Department of Physiotherapy Federal of University Sao Carlos Sao Carlos Sao Paulo Brazil. nivaldoaparizotto@***. Post‑Graduate Program in Biomedical Engineering Department of Biomedical Engineering University Brazil Campus Itaquera Rua Carolina Fonseca 584 Vila Santana Sao Paulo SP 08230030 Brazil. nivaldoaparizotto@***. Department of Morphology and Pathology Post‑Graduate Program in Evolutionary Genetics and Molecular Biology Federal University of Sao Carlos (UFSCar) Sao Carlos Sao Paulo Brazil.

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Bilayer osteochondral graft in rabbit xenogeneic transplantation model comprising sintered 3D-printed bioceramic and human adipose-derived stem cells laden biohydrogel

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Journal of Biological engineering 2023年第1期17卷 1-24页

作者： Lee, Chih-Yun Nedunchezian, Swathi Lin, Sung-Yen Su, Yu-Feng Wu, Che-Wei Wu, Shun-Cheng Chen, Chung-Hwan Wang, Chih-Kuang Ph.D. Program in Life Sciences College of Life Science Kaohsiung Medical University Kaohsiung 80708 Taiwan Regenerative Medicine and Cell Therapy Research Center Kaohsiung Medical University Kaohsiung 80708 Taiwan Orthopaedic Research Center College of Medicine Kaohsiung Medical University Kaohsiung 80708 Taiwan Department of Medicinal and Applied Chemistry College of Life Science Kaohsiung Medical University Kaohsiung 80708 Taiwan Departments of Orthopaedics School of Medicine College of Medicine Kaohsiung Medical University Kaohsiung 80708 Taiwan Department of Orthopaedics Kaohsiung Medical University Hospital Kaohsiung Medical University Kaohsiung 80708 Taiwan Department of Orthopaedics Kaohsiung Municipal Ta-Tung Hospital Kaohsiung Medical University Kaohsiung 80145 Taiwan Faculty of Post-Baccalaureate Medicine College of Medicine Kaohsiung Medical University Kaohsiung 80756 Taiwan Department of Surgery Division of Neurosurgery Kaohsiung Medical University Hospital Kaohsiung Medical University Kaohsiung 80708 Taiwan Department of Nursing Asia University Taichung 41354 Taiwan Ph.D. Program in Biomedical Engineering College of Medicine Kaohsiung Medical University Kaohsiung 80708 Taiwan Graduate Institute of Medicine College of Medicine Kaohsiung Medical University Kaohsiung 80708 Taiwan

Reconstruction of severe osteochondral defects in articular cartilage and subchondral trabecular bone remains a challenging problem. The well-integrated bilayer osteochondral graft design expects to be guided the chondrogenic and osteogenic differentiation for stem cells and provides a promising solution for osteochondral tissue repair in this study. The subchondral bone scaffold approach is based on the developed finer and denser 3D β-tricalcium phosphate (β-TCP) bioceramic scaffold process, which is made using a digital light processing (DLP) technology and the novel photocurable negative thermo-responsive (NTR) bioceramic slurry. Then, the concave-top disc sintered 3D-printed bioceramic incorporates the human adipose-derived stem cells (hADSCs) laden photo-cured hybrid biohydrogel (HG + 0.5AFnSi) comprised of hyaluronic acid methacryloyl (HAMA), gelatin methacryloyl (GelMA), and 0.5% (w/v) acrylate-functionalized nano-silica (AFnSi) crosslinker. The 3D β-TCP bioceramic compartment is used to provide essential mechanical support for cartilage regeneration in the long term and slow biodegradation. However, the apparent density and compressive strength of the 3D β-TCP bioceramics can be obtained for ~ 94.8% theoretical density and 11.38 ± 1.72 MPa, respectively. In addition, the in vivo results demonstrated that the hADSC + HG + 0.5AFnSi/3D β-TCP of the bilayer osteochondral graft showed a much better osteochondral defect repair outcome in a rabbit model. The other word, the subchondral bone scaffold of 3D β-TCP bioceramic could accelerate the bone formation and integration with the adjacent host cancellous tissue at 12 weeks after surgery. And then, a thicker cartilage layer with a smooth surface and uniformly aligned chondrocytes were observed by providing enough steady mechanical support of the 3D β-TCP bioceramic scaffold. © 2023, The Author(s).

关键词： Bioceramic scaffold Digital light processing Gelatin methacryloyl Hyaluronic acid methacryloyl Osteochondral Tissue engineering

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Fuzzy sports motivational system for measuring psychological and training issues in running recreational practice and adherence

Fuzzy sports motivational system for measuring psychological...

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Decision Aid Sciences and Application (DASA), International Conference on

作者： Vanessa C. M. Ribeiro Ernesto Araujo Biomedical Engineering Post-Graduate Program Universidade Anhembi Morumbi (UAM) SP Brazil Universidade Anhembi-Morumbi (UAM) São Paulo SP Brazil Centro de Inovação Tecnologia e Educação (CITE) SP Brazil Inteligência Artificial em Tecnologia (IATECH) SP Brazil

ISBN: (纸本)9781665416351

A fuzzy system for assessing sports adherence and practice by taking into account motivational training and psy-chological factors for recreational runners is proposed in this paper. Running is a sport that has continuously been increasing and recreational runners, mostly characterized by amateur ath-letes, are characterized as presenting diverse and multifaceted profile. Such running sports practitioners present interests from promotion of health and attending races up to achieve social interaction and obtain pleasant moments. In consonance with this perspective, the proposed fuzzy IF-THEN system is designed by employing psychological and practice aspects to assess the level of interest, motivation, and adherence of recreational runners. In this sense, the fuzzy inference system uses as linguistic input variables the psychological level of satisfaction concerning the training in group (collective training) and coach's professional expertise (professional quality of the coach), two prominent motivation factors among recreational sports practitioners. The fuzzy set theory and fuzzy logic is used to represent the subtle and subjective reasoning concerning the sport perception. Such a fuzzy motivational assessment enables to stratify and score, by qualitatively and quantitatively measure, the impact in the adherence in the sports practice of recreational runners.

关键词： Training Fuzzy logic Input variables Current measurement Psychology Linguistics Cognition

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Noncontact monitoring of the peripheral microcirculation using laser speckle contrast imaging to evaluate the skin perfusion pressure

Results in Engineering

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Results in engineering 2025年 26卷

作者： Meng-Che Hsieh Rou-Zheng Lai Ching-Han Hsu Yan-Ren Lin Pei-You Hsieh Congo Tak Shing Ching Lun-De Liao Institute of Biomedical Engineering and Nanomedicine National Health Research Institutes 35 Keyan Road Zhunan Town Miaoli County 350 Taiwan Doctoral Program in Tissue Engineering and Regenerative Medicine National Chung Hsing University 145 Xingda Road South District Taichung City 402 Taiwan Department of Biomedical Engineering and Environmental Sciences National Tsing Hua University No. 101 Section 2 Kuang-Fu Road Hsinchu 30013 Taiwan Department of Emergency and Critical Care Medicine Changhua Christian Hospital Changhua Taiwan Department of Post-Baccalaureate Medicine College of Medicine National Chung Hsing University 145 Xingda Road South District Taichung City 402 Taiwan School of Medicine Chung Shan Medical University 110 Section 1 Jianguo North Road Taichung City 402 Taiwan Graduate Institute of Biomedical Engineering National Chung Hsing University 145 Xingda Road South District Taichung City 402 Taiwan Department of Electrical Engineering National Chi Nan University Puli Township 54561 Taiwan International Doctoral Program in Agriculture National Chung Hsing University Taichung 402 Taiwan

The skin perfusion pressure (SPP) is traditionally measured with laser Doppler flowmetry (LDF), but this technique is limited by its single-point fiber optic probe, the need to contact the skin, and susceptibility to various factors, resulting in poor data consistency. Therefore, we developed a noncontact system for measuring the SPP over a large area based on laser speckle contrast imaging (LSCI). When it was paired with a pressure cuff device, the system accurately recorded blood flow changes during various pressure interventions, and the reperfusion pressure point could be calibrated to overcome traditional measurement limitations. Cross-comparison experiments revealed that the measurements obtained with the LSCI and LDF systems were highly correlated (r > 0.7), confirming the reliability of LSCI in assessing microcirculatory function. Since supine measurements are not always suitable in clinical environments, such as in space-constrained clinics or when the patient cannot lie flat, we compared SPP measurements obtained from individuals in sitting versus supine positions, revealing minimal differences (Cohen's d = 0.1, p > 0.05), which demonstrates the system's stability over different patient postures. Under cold water stimulation, which induces microvascular contraction, simulating poor circulation, the measured SPP was significantly lower than that measured under normal conditions (Cohen's d = 2.6, p < 0.05), verifying the system's potential applicability under different pathological conditions. According to our results, the LSCI system not only obtains SPP measurements that are correlated with the LDF-measured SPP but also overcomes the limitations of the latter system, providing noncontact, instant, and large-scale microcirculation monitoring. This study offers a reliable microcirculatory assessment tool based on LSCI technology, and the results support the future application of this tool in the early diagnosis and monitoring of diabetic foot neuropathy and

关键词： Laser speckle contrast imaging Skin perfusion pressure Laser doppler flowmetry Microcirculation Noninvasive measurement

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