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Response to Commentary by Dr. Matthew J. Clarkson on

Journal of Sport and Health Science 2024年第5期13卷 670-671页

作者： Hugo de Luca Corrêa Lysleine Alves Deus Dahan da Cunha Nascimento Nicholas Rolnick Rodrigo Vanerson Passos Neves Andrea Lucena Reis Thais Branquinho de Araújo Carmen Tzanno-Martins Fernanda Silveira Tavares Luiz Sinésio Silva Neto Cláudio Avelino Rodrigues Santos Paolo Lucas Rodrigues-Silva Fernando Honorato Souza Vitória Marra da Motta Vilalva Mestrinho Rafael Lavarini dos Santos Rosangela Vieira Andrade Jonato Prestes Thiago dos Santos Rosa Post-graduate Program of Physical Education Catholic University of BrasıliaBrasılia 71966-700Brazil The Human Performance Mechanic Lehman CollegeNew YorkNY 10011USA Clinical Group Home Dialysis Center and RenalClass Sao Paulo 01239-040Brazil Department of Medicine Catholic University of BrasıliaBrasılia 71966-700Brazil Faculty of Medicine Federal University of TocantinsTocantins 77001-090Brazil Embrapa Genetic Resources and Biotechnology Brasılia 70770-917Brazil g Graduate Program of Genomic Sciences and Biotechnology Brasılia 71966-700Brazil

Dear editor:We sincerely thank Matthew *** for his insightful commentary1 on our recent publication in the Journal of Sport and Health Science.2 Clarkson's pioneering work in the development and application of blood flow restriction (BFR) exercise protocols has undeniably laid the groundwork for subsequent investigations in this field,including our own.(3-6)His commentary not only highlights the relevance and timeliness of our study but also offers valuable perspectives on refining safety considerations for intradialytic BFR exercise among hemodialysis patients.

关键词： patients application flow

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Multipurpose Silicon-Based Toothpastes: Decision Making for a Better Oral Health

European Dental Research and Biomaterials Journal

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European Dental Research and Biomaterials Journal 2023年第1/02期03卷 001-002页

作者： Fabiano Vieira Vilhena Paulo Henrique Perlatti D'Alpino Trials Research and Development Inc. São Paulo SP Brazil Triplet Biotechnology Solutions São Paulo SP Brazil São Paulo State University (UNESP) School of Sciences POSMAT – Post-Graduate Program in Materials Science and Technology Bauru SP Brazil

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Fatty acids, physical-chemical properties, minerals, total phenols and anti-acetylcholinesterase of abiu seed oil

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Chemical Engineering Transactions 2018年 64卷 283-288页

作者： de Melo Filho, Antonio A. da Costa, Ana Márcia D. C. Fernández, Ismael M. dos Santos, Ricardo C. Chagas, Edvan A. Chagas, Pollyana C. Takahashi, Jacqueline A. Ferraz, Vany P. Post Graduate Program in Chemistry PPGQ and Chemistry Department UFRR Campus Paricarana Boa Vista-RR Brazil Post Graduate Program in Biodiversity and Biotechnology Bionorte UFRR Campus Paricarana Boa VistaRR Brazil National Postdoctoral Program of CAPES PNPD/CAPES associated to the POSAGRO/UFRR Boa Vista-RR Brazil Embrapa Rodovia 174 Km 8 Industrial District Boa Vista-RRCEP 69301970 Brazil Post Graduate in Agronomy POSAGRO UFRR Campus Cauamé BR 174 District Monte Cristo Boa Vista-RR Brazil Department of Chemistry Institute of Exact Sciences Federal University of Minas Gerais UFMG Belo Horizonte-MG Brazil Chromatography Laboratory Institute of Exact Sciences UFMG CEP 31270-901 Belo Horizonte-MG Brazil

ISBN: (纸本)9788895608563

The aim of this work is to perform analysis of the oil of the seed of abiu (Pouteria caimito) the presence of minerals (ICP-OES), fatty acids (GC-FID), physical-chemical properties (1H NMR) and acetylcholinesterase activity (AChE). Thus, the following results were obtained for the majority fatty acids saturated: palmitic acid (27.3%), and unsaturated: oleic acid (43.1%), linoleic acid (8.6%), a-linolenic acid (0.4%), and ?-linolenic acid (0.4%). The physical-chemical properties of this oil were: iodine index (42.72 mg I2 g-1), saponification index (230.87 mg KOH g-1), acid number (1.50 mg KOH g-1) and the average molecular weight (710.54 g mol-1). For minerals: phosphorus (8.62 mg 100g-1), calcium (3.50 mg 100g-1), sodium (3.40 mg 100g-1), potassium (2.62 mg 100g-1), magnesium (2.29 mg 100g-1), sulfur (1.54 mg 100 g-1). The oil of the abiu seed had 68.40% inhibition on AChE and was therefore considered a potent inhibitor and the oil of abiu presents 35.47 mg EAG. 100 g-1 in its composition. The abiu is a tree that belongs to the Sapoteaceae family, originating in the Amazon region near the Andean slopes of Peru and the western Brazilian Amazon, easily found in the wild. The abiu is closely tropical or subtropical, adapts to the hot and humid climate, and presents better development conditions when it is located near the equator. Copyright © 2018, AIDIC Servizi S.r.l.

关键词： Chemical analysis

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Bioactive extracts from swartzia latifolia

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Chemical Engineering Transactions 2018年 64卷 211-216页

作者： da Costa, Gilzonia Veloso da Costa, Habdel N. R. dos Santos, Ricardo C. de Melo Filho, Antônio A. Chagas, Edvan A. Montero, Ismael F. Chagas, Pollyana C. de Melo, Ana Cristina G. R. Campus Paricarana Boa Vista-RRCEP 69304-000 Brazil Post-Graduate Program in Chemistry PPGQ Center for Research and Graduate Studies in Science and Technology NPPGCT UFRR and Chemistry Department UFRR Campus Paricarana Boa Vista-RRCEP 69304-000 Brazil National Postdoctoral Program of CAPE associated to POSAGRO/UFRR Campus Cauamé BR 174 s/n Km 12 District Monte Cristo Boa Vista-RRCEP 69310-250 Brazil Embrapa Rodovia 174 Km 8 Industrial District Boa Vista-RRCEP 69301970 Brazil Post Graduate Program in Biodiversity and Biotechnology Bionorte State Coordination of Roraima UFRR Campus Paricarana Boa Vista-RRCEP 69304-000 Brazil Post Graduate in Agronomy POSAGRO UFRR Campus Paricarana Boa Vista-RRCEP 69304-000 Brazil

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Evaluation of total phenolic compounds and antioxidant activity in amazon fruit

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Chemical Engineering Transactions 2018年 64卷 649-654页

作者： Ismael Montero, F. Chagas, Edvan A. Melo Filho, Antonio A. D. E. Saravia, Selvin A. M. Santos, Ricardo C. Chagas, Pollyana Cardoso Ednalva da, D. R. S. Duarte Post Graduate Program in Biodiversity and Biotechnology Bionorte State Coordination of Roraima UFRR Campus Paricarana Boa Vista-RRCEP 69304-000 Brazil Embrapa Rodovia 174 Km 8 Industrial District Boa VistaRRCEP 69301970 Brazil Post-Graduate Program in Chemistry PPGQ Center for Research and Graduate Studies in Science and Technology NPPGCT UFRR Campus Paricarana Boa Vista-RRCEP 69304-000 Brazil Post Graduate in Agronomy POSAGRO UFRR Campus Paricarana Boa Vista-RRCEP 69304-000 Brazil National University of Agriculture Carretera a Dulce Nombre de Culmi El Espino Catacamas-Olancho Spain National Postdoctoral Program of CAPES PNPD/CAPES associated to POSAGRO/UFRR Campus Cauamé BR 174 s/n Km 12 District Monte Cristo Boa Vista-RRCEP 69310-250 Brazil Graduate Program in Chemistry Center in Science and Technology CCT UFRR Campus Paricarana Boa Vista-RRCEP 69304-000 Brazil

ISBN: (纸本)9788895608563

In this work, nine fruits cultivated in the northern Amazon were studied: abiu (Pouteria caimito), acerola (Malpighia emarginata), araçá (Psidium cattleianum), bacuparí (Rheedia gardneriana), biribá (Rollinia mucosa), caçarí (Myrciaria dubia) (Annona squamosa), graviola (Annona muricata) and taperebá (Spondias mombin L.). The total phenolic compounds were evaluated in the pulp, seed and bark by means of the colorimetric reaction of Folin Ciocateau, as well as the antioxidant capacity in the different extracts. DPPH method and on the other hand by the iron reduction method. As the fruits that presented a greater quantity of phenolic compounds are in mg gallic acid. 100g-1 sample, we have: bark of camu camu (1241.1 ± 12.04), followed by the abiu bark with (1132,43 ± 8,10), araçá pulp (1080,21 ± 1,1) and Pulp of acerola (1071.4 ± 22.2). Evaluating the antioxidant capacity, the evaluated fruits that present a higher value of antioxidant capacity are the Araçá seed with EC50 value of (471,23 ± 21,23 g / g DPPH) and for iron reduction of (57,21 ± 4.11 mmol Fe2SO4 / g), followed by the EC50 of abiu bark (521.71 ± 1.34 g / g DPPH) and iron reduction of 411.43 ± 27.12 µmol Fe2SO4 / g), and for the camu-camu pulp (549.24 ± 21.13 g / g DPPH) and for reduction of iron (235.47 ± 11.44 mmol Fe2SO4 / g). Multivariate analysis methods were applied through Principal Component Analysis (PCA) with pulp having the highest correlation between data variability with 93.6% according to PCA. Copyright © 2018, AIDIC Servizi S.r.l.

关键词： Fruits

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Biological activity of hexane extracts of the northern amazon species capsicum spp.

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Chemical Engineering Transactions 2018年 64卷 277-282页

作者： Morais, Karla S. de Melo Filho, Antonio A. Vilarinho, Lucianne B. O. Morais, Bruna S. Chagas, Pollyana C. dos Santos, Ricardo C. Reis Melo, Ana C. G. Takahashi, Jacqueline A. Department of Health Federal Institute of Roraima IFRR Boa Vista Campus CEP 69.303-340 Boa Vista-RR Brazil Post-Graduate in Biotechnology and Biodiversity Program Departament of Chemistry and Post-Graduate Program in Chemistry PPGQ Center for Research and Graduate Studies in Science and Technology NPPGCT Federal University of Roraima UFRR Paricarana Campus Boa Vista-RRCEP 69304-000 Brazil Post Graduate in Agronomy POSAGRO UFRR Campus Paricarana Boa Vista-RRCEP 69304-000 Brazil Cauamé Campus BR 174 s/n Km 12 District Monte Cristo Boa Vista-RRCEP 69.301-970 Brazil Department of Chemistry Institute of Exact Sciences Federal University of Minas Gerais UFMG CEP 31270-901 Belo HorizonteMinas Gerais Brazil

ISBN: (纸本)9788895608563

The objective of this study was to evaluate the presence of inhibitory substances from the fruits of Capsicum spp. var. Canaimé against micro-organisms indicators. Mature fruits (1.0 kg) were purchased at an agricultural fair, dried in an oven, and then was continuously extracted on a Soxhlet apparatus for 6 h, using 500 mL of hexane. Gram-positive Staphylococcus aureus (ATCC 25923), Listeria monocytogenes (ATCC 15313) and Bacillus cereus (ATCC 49456) were used to perform the antibacterial activity, as well as three Gram-negative strains: Salmonella typhimurium (ATCC 14028), Citrobacter freundi (ATCC 8090) and Pseudomonas aeruginosa (ATCC 25922). The antibacterial activity of hexane extract of Capsicum spp. fruits in some concentrations, was quite high when compared with the antibiotic. The level of inhibition was better towards Gram (-) bacteria compared to Gram (+) bacteria. The excellent bioactivity of the extract against S. typhimurium may be related to its selectivity. The present study demonstrated the efficacy of Capsicum spp. extract against pathogens that affect the health of human beings and presence of chemical assets that aid in the prevention of diseases. However, more in-depth studies are still needed to consolidate its use and application in pharmaceutical area. Copyright © 2018, AIDIC Servizi S.r.l.

关键词： Fruits

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Chemical characterization of oils and fats from amazonian fruits by1H NMR

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Chemical Engineering Transactions 2018年 64卷 235-240页

作者： Santos, Ricardo C. Chagas, Edvan A. de Melo Filho, Antonio A. Takahashi, Jacqueline A. Montero, Ismael F. dos Santos, Gabriel F. Chagas, Pollyana C. de Melo, Cristina G. R. National Postdoctoral Program of CAPES PNPD/CAPES associated to the Postgraduate Program in Agronomy of Universidade Federal de Roraima POSAGRO/UFRR Campus Cauamé BR 174 s/n Km 12 District Monte Cristo Boa Vista-RRCEP 69310-250 Brazil Brazilian Agricultural Research Corporation Embrapa Rodovia 174 Km 8 Industrial District Boa VistaRRCEP 69301970 Brazil Embrapa Boa Vista-RR-Brazil CNPq Research Produtivity Scholarship Brazil Post-Graduate Program in Chemistry PPGQ Center for Research and Graduate Studies in Science and Technology NPPGCT UFRR Campus Paricarana Boa Vista-RRCEP 69304-000 Brazil Department of Chemistry Institute of Exact Sciences Universidade Federal de Minas Gerais UFMG Av Antonio Carlos no. 6627 Pampulha Belo Horizonte-MGCEP 31270-901 Brazil Post Graduate Program in Biodiversity and Biotechnology Bionorte State Coordination of Roraima UFRR Campus Paricarana Boa Vista-RRCEP 69304-000 Brazil Post Graduate in Agronomy POSAGRO/UFRR Campus Paricarana Boa Vista-RRCEP 69304-000 Brazil Post-Graduate in Natural Resources Program PPGRN/UFRR Boa Vista-RRCEP 69304-000 Brazil

ISBN: (纸本)9788895608563

Native plants from the Brazilian Amazon Biome are rich in nutrients, oils and fats and, therefore, represent a very important regional food resource. There is a global interest in the nutritional potential of tropical fruits, the potential of Amazonian fruits as source of essential fatty acids is still under exploited, since their composition is still understudied.1H Magnetic Resonance Nuclear (1H NMR) is a robust spectroscopic tool to profile fatty acids components in oils. The objectives of this work were to use1H NMR to determine the fatty acids profile and the physicochemical properties of crude oils and fats obtained from fruits of Brazilian Amazon: açaí, ata-brava, bacaba, buriti, buritirana, yellow murici, red murici, piaçaba braba, tucumanzinho, pupunha red and yellow varieties. Oils were obtained by Soxhlet extraction using hexane as a solvent. After solvent removal by rotoevaporation, the resulting oils and fats were analyzed by1H NMR spectroscopy. Unsaturated fatty acids oleic (?9), linoleic (?6) and linolenic (?3) acids were quantified in the oils and their physicochemical properties were determined. It was observed that the oils have predominately ?9 fatty acids, followed by ?6 and ?3 representatives. The physicochemical properties confirmed edibility and good quality of oils and fats present in the aforementioned Amazonian fruits. Copyright © 2018, AIDIC Servizi S.r.l.

关键词： Fruits

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Targeting DNA junction sites by bis-intercalators induces topological changes with potent antitumor effects

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Nucleic acids research 2024年第15期52卷 9303-9316页

作者： Shih-Chun Huang Chia-Wei Chen Roshan Satange Chang-Chih Hsieh Chih-Chun Chang Shun-Ching Wang Chi-Li Peng Tai-Lin Chen Ming-Hsi Chiang Yih-Chern Horng Ming-Hon Hou Doctoral Program in Medical Biotechnology National Chung Hsing University Taichung 402 Taiwan. Graduate Institute of Genomics and Bioinformatics National Chung Hsing University Taichung 402 Taiwan. Department of Chemistry National Changhua University of Education Changhua 50058 Taiwan. Institute of Chemistry Academia Sinica Taipei 11528 Taiwan. Graduate Institute of Biotechnology National Chung Hsing University Taichung 402 Taiwan. Post Baccalaureate Medicine School of Medicine National Chung Hsing University Taichung 402 Taiwan. Biotechnology Center National Chung Hsing University Taichung 402 Taiwan.

Targeting inter-duplex junctions in catenated DNA with bidirectional bis-intercalators is a potential strategy for enhancing anticancer effects. In this study, we used d(CGTATACG)2, which forms a tetraplex base-pair junction that resembles the DNA-DNA contact structure, as a model target for two alkyl-linked diaminoacridine bis-intercalators, DA4 and DA5. Cross-linking of the junction site by the bis-intercalators induced substantial structural changes in the DNA, transforming it from a B-form helical end-to-end junction to an over-wounded side-by-side inter-duplex conformation with A-DNA characteristics and curvature. These structural perturbations facilitated the angled intercalation of DA4 and DA5 with propeller geometry into two adjacent duplexes. The addition of a single carbon to the DA5 linker caused a bend that aligned its chromophores with CpG sites, enabling continuous stacking and specific water-mediated interactions at the inter-duplex contacts. Furthermore, we have shown that the different topological changes induced by DA4 and DA5 lead to the inhibition of topoisomerase 2 activities, which may account for their antitumor effects. Thus, this study lays the foundations for bis-intercalators targeting biologically relevant DNA-DNA contact structures for anticancer drug development.

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Molecular dynamics simulation and purification of chimeric L1/L2 protein from human papillomavirus type 52 expressed in Escherichia coli BL21 (DE3)

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Journal of Immunoassay and Immunochemistry 2024年第5期45卷 395-414页

作者： Al Adawiah, Rabiyah Zaenal Mustopa, Apon Budiarti, Sri Nur Umami, Rifqiyah Hertati, Ai Irawan, Herman Ikramullah, Muh. Chaeril Arwansyah, Arwansyah Mamangkey, Jendri Kartikasari, Isti Salahudin Darusman, Huda Post Graduate Program of Microbiology IPB University Bogor Indonesia Research Center for Genetic Engineering National Research and Innovation Agency (BRIN) KST Soekarno Bogor Cibinong Indonesia Department of Biology IPB University Bogor Indonesia Biotechnology Study Program Postgraduate School of Universitas Gadjah Mada Yogyakarta Indonesia Department of Chemistry Education Tadulako University Palu Indonesia Department of Biology Education Faculty of Education and Teacher Training Universitas Kristen Indonesia Jakarta Indonesia Primate Animal Study Center IPB University Bogor Indonesia Department of Anatomy Physiology and Pharmacology School of Veterinary Medicine and Biomedical IPB University Bogor Indonesia Primate Animal Study Center Research Institution and Community Service (LPPM) IPB University Bogor Indonesia

The available prophylactic vaccines for human papillomavirus (HPV) in the market are only effective against specific types of HPV, rendering them ineffective for other types of HPV infections. The objective of this research is to investigate the stability of the recombinant protein constructed, namely chimeric L1/L2 protein from HPV type 52, with improved cross-neutralization ability. The 3D model, predicted using Alphafold, Robetta, I-Tasser, and refined with Galaxy Refinement, is validated using Ramachandran plot analysis. The stability is verified through molecular dynamics simulations, considering parameters such as RMSD, RMSF, Rg, and SASA, where stable conditions are observed. The chimeric L1/L2 protein from HPV type 52 is purified using affinity chromatography, and the His-tag is cleaved using SUMO protease to obtain pure chimeric protein with the size of ~ 55 kDa. Western blot analysis confirms binding to anti-L1 HPV type 52 polyclonal antibody. The obtained vaccine candidate can be utilized as an effective prophylactic vaccine against HPV. © 2024 Taylor & Francis.

关键词： HPV molecular dynamics purification vaccine

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Neuromotor and Functional Adaptations in Schoolchildren Practicing Exergames

Neuromotor and Functional Adaptations in Schoolchildren Prac...

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Technology Innovations for Healthcare (ICTIH), International Conference on

作者： Estelio Henrique Martin Dantas Karollyni Bastos Andrade Madson Rodrigo Silva Bezerra Renato Ramos Coelho Estevão Scudese Eric Marcos Nunes Calvacante João Wolney Teles Soares Thigna De Carvalho Batista Roberto Jerônimo Dos Santos Silva Master and Doctor's Degree Program in Health and Environment - PSA Tiradentes University - UNIT Doctor's Degree Program in Nursing and Biosciences - PPgEnfBio Federal University of the State of Rio de Janeiro UNIRIO Rio de Janeiro Brazil Aracaju Brazil Master and Doctor's Degree Program in Health and Environment PSA Tiradentes University (UNIT) Aracaju Brazil Post graduate Program in Biotechnology of RENORBIO Aracaju Brazil Nomus Consultoria Belo Horizonte Brazil Doctor's Degree Program in Nursing and Biosciences - PPgEnfBio Federal University of the State of Rio de Janeiro - UNIRIO Rio de Janeiro Brazil Unama (Universidade da Amazônia) Belém Brazil Universidade Tiradentes Aracaju Brazil Post graduate Program in Physical Education Federal University of Sergipe São Cristóvão Brazil

Exergames can become an alternative tool for enhancing neuromotor capacity from routines and systematization. Thirty-two students (age group: 8 - 10 years) were evaluated and divided into two random groups, the control group - CG (n = 10) and the exergame group - EG (n = 22). Flexibility capacity and abdominal and upper limbs resistance were evaluated before and after 16 weeks. A 2x2 factorial ANOVA, post-Hoc Bonferroni, and the effect size was calculated using SPSS for Windows v.23. Significant changes in flexibility were observed, which F (1.30) = 7.683; p < 0.01; r = 0.45; EG presented significant alteration F (1.30) = 15.1; p < 0.01; r = 0.57, however, CG showed no significant changes F (1.30) = 0.52; p = 0.47; r = 0.13. For the upper limbs strength analysis, only EG presented significant adaptation F (1.30) = 5.01; p < 0.05; r = 0.37. Finally, there were no significant changes in the variable abdominal strength.

关键词： Resistance Technological innovation Medical services Analysis of variance

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