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2023 IEEE International Ultrasonics Symposium, IUS 2023

作者： Garrute, Felipe Vianna Pacheco, Ana Beatriz F. Lu, George MacHado, João Carlos Federal University of Rio de Janeiro Biomedical Engineering Program Coope Rio de Janeiro Brazil Federal University of Rio de Janeiro Biophysics Institute Carlos Chagas Filho Rio de Janeiro Brazil Willian Rice University Bioscience Research Collaborative Houston United States Federal University of Rio de Janeiro Biomedical Engineering Program/COOPE Post-Graduation Program in Surgical Sciences Rio de Janeiro Brazil

ISBN: (纸本)9798350346459

Gas vesicles (GVs), nanostructure gas-filled protein-shelled compartments, act as ultrasound contrast agents (UCAs). Recent works report bioengineered GvpC, one of the shell proteins, potentializes interactions with specific molecules in tumor microenvironment. An important target of investigation is Cathepsin B (CTSB), an enzyme produced by several tumors. CTSB acts in the tumoral microenvironment to increase tumor growth and invasion. This work objective relied on bioengineering the GVs to promote the GvpC-CTSB interaction with a subsequent increase of ultrasound (US) signal backscattered. GvpC was engineered to contain a known CTSB cleavage sequence re-added in striped GV (ΔGV). Western Blot (WB) test was used to compare the GVwt (wild type) and GV∗ (GV engendered) before and after 24 hours reaction with CTSB. US images for PBS (negative control), GVtw, ΔGV, GV∗ and CTSB+GV∗, were obtained in 21MHz (5% total power) and regions-of-interest (ROIs) over the final B-mode image were determined. The WB test for GVwt resulted in mean (±SD) GvpC concentrations of 25169 (±8574) and 32873 (±9113) and GV∗ was 32487 (±6867) and 12763 (±2846), at t0 and t24, respectively. The US image gray level intensities for the ROIs related to the samples of PBS, GVwt, ΔGV, GV∗ and CTSB+GV∗ were 55.8 (±17.6), 47.4 (±16.6), 46.1 (±22.8) and 150.2 (±24), respectively. These results reveal an increase in the US signal backscattered from GV∗ after CTSV reaction. This increase is likely related to a direct GvpC degradation by CTSB, without affecting the GV∗ structure. In conclusion, high frequency US B-mode images showed the GV∗ with the potential to act as an UCA sensitive to the CTSB presence in tumoral microenvironment. © 2023 IEEE.

关键词： Ultrasonics

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Use of Endoluminal and Transabdominal Ultrasound Biomicroscopy to Measure Colon Wall Thickness in Healthy and Tumor-Bearing Mice

Use of Endoluminal and Transabdominal Ultrasound Biomicrosco...

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Ultrasonics Symposium (LAUS), IEEE UFFC Latin America

作者： Juliana Oliveira da Silva Fernandes Rodrigo Pereira de Oliveira João Carlos Machado Biomedical Engineering Program/COPPE Federal University of Rio de Janeiro Rio de Janeiro Brazil Biomedical Engineering Program/COPPE and Post-Graduation Program in Surgical Sciences/Department of Surgery/School of Medicine Federal University of Rio de Janeiro Rio de Janeiro Brazil

ISBN: (数字)9798350349085

ISBN: (纸本)9798350349092

Colorectal cancer (CRC) ranks among the most prevalent tumors, particularly affecting men and women, with mouse models and imaging technologies like colonoscopy, endoluminal (eUBM), and transabdominal (tUBM) ultrasound biomicroscopy playing pivotal roles in exploring new treatment avenues. The evaluation of colon wall thickness (Wt), a significant marker of tumor presence, is feasible through eUBM or tUBM, contrasting with colonoscopy's limitations. This study scrutinizes the consistency of Wt measurements obtained via eUBM and tUBM in mice, with or without colon tumors induced by azoxymethane (AOM) and dextran sulfate sodium salt (DSS). UBM images were captured using both eUBM and tUBM instruments, with subsequent Wt measurements performed using ImageJ software. Comparison between Wt obtained from eUBM and tUBM images used the One-Way ANOVA, for normal distributed data, or the Kruskal-Wallis test otherwise. Local Ethics Committee for Animal Use in Research approved the work (protocol 014/20). In general, the results indicate a substantial median Wt disparity between control and tumor groups measured by both techniques (p<0.05). Notably, discrepancies in Wt findings between the two methods were observed in the control group (p<0.05), despite the excellent resolution of the images in delineating colon layers.

关键词： Ultrasonic imaging Ultrasonic variables measurement Colonoscopy Mice Colon Software measurement Spatial resolution

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Exploring the Impact on Collapse and Ultrasound Backscattered Signal Intensity with Varying Ultrasound Power Incident on Gas Vesicle Used for Contrast Agent

Exploring the Impact on Collapse and Ultrasound Backscattere...

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Ultrasonics Symposium (LAUS), IEEE UFFC Latin America

作者： Mariana Rocha Da Silva Flávia Maria Carra Pereira De Jesus Felipe Vianna Garrute João Carlos Machado Biomedical Engineering Program-COPPE Federal University of Rio de Janeiro Rio de Janeiro Brazil Department of Surgery Biomedical Engineering Program-COPPE and Post-Graduation Program in Surgical Sciences School of Medicine Federal University of Rio de Janeiro Rio de Janeiro Brazil

ISBN: (数字)9798350349085

ISBN: (纸本)9798350349092

Gas vesicles (GVs), gas-filled protein-encapsulated nanostructures, synthesized by microorganisms such as Halobacterium salinarum NRC-1 (HALO) and Anabaena flos aquae (ANA) have been reported as potential ultrasound contrast agents (UCA) due to their ability to mechanically vibrate with the same frequency, or harmonics, as the one from the incident ultrasound (US) wave. Despite GVs resonating in frequencies above 21 MHz, they collapse irreversibly when the US incident field pressure exceeds the vesicle collapse pressure. There are few reports on the relationship between the pressure of the acoustic wave incident in the GVs and the corresponding intensity of the backscattered wave signal from the GVs and, therefore, this study was designed to investigate how varying the relative incident US power, at 21MHz, reflects on the intensity of the US backscattered signal and the collapse of HALO and ANA GVs. The mean (±SD) of the grey level intensity for the ROIs, in maximum intensity pixel images, with GVs and PBS, were determined for transmitted acoustic relative power of 1, 5, 10, 20%. ANA-GVs presented a lower signal intensity, but greater resistance to the increase in relative US pressure. In conclusion, the best behavior was for the Halo-GVs at 5% power and at 21MHz frequency.

关键词： Resistance Proteins Frequency synthesizers Ultrasonic imaging Microorganisms Resonant frequency Harmonic analysis

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IIWM (Integration ImageJ with MATLAB) program for Segmentation and Volume Estimation in Ultrasound Biomicroscopic Images of Mouse Colon Tumor

IIWM (Integration ImageJ with MATLAB) Program for Segmentati...

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Ultrasonics Symposium (LAUS), IEEE UFFC Latin America

作者： Igor Soares Colonna Yasmin Farias Romi de Sant'Anna Rodrigo Pereira de Oliveira João Carlos Machado Biomedical Engineering Program/COPPE Federal University of Rio de Janeiro Rio de Janeiro Brazil INFNET Institute Rio de Janeiro Brazil Department of Surgery Biomedical Engineering Program/COPPE and Post-Graduation Program in Surgical Sciences School of Medicine Federal University of Rio de Janeiro Rio de Janeiro Brazil

ISBN: (数字)9798350349085

ISBN: (纸本)9798350349092

This article proposes the creation, implementation and development of an integration plugin for ImageJ that makes calls in MATLAB functions for the segmentation UBM imagens, more specific endoluminal (eUBM) e transabdominal (tUBM), in the laboratory the images are generating with frequencies between 30-40 MHZ. These images allow view colon layer, identify tumors in colons, to calculate tumor volume and 3D visualization. This integration may to improve the time taken to perform the procedure and the user experience to reduce the number of tasks and change windows, making the experience more fluid and with less risk of errors.

关键词： Solid modeling Image segmentation Visualization Ultrasonic imaging Three-dimensional displays Biological system modeling Volume measurement

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Bioengineered gas vesicle nanostructure increases the ultrasound backscattered signal amplitude in presence of tumoral enzyme Cathepsin B

Bioengineered gas vesicle nanostructure increases the ultras...

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IEEE Symposium (IUS) Ultrasonics

作者： Felipe Vianna Garrute Ana Beatriz F. Pacheco George Lu João Carlos Machado Biomedical Engineering Program/COOPE Federal University of Rio de Janeiro Rio de Janeiro Brazil Biophysics Institute Carlos Chagas Filho Federal University of Rio de Janeiro Rio de Janeiro Brazil Bioscience Research Collaborative Willian Rice University Houston USA Biomedical Engineering Program/COOPE and Post-Graduation Program in Surgical Sciences Federal University of Rio de Janeiro Rio de Janeiro Brazil

Gas vesicles (GVs), nanostructure gas-filled protein-shelled compartments, act as ultrasound contrast agents (UCAs). Recent works report bioengineered GvpC, one of the shell proteins, potentializes interactions with specific molecules in tumor microenvironment. An important target of investigation is Cathepsin B (CTSB), an enzyme produced by several tumors. CTSB acts in the tumoral microenvironment to increase tumor growth and invasion. This work objective relied on bioengineering the GVs to promote the GvpC-CTSB interaction with a subsequent increase of ultrasound (US) signal backscattered. GvpC was engineered to contain a known CTSB cleavage sequence re-added in striped GV (ΔGV). Western Blot (WB) test was used to compare the GVwt (wild type) and GV* (GV engendered) before and after 24 hours reaction with CTSB. US images for PBS (negative control), GVtw, ΔGV, GV* and CTSB+GV*, were obtained in 21MHz (5% total power) and regions-of-interest (ROIs) over the final B-mode image were determined. The WB test for GVwt resulted in mean (±SD) GvpC concentrations of 25169 (±8574) and 32873 (±9113) and GV* was 32487 (±6867) and 12763 (±2846), at t0 and t24, respectively. The US image gray level intensities for the ROIs related to the samples of PBS, GVwt, ΔGV, GV* and CTSB+GV* were 55.8 (±17.6), 47.4 (±16.6), 46.1 (±22.8) and 150.2 (±24), respectively. These results reveal an increase in the US signal backscattered from GV* after CTSV reaction. This increase is likely related to a direct GvpC degradation by CTSB, without affecting the GV* structure. In conclusion, high frequency US B-mode images showed the GV* with the potential to act as an UCA sensitive to the CTSB presence in tumoral microenvironment.

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In Vivo and Simultaneously Acquired Endoluminal Ultrasound Biomicroscopic and Colonoscopic Images of Inflamed Mouse Colon and Wall Thickness Measurement

In Vivo and Simultaneously Acquired Endoluminal Ultrasound B...

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IEEE International Ultrasonics Symposium

作者： Rodrigo P. de Oliveira Carine Belau de Castro Anderson Weber Faletti Cunha Rossana Colla Solleti Helena Lobo Borges Joao C. Machado Biomedical Engineering Program Federal University of Rio de Janeiro Rio de Janeiro Brazil Institute of Biomedical Science Federal University of Rio de Janeiro Rio de Janeiro Brazil Federal University of Rio Grande do Sul Tramandaí RS Brazil Biomedical Engineering Program/Post-Graduation Program on Surgical Sciences Federal University of Rio de Janeiro Rio de Janeiro Brazil

ISBN: (数字)9781728154480

ISBN: (纸本)9781728154497

Colon cancer has a high incidence, without considering skin cancer, being the third most common cancer in the world. Despite this high incidence and high mortality rate, most of the outcomes could be prevented by using accurate techniques for early cancer detection to enable early treatment. In this context, it is important to conduct preclinical studies, using animal models of colon diseases, to understand the development of the disease and to test new diagnostic approaches including imaging instrumentation. We have previously used a 3-French ultrasound mini-probe to image the layered structure in the mouse colon, to detect polyps, and to visualize their invasion through the colon wall. The present work aimed to measure the colon wall thickness and perform a numeric differentiation between the thickness of normal and inflamed mouse colon, a crucial step to elucidate the disease progression in colorectal cancer mouse model.

关键词： Visualization Ultrasonic imaging Ultrasonic variables measurement Mice Colon Thickness measurement Skin cancer

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Correction to: 3D-printed microgels supplemented with dentin matrix molecules as a novel biomaterial for direct pulp capping

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Clinical oral investigations 2023年第4期27卷 1799-1800页

作者： Diana Cunha Nayara Souza Manuela Moreira Nara Rodrigues Paulo Silva Cristiane Franca Sivaporn Horsophonphong Ashley Sercia Ramesh Subbiah Anthony Tahayeri Jack Ferracane Pamela Yelick Vicente Saboia Luiz Bertassoni Post-Graduation Program in Dentistry Federal University of Ceara Fortaleza Ceara Brazil. School of Dentistry Faculty of Pharmacy Dentistry and Nursing Federal University of Ceara Fortaleza Ceara Brazil. School of Dentistry University of Fortaleza Fortaleza Ceara Brazil. Division of Biomaterials and Biomechanics Department of Restorative Dentistry School of Dentistry Oregon Health & Science University Portland OR USA. Department of Pediatric Dentistry Faculty of Dentistry Mahidol University Salaya Thailand. Department of Orthodontics School of Medicine School of Engineering Tufts University Boston MA 02111 USA. Department of Restorative Dentistry Faculty of Pharmacy Dentistry and Nursing Federal University of Ceara Fortaleza Ceara Brazil. Division of Biomaterials and Biomechanics Department of Restorative Dentistry School of Dentistry Oregon Health & Science University Portland OR USA. bertasso@ohsu.edu. Center for Regenerative Medicine School of Medicine Oregon Health & Science University Portland OR USA. bertasso@ohsu.edu. Department of Biomedical Engineering School of Medicine Oregon Health & Science University Portland OR USA. bertasso@ohsu.edu. Cancer Early Detection Advanced Research Center (CEDAR) Knight Cancer Institute Portland OR USA. bertasso@ohsu.edu.

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Author Correction: A possible association between fructose consumption and pulmonary emphysema

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Scientific reports 2020年第1期10卷 16158页

作者： Camila Liyoko Suehiro Alessandra Choqueta de Toledo-Arruda Rodolfo de Paula Vieira Francine Maria de Almeida Clarice Rosa Olivo Milton de Arruda Martins Chin Jia Lin Laboratory of Molecular Pathology (LIM-22) Department of Pathology School of Medicine University of Sao Paulo Sao Paulo Brazil. Department of Medicine (LIM-20) School of Medicine University of Sao Paulo Sao Paulo Brazil. Federal University of Sao Paulo (UNIFESP) Post-Graduation Program in Sciences of Human Movement and Rehabilitation Santos - SP Brazil. Universidade Brasil Post-Graduation Program in Bioengineering and in Biomedical Engineering Sao Paulo - SP Brazil. Brazilian Institute of Teaching and Research in Pulmonary and Exercise Immunology (LABPEI) Sao Jose dos Campos - SP Brazil. Anhembi Morumbi University School of Medicine Sao Jose dos Campos - SP Brazil. Laboratory of Molecular Pathology (LIM-22) Department of Pathology School of Medicine University of Sao Paulo Sao Paulo Brazil. cjlin@usp.br.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

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Montelukast, Leukotriene Inhibitor, Reduces LPS-Induced Acute Lung Inflammation and Human Neutrophil Activation

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Archivos de Bronconeumología (English Edition) 2019年第11期55卷 573-580页

作者： Jaime Eduardo Davino-Chiovatto Manoel Carneiro Oliveira-Junior BreAnne MacKenzie Alana Santos-Dias Ana Roberta Almeida-Oliveira Jefferson Comin Jonco Aquino-Junior Auriléia Aparecida Brito Nicole Cristine Rigonato-Oliveira Nilsa Regina Damaceno-Rodrigues Ana Paula Ligeiro Oliveira Alessandro Pereira Silva Fernanda Marciano Consolim-Colombo Flavio Aimbire Hugo Caire Castro-Faria-Neto Rodolfo Paula Vieira Nove de Julho University (UNINOVE) São Paulo SP Brazil Brazilian Institute of Teaching and Research in Pulmonary and Exercise Immunology (IBEPIPE) São José dos Campos SP Brazil University of São Paulo School of Medicine Department of Pathology (LIM 59) São Paulo SP Brazil Post-graduation Program in Biomedical Engineering University of Mogi das Cruzes Mogi das Cruzes SP Brazil Federal University of Sao Paulo (UNIFESP) São José dos Campos SP Brazil Laboratory of Immunopharmacology Osvaldo Cruz Institute (IOC) Osvaldo Cruz Foundation Rio de Janeiro RJ Brazil Universidade Brasil Post-graduation Program in Bioengineering and in Biomedical Engineering São Paulo SP Brazil Federal University of Sao Paulo (UNIFESP) Post-graduation Program in Sciences of Human Movement and Rehabilitation Santos SP Brazil Anhembi Morumbi University School of Medicine Avenida Deputado Benedito Matarazzo 4050 São José dos Campos SP Brazil

Objectives Some pro-inflammatory lipids derived from 1 lipooxygenase enzyme are potent neutrophil chemoattractant, a cell centrally involved in acute respiratory distress syndrome (ARDS); a syndrome lacking effective treatment. Considering the beneficial effects of the leukotriene receptor inhibitor, montelukast, on other lung diseases, whether montelukast attenuates inflammation in a mouse model of ARDS, and whether it reduces LPS stimulated activation of human neutrophils was investigated. Methods Thirty-five C57Bl/6 mice were distributed into control (PBS) + 24 h, LPS + 24 h (10 μg/mouse), control + 48 h, LPS + 48 h, and LPS 48 h + Montelukast (10 mg/kg). In addition, human neutrophils were incubated with LPS (1 μg/mL) and treated with montelukast (10 μM). Results Oral-tracheal administration of montelukast significantly attenuated total cells ( P < .05), macrophages ( P < .05), neutrophils ( P < .01), lymphocytes ( P < .001) and total protein levels in BAL ( P < .05), as well as IL-6 ( P < .05), CXCL1/KC ( P < .05), IL-17 ( P < .05) and TNF-α ( P < .05). Furthermore, montelukast reduced neutrophils ( P < .001), lymphocytes ( P < .01) and macrophages ( P < .01) in the lung parenchyma. In addition, montelukast restored BAL VEGF levels ( P < .05). LTB4 receptor expression ( P < .001) as well as NF-κB ( P < .001), a downstream target of LPS, were also reduced in lung parenchymal leukocytes. Furthermore, montelukast reduced IL-8 ( P < .001) production by LPS-treated human neutrophils. Conclusion In conclusion, montelukast efficiently attenuated both LPS-induced lung inflammation in a mouse model of ARDS and in LPS challenged human neutrophils. Objetivos Algunos lípidos proinflamatorios derivados de la enzima lipooxigenasa 1 son potentes quimioatrayentes de neutrófilos, un tipo celular con una implicación principal en el síndrome de distrés respiratorio agudo (SDRA),

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Tracing the shear modulus of blood plasma during clotting and based on acoustic radiation force induced vibration of an embedded sphere

Tracing the shear modulus of blood plasma during clotting an...

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2017 IEEE International Ultrasonics Symposium, IUS 2017

作者： Costa, Jose Francisco Parcero, Guilherrne Crossetti Machado, Joao Carlos Biomedical Engineering Program Federal University of Rio de Janeiro Rio de Janeiro Brazil Post-Graduation Program in Surgical Sciences Federal University of Rio de Janeiro Rio de Janeiro Brazil

ISBN: (纸本)9781538633830

Blood clotting, a process to avoid bleeding, involves vasoconstriction, platelet plug build-up, coagulation, clot retraction and fibrinolysis. During coagulation, blood changes from liquid to gel, and the clot viscoelastic properties continue modifying until fibrinolysis. Tests to analyze bleeding disorders and anticoagulant therapy include clot-based assays, to detect the end-point of fibrin network in a plasma sample, and viscoelastic methods to trace the kinetics of whole blood coagulation. Ultrasound (US) methods are reported to measure clotting times, similar to clot-based assay findings, and shear modulus, μ, and viscosity, η, of the formed clot. This work objective was to measure μ, during plasma clotting, based on the vibration signature induced by impulsive acoustic radiation force (iARF) on an embedded sphere. © 2017 IEEE.

关键词： Coagulation Plasmas Acoustics Force Vibrations Hemorrhaging

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