One of the major challenges facing the emerging field of proteomics research is related to the technical difficulties in analyzing protein structure and function on a genomic scale. The routine purification of protein...
详细信息
One of the major challenges facing the emerging field of proteomics research is related to the technical difficulties in analyzing protein structure and function on a genomic scale. The routine purification of protein complexes as a means to investigate protein-protein interaction networks is of particularly high interest because of its significant potential to improve overall understanding of protein function and to improve ongoing drug discovery efforts. Automation of currently practiced laboratory procedures has the potential to markedly improve protein purification throughput, but important technical issues remain to be addressed. This paper investigates key bottlenecks in the automation of standard affinity-based procedures for protein complex purification and introduces a promising conceptual design for an automated workcell that would allow for rapid and efficient magnetic bead-based purification of protein complexes from model organisms suitable for a medium-sized research laboratory setting. The design specifications are based on a modular and flexible design that will permit routine, unattended batch isolation and processing of protein complexes from microbes.
Complete archaeal genomes were probed for the presence of long (≥ 25 bp) oligonucleotide repeats (words). We detected the presence of many words distributed in tandem with narrow ranges of periodicity (i.e., spacer l...
详细信息
Complete archaeal genomes were probed for the presence of long (≥ 25 bp) oligonucleotide repeats (words). We detected the presence of many words distributed in tandem with narrow ranges of periodicity (i.e., spacer length between repeats). Similar words were not identified in genomes of non-archaeal species, namely Escherichia coli, Bacillus subtilis, Haemophilus influenzae, Mycoplasma genitalium and Mycoplasma pneumoniae. BLAST similarity searches against the GenBank nucleotide sequence database revealed that these words were archaeal species-specific, indicating that they are of a signature character. Sequence analysis and genome viewing tools showed these repeats to be restricted to non-coding regions. Thus, archaea appear to possess a non-coding genomic signature that is absent in bacterial species. The identification of a species-specific genomic signature would be of great value to archaeal genome mapping, evolutionary studies and analyses of genome complexity.
Motivation: Gene chips or microarrays have made it possible to perform large-scale gene-expression experiments at the wholegenome level. To reduce the cost and identify genes that are differentially expressed between ...
详细信息
＜正＞Most of the molecular, cellular, and developmental biology researches focus on studying the mechanism behind a biological phenomenon. The center of the above researches is thus a pathway-discovery and a pathway ...
＜正＞Most of the molecular, cellular, and developmental biology researches focus on studying the mechanism behind a biological phenomenon. The center of the above researches is thus a pathway-discovery and a pathway interaction problem. Most of the publicly available pathway databases use hand-drawing picture to
A revised proof is given that the root-mean -square deviation between more than two vector sets after optimal superposition induces a metric. This corrects an error in a previous manuscript [Kaindl & Steipe (1997)...
详细信息
Bayesian networks are an attractive modeling tool for human sensing, as they combine an intuitive graphical representation with efficient algorithms for inference and learning. Temporal fusion of multiple sensors can ...
详细信息
We present preliminary results on training finite state machines (FSMs) as good/bad classifiers for polymerase chain reaction (PCR) primers. Novel features of the work presented include hybridization of multiple popul...
详细信息
Cyclooxygenases (COXs) are the primary targets of aspirin and other non-steroidal anti-inflammatory drugs (NSAIDs), and thus enzymes of major interest to pharmacology, pharmacogenetics, and epidemiology. Genetic varia...
详细信息
Cyclooxygenases (COXs) are the primary targets of aspirin and other non-steroidal anti-inflammatory drugs (NSAIDs), and thus enzymes of major interest to pharmacology, pharmacogenetics, and epidemiology. Genetic variants that affect enzyme function, or the interaction with NSAIDs, could alter drug response. We have screened the human COX1 gene coding regions of 48 African-American and 47 Caucasian individuals using DNA sequencing. We identified 13 coding-region variants, of which seven were amino-acid substitutions, and further five intronic polymorphisms within 60bp of an exon. All nonsynonymous variants were confirmed in an independent Caucasian population (n=94 unrelated individuals). Most of the discovered polymorphisms were rare, although some variants resulting in amino-acid changes occurred at appreciable frequency in at least one population (> or =4%: R8W, P17L, L237M). We used two sequence-homology-based software programs to predict the potential impact of these polymorphisms on COX1 function. The L237M substitution was predicted as most likely to alter protein function, whereas the glycine at position 230 may be specific to COX1 function. More detailed phenotypic characterizations of these COX1 polymorphisms remain to be undertaken. Copyright 2002 Wiley-Liss, Inc.
We present a method of using a genetic algorithm to evolve controls for a greedy algorithm. We demonstrate the technique on the location of embeddable DNA markers for genetic libraries. The technique has the potential...
详细信息
We present a method of using a genetic algorithm to evolve controls for a greedy algorithm. We demonstrate the technique on the location of embeddable DNA markers for genetic libraries. The technique has the potential for broad application and other applications are discussed.
暂无评论