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Ultrasensitive and selective electrochemical detection of hydroquinone using electropolymerized 7-amino cephalosporanic acid modified GCE

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Surface and Coatings Technology 2025年 512卷

作者： Sathya, H. Varatharajan, Pandiaraja Ashkar, M.A. Chandhru, M. Govindasamy, Mani Rani, S. Kutti Vasimalai, N. Department of Chemistry B.S. Abdur Rahman Crescent Institute of Science and Technology Chennai Vandalur600 048 India International Ph.D. Program in Innovative Technology of Biomedical Engineering and Medical Devices Ming Chi University of Technology New Taipei City243303 Taiwan Department of Physics B.S. Abdur Rahman Crescent Institute of Science and Technology Chennai Vandalur600 048 India Tamil Nadu Chennai602105 India Research Center for Intelligent Medical Devices Ming Chi University of Technology New Taipei City243303 Taiwan

Industrial wastewater pollution harms ecosystems and hinders sustainable development growth. Particularly, hydroquinone (HQ) has transitioned from a commercial chemical to an environmental hazard because of its extensive use in beauty products, pharmaceuticals, chemicals, and food industries. Therefore, developing electrochemical sensors for the detection of HQ is very important for environmental safety. Herein, the 7-ACA monomer was electropolymerized on the glassy carbon electrode (GCE) surface to examine the electrochemical oxidation of HQ utilizing CV (cyclic voltammetry) and DPV (differential pulse voltammetry) techniques. Scanning electron microscopy (SEM) characterization verified the deposition of the p-7-ACA on the glassy carbon electrode (GCE) surface. The designed p-7-ACA@GCE sensor exhibited better electrocatalytic properties. The acquired DPV observations for the p-7-ACA@GCE demonstrated excellent limit of detection (LOD) 8.86 nmol L−1 and broad linear range (0.33 μM to 955 μM) for the detection of HQ. The developed sensor exhibits excellent repeatability, reproducibility, selectivity and better cyclic stability. In addition, the fabricated p-7-ACA@GCE was used to determine the HQ in a different environmental water sample. © 2025

关键词： Cyclic voltammetry

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Self-packaged stretchable printed circuits with ligand-bound liquid metal particles in elastomer

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Nature Communications 2025年第1期16卷 1-11页

作者： Seo, Hyeonyeob Lee, Gun-Hee Park, Jiwoo Kim, Dong-Yeong Son, Yeonzu Kim, Semin Nam, Kum Seok Yang, Congqi Won, Joonhee Bae, Jae-Young Kim, Hyunjun Kang, Seung-Kyun Park, Steve Kang, Jiheong Park, Seongjun Department of Bio and Brain Engineering Korea Advanced Institute of Science and Technology (KAIST) Daejeon South Korea Departments of Cogno-Mechatronics Engineering Pusan National University Busan South Korea Departments of Optics and Mechatronics Engineering Pusan National University Busan South Korea School of Electrical Engineering Korea Advanced Institute of Science and Technology (KAIST) Daejeon South Korea Program of Brain and Cognitive Engineering Korea Advanced Institute of Science and Technology (KAIST) Daejeon South Korea Graduate School of Semiconductor Technology Korea Advanced Institute of Science and Technology (KAIST) Daejeon South Korea Medical Research Center Seoul National University Seoul South Korea Department of Materials Science and Engineering Korea Advanced Institute of Science and Technology (KAIST) Daejeon South Korea Department of Materials Science and Engineering Seoul National University Seoul South Korea KAIST Institute for NanoCentury Daejeon South Korea Department of Chemistry Seoul National University Seoul South Korea Department of Biomedical Sciences College of Medicine Seoul National University Seoul South Korea School of Transdisciplinary Innovations Seoul National University Seoul South Korea Interdisciplinary Program in Bioengineering College of Engineering Seoul National University Seoul South Korea Department of Transdisciplinary Medicine Seoul National University Hospital Seoul South Korea

Packaging in stretchable electronics is crucial to protect components from environmental damage while preserving mechanical flexibility and providing electrical insulation. The conventional packaging process involves multiple steps that increase in complexity as the number of circuit layers multiply. In this study, we introduce a self-packaged stretchable printed circuit board enabled by the in situ phase separation of liquid metal particles (LMPs) within various polymer matrices during solution-based printing processes. The ligand-bound LMPs (LB-LMPs), engineered to inhibit oxide growth, undergo in situ sintering, prompting vertical phase separation. This synthesis strategy not only achieves high initial conductivity of the LMPs but also encapsulates them within the polymer matrix, preventing leakage and providing electrical insulation. Our method enables multi-layer circuit printing, eliminating the need for additional activation and packaging processes. Furthermore, by integrating conductive materials into packaging layers for selective electrical conductivity, vertical interconnect accesses and conductive pads can be formed, enabling large-scale, stretchable, and leakage-free multi-layer electrical circuits and bio-interfaces. © The Author(s) 2025.

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Characterization of Fluoride-added Hydroxyapatite Derived from Eggshells for Dental Application

Characterization of Fluoride-added Hydroxyapatite Derived fr...

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biomedical engineering International Conference (BMEiCON)

作者： Vilasinee Uthayaphamornwat Anak Khantachawana Mettaya Kitiwan Kasama Srirussamee Phacharaphon Tunthawiroon Department of Biomedical Engineering School of Engineering King Mongkut’s Institute of Technology Ladkrabang (KMITL) Bangkok Thailand Department of Mechanical Engineering Faculty of Engineering King Mongkut’s University of Technology Thonburi (KMUTT) Bangkok Thailand Biological Engineering Program Faculty of Engineering King Mongkut’s University of Technology Thonburi (KMUTT) Bangkok Thailand Department of Physics School of Science King Mongkut’s Institute of Technology Ladkrabang (KMITL) Bangkok Thailand Devices and Systems for Energy and Environment Research Unit School of Science King Mongkut’s Institute of Technology Ladkrabang (KMITL) Bangkok Thailand Department of Industrial Engineering School of Engineering King Mongkut’s Institute of Technology Ladkrabang (KMITL) Bangkok Thailand

Hydroxyapatite (HA) is one of the potential biomaterials used for treating dental defects. It can be incorporated with fluoride to enhance its anti-caries properties, though other properties could be dependent on processing technique. Hence, this study aims to characterize the influence of sintering on the properties of fluoride-added HA derived from eggshells. FTIR and XRD techniques are used to characterize the chemical and crystal structures of the samples, whilst dissolution test is carried out to characterize material degradation in the acidic environment. The results show that sintering increased the crystallinity in both HA and fluoride-add HA (F-HA) samples, which corresponds with the enhanced stability during the dissolution test. Moreover, it is also found that the addition of fluoride could further stabilize the material structure, though the impurities found in all the samples still need to be minimized. These findings suggest that sintering and fluoride addition could potentially enhance anti-caries properties of eggshell-derived HA, which could be beneficial for dental application in future subject to further process optimization and analysis.

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Metal ratio and bimetal nanoarchitectonics of ammonia-based fluoride complex induced nickel hydroxide and manganese oxide composites as active materials of an energy storage device

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Journal of Energy Storage 2024年 80卷

作者： Hsu, Chuan-Chih Yu, You Zher Wu, Chung-Hsien Lee, Pin-Yan Chen, Hung-Ming Husain, Sadang Kongvarhodom, Chutima Hsiao, Yu-Cheng Lin, Lu-Yin Division of Cardiovascular Surgery Department of Surgery School of Medicine College of Medicine Taipei Medical University Taiwan Division of Cardiovascular Surgery Department of Surgery Taipei Medical University Hospital Taiwan Department of Chemical Engineering and Biotechnology National Taipei University of Technology Taipei Taiwan Gingen Technology Co. LTD. Rm. 7 10F. No.189 Sec. 2 Keelung Rd. Xinyi Dist. Taipei11054 Taiwan Department of Physics Faculty of Mathematics and Natural Science Lambung Mangkurat University Banjarmasin70124 Indonesia Department of Chemical Engineering King Mongkut's University of Technology Thonburi 126 Pracha-u-thit Toong-kru Bangkok10140 Thailand Department of Chemical Engineering Ming Chi University of Technology New Taipei City24301 Taiwan Cell Physiology and Molecular Image Research Center Wan Fang Hospital Taipei Medical University Taipei11696 Taiwan International PhD Program in Biomedical Engineering College of Biomedical Engineering Taipei Medical University Taipei11031 Taiwan Stanford Byers Center for Biodesign Stanford University CA94305-5428 United States Graduate Institute of Biomedical Optomechatronics College of Biomedical Engineering Taipei Medical University Taipei11031 Taiwan

Nickel and manganese compounds are widely applied as efficient battery-type active materials for energy storage devices, due to high theoretical capacities and abundant redox states for generating redox reactions to store charges. Morphology plays significant roles on energy storage ability. Structure directing agent (SDA) is widely reported to have ability for modulating configurations of active materials and to achieve excellent surface properties. In this work, it is the first time to use NH4BF4 and NH4HF2 as structure directing agents for synthesizing nickel manganese compounds derived from ZIF67 as active materials of energy storage devices. Bimetallic compounds are composed of nickel hydroxide and manganese oxide, which presents the favorable two-dimensional flower-like structure with high surface area and abundant redox states. The largest specific capacitance (CF) of 1358.2 F/g corresponding to the capacity of 226.4 mAh/g are achieved for the bimetallic compound synthesized using the Ni to Mn precursor ratio of 1 to 1 (NiMn11). The energy storage device assembled using NiMn11 and reduced graphene oxide on Ni foams presents a maximum energy density of 11.45 Wh/kg at the power density of 0.35 kW/kg, and excellent long-term stability with the CF retention of 85.6% and Coulombic efficiency of 87.5% after 10,000 cycles. © 2023

关键词： Fluorine compounds

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An enzyme-free and ultrafast cell-dissociation technique for cell culture applications using acoustofluidics 24

An enzyme-free and ultrafast cell-dissociation technique for...

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24th International Conference on Miniaturized Systems for Chemistry and Life sciences, MicroTAS 2020

作者： Salari, Alinaghi Appak-Baskoy, Sila Coe, Imogen R. Tsai, Scott S.H. Kolios, Michael C. Canada Biomedical Engineering Graduate Program Ryerson University Canada Department of Chemistry and Biology Ryerson University Canada Molecular Science Graduate Program Ryerson University Canada Department of Mechanical and Industrial Engineering Ryerson University Canada Department of Physics Ryerson University Canada

ISBN: (纸本)9781733419017

Cell-culture techniques are widely used for in-vitro drug screening and biochemical analyses. The classical models for dissociating cell monolayers into single-cell suspensions include enzymatic techniques (e.g., trypsinization). All available enzymatic and non-enzymatic techniques require long treatment duration, typically on the order of minutes (5-10 min). Here, we report a novel, ultrafast, and high-throughput technique, using acoustofluidics, that enables the detachment of adhered cells into suspensions within seconds, while maintaining cellular viability similar to that of the trypsinization method. In our technique, only the adhered cells experience the acoustic waves, minimizing any detrimental effects of the dissociating agent on the detached cells. © 2020 CBMS-0001

关键词： Cells

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High throughput intracellular delivery facilitated by acoustofluidics 24

High throughput intracellular delivery facilitated by acoust...

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24th International Conference on Miniaturized Systems for Chemistry and Life sciences, MicroTAS 2020

作者： Salari, Alinaghi Appak-Baskoy, Sila Coe, Imogen R. Abousawan, John Antonescu, Costin N. Tsai, Scott S.H. Kolios, Michael C. Canada Biomedical Engineering Graduate Program Ryerson University Canada Department of Chemistry and Biology Ryerson University Canada Molecular Science Graduate Program Ryerson University Canada Department of Mechanical and Industrial Engineering Ryerson University Canada Department of Physics Ryerson University Canada

ISBN: (纸本)9781733419017

Many cell-based therapies rely on the successful delivery of cargo materials into cells. Here, we present a novel in vitro strategy for intracellular delivery, which is based on adherent cells exposed to acoustic waves. Strong acoustic waves mediated through a substrate inside a microfluidic channel cause the adherent cells to oscillate. As a result of continuous cell oscillation and cell-induced microstreaming, successful delivery of different size dextrans into MDA-MB-231 cells (with delivery efficiencies of 55-85%) is demonstrated. This novel acoustofluidic method achieves rapid and scalable delivery of cargo materials into cells and thus may be preferred over lipofection, electroporation, or other delivery methods. © 2020 CBMS-0001

关键词： Cells

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Desialylated Platelet Clearance in the Liver is a Novel Mechanism of Systemic Immunosuppression

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Research 2024年第3期2023卷 35-50页

作者： June Li Danielle Karakas Feng Xue Yingyu Chen Guangheng Zhu Yeni H.Yucel Sonya A.MacParland Haibo Zhang John W.Semple John Freedman Qizhen Shi Heyu Ni Department of Laboratory Medicine and Pathobiology University of TorontoTorontoONCanada Toronto Platelet Immunobiology Group TorontoONCanada Keenan Research Centre for Biomedical Science of St.Michael's Hospital TorontoONCanada Canadian Blood Services Centre for Innovation TorontoONCanada Departments of Pediatrics Medical College of WisconsinMilwaukeeWIUSA Blood Research Institute Versiti WisconsinMilwaukeeWIUSA Departments of Ophthalmology and Vision Sciences Medicine University of TorontoTorontoONCanada Faculty of Engineering and Architectural Science Ryerson UniversityTorontoONCanada Multi-Organ Transplant Program Toronto General Hospital Research InstituteTorontoONCanada Department of Immunology University of TorontoTorontoONCanada Critical Care Medicine Department ofAnesthesiology and PainUniversity of TorontoTorontoONCanada Department of Physiology University of TorontoTorontoONCanada Department of Pharmacology University of TorontoTorontoONCanada. Division of Hematology and Transfusion Medicine Lund UniversityLundSweden clinical Immunology and Transfusion Medicine Ofice of Medical ServicesRegion SkaneLundSweden Department of Medicine University of TorontoTorontoONCanada Children's Research Institute Children's WisconsinWauwatosaWIUSA Midwest Athletes Against Childhood Cancer Fund Research Center MilwaukeeWIUSA.

Platelets are small, versatile blood cells that are critical for hemostasis/thrombosis. Local platelet accumulation is a known contributor to proinflammation in various disease states. However, the anti-inflammatory/immunosuppressive potential of platelets has been poorly explored. Here, we uncovered, unexpectedly, desialylated platelets (dPLTs) down-regulated immune responses against both platelet-associated and -independent antigen challenges. Utilizing multispectral photoacoustic tomography, we tracked dPLT trafficking to gut vasculature and an exclusive Kupffer cell-mediated dPLT clearance in the liver, a process that we identified to be synergistically dependent on platelet glycoprotein Ibα and hepatic Ashwell–Morell receptor. Mechanistically, Kupffer cell clearance of dPLT potentiated a systemic immunosuppressive state with increased anti-inflammatory cytokines and circulating CD4+ regulatory T cells, abolishable by Kupffer cell depletion. Last, in a clinically relevant model of hemophilia A, presensitization with dPLT attenuated anti-factor VIII antibody production after factor VIII ( infusion. As platelet desialylation commonly occurs in daily-aged and activated platelets, these findings open new avenues toward understanding immune homeostasis and potentiate the therapeutic potential of dPLT and engineered dPLT transfusions in controlling autoimmune and alloimmune diseases.

关键词： Platelet transfusion homeostasis

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BPS2025 - Inferring single-cell dynamics of a molecular reporter from unlabeled live-cell light microscopy data analyzed with delay embedding

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Biophysical Journal 2025年第3期124卷 205a-205a页

作者： Jalim Singh Jeremy T. Copperman Laura M. Heiser Daniel M. Zuckerman Biomedical Engineering Program Oregon Health and Science University Portland OR USA Cancer Early Detection Advanced Research Center Oregon Health and Science University Portland OR USA Oregon Health and Science University Portland OR USA

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High-Order Cardiomyopathy Human Heart Model and Mesh Generation

High-Order Cardiomyopathy Human Heart Model and Mesh Generat...

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2021 Computing in Cardiology, CinC 2021

作者： Mohammadi, Fariba Shontz, Suzanne M Linte, Cristian A Department of Mechanical Engineering University of Kansas LawrenceKS United States Department of Electrical Engineering and Computer Science University of Kansas LawrenceKS United States Bioengineering Program University of Kansas LawrenceKS United States Information and Telecommunication Technology Center University of Kansas LawrenceKS United States Chester F Carlson Center for Imaging Science Rochester Institute of Technology RochesterNY United States Department of Biomedical Engineering Rochester Institute of Technology RochesterNY United States

ISBN: (纸本)9781665479165

Faithful, accurate, and successful cardiac biomechanics and electrophysiological simulations require patient-specific geometric models of the heart. Since the cardiac geometry consists of highly-curved boundaries, the use of high-order meshes with curved elements would ensure that the various curves and features present in the cardiac geometry are well-captured and preserved in the corresponding mesh. Most other existing mesh generation techniques require computer-aided design files to represent the geometric boundary, which are often not available for biomedical applications. Unlike such methods, our technique takes a high-order surface mesh, generated from patient medical images, as input and generates a high-order volume mesh directly from the curved surface mesh. In this paper, we use our direct high-order curvilinear tetrahedral mesh generation method [1] to generate several second-order cardiac meshes. Our meshes include the left ventricle myocardia of a healthy heart and hearts with dilated and hypertrophic cardiomyopathy. We show that our high-order cardiac meshes do not contain inverted elements and are of sufficiently high quality for use in cardiac finite element simulations. © 2021 Creative Commons.

关键词： Heart

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Accelerating Real-Time Imaging for Radiotherapy: Leveraging Multi-GPU Training with PyTorch 22

Accelerating Real-Time Imaging for Radiotherapy: Leveraging ...

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22nd IEEE International Conference on Machine Learning and Applications, ICMLA 2023

作者： Obe, Ruth Kaufmann, Brandt Baird, Kaelen Kadel, Sam Soltani, Yasmin Cham, Mostafa Gobbert, Matthias K. Barajas, Carlos A. Jiang, Zhuoran Sharma, Vijay R. Ren, Lei Peterson, Stephen W. Poif, Jerimy C. U. of Houston-Clear Lake Dept. of Computer Science and of Software Engineering United States U. of San Francisco Dept. of Mathematics and Statistics United States Skidmore College Dept. of Computer Science and of Mathematics United States Mount Holyoke College Dept. of Computer Science and of Psychology United States U. of Houston Dept. of Biomedical Engineering United States U. of Maryland Dept. of Information Systems Baltimore County United States U. of Maryland Dept. of Mathematics and Statistics Baltimore County United States Medical Physics Graduate Program Duke University United States U. of Maryland School of Medicine Dept. of Radiation Oncology United States U. of Maryland School of Medicine Department of Radiation Oncology United States U. of Cape Town Dept. of Physics South Africa H3D Inc. United States

ISBN: (纸本)9798350345346

Proton beam therapy is an advanced form of cancer radiotherapy that uses high-energy proton beams to deliver precise and targeted radiation to tumors. This helps to mit-igate unnecessary radiation exposure in healthy tissues. Real-time imaging of prompt gamma rays with Compton cameras has been suggested to improve therapy efficacy. However, the camera's non-zero time resolution leads to incorrect interaction classifications and noisy images that are insufficient for accurately assessing proton delivery in patients. To address the challenges posed by the Compton camera's image quality, machine learning techniques are employed to classify and refine the generated data. These machine-learning techniques include recurrent and feedforward neural networks. A PyTorch model was designed to improve the data captured by the Compton camera. This decision was driven by PyTorch's flexibility, powerful capabilities in handling sequential data, and enhanced G PU usage. This accelerates the model's computations on large-scale radiotherapy data. Through hyperparameter tuning, the validation accuracy of our PyTorch model has been improved from an initial 7 % to over 60 %. Moreover, the PyTorch Distributed Data Parallelism strategy was used to train the RNN models on multiple G PU s, which significantly reduced the training time with a minor impact on model accuracy. © 2023 IEEE.

关键词： Clas-sification Compton camera Distributed Data Parallelism Proton beam therapy PyTorch Recurrent neural network

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