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Pueraria lobata (Kudzu) photosystem i improves the photoelectrochemical performance of silicon

Industrial Biotechnology 2013年第1期9卷 37-41页

作者： Gunther, Darlene Leblanc, Gabriel Cliffel, David E. Kane Jennings, G. Interdisciplinary Materials Science Graduate Program Vanderbilt University Nashville TN United States Department of Chemistry Vanderbilt University Nashville TN United States Department of Chemical and Biomolecular Engineering Vanderbilt University 2301 Vanderbilt Place Nashville TN 37235 United States

Photosystem I (PSI), a protein complex found within the thylakoid membrane of green plants and some bacteria, drives nature's 90-terawatt solar energy conversion process known as photosynthesis. Kudzu (Pueraria lobata) is an invasive vine from eastern Asia that grows in the southern United States, spreading at ∼500 km2 per year and causing extensive environmental damage. This article reports the first integration of nature's optimized solar energy converter-PSI from the fast-growing, invasive leaves of kudzu-with the ubiquitous platform of integrated circuits-silicon-to prepare a "wet" photoelectrochemical cell. Herein, we report on the success of PSI films extracted from the kudzu leaf and deposited on boron-doped silicon (p-Si) to enhance photoelectrochemical current over p-Si alone. © 2013, Mary Ann Liebert, Inc.

关键词： Solar energy

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High Speed Magnetic Tweezers at 10,000fps with Reflected Hg-Lamp Illumination

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2012 Annual Conference on Experimental and Applied Mechanics

作者： Lansdorp, Bob M. Saleh, Omar A. Materials Department University of California Santa Barbara Santa Barbara CA 93106 United States Materials Department and Biomolecular Science and Engineering Program University of California Santa Barbara Santa Barbara CA 93106 United States

ISBN: (纸本)9781461444350

The magnetic tweezer is a simple and stable single-molecule manipulation instrument. However, the standard probe-tracking methods have typically failed to reach the high resolution (∼0.3 nm) needed to measure motor protein stepping. In this paper we present a novel illumination geometry, based on an inverted microscope with Hg lamp illumination, that aims to push the resolution of magnetic tweezers to their ultimate thermal limits. Using a metal-coated coverslip and motorized magnets, we convert a standard inverted microscope into a high-resolution magnetic tweezers instrument. Our novel optical geometry reduces the restrictions on magnet design inherent to transmission-based illumination, and does not require fiber-optic coupling. We introduce a high-speed CMOS camera as the optical detector, and demonstrate how an improvement in temporal resolution directly impacts the spatial resolution. © The Society for Experimental Mechanics, Inc. 2013.

关键词： Magnets

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Immunotherapy Bridge 2016 and Melanoma Bridge 2016: meeting abstracts

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Journal of Translational Medicine 2017年第1期15卷 1-14页

作者： Vera Hirsh Sandro Pignata Melissa Bersanelli Donatello Gasparro Francesco Leonardi Sebastiano Buti Letizia Gnetti Cinzia Azzoni Lorena Bottarelli Enrico Maria Silini Erik Wennerberg Sandra Demaria Claire Vanpouille-Box Karsten Pilones Nils Rudqvist Julie Diamond Silvia Formenti Aranzazu Mediero Bruce Cronstein Zachary S. Morris Emily I. Guy David M. Francis Monica M. Gressett Eric A. Armstrong Shyhmin Huang Jacquelyn A. Hank Anna Hoefges Alexander L. Rakhmilevich Paul M. Harari Paul M. Sondel Stephen D. Gilles Alan J. Korman Yared Hailemichael Willem W. Overwijk Per thor Straten Alessandro Lugli Heather Dawson Annika Blank Inti Zlobec Luigi Fattore Susan Costantini Maria Elena Pisanu Paolo Antonio Ascierto Gennaro Ciliberto Debora Malpicci Mario Acunzo Giulia Romano Giovanni Nigita Carlo Maria Croce Alessandro Laganà Ciro Francesco Ruggiero Alessia Noto Claudia De Vitis Rita Mancini Michael Postow Jason Luke David Stroncek Luciano Castiello Wenjing Chen Ping Jin Jiaqiang Ren Marianna Sabatino Soldano Ferrone Connie PM Duong Marie Vetizou Laurence Zitvogel Marcella Occelli Carolina Cauchi Cristiana Lo Nigro Chiara Varamo Daniela Vivenza Silvia Violante Laura Lattanzio Marco Carlo Merlano Grazia Sciancalepore Giuseppe Forte Michela Rovera Zelda Seia Stefania Palazzini Fabiana Errico Davide Basso Laura Quaranta Fulvio Lavagna Paolo Bosio Duane Moogk Shi Zhong Victoria Fang Janna Dougherty Arianne Perez-Garcia Iman Osman Michelle Krogsgaard Zhiya Yu Nicholas P. Restifo Ivan Liadi Navin Varadarajan William Rittase Cheng Zhu Alan Frey Timea Balatoni Gabriella Liszkay Miklós Kasler Anita Moho Timea Sebestyén Anita Varga Judit Oláh Zsuzsanna Lengyel Gabriella Emri Andrea Ladányi Beatrice Polini Stefano Fogli Sara Carpi Maria Cristina Breschi Paola Nieri Barbara Pardini Alessio Naccarati Nevio Dubbini Antonella Romanini Francesca Morgese Davide Soldato Silvia Pagliaretta Riccardo Giampieri Silvia Rinaldi Mariangela Torniai Agnese Savini Azzurra Onofri Francesca Bianchi Rossana Berardi Donatella Brancorsini Anna Campanati McGill University Health Centre Cedars Cancer Centre Montreal QC Canada Dipartimento Uro-Ginecologico S.C. Oncologia Medica Uro-Ginecologica Istituto Nazionale Tumori Fondazione “G. Pascale” Napoli Italy Medical Oncology University Hospital of Parma Via Gramsci 14 43126 Parma PR Italy Anatomic Pathology Unit University Hospital of Parma Via Gramsci 14 43126 Parma PR Italy Weill Cornell Medicine Cornell University New York NY USA Department of Radiation Oncology Weill Cornell Medical College New York NY USA New York University Langone Medical Center New York NY USA Dept. of Human Oncology School of Medicine and Public Health University of Wisconsin Madison WI USA Provenance Biopharmaceuticals Carlisle MA USA Bristol-Myers Squibb Company Redwood City CA USA Department of Melanoma Medical Oncology The University of Texas MD Anderson Cancer Center Houston TX USA Department of Hematology Center for Cancer Immune Therapy (CCIT) University Hospital Herlev Copenhagen Denmark Department of Immunology and Microbiology University of Copenhagen Copenhagen Denmark Institute of Pathology University of Bern Bern Switzerland Istituto Nazionale per lo Studio e la Cura dei Tumori “Fondazione G. Pascale” Naples Italy Dipartimento di Chirurgia “P. Valdoni” Sapienza Università di Roma Rome Italy IRCCS Pascale Naples Italy Dipartimento Melanoma Tessuti molli Muscolo-Scheletrico e Testa-Collo Oncologia Medica Melanoma Immunoterapia Oncologica e Terapie Innovative Istituto Nazionale Tumori “G. Pascale” Via M. Semmola 80131 Naples Italy Dipartimento di Medicina Sperimentale e Clinica Università degli Studi di Catanzaro “Magna Graecia” Catanzaro Italy Department of Molecular Virology Immunology and Medical Genetics The Ohio State University Comprehensive Cancer Center Columbus OH USA Department of Genetics and Genomic Sciences Icahn School of Medicine at Mount Sinai New York NY USA Dipartimento di Medicina Clinica e Molecolare Sapienza Università di Roma Rome

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Photoluminescence: Dual Role of Blue Luminescent MoS2Quantum Dots in Fluorescence Resonance Energy Transfer Phenomenon (Small 19/2014)

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Small 2014年第19期10卷

作者： Hyun Dong Ha Dong Ju Han Jong Seob Choi Minsu Park Tae Seok Seo Department of Chemical and Biomolecular Engineering (BK21 program) and Institute for the BioCentury Korea Advanced Institute of Science and Technology (KAIST) 291 Daehak-ro Yuseong-gu Daejeon 305–701 Republic of Korea

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Accurate Zygote‐Specific Discrimination of Single‐Nucleotide Polymorphisms Using Microfluidic Electrochemical DNA Melting Curves†

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Angewandte Chemie 2014年第12期126卷

作者： Allen H. J. Yang Kuangwen Hsieh Adriana S. Patterson B. Scott Ferguson Michael Eisenstein Kevin W. Plaxco H. Tom Soh Department of Mechanical Engineering University of California Santa Barbara (USA) Department of Chemistry and Biochemistry and Biomolecular Science and Engineering Program University of California Santa Barbara (USA) Materials Department University of California Santa Barbara Santa Barbara CA 93106 (USA)

We report the first electrochemical system for the detection of single‐nucleotide polymorphisms (SNPs) that can accurately discriminate homozygous and heterozygous genotypes using microfluidics technology. To achieve this, our system performs real‐time melting‐curve analysis of surface‐immobilized hybridization probes. As an example, we used our sensor to analyze two SNPs in the apolipoprotein E ( ApoE ) gene, where homozygous and heterozygous mutations greatly affect the risk of late‐onset Alzheimer’s disease. Using probes specific for each SNP, we simultaneously acquired melting curves for probe–target duplexes at two different loci and thereby accurately distinguish all six possible ApoE allele combinations. Since the design of our device and probes can be readily adapted for targeting other loci, we believe that our method offers a modular platform for the diagnosis of SNP‐based diseases and personalized medicine.

关键词： DNA Elektrochemie Mikrofluidik Molekulare Diagnostik

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engineering Rhodobacter Sphaeroides for direct electron transfer by heterogenous expression of redoxactive components from electrogenic species

Engineering Rhodobacter Sphaeroides for direct electron tran...

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Food, Pharmaceutical and Bioengineering Division 2013 - Core programming Area at the 2013 AIChE Annual Meeting: Global Challenges for engineering a Sustainable Future

作者： Cheng, Danhui Wang, Wanyang Chow, King L. Hsing, I.-Ming Bioengineering Graduate Program Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong Division of Biomedical Engineering Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong Department of Chemical and Biomolecular Engineering Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong Division of Life Science Hong Kong University of Science and Technology Clear Water Bay Kowloon Hong Kong

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engineering Microorganisms for Biomedical and Energy Applications

Engineering Microorganisms for Biomedical and Energy Applica...

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第八届全国微全分析系统学术会议、第三届全国微纳尺度生物分离分析学术会议暨第五届国际微化学与微系统学术会议

作者： I-Ming Hsing Danhui Cheng Department of Chemical and Biomolecular Engineering the Hong Kong University of Science and TechnologyHong Kong SARChina Division of Biomedical Engineering and Bioengineering Graduate Programthe Hong Kong University of Science and Technology Division of Biomedical Engineering and Bioengineering Graduate Program the Hong Kong University of Science and Technology

The availability of technologies to rapidly synthesize and decode genetic sequences has provided new applications for recombinant DNA technology and genetic engineering developed in early *** new genetic engineering approach,also coined as synthetic biology by many scientists,often involves engineering of microorganism through expression of heterologous gene segments in the microbial *** ability to engineer microorganisms empowered with tailor-made characteristics has provided exciting opportunities in different areas of *** instance,drug synthesis and novel drug delivery methods based on engineered bacteria strains have been extensively studied in biomedical ***,various synthetic biology approaches have been proposed to construct novel metabolic pathways in order to achieve higher yield in bioenergy & biofuel production,such as in ethanol fermentation,hydrogen production and bioelectricity generation through a microbial fuel *** this plenary presentation,we will start with an overview of this very exciting technology (*** biology),followed by a detailed discussion of two related projects in my *** the first part,we utilized yeast display technology for developing a new cell-based immunoassay *** the second one,we engineered photosynthetic bacteria to rewire its indigenous electron transfer pathway in pursuit of converting solar energy to ***,for the interests of our microfluidic community,some thoughts and opportunities of studying and utilizing engineered microorganisms in a microfluidic platform will be discussed.

关键词： Synthetic Biology Genetic engineering Yeast Surface Display Photosynthetic Bacteria

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Click Chemistry: Click Chemistry in Materials science (Adv. Funct. Mater. 18/2014)

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Advanced Functional Materials 2014年第18期24卷

作者： Weixian Xi Timothy F. Scott Christopher J. Kloxin Christopher N. Bowman Department of Chemical and Biological Engineering Materials Science and Engineering Program University of Colorado Boulder Colorado 80309–0596 USA Department of Chemical Engineering University of Michigan Ann Arbor Michigan 48109–2136 USA Department of Materials Science & Engineering 150 Academy Street Newark Delaware 19716 USA Department of Chemical & Biomolecular Engineering University of Delaware 150 Academy Street Newark Delaware 19716 USA

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Erratum: “Power spectrum and Allan variance methods for calibrating single-molecule video-tracking instruments” [Rev. Sci. Instrum. 83, 025115 (2012)]

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Review of Scientific Instruments 2014年第1期85卷 019901-019901页

作者： Bob M. Lansdorp Omar A. Saleh 1Materials Department University of California Santa Barbara Santa Barbara California 93106 USA 2Materials Department and Biomolecular Science and Engineering Program University of California Santa Barbara Santa Barbara California 93106 USA

In the original paper1 we made an error in Eq. (18). The equation read

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Hydrophobic Surfaces: Trilevel‐Structured Superhydrophobic Pillar Arrays with Tunable Optical Functions (Adv. Funct. Mater. 35/2014)

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Advanced Functional Materials 2014年第35期24卷

作者： Sanghyuk Wooh Jai Hyun Koh Soojin Lee Hyunsik Yoon Kookheon Char The National Creative Research Initiative Center for Intelligent Hybrids School of Chemical & Biological Engineering The WCU Program of Chemical Convergence for Energy & Environment Seoul National University 1 Gwanak‐ro Gwanak‐gu Seoul 151–742 Korea Clean Energy Research Center Korea Institute of Science and Technology Seoul 136‐791 Korea Department of Chemical & Biomolecular Engineering Seoul National University of Science & Technology Seoul 139–743 Korea

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