A long-chain fatty acid is a regulatory signal that modulates gene expression. In this study, proteomic studies that compared global translational differences between Escherichia coli cells grown in the presence of ex...
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A long-chain fatty acid is a regulatory signal that modulates gene expression. In this study, proteomic studies that compared global translational differences between Escherichia coli cells grown in the presence of exogenous glucose and oleic acid were examined by two-dimensional gel electrophoresis (2-DE) and tandem mass spectrometry (LC-MS/MS). Of about 40 proteins showing altered expression levels with the presence of oleic acid, 10 including AldA, AceA, FadA, FadB, FadL, MalE, RbsB, Udp, Cdd and YccU were newly synthesized. Among them, the promoter of aldehyde dehydrogenase A (aldA promoter) was used for the production of a recombinant protein. Analysis of fluorescence intensities and confocal microscopic imaging revealed that aldA promoter is a very good promoter in that it is tightly controlled and highly expressed with active form compared with IPTG- inducible promoter. Thus the aldA promoter induced by oleic acid may be useful for achieving high-level expression of genes in E. coli.
MOTIVATION:The fear of hypoglycemia remains an important limiting factor in the ability of an individual with type 1 diabetes to tightly regulate glycemia. Continuous glucose monitors provide important feedback to imp...
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MOTIVATION:The fear of hypoglycemia remains an important limiting factor in the ability of an individual with type 1 diabetes to tightly regulate glycemia. Continuous glucose monitors provide important feedback to improve glycemic control, but there remains a need for these devices to better alarm of possible impending hypoglycemia, particularly overnight or other periods when the individual is engaged in activities that take their focus away from glucose monitoring.
METHODS:We have previously proposed an algorithm, based on the use of real-time glucose sensor signals and optimal estimation theory (Kalman filtering), to predict hypoglycemia; the algorithm was validated in simulation-based studies. In this article we further refine and validate the prediction algorithm based on the analysis of clinical hypoglycemic clamp data from 13 subjects. The sensitivity and specificity of the predictions are calculated with respect to reference blood glucose values obtained at the same sampling rate of the sensor.
RESULTS:For a 30-minute prediction horizon and alarm threshold of 70 mg/dl, the sensitivity and specificity were 90 and 79%, respectively, indicating that a 21% false alarm rate must be tolerated to predict 90% of the hypoglycemic events 30 minutes ahead of time. Shorter prediction horizons yield a significant improvement in sensitivity and specificity.
DISCUSSION:Sensitivity and specificity data as a function of prediction horizon and alarm threshold enable an individual to adjust the alarm to best meet their needs. Such decisions can be made depending on the subject's risk for hypoglycemia, for example.
Surface‐biofunctionalized synthetic polymer fibers composed of a fiber‐forming host polymer and an oligopeptide conjugate can be prepared from electrospinning, report Spontak and co‐workers on p. 87. The conjugate ...
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Surface‐biofunctionalized synthetic polymer fibers composed of a fiber‐forming host polymer and an oligopeptide conjugate can be prepared from electrospinning, report Spontak and co‐workers on p. 87. The conjugate consists of a polypeptide segment and a polymer block that is compatible with the host polymer. Because the more polarizable peptide segment migrates to the surface during electrospinning, peptide surface‐enrichment is achieved in a single step without further treatment.
A cell-based protein-detecting array was developed on which genetically engineered microbes were spatially organized as sensor elements in a microfluidic device. Whole E. coli cells displaying protein-binding peptide ...
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People with type 1 diabetes require frequent adjustment of their insulin dose to maintain as near normal ***glyoemia as possible. This process is not only burdensome, but form any ***difficult to achieve. As a result,...
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People with type 1 diabetes require frequent adjustment of their insulin dose to maintain as near normal ***glyoemia as possible. This process is not only burdensome, but form any ***difficult to achieve. As a result, control algorithms to facilitate the insulin dosage have been proposed, but have not been completely successful in normalizing glycemia. Here we present a novel run–to–run control algorithm to adjust the meal related insulin dose using only postprandial blood glucose measurements.
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