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Enhancing miso production with optimized puffed-rice koji and its biological function evaluation

Food Bioscience 2024年 60卷

作者： Lin, Shin-Ping Chou, Yu-Chieh Chen, Chiao-En Lai, Yen-Tso Hsieh, Chen-Che Tsai, Tsung-Yu Lin, Yun-Hsin Santoso, Shella Permatasari Lin, Hui-Wen Cheng, Kuan-Chen School of Food Safety Taipei Medical University 250 Wu-Hsing Street Taipei 11031 Taiwan Research Center of Biomedical Device Taipei Medical University 250 Wu-Hsing Street Taipei 11031 Taiwan TMU Research Center for Digestive Medicine Taipei Medical University 250 Wu-Hsing Street Taipei 11031 Taiwan Ph.D. Program in Drug Discovery and Development Industry College of Pharmacy Taipei Medical University 250 Wu-Hsing Street Taipei 11031 Taiwan Institute of Food Science and Technology National Taiwan University 1 Roosevelt Rd. Sec. 4 Taipei 10617 Taiwan Department of Food and Nutrition Providence University 200 Sec. 7 Taiwan Boulevard Shalu Dist. Taichung 43301 Taiwan Institute of Biotechnology National Taiwan University 1 Roosevelt Rd. Sec. 4 Taipei 10617 Taiwan Department of Food Science Fu Jen Catholic University 510 Zhongzheng Rd. Xinzhuang Dist. New Taipei City 242062 Taiwan Department of Chemical Engineering Widya Mandala Surabaya Catholic University 37 Jl. Kalijudan 37 East Java Surabaya 60114 Indonesia Collaborative Research Center for Zero Waste and Sustainability 37 Jl. Kalijudan East Java Surabaya 60114 Indonesia Department of Optometry Asia University 500 Lioufeng Rd. Wufeng Taichung 41354 Taiwan Department of Medical Research China Medical University Hospital China Medical University 91 Hsueh-Shih Road Taichung 40402 Taiwan

In order to enhance enzyme activities of koji and further improve the health benefits and nutritional value of miso, puffed rice was used to prepare koji with optimization of fermentation utilizing response surface methodology (RSM), and the optimized puffed rice koji was used to produce miso and low-salt miso. Results showed that β-glucosidase activity increased by 1.98-fold after RSM optimization. The optimized puffed rice koji also showed the highest β-amylase and protease activities (237.1 and 968.8 units/g, respectively) compared to rice koji (108.7 and 637.4 units/g, respectively). Moreover, miso made from puffed-rice koji presented a much higher polyphenol content, antioxidant activity, and aglycone conversion. These findings clarified the relationship between changes in enzyme activities in koji and the important components of miso, and also advance solid-state fermentation research and development of functional miso. © 2024

关键词： Aspergillus oryzae Koji Miso Puffed rice Solid-state fermentation β-glucosidase

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Yield quality and performance of line 13, 46 and 52 black rice from gamma ray irradiation

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IOP Conference Series: Earth and Environmental Science 2021年第1期905卷

作者： B Pujiasmanto A A Wicaksono Supriyono Nandariyah Sutarno Riyatun Suharyana Lecturer of Agrotechnology Study Program Faculty of Agriculture Sebelas Maret University Surakarta 57126 Central Java Indonesia Research and Development Center for Biotechnology and Biodiversity Research and Community Service Institute Universitas Sebelas Maret Indonesia Undergraduate Program in Agrotechnology Study Program Faculty of Agriculture Sebelas Maret University Surakarta 57126 Central Java Indonesia Lecturer of Department Faculty of Mathematics and Natural science of Sebelas Maret University Surakarta 57126 Central Java Indonesia

Black rice is a functional food plant with high fiber and anthocyanin content. It has a weakness that is a high content of amylose which causes rice texture to harden. The study used a different treatment of the plant line, namely three promising lines of irradiated black rice M7 (lines 13, 46, and 52) and lines without irradiation (control). The data were analyzed descriptively and qualitatively. The results showed that the three promising lines had better yield quality and performance than the control plants. The highest anthocyanin content and the best fat content were found in line 52, which were 75.04 ppm and 1.35%. Line 46 had the best protein content, vigor, and viability of 7.88%, 88%, and 67%, respectively. Line 13 had the highest amylose content of 14.92%. Line 52 was the best performing line (organoleptic staining and seed pericarp) compared to the other lines, namely 3.25 and 3.

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Na_(2)CO_(3)-responsive Photosynthetic and ROS Scavenging Mechanisms in Chloroplasts of Alkaligrass Revealed by Phosphoproteomics

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Genomics, Proteomics & Bioinformatics 2020年第3期18卷 271-288页

作者： Jinwei Suo Heng Zhang Qi Zhao Nan Zhang Yongxue Zhang Ying Li Baohua Song Juanjuan Yu Jianguo Cao Tai Wang Ji Luo Lihai Guo Jun Ma Xumin Zhang Yimin She Lianwei Peng Weimin Ma Siyi Guo Yuchen Miao Sixue Chen Zhi Qin Shaojun Dai Development Center of Plant Germplasm Resources College of Life and Environmental SciencesShanghai Normal UniversityShanghai 200234China Alkali Soil Natural Environmental Science Center Northeast Forestry UniversityKey Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil FieldMinistry of EducationHarbin 150040China State Key Laboratory of Subtropical Silviculture Zhejiang A&F UniversityHangzhou 311300China Institute of Botany Chinese Academy of SciencesBeijing 100093China AB Sciex Asia Pacific Application Support Center Shanghai 200233China Shanghai Center for Plant Stress Biology Chinese Academy of SciencesShanghai 201602China School of Life Sciences Fudan UniversityShanghai 200433China Institute of Plant Stress Biology State Key Laboratory of Cotton BiologyDepartment of BiologyHenan UniversityKaifeng 475004China Department of Biology Genetics InstitutePlant Molecular and Cellular Biology ProgramInterdisciplinary Center for Biotechnology ResearchUniversity of FloridaGainesvilleFL 32610USA

Alkali-salinity exerts severe osmotic,ionic,and high-p H stresses to *** understand the alkali-salinity responsive mechanisms underlying photosynthetic modulation and reactive oxygen species(ROS)homeostasis,physiological and diverse quantitative proteomics analyses of alkaligrass(Puccinellia tenuiflora)under Na_(2)CO_(3)stress were *** addition,Western blot,real-time PCR,and transgenic techniques were applied to validate the proteomic results and test the functions of the Na_(2)CO_(3)-responsive proteins.A total of 104 and 102 Na_(2)CO_(3)-responsive proteins were identified in leaves and chloroplasts,*** addition,84 Na_(2)CO_(3)-responsive phosphoproteins were identified,including 56 new phosphorylation sites in 56 phosphoproteins from chloroplasts,which are crucial for the regulation of photosynthesis,ion transport,signal transduction,and energy homeostasis.A full-length Pt FBA encoding an alkaligrass chloroplastic fructosebisphosphate aldolase(FBA)was overexpressed in wild-type cells of cyanobacterium Synechocystis *** PCC 6803,leading to enhanced Na_(2)CO_(3)*** these results indicate that thermal dissipation,state transition,cyclic electron transport,photorespiration,repair of photosystem(PS)Ⅱ,PSI activity,and ROS homeostasis were altered in response to Na_(2)CO_(3)stress,which help to improve our understanding of the Na_(2)CO_(3)-responsive mechanisms in halophytes.

关键词： Chloroplasts Na_(2)CO_(3)stress ROS scavenging Phosphoproteomics Puccinellia tenuiflora

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Unprecedented anti-inflammatory biscembranoids with 3-hydroperoxy-3-methylcyclohex-1-ene moiety isolated from aquaculture Sarcophyton trocheliophorum

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Aquaculture Reports 2023年 33卷

作者： Peng, Bo-Rong Nguyen, Ngoc Bao An Chen, Lo-Yun El-Shazly, Mohamed Hwang, Tsong-Long Su, Jui-Hsin Lai, Kuei-Hung Graduate Institute of Pharmacognosy College of Pharmacy Taipei Medical University Taipei 11031 Taiwan PhD Program in Clinical Drug Development of Herbal Medicine College of Pharmacy Taipei Medical University Taipei 11031 Taiwan Department of Pharmacognosy Faculty of Pharmacy Ain-Shams University Organization of African Unity Street Cairo Abassia 11566 Egypt Research Center for Chinese Herbal Medicine College of Human Ecology Chang Gung University of Science and Technology Taoyuan 33303 Taiwan Graduate Institute of Health Industry Technology College of Human Ecology Chang Gung University of Science and Technology Taoyuan 33303 Taiwan Department of Anaesthesiology Chang Gung Memorial Hospital Taoyuan 33305 Taiwan Graduate Institute of Natural Products College of Medicine Chang Gung University Taoyuan 33302 Taiwan Department of Chemical Engineering Ming Chi University of Technology New Taipei City 24301 Taiwan National Museum of Marine Biology & Aquarium Pingtung 94450 Taiwan Department of Marine Biotechnology and Resources National Sun Yat-sen University Kaohsiung 80424 Taiwan Traditional Herbal Medicine Research Center Taipei Medical University Hospital Taipei 11031 Taiwan

Recent advancements in the discovery of natural products have made significant progress in rapidly identifying known compounds from complex extracts using advanced spectroscopic technologies. Among these methods, one powerful approach called MS/MS molecular networking (MN) organizes chemical similarity based on MS/MS fragmentation data, serving as a valuable complement to traditional dereplication strategies. In our ongoing research focused on lead discovery from a sustainable medicinal source, an aquaculture soft coral Sarcophyton trocheliophorum, the MN profiling approach revealed the chemical diversity of biscembranoid analogs. By analyzing the MN pattern, biscembranoid derivatives with unique MS/MS fragments were prioritized for further investigation. Two novel biscembranoids, sarcotroxide A (1) and B (2), were successfully obtained through targeted isolation. These novel biscembranes contained an unprecedented 3-hydroperoxy-3-methylcyclohex-1-ene moiety compared with all biscembranoid analogs. The chemical structures of the two new biscembranoids were confirmed through spectroscopic analyses and in silico calculations. Their anti-inflammatory properties were assessed against the generation of superoxide anion (O₂^•−) and elastase release in activated human neutrophils. Sarcotroxides A (1) and B (2) exhibited anti-inflammatory activity by inhibiting O₂^•− accumulation (with 43.67% and 61.62% inhibition) and elastase release (with 38.21% and 61.62% inhibition) at 10 µM. These findings validated the potential of these biscembranoids from the cultured S. trocheliophorum as promising candidates for the future development of anti-inflammatory agents specifically designed to target neutrophils. © 2023 The Authors

关键词： Anti-inflammation Aquaculture soft coral Biscembranoid Neutrophil Sarcophyton trocheliophorum

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Deep learning-based air pollution analysis on carbon monoxide in Taiwan

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Ecological Informatics 2024年 80卷

作者： Cheng-Hong Yang Po-Hung Chen Chih-Hsien Wu Cheng-San Yang Li-Yeh Chuang Department of Information Management Tainan University of Technology Tainan Taiwan Department of Electronic Engineering National Kaohsiung University of Science and Technology Kaohsiung Taiwan Ph. D. Program in Biomedical Engineering Kaohsiung Medical University Kaohsiung Taiwan School of Dentistry Kaohsiung Medical University Kaohsiung Taiwan Drug Development and Value Creation Research Center Kaohsiung Medical University Kaohsiung Taiwan Division of Plastic and Reconstruction Surgery Department of Surgery Ditmanson Medical Foundation Chia-Yi Christian Hospital Chia-Yi Taiwan Department of Chemical Engineering and Institute of Biotechnology and Chemical Engineering I-Shou University Kaohsiung Taiwan

Global air pollution poses a threat to humanity. Specifically, CO directly affects cardiovascular and other organ tissues and leads to numerous chronic diseases and major public health problems. The effective implementation of a deep learning model for predicting variations in CO levels would enable the early formulation of policies for controlling air pollution. In this study, a seasonal gated recurrent unit (SGRU) model, which is a deep learning time-series prediction model, was developed to predict the levels of CO in Taiwan. Atmospheric CO measurements from 2005 to 2021 were collected from the Environmental Protection Administration of Taiwan and preprocessed using the Kalman filter to achieve accurate forecasting. The performance of the proposed SGRU model was compared with that of the autoregressive integrated moving average (ARIMA), seasonal ARIMA, exponential smoothing (ETS), Holt–Winters ETS, support vector regression, and seasonal long short-term memory models in terms of mean absolute percentage error (MAPE) and root mean square error. The SGRU model achieved the lowest MAPE value of 0.94, which demonstrated its superior performance. The construction of an accurate air pollution prediction model can assist government entities in formulating health and social care strategies and in planning future air pollution control measures.

关键词： Air pollution Carbon Monoxide (CO) Deep learning Seasonal gated recurrent unit (SGRU)

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Genetic variability of Phaius and Dendrobium orchids based on molecular markers

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IOP Conference Series: Earth and Environmental Science 2021年第1期637卷

作者： W L Yuhanna S Hartati Sugiyarto Marsusi Department of Biology Education Faculty of Teacher Training and Education Universitas PGRI Madiun Indonesia Department of Agrotechnology Faculty of Agriculture Sebelas Maret University Indonesia Center of Biotechnology and Biodiversity Research and Development Sebelas Maret University Indonesia Department of Biosciences Postgraduate Program Sebelas Maret University Indonesia

This study aims to assess the diversity and degree of grouping of several types of orchids in Indonesia. Laboratory work was carried out at the Laboratory of the Center for Horticulture and Tropical Studies IPB by analyzing the variability of orchids using RAPD. Plant material is taken from the orchid collection at the Bogor Botanical Gardens. The relationship between 9 orchids was analyzed using binary phenotype data and DNA band patterns as assessed by amplification of 2 random RAPD primers. The cluster analysis was carried out under the NTSYSpc version 2.02 program and the UPGMA SimQual function. The results showed genetic diversity of the genus Phaius and Dendrobium. Based on primers OPA7 and OPA9 with a similarity coefficient of 0.4 divided into 4 groups. Group I consisted of Phaius tankervilliae, Phaius montanus and Phaius callosus. Group II consisted of Dendrobium mirbelianum, Dendrobium lamellatum, Dendrobium liniale and Dendrobium biggibum. Group III consists of Dendrobium anosmum. Group IV consisted of Phaius ambionensis. The conclusion of this study is that there are 4 groups from the genus Phaius and Dendrobium based on the genetic marker RAPD.

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Interactive Effects of a Combination of the HDAC3 and HDAC9 Genes with Diabetes Mellitus on the Risk of Ischemic Stroke

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Thrombosis and Haemostasis 2020年第3期121卷 396-404页

作者： Hung-Yi Chiou Chyi-Huey Bai Li-Ming Lien Chaur-Jong Hu Jiann-Shing Jeng Sung-Chun Tang Huey-Juan Lin Yi-Chen Hsieh School of Public Health College of Public Health Taipei Medical University Taipei Taiwan Master Program in Applied Molecular Epidemiology College of Public Health Taipei Medical University Taipei Taiwan School of Medicine College of Medicine Taipei Medical University Taipei Taiwan Department of Neurology Shin Kong Wu Ho-Su Memorial Hospital Taipei Taiwan Stroke Center Department of Neurology Shuang Ho Hospital Taipei Medical University New Taipei City Taiwan Stroke Center and Department of Neurology National Taiwan University Hospital Taipei Taiwan Department of Emergency Medicine Chi-Mei Medical Center Tainan Taiwan Department of Biotechnology Southern Taiwan University of Science and Technology Tainan Taiwan PhD Program of Neural Regenerative Medicine College of Medical Science and Technology Taipei Medical University Taipei Taiwan PhD Program in Biotechnology Research and Development College of Pharmacy Taipei Medical University Taipei Taiwan

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Existing diversity profile for kernel characteristics of maize germplasm in IAARD-ICABIOGRAD gene bank

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AIP Conference Proceedings 2022年第1期2462卷

作者： Andari Risliawati Sobir Trikoesoemaningtyas Willy B. Suwarno Puji Lestari 1Plant Breeding and Biotechnology Graduate Program Graduate School of IPB University Jln. Meranti Dramaga Campus Bogor 16680 West Java Indonesia 2Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development IAARD Jln. Tentara Pelajar No. 3A Bogor 16111 West Java Indonesia 3Department of Agronomy and Horticulture Faculty of Agriculture IPB University Jln. Meranti Dramaga Campus Bogor 16680 West Java Indonesia 4Indonesian Center for Rice Research IAARD Jln. Raya No. 9 Sukamandi Subang 41256 West Java Indonesia

Maize (Zea mays L.), a model genetic organism with immense genetic diversity, is one of the major commodities in Indonesia. The genetic diversity of this crop is an important resource for their genetic improvement through breeding activities, thus properly conserving and managing the germplasm is necessary. This valuable maize ex situ germplasm is being prioritized to be conserved in the Indonesian Agency for Agricultural research and development-Indonesian Center for Agricultural and biotechnology and Genetic Resources research and development Gene Bank (IAARD-ICABIOGRAD Gene Bank) for decades. However, the diversity information of the whole germplasm has been not reported due to the large collection which is challenged for the management. The research aimed at investigating the genetic diversity from whole maize germplasm conserved in IAARD-ICABIOGRAD Gene Bank, specifically on kernel characteristics as a major trait contributes to maize diversity. A total of 1,279 accessions were characterized for their kernel characteristics using a modified maize descriptor and followed by a series of diversity analyses. The results showed a moderate-high genetic diversity existed among the accessions. Two out of nine kernel morphological characters revealed the major contribution to genetic diversity among the accessions. These characters successfully differentiated all accessions into ten clusters, of which specific traits were observed as cluster identity. This preliminary investigation of kernel diversity within maize germplasm may become valuable information for gene bank managers to determine the appropriate decision for germplasm management and inform users about the existing diversity profile of the germplasm.

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Briavioid D, a rare 7β,8β-epoxybriarane from Briareum violaceum

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Tetrahedron Letters 2023年 118卷

作者： Thanh Hao Huynh Chou-Yuan Ko Hsin-Tzu Liu Yen-Po Chen Mingzi M. Zhang Lun Kelvin Tsou Zhi-Hong Wen Su-Ying Chien Ping-Jyun Sung Department of Marine Biotechnology and Resources National Sun Yat-sen University Kaohsiung 804201 Taiwan National Museum of Marine Biology and Aquarium Pingtung 944401 Taiwan Division of Gastroenterology and Hepatology Department of Internal Medicine Kaohsiung Armed Forces General Hospital Kaohsiung 802301 Taiwan Institute of Medical Science and Technology National Sun Yat-sen University Kaohsiung 804201 Taiwan Department of Medical Research Hualien Tzu Chi Hospital Buddhist Tzu Chi Medical Foundation Hualien 970473 Taiwan Department of Obstetrics and Gynecology Kaohsiung Armed Forces General Hospital Kaohsiung 802301 Taiwan Institute of Molecular and Genomic Medicine National Health Research Institutes Zhunan Miaoli 350401 Taiwan Institute of Biotechnology and Pharmaceutical Research National Health Research Institutes Zhunan Miaoli 350401 Taiwan Institute of BioPharmaceutical Sciences National Sun Yat-sen University Kaohsiung 804201 Taiwan Instrumentation Center National Taiwan University Taipei 106319 Taiwan Chinese Medicine Research and Development Center China Medical University Hospital Taichung 404394 Taiwan Graduate Institute of Natural Products Kaohsiung Medical University Kaohsiung 807378 Taiwan Ph.D. Program in Pharmaceutical Biotechnology Fu Jen Catholic University New Taipei City 242062 Taiwan

A rare 7β,8β-epoxybriarane, briavioid D ( 1 ), has been isolated from a cultured octocoral Briareum violaceum . The structure of 1 was deduced by extensive 2D NMR studies. Single-crystal X-ray diffraction analysis was performed to identify the structure of 1 and this compound was found to reduce the release of iNOS to 85.77 %, at a concentration of 10 μM.

关键词： Briareum violaceum Briavioid Briarane X-ray iNOS

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Cover Image, Volume 236, Number 9, September 2021

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Journal of Cellular Physiology 2021年第9期236卷

作者： Fatemeh Ghaemimanesh Majid Mehravar Saeideh Milani Ensieh M. Poursani Kioomars Saliminejad Monoclonal Antibody Research Center Avicenna Research Institute ACECR Tehran Iran Department of Anatomy and Developmental Biology Development and Stem Cells Program Biomedicine Discovery Institute Monash University Melbourne Australia Blood Transfusion Research Center High Institute for Research and Education in Transfusion Medicine Tehran Iran Hematology Oncology and Stem Cell Transplantation Research Center Tehran University of Medical Sciences Tehran Iran Reproductive Biotechnology Research Center Avicenna Research Institute ACECR Tehran Iran

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