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Publisher Correction: Sex-dimorphic genetic effects and novel loci for fasting glucose and insulin variability

Nature communications 2021年第1期12卷 995页

作者： Vasiliki Lagou Reedik Mägi Jouke- Jan Hottenga Harald Grallert John R B Perry Nabila Bouatia-Naji Letizia Marullo Denis Rybin Rick Jansen Josine L Min Antigone S Dimas Anna Ulrich Liudmila Zudina Jesper R Gådin Longda Jiang Alessia Faggian Amélie Bonnefond Joao Fadista Maria G Stathopoulou Aaron Isaacs Sara M Willems Pau Navarro Toshiko Tanaka Anne U Jackson May E Montasser Jeff R O'Connell Lawrence F Bielak Rebecca J Webster Richa Saxena Jeanette M Stafford Beate St Pourcain Nicholas J Timpson Perttu Salo So-Youn Shin Najaf Amin Albert V Smith Guo Li Niek Verweij Anuj Goel Ian Ford Paul C D Johnson Toby Johnson Karen Kapur Gudmar Thorleifsson Rona J Strawbridge Laura J Rasmussen-Torvik Tõnu Esko Evelin Mihailov Tove Fall Ross M Fraser Anubha Mahajan Stavroula Kanoni Vilmantas Giedraitis Marcus E Kleber Günther Silbernagel Julia Meyer Martina Müller-Nurasyid Andrea Ganna Antti-Pekka Sarin Loic Yengo Dmitry Shungin Jian'an Luan Momoko Horikoshi Ping An Serena Sanna Yvonne Boettcher N William Rayner Ilja M Nolte Tatijana Zemunik Erik van Iperen Peter Kovacs Nicholas D Hastie Sarah H Wild Stela McLachlan Susan Campbell Ozren Polasek Olga Carlson Josephine Egan Wieland Kiess Gonneke Willemsen Johanna Kuusisto Markku Laakso Maria Dimitriou Andrew A Hicks Rainer Rauramaa Stefania Bandinelli Barbara Thorand Yongmei Liu Iva Miljkovic Lars Lind Alex Doney Markus Perola Aroon Hingorani Mika Kivimaki Meena Kumari Amanda J Bennett Christopher J Groves Christian Herder Heikki A Koistinen Leena Kinnunen Ulf de Faire Stephan J L Bakker Matti Uusitupa Colin N A Palmer J Wouter Jukema Naveed Sattar Anneli Pouta Harold Snieder Eric Boerwinkle James S Pankow Patrik K Magnusson Ulrika Krus Chiara Scapoli Eco J C N de Geus Matthias Blüher Bruce H R Wolffenbuttel Michael A Province Goncalo R Abecasis James B Meigs G Kees Hovingh Jaana Lindström James F Wilson Alan F Wright George V Dedoussis Stefan R Bornstein Peter E H Schwarz Anke Tönjes Bernhard R Winkelmann Bernhard O Boehm Winfried März Andres Metspalu Jackie F Price Panos Delouk Wellcome Centre for Human Genetics University of Oxford Oxford UK. Department of Microbiology and Immunology Laboratory of Adaptive Immunity KU Leuven Leuven Belgium. VIB-KU Leuven Center for Brain and Disease Research Leuven Belgium. Estonian Genome Centre Institute of Genomics University of Tartu Tartu Estonia. Department of Biological Psychology Vrije Universiteit Amsterdam The Netherlands. Amsterdam Public Health Research Institute VU University medical center Amsterdam The Netherlands. Research Unit of Molecular Epidemiology Institute of Epidemiology Helmholtz Zentrum München Research Center for Environmental Health Neuherberg Germany. German Center for Diabetes Research (DZD München-Neuherberg Germany. MRC Epidemiology Unit University of Cambridge School of Clinical Medicine Cambridge UK. University of Lille Nord de France Lille France. CNRS UMR8199 Institut Pasteur de Lille Lille France. INSERM U970 Paris Cardiovascular Research Center PARCC 75006 Paris France. Department of Life Sciences and Biotechnology University of Ferrara Ferrara Italy. Boston University Data Coordinating Center Boston MA USA. Department of Psychiatry VU University Medical Center Amsterdam The Netherlands. MRC Integrative Epidemiology Unit University of Bristol Bristol UK. Bristol Medical School University of Bristol Bristol UK. Institute for Bioinnovation Biomedical Sciences Research Center Al. Fleming Vari Greece. Department of Medicine Imperial College London London UK. Cardiovascular Medicine Unit Center for Molecular Medicine Department of Medicine Karolinska Institutet Stockholm Karolinska University Hospital Solna Sweden. Institute for Molecular Bioscience The University of Queensland Brisbane QLD 4072 Australia. Department of Epidemiology Research Statens Serum Institut Copenhagen Denmark. Université de Lorraine IGE-PCV F-54000 Nancy France. Genetic Epidemiology Unit Department of Epidemiology Erasmus Medical Center Rotterdam the Netherlands. CAR

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Transferability of European-derived Alzheimer’s disease polygenic risk scores across multiancestry populations

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Nature Genetics 2025年 1-13页

作者： Aude Nicolas Benjamin Grenier-Boley Céline Bellenguez Philippe Amouyel Jean-Charles Lambert Richard Sherva Rui Zhang Mark Logue Lindsay Farrer Yoontae Kim Kun Ho Lee Jungsoo Gim Masataka Kikuchi Jigyasha Timsina Carlos Cruchaga Itziar de Rojas Mercè Boada Pablo García-González Raquel Puerta Agustín Ruiz Oriol Dols-Icardo Fermin Moreno Jordi Pérez-Tur María J. Bullido Pablo Mir Luis M. Real Jose María García-Alberca Eloy Rodriguez-Rodriguez Sonia Moreno-Grau Pablo Garcia-Gonzalez Carla Abdelnour Daniel Alcolea Montserrat Alegret Rafael Blesa Dolores Buiza-Rueda Miguel Calero Laura Cervera-Carles Jordi Clarimon Ana Espinosa Juan Fortea Ana Frank-García Jose Maria García-Alberca Isabel Hernández Carmen Lage Alberto Lleó Adolfo López-de Munain Marta Marquié Angel Martín Montes Miguel Medina Adelina Orellana Gemma Ortega Alberto Rábano Maitée Rosende-Roca Ángela Sanabria Pascual Sánchez-Juan Lluís Tárraga Sergi Valero Miren Zulaica María Carolina Dalmasso Xiaopu Zhou Han Cao Nancy Y. Ip Amy K. Y. Fu Fanny C. F. Ip Yann Le Guen Michael D. Greicius Carlos E. Arboleda-Bustos Jenny Ortega-Rojas Ana Gabriela Concha Mera Maria F. Mahecha Rodrigo Pardo Gonzalo Arboleda Humberto Arboleda Maria Aparecida Camargos Bicalho Edgar Nunes de Moraes Marco Túlio Gualberto Cintra Luiz Armando de Marco Bernardo de Mattos Viana Marco Aurélio Romano Silva Maëlenn Guerchet Pierre-Marie Preux Pascal Mbelesso Sven van der Lee Yolande A. L. Pijnenburg Henne Holstege Philip Scheltens Marc Hulsman Niccolo Tesí Wiesje van der Flier Danielle Posthuma Monica Goss Claudia L. Satizabal Bernard Fongang Sudha Seshadri Atahualpa Castillo Julie Williams Rebecca Sims Fahri Küçükali Jasper Van Dongen Kristel Sleegers Qiong Yang Oliver Peters Josef Priller Anja Schneider Frank Jessen Martin Dichgans Dan Rujescu Norbert Scherbaum Jürgen Deckert Steffi Riedel-Heller Lucrezia Hausner Laura Molina-Porcel Emrah Düzel Timo Grimmer Jens Wiltfang Jonathan Vogelgsang Stefanie Heilmann-Heimbach Per Hoffmann Markus M. Möthen Susanne Moebus Thomas Tegos Tsolaki Anthou U1167-RID-AGE facteurs de risque et déterminants moléculaires des maladies liées au vieillissement Université Lille Inserm CHU Lille Institut Pasteur de Lille Lille France Institut du Cerveau - Paris Brain Institute - ICM Sorbonne Université CNRS APHP Hôpital de La Pitié Salpêtrière Inserm Paris France Behavioral Sciences Division National Center for PTSD VA Boston Healthcare System Boston MA USA Department of Medicine (Biomedical Genetics) Boston University Boston MA USA Department of Biostatistics Boston University School of Public Health Boston MA USA Department of Psychiatry Boston University Chobanian and Avedisian School of Medicine Boston MA USA Biomedical Genetics/Department of Medicine Boston University Chobanian and Avedisian School of Medicine Boston MA USA Department of Epidemiology Boston University Boston MA USA Department of Neurology Boston University Boston MA USA Department of Ophthalmology Boston University Boston MA USA BK FOUR Department of Integrative Biological Sciences Chosun University Gwangju Republic of Korea Department of Biomedical Science Chosun University Gwangju Republic of Korea Gwangju Alzheimer’s and Related Dementia (GARD) Cohort Research Center Chosun University Gwangju Republic of Korea Graduate School of Frontier Science University of Tokyo Tokyo Japan Department of Psychiatry Washington University School of Medicine St. Louis MO USA NeuroGenomics and Informatics Center Washington University School of Medicine St. Louis MO USA ACE Alzheimer Center Barcelona Universitat Internacional de Catalunya Barcelona Spain CIBERNED Network Center for Biomedical Research in Neurodegenerative Diseases National Institute of Health Carlos III Madrid Spain Glenn Biggs Institute for Alzheimer’s and Neurodegenerative Diseases University of Texas Health Sciences Center San Antonio TX USA Genetics of Neurodegenerative Diseases Unit IIB Sant Pau Barcelona Spain Department of Neurology Hospital Universitario Donostia S

A polygenic score (PGS) for Alzheimer’s disease (AD) was derived recently from data on genome-wide significant loci in European ancestry populations. We applied this PGS to populations in 17 European countries and observed a consistent association with the AD risk, age at onset and cerebrospinal fluid levels of AD biomarkers, independently of apolipoprotein E locus (APOE). This PGS was also associated with the AD risk in many other populations of diverse ancestries. A cross-ancestry polygenic risk score improved the association with the AD risk in most of the multiancestry populations tested when theAPOEregion was included. Finally, we found that the PGS/polygenic risk score captured AD-specific information because the association weakened as the diagnosis was broadened. In conclusion, a simple PGS captures the AD-specific genetic information that is common to populations of different ancestries, although studies of more diverse populations are still needed to better characterize the genetics of AD.

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Author Correction: Identification of genes required for eye development by high-throughput screening of mouse knockouts

Communications Biology

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Communications Biology 2019年第1期2卷 1-2页

作者： Bret A. Moore Brian C. Leonard Lionel Sebbag Sydney G. Edwards Ann Cooper Denise M. Imai Ewan Straiton Luis Santos Christopher Reilly Stephen M. Griffey Lynette Bower David Clary Jeremy Mason Michel J. Roux Hamid Meziane Yann Herault Colin McKerlie Ann M. Flenniken Lauryl M. J. Nutter Zorana Berberovic Celeste Owen Susan Newbigging Hibret Adissu Mohammed Eskandarian Chih-Wei Hsu Sowmya Kalaga Uchechukwu Udensi Chinwe Asomugha Ritu Bohat Juan J. Gallegos John R. Seavitt Jason D. Heaney Arthur L. Beaudet Mary E. Dickinson Monica J. Justice Vivek Philip Vivek Kumar Karen L. Svenson Robert E. Braun Sara Wells Heather Cater Michelle Stewart Sharon Clementson-Mobbs Russell Joynson Xiang Gao Tomohiro Suzuki Shigeharu Wakana Damian Smedley J. K. Seong Glauco Tocchini-Valentini Mark Moore Colin Fletcher Natasha Karp Ramiro Ramirez-Solis Jacqueline K. White Martin Hrabe de Angelis Wolfgang Wurst Sara M. Thomasy Paul Flicek Helen Parkinson Steve D. M. Brown Terrence F. Meehan Patsy M. Nishina Stephen A. Murray Mark P. Krebs Ann-Marie Mallon K. C. Kent Lloyd Christopher J. Murphy Ala Moshiri William R. Pritchard Veterinary Medical Teaching Hospital School of Veterinary Medicine University of California-Davis Davis USA Department of Surgical and Radiological Sciences School of Veterinary Medicine University of California-Davis Davis USA Comparative Pathology Laboratory School of Veterinary Medicine University of California-Davis Davis USA Medical Research Council Harwell Institute (Mammalian Genetis Unit and Mary Lyon Center) Harwell Oxfordshire UK Mouse Biology Program and Department of Surgery School of Medicine University of California-Davis Davis USA European Molecular Biology Laboratory European Bioinformatics Institute Wellcome Genome Campus Hinxton Cambridge UK Institut de Génétique et de Biologie Moléculaire et Cellulaire Université de Strasbourg Illkirch France Centre National de la Recherche Scientifique Illkirch France Institut National de la Santé et de la Recherche Médicale Illkirch France Université de Strasbourg Illkirch France CELPHEDIA PHENOMIN Institut Clinique de la Souris (ICS) CNRS INSERM University of Strasbourg Illkirch-Graffenstaden France The Centre for Phenogenomics Toronto Canada The Hospital for Sick Children Toronto Canada Lunenfeld-Tanenbaum Research Institute Mount Sinai Hospital Toronto Canada Department of Molecular Physiology and Biophysics Baylor College of Medicine Houston USA Department of Molecular and Human Genetics Baylor College of Medicine Houston USA The Jackson Laboratory Bar Harbor USA SKL of Pharmaceutical Biotechnology and Model Animal Research Center Collaborative Innovation Center for Genetics and Development Nanjing Biomedical Research Institute Nanjing University Nanjing China RIKEN BioResource Center Tsukuba Japan Clinical Pharmacology Charterhouse Square Barts and the London School of Medicine and Dentistry Queen Mary University of London London UK Korea Mouse Phenotyping Consortium (KMPC) and BK21 Program for Veterinary Science Research Institute for Veterinary Science College of

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Molecular analysis of Toxoplasma gondii Surface Antigen 1 (SAG1) gene cloned from Toxoplasma gondii DNA isolated from Javanese acute toxoplasmosis

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Journal of Physics: Conference Series 2018年第1期1022卷

作者： Sri Haryati Afiono Agung Prasetyo Yulia Sari Ruben Dharmawan A-IGIC (A-Infection Genomics Immunology & Cancer) Research Group Universitas Sebelas Maret Jl. Ir. Sutami 36A Surakarta 57126 Indonesia. Department of Parasitology Faculty of Medicine Universitas Sebelas Maret Jl. Ir. Sutami 36A Surakarta 57126 Indonesia. Center of Biotechnology and Biodiversity Research and Development Universitas Sebelas Maret Jl. Ir. Sutami 36A Surakarta 57126 Indonesia. Division of Virology Department of Microbiology Faculty of Medicine Universitas Sebelas Maret Jl. Ir. Sutami 36A Surakarta 57126 Indonesia. Doctoral Program of Medical Sciences Universitas Sebelas Maret Jl. Ir. Sutami 36A Surakarta 57126 Indonesia.

Toxoplasma gondii Surface Antigen 1 (SAG1) is often used as a diagnostic tool due to its immunodominant-specific as antigen. However, data of the Toxoplasma gondii SAG1 protein from Indonesian isolate is limited. To study the protein, genomic DNA was isolated from a Javanese acute toxoplasmosis blood samples patient. A complete coding sequence of Toxoplasma gondii SAG1 was cloned and inserted into an Escherichia coli expression plasmid and sequenced. The sequencing results were subjected to bioinformatics analysis. The Toxoplasma gondii SAG1 complete coding sequences were successfully cloned. Physicochemical analysis revealed the 336 aa of SAG1 had 34.7 kDa of weight. The isoelectric point and aliphatic index were 8.4 and 78.4, respectively. The N-terminal methionine half-life in Escherichia coli was more than 10 hours. The antigenicity, secondary structure, and identification of the HLA binding motifs also had been discussed. The results of this study would contribute information about Toxoplasma gondii SAG1 and benefits for further works willing to develop diagnostic and therapeutic strategies against the parasite.

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Human T-lymphotropic Virus-1/2 detected in drug abused men who have sex with men infected with HIV in Surakarta, Indonesia

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Journal of Physics: Conference Series 2018年第1期1022卷

作者： Afiono Agung Prasetyo Yulia Sari A-IGIC (A-Infection Genomics Immunology & Cancer) Research Group Universitas Sebelas Maret Jl. Ir. Sutami 36A Surakarta 57126 Indonesia. Center of Biotechnology and Biodiversity Research and Development Universitas Sebelas Maret Jl. Ir. Sutami 36A Surakarta 57126 Indonesia. Division of Virology Department of Microbiology Faculty of Medicine Universitas Sebelas Maret Jl. Ir. Sutami 36A Surakarta 57126 Indonesia. Department of Parasitology Faculty of Medicine Universitas Sebelas Maret Jl. Ir. Sutami 36A Surakarta 57126 Indonesia. Doctoral Program of Medical Sciences Faculty of Medicine Universitas Sebelas Maret Jl. Ir. Sutami 36A Surakarta Indonesia.

Human T-lymphotropic virus types 1 and 2 (HTLV-1/2) share similar routes of transmission with human immunodeficiency virus (HIV), and the HTLV-1/2 co-infection may affect the clinical course of HIV infection. The HIV/HTLV-1/2 co-infection risk higher if the patient performing the high-risk activities. This study evaluated the presentation of HTLV-1 and 2 in HIV-infected men who have sex with men with drug abused history in Surakarta Indonesia. Blood samples collected from HIV-infected men who have sex with men with drug abused history in Surakarta were tested using HTLV-1/2 enzyme-linked immunosorbent assays and confirmed by RT-PCR nested addressed the part of HTLV-1 LTR and HTLV-2 LTR region, respectively. The specificity of the molecular assays was confirmed by sequencing the amplicons. The anti HTLV-1/2 positive rate was 17.4% (8/46). All positive serological samples were confirmed by nested RT-PCR. Of these, three was HTLV-1 positive and five was HTLV-2 positive. Molecular analysis of positive PCR products revealed that all HTLV-1 isolates had a close relationship with HTLV-1 isolated in Japan while all HTLV-2 isolates with that of isolated in the USA. HTLV-1 and HTLV-2 were detected in drug abused men who have sex with men infected with HIV in Surakarta.

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Malachite Green and Leuco-Malachite Green Detection in Fish Using Modified Enzyme Biosensor

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Procedia Chemistry 2016年 20卷 85-89页

作者： A.P. Nurul Hidayah S. Faridah M.S. Nur Azura A.R. Gayah M. Othman A.B. Fatimah Agri-Nanotechnology Program Biotechnology and Nanotechnology Research Centre Malaysian Agricultural Research and Development Institute (MARDI) Persiaran MARDI-UPM 43400 Serdang Selangor D.E. Malaysia Fishery Research Institute Malaysia FRI Batu Maung Bayan Lepas 11960 Batu Maung Pulau Pinang Malaysia Department of Food Science Faculty of Food Science and Technology Universiti Putra Malaysia 43400 UPM Serdang Selangor D.E. Malaysia

A modified enzyme-based biosensor was developed for amperometric measurement of total Malachite Green (sum of MG and LMG) in tilapia fish. The 4 UmL -1 butyrylcholinesterase enzyme (BuChE) was used and immobilized onto the surface of carbon paste electrode during the electro-polymerization of polypyrrole (PPy). It is a simple, rapid and sensitive technique used for determination of total MG using 0.1M phosphate buffer pH 8.0. In this study, butyrylthiocholine iodide (0.3 Mm BTCi) was used as a substrate which produced the thiocholine due to the enzymatic hydrolysis. Then, it was oxidized at 0.4V against silver-silver chloride electrode for 100 s. The modified enzyme biosensor provided a high sensitivity and linear concentration range from 0.25 to 10 ppb with a limit of detection (LOD) at 0.25 ppb. The results showed the linear standard curve of total MG standard solution (based on the current output in microampere) with the equation of y = - 0.9113x + 10.84, R 2 = 0.9445.

关键词： Malachite Green Leuco-Malachite Green Biosensor Butyrylcholinesterase Polypyrrole

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Comparison of Pretreatment Methods on Vetiver Leaves for Efficient Processes of Simultaneous Saccharification and Fermentation by Neurospora sp.

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Journal of Physics: Conference Series 2017年第1期877卷

作者： E Restiawaty A Dewi Study Program of Bioenergy and Chemurgy Engineering Research Group of Chemical Engineering Process Design and Development Faculty of Industrial Technology Institut Teknologi Bandung Jln. Let. Jen. Purn. Dr. (HC) Mashudi No. 1 Sumedang West Java 45363 Indonesia Biosciences and Biotechnology Research Centre Institut Teknologi Bandung Jln. Ganesha 10 Bandung 40132 Indonesia

Lignocellulosic biomass is a potential raw material for bioethanol production. Neurospora sp. can be used to convert lignocellulosic biomass into bioethanol because of its ability to perform simultaneous saccharification and fermentation. However, lignin content, degree of polymerization, and crystallinity of cellulose contained in lignocellulosic biomass can inhibit cellulosic-biomass digestion by Neurospora sp, so that a suitable pretreatment method of lignocellulosic biomass is needed. The focus of this research was to investigate the suitable pretreatment method for vetiver leaves (Vetiveria zizanioides L. Nash) used as a raw material producing bioethanol in the process of simultaneous saccharification and fermentation (SSF) by Neurospora sp.. Vetiver plants obtained from Garut are deliberately cultivated to produce essential oils extracted from the roots of this plant. Since the vetiver leaves do not contain oil, some of harvested leaves are usually used for crafts and cattle feed, and the rest are burned. This study intended to look at other potential of vetiver leaves as a source of renewable energy. Pretreatments of the vetiver leaves were conducted using hot water, dilute acid, alkaline & dilute acid, and alkaline peroxide, in which each method was accompanied by thermal treatment. The results showed that the alkaline peroxide treatment is a suitable for vetiver leaves as indicated by the increase of cellulose content up to 65.1%, while the contents of hot water soluble, hemicellulose, lignin, and ash are 8.7%, 18.3%, 6.8%, and 1.1%, respectively. Using this pretreatment method, the vetiver leaves can be converted into bioethanol by SSF process using Neurospora sp. with a concentration of bioethanol of 6.7 g/L operated at room temperature.

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Corrigendum: Rapid and visual Chlamydia trachomatis detection using loop-mediated isothermal amplification and hydroxynaphthol blue

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Letters in applied microbiology 2017年第4期65卷 343页

作者： I Choopara N Arunrut W Kiatpathomchai D Dean N Somboonna Program in Biotechnology Faculty of Science Chulalongkorn University Bangkok Thailand. Bioengineering and Sensing Technology Laboratory National Center for Genetic Engineering and Biotechnology Khlong Nueng Khlong Luang Pathum Thani Thailand. Center for Immunobiology and Vaccine Development UCSF Benioff Children's Hospital Oakland Research Institute Oakland CA USA. Department of Medicine University of California San Francisco CA USA. Joint Graduate Program in Bioengineering University of California Berkeley CA USA. Department of Microbiology Faculty of Science Chulalongkorn University Bangkok Thailand.

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Correction to: Culture of mesenchymal stem cells derived from equine synovial membrane in alginate hydrogel microcapsules

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Stem cell research & therapy 2018年第1期9卷 259页

作者： Vitor Hugo Santos João Pedro Hübbe Pfeifer Jaqueline Brandão de Souza Betsabéia Heloisa Gentilha Milani Rogério Antonio de Oliveira Marjorie Golim Assis Elenice Deffune Andrei Moroz Ana Liz Garcia Alves Department of Veterinary Surgery and Anesthesiology University of Veterinary Medicine and Animal Science UNESP District of Rubião Júnior s / n Botucatu São Paulo Brazil. Departament of Statistics Institute of Biosciences UNESP District of Rubião Júnior s / n Botucatu SP Brazil. Departament of Graduate Program in Research and Development Medical Biotechnology UNESP Blood Centre Division District of Rubião Júnior s / n Botucatu SP Brazil. Departament of Urology Blood Centre Division - Laboratory of Cellular Engineering University of Medicine UNESP District of Rubião Junior s / n Botucatu SP Brazil. Departament of Bioprocesses and Biotechnology FCFAR - UNESP Rodovia Araraquara Jaú KM 01 São Paulo Brazil. Department of Veterinary Surgery and Anesthesiology University of Veterinary Medicine and Animal Science UNESP District of Rubião Júnior s / n Botucatu São Paulo Brazil. anaalves@fmvz.unesp.br.

The original article [1] contained a minor error regarding the mean diameter of the alginate microcapsules described in relation to Fig. 4 in the Results section. The microcapsules had an actual mean diameter of ...

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Potential of Fermentable Sugar Production from Napier cv. Pakchong 1 Grass Residue as a Substrate to Produce Bioethanol

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Energy Procedia 2016年 89卷 428-436页

作者： Bunthita Pensri Pruk Aggarangsi Thanongsak Chaiyaso Nopakarn Chandet Biotechnology Program Graduate School Chiang Mai University Suthep District Ampher Muang Chiang Mai 50200 Thailand Energy Research and Development Institute Nakornping Chiang Mai University Suthep District Ampher Muang Chiang Mai 50200 Thailand Multidisciplinary Science Research Centre Faculty of Science Chiang Mai University Suthep District Ampher Muang Chiang Mai 50200 Thailand Division of Biotechnology Faculty of Agro-Industry Chiang Mai University Suthep District Ampher Muang Chiang Mai 50200 Thailand Department of Chemistry Faculty of Science Chiang Mai University Suthep District Ampher Muang Chiang Mai 50200 Thailand

Bioethanol is one of the most significant renewable fuels. The major sources of bioethanol production are food crops such as corn, sugarcane, rice, wheat and sugar beet. However, utilization of food crops to produce bioethanol could affect the food sources and disrupt the food to population ratio. To overcome these issues, the utilization of lignocellulosic materials such as wheat straw, grass and crop residues to produce bioethanol has been developed for second-generation fuel, since those resources are abundant, cheap and renewable. Napier Pakchong 1 grass (NPG) residue is a lignocellulosic waste obtained from the process of biogas production that can be used as an alternative material for bioethanol production. This research aims to study on the potential of fermentable sugar production from NPG residue. The materials were pretreated with different concentrations of sodium hydroxide (NaOH), followed by enzymatic hydrolysis for saccharification. The results suggested that pretreatment with 3.0% (w/v) NaOH solution at 121̊C for 60 minutes provided the highest lignin removal of 86.1% (w/w) and enriched cellulose fraction from 36.4 to 75.6% (w/w). The enzymatic hydrolysis was conducted by varying enzyme loading volume and total solid contents (TS) at pH 4.8, 50̊C for 72 h. The hydrolysis with enzyme loading volume of 2.0 ml/g of substrate and 10% (w/v) of TS were optimal for saccharification giving the reducing sugar yield of 768 mg/g of pretreated biomass or equal to 64 g/L and glucose yield of 522 mg/g of pretreated biomass or equal to 43 g/L. The reducing sugar will be used as a starting material for yeast to produce bioethanol.

关键词： Fermentable sugar production Napier cv. Pakchong 1 grass Bioethanol

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