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Molecular architecture of mammalian pyruvate dehydrogenase complex

Protein & Cell 2025年第1期16卷 72-78页

作者： Maofei Chen Yutong Song Sensen Zhang Yitang Zhang Xudong Chen Minghui Zhang Meng Han Xin Gao Sai Li Maojun Yang Tsinghua-Peking Center for Life Sciences Beijing Frontier Research Center for Biological StructureSchool of Life SciencesTsinghua UniversityBeijing 100084China Ministry of Education Key Laboratory of Protein Science Tsinghua UniversityBeijing 100084China State Key Laboratory of Membrane Biology Tsinghua UniversityBeijing 100084China Department of Biochemistry and Molecular Biology School of Basic Medical SciencesShenzhen University Health Science CenterShenzhen 518060China Protein Research Technology Center Protein Chemistry and Omics PlatformSchool of Life SciencesTsinghua UniversityBeijing 100084China Computer Science Program ComputerElectrical and Mathematical Sciences and Engineering DivisionKing Abdullah University of Science and Technology(KAUST)Thuwal 23955-6900Kingdom of Saudi Arabia Computational Bioscience Research Center King Abdullah University of Science and TechnologyThuwal 23955-6900Kingdom of Saudi Arabia Cryo-EM Facility Center Southern University of Science and TechnologyShenzhen 518055China Beijing Life Science Academy Beijing 102209China

Dear Editor,Pyruvate dehydrogenase complex(PDHc) is a large multienzyme assembly(Mr = 4–10 million Daltons) consisting of three essential components: pyruvate dehydrogenase(E1p), dihydrolipoyl transacetylase(E2p), and dihydrolipoyl dehydrogenase(E3). These three enzymes perform distinct functions sequentially to catalyze the oxidative decarboxylation of pyruvate with formation of nicotinamide adenine dinucleotide(NADH) and acetyl-coenzyme A(Patel and Roche, 1990).

关键词： sequentially oxidative Dalton

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Comparative Analysis of SAAS Model and NPC Integration for Enhancing VR Shopping Experiences

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Applied sciences (Switzerland) 2024年第15期14卷 6573页

作者： Doungtap, Surasachai Wang, Jenq-Haur Phanichraksaphong, Varinya International Graduate Program of Electrical Engineering and Computer Science National Taipei University of Technology Taipei10608 Taiwan Department of Computer Science and Information Engineering National Taipei University of Technology Taipei10608 Taiwan

This article examines the incorporation of the Shopping Assistance Automatic Suggestion (SAAS) model into Virtual Reality (VR) environments in order to improve the online shopping experience. The SAAS model employs sophisticated deep learning methods to offer customized product recommendations, which are conveyed by non-player characters (NPCs) via voice-based interactions. Our goal is to develop an interactive shopping experience that replicates real-life interactions by integrating AI-powered recommendations with immersive VR technology. We gather and standardize data from several open commerce databases, such as Amazon Product and Customer Reviews. The SAAS model, in conjunction with GPT-3, BERT, and T5, undergoes training and testing to evaluate its effectiveness across multiple criteria. The results demonstrate that the SAAS model surpasses other models in delivering contextually aware and pertinent recommendations. The integration process outlines the specific steps involved in capturing, processing, and transforming user interactions in virtual reality (VR) into vocal suggestions provided by non-player characters (NPCs). This strategy improves customization and utilizes the immersive features of virtual reality to effectively engage people. The results of our research establish a higher standard for e-commerce, with the goal of enhancing the user experience of online purchasing by making it more instinctive, engaging, and pleasurable. © 2024 by the authors.

关键词： Data mining

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White Bread Mold Detection using K-Means Clustering Based on Grey Level Co-Occurrence Matrix and Region of Interest

White Bread Mold Detection using K-Means Clustering Based on...

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2023 International Seminar on Application for technology of Information and Communication, iSemantic 2023

作者： Putra, Qori Alfatah Sari, Christy Atika Rachmawanto, Eko Hari Cahyo, Nur Ryan Dwi Mulyanto, Edy Alkhafaji, Mohamed Ayad University of Dian Nuswantoro Study Program in Informatics Engineering Semarang Indonesia University of Dian Nuswantoro Department of Informatics Engineering Semarang Indonesia National University of Science and Technology Department of Computer Science DhiQar Iraq

ISBN: (纸本)9798350339215

This research paper presents a bread mold identification system that utilizes digital image processing techniques, specifically K-means clustering and thresholding, to accurately detect and classify mold-infected areas on bread. The system aims to provide valuable information regarding the safety of consuming mold-contaminated bread, enabling consumers to make informed decisions and reduce the risk of health issues associated with consuming mold-infested bread. the K-means clustering algorithm for mold classification, considering factors such as the number of iterations and the distance metric within the feature space to determine its accuracy. The GLCM feature extraction technique is employed to extract texture information from bread images, encompassing features such as standard deviation (110.62), contrast (1.46), energy, and homogeneity (0.96 and 0.77, respectively). Experimental results demonstrate that the Euclidean Distance yielded the highest result of 72.73, the Cosine Distance obtained the highest result of 8.25, and the City Block Distance obtained the highest result of 92.11. These findings showcase the effectiveness of the system in accurately detecting and classifying mold-infected areas on bread, thereby aiding in ensuring food safety and reducing health risks for consumers. © 2023 IEEE.

关键词： Image segmentation

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Detection and Recognition of Vehicle Components Using Image Processing and Deep Learning Techniques 4

Detection and Recognition of Vehicle Components Using Image ...

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4th Research, Invention, and Innovation Congress: Innovative Electricals and Electronics, RI2C 2023

作者： Ketcham, Mahasak Ganokratanaa, Thittaporn Pramkeaw, Patiyuth Boonthai, Phumraphi Chumuang, Narumol Department of Information Technology Management King Mongkut's University of Technology North Bangkok Bangkok Thailand Applied Computer Science Program King Mongkut’s University of Technology Thonburi Bangkok Thailand Media Technology Program King Mongkut’s University of Technology Thonburi Bangkok Thailand Department Media Technology Muban Chombueng Rajabhat University Ratchaburi Thailand

ISBN: (纸本)9798350330618

This research study presents an algorithmic approach for the detection and identification of key components on license plates, including numerical digits, province codes, vehicle colors, and vehicle makes and types. The methodology integrates image processing techniques and deep learning methodologies to achieve comprehensive image analysis. The algorithm incorporates image preprocessing techniques to optimize the input images, followed by a deep neural network trained on a large annotated dataset. The algorithm utilizes image segmentation and character recognition techniques to detect and classify numerical digits on license plates. Similarly, it employs segmentation and classification processes for identifying province codes, vehicle colors, makes, and types. Extensive training ensures the algorithm's accuracy and robustness under varying conditions. The proposed algorithm demonstrates the potential for accurate license plate component detection and identification, with applications in law enforcement and traffic monitoring systems. . ©2023 IEEE.

关键词： License plates (automobile)

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The Adoption of Artificial Intelligence technology in Parking Security System 7th

The Adoption of Artificial Intelligence Technology in Parkin...

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7th International Conference on Inventive Systems and Control, ICISC 2023

作者： Alhafizh, Muhammad Rifqi Adriawan, Albertus Baskara Yunandito Fernaldy, Darryl Gaol, Ford Lumban Matsuo, Tokuro Information Systems Department School of Information Systems Bina Nusantara University Jakarta11480 Indonesia Computer Science Department BINUS Graduate Program—Doctor of Computer Science Bina Nusantara University Jakarta11480 Indonesia Advanced Institute of Industrial Technology Tokyo Japan

ISBN: (纸本)9789819916238

Artificial intelligence (AI) refers to human-like intelligence exhibited by computers, robots, or other machines. In popular use, artificial intelligence refers to the ability of a computer or machine to mimic the ability of the human mind to learn from examples and experiences, recognize objects, understand and respond to language, make decisions, solve problems, and combine these and other abilities to perform functions that humans might perform. Artificial intelligence requires experience and data to smarten up the technology. The most important things in making artificial intelligence are learning, reasoning, and self-correction. At the learning stage, AI gives machines the ability to learn tasks without requiring a defined programming language. Then, the reasoning stage is the stage where the reason AI is applied in a technology. The self-correction stage is the stage where the AI is refining itself from and learning from experience in order to minimize errors or problems that exist. Then, this matter is concerned with the parking lot to be discussed. Parking can be interpreted as public facilities available in agencies or offices that serve to store vehicles. Vehicles entering the parking area become tens or even thousands, because it requires a parking system and management area. Such arrangements are capable of parking procedures and even other support systems such as adequate parking facilities and infrastructure, and another function is to create and develop parking systems in general to provide safety and comfort. The methodology obtained from this problem is checking the research model to check and find out the comparison of vehicle user’s Parking System Security between cars or motorcycles, as well as the usual parking lots visited. Then, we created a questionnaire. This study used questionnaires with the aim to get respondents’ results about the Parking Security System. Our sampling method is based on respondents aged 17–20 years. Questionnaires are su

关键词： Security systems

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Investing in Products with the Greatest Demand on Online Stores During the Pandemic 7th

Investing in Products with the Greatest Demand on Online Sto...

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7th International Conference on Inventive Systems and Control, ICISC 2023

作者： Hassya, Titan Hanif, Muhammad Fauzi Alvian Gaol, Ford Lumban Matsuo, Tokuro Information Systems Department School of Information Systems Bina Nusantara University Jakarta11480 Indonesia Computer Science Department BINUS Graduate Program—Doctor of Computer Science Bina Nusantara University Jakarta11480 Indonesia Advanced Institute of Industrial Technology Tokyo Japan

ISBN: (纸本)9789819916238

From the year 2019, every aspect of our lives has been negatively impacted as a result of the spread of a deadly disease known as COVID-19, particularly the health and business domains. The primary goal of this study is to develop a solution, or at the very least an overall analysis, to the readers on how to overcome the economic crisis during pandemic. © 2023, The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd.

关键词： COVID-19

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KenCoh: A Ranked-Based Canonical Coherence 1

arXiv

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arXiv 2024年

作者： Talento, Mara Sherlin D.P. Roy, Sarbojit Ombao, Hernando Statistics Program Computer Electrical and Mathematical Science & Engineering King Abdullah University of Science and Technology Saudi Arabia

In this paper, we consider the problem of characterizing a robust global dependence between two brain regions where each region may contain several voxels or channels. This work is driven by experiments to investigate the dependence between two cortical regions and to identify differences in brain networks between brain states, e.g., alert and drowsy states. The most common approach to explore dependence between two groups of variables (or signals) is via canonical correlation analysis (CCA). However, it is limited to only capturing linear associations and is sensitive to outlier observations. These limitations are crucial because brain network connectivity is likely to be more complex than linear and that brain signals may exhibit heavy-tailed properties. To overcome these limitations, we develop a robust method, Kendall canonical coherence (KenCoh), for learning monotonic connectivity structure among neuronal signals filtered at given frequency bands. Furthermore, we propose the KenCoh-based permutation test to investigate the differences in brain network connectivity between two different states. Our simulation study demonstrates that KenCoh is competitive to the traditional variance-covariance estimator and outperforms the later when the underlying distributions are heavy-tailed. We apply our method to EEG recordings from a virtual-reality driving experiment. Our proposed method led to further insights on the differences of frontal-parietal cross-dependence network when the subject is alert and when the subject is drowsy and that left-parietal channel drives this dependence at the beta-band. © 2024, CC BY.

关键词： Statistics

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An Environmentally Friendly Fishing Android-Based Mobile Device App for Penghu Islands (Pescadores), Taiwan

An Environmentally Friendly Fishing Android-Based Mobile Dev...

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Consumer technology (ICCT-Pacific), International Conference on

作者： Hung-Tu Lin Ke-Qing Yan Ying-Zun Fang Xiang-Rui Huang Liang-Bi Chen Department of Computer Science and Information Engineering National Penghu University of Science and Technology Penghu Taiwan Department of Electrical Engineering Master Program in Electrical Engineering and Computer Science National Penghu University of Science and Technology Penghu Taiwan

ISBN: (数字)9798331504120

ISBN: (纸本)9798331504137

Penghu Islands (Pescadores), Taiwan's outlying islands surrounded by the sea, boast rich fishing resources that attract many anglers. However, successful fishing requires more than just gear: it involves understanding the varying fish species available in different locations, times, and climates, as well as adhering to relevant laws and regulations. To address these needs, this paper proposes an environmentally friendly fishing Android-based mobile device App specifically for the Penghu Islands. The proposed Android-based mobile device App aims to gather essential information, including local fishing regulations, aquatic safety tips, and important precautions. Its goal is to equip fishermen with the resources necessary to fish successfully and with peace of mind.

关键词： Sea measurements Length measurement Fish Regulation Mobile applications Tides Protection Smart phones Meteorology Lenses

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Enhancing Geometric Ontology Embeddings for EL++ with Negative Sampling and Deductive Closure Filtering

arXiv

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arXiv 2024年

作者： Mashkova, Olga Zhapa-Camacho, Fernando Hoehndorf, Robert Computer Science Program Computer Electrical and Mathematical Sciences & Engineering Division King Abdullah University of Science and Technology Thuwal23955 Saudi Arabia

Ontology embeddings map classes, relations, and individuals in ontologies into Rn, and within Rn similarity between entities can be computed or new axioms inferred. For ontologies in the Description Logic EL++, several embedding methods have been developed that explicitly generate models of an ontology. However, these methods suffer from some limitations;they do not distinguish between statements that are unprovable and provably false, and therefore they may use entailed statements as negatives. Furthermore, they do not utilize the deductive closure of an ontology to identify statements that are inferred but not asserted. We evaluated a set of embedding methods for EL++ ontologies based on high-dimensional ball representation of concept descriptions, incorporating several modifications that aim to make use of the ontology deductive closure. In particular, we designed novel negative losses that account both for the deductive closure and different types of negatives. We demonstrate that our embedding methods improve over the baseline ontology embedding in the task of knowledge base or ontology completion. © 2024, CC BY.

关键词： Ontology

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Machine Learning-Based Classification of Suicidal Ideation in Tweets: A Sentiment Analysis Approach

Machine Learning-Based Classification of Suicidal Ideation i...

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International Conference on Informatics, Multimedia, Cyber and Information System (ICIMCIS)

作者： Orsino Jerrell Bruinier Irma Kartika Wairooy Vladimir Elwin Leonard Brilly Andro Makalew Computer Science Department School of Computer Science Bina Nusantara University Jakarta Indonesia Computer Science Department Mobile Application & Technology Program Bina Nusantara University Jakarta Indonesia

ISBN: (数字)9798331533243

ISBN: (纸本)9798331533250

Suicide appears to have reached the highest public health importance, with social media like Twitter providing an infinite source of information that may help in the detection of ideation. This paper describes the development of an effective machine learning-based model for the classification of suicidal ideation in tweets using sentiment analysis. An assembly was done with a dataset of 9,120 tweets; they were labelled according to the suicidal thoughts expressed in them. We presented the assembly and preprocessing of a dataset consisting of 9,120 tweets designated based on indicative or non-indicative suicidal thoughts. The preprocessing steps consisted of normalization, tokenization, stop word removal, and lemmatization. Five ML models, namely Support Vector Machine (SVM), Multinomial Naive Bayes (MNB), Random Forest (RF), Stochastic Gradient Descent (SGD), and Logistic Regression (LR), were further trained on the selected dataset. The models are tested for standard classification metrics: accuracy, precision, recall, F1-Score, and AUC. The best performance for the model was Random Forest with 95.135% and an F1-Score of 95.094%. In conclusion, our study established that an amalgamation of sentiment analysis in ML models can have the potential to detect early suicidal tendencies and that it lays a platform for timely interventions. Future research should be focused on making the dataset even larger and enhancing the detection of sarcasm and jokes in the models.

关键词： Support vector machines Radio frequency Sentiment analysis Analytical models Accuracy Social networking (online) Blogs Training data Random forests Assembly

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