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SSRN 2022年

作者： Wang, Nan Richardson, Ruth E. Mark, Noah Launer, Nathaniel Hirtler, Adrianna Weston, Claire LaGorga, Lydia Cleckner, Lisa Faehndrich, Chloe Penningroth, Stephen M. Xia, Lingzi Pyrek, Daniel Civil and Environmental Engineering Cornell University IthacaNY14853 United States Community Science Institute IthacaNY14850 United States New York State Department of Health-Environmental Laboratory Approval Program ID 11790 United States Finger Lakes Institute Hobart and William Smith Colleges GenevaNY United States Skidmore College Environmental Studies and Sciences Program Saratoga SpringsNY12866 United States Civil and Environmental Engineering Cornell University IthacaNY14853 United States

The Finger Lakes (FLs) region of NY has seen a steep increase in occurrences of cyanobacterial harmful algal blooms (cHABs) over the last decade. Since 2016, local volunteer groups have been established in a grassroots effort to monitor blooms across the FLs. One of the FLs, Cayuga Lake (CY), had the most reported cHABs of all NY water bodies in 2020. The Cayuga Lake HABs Monitoring program, initiated by local non-profit organizations in 2018 and implemented annually from June to October on CY, recruited and trained "HABs Harrier" volunteers to collect and document suspicious blooms and deliver samples to CSI’s state-certified lab for measurement of chlorophyll a and microcystin as well as microscopic identification of cyanobacteria taxa. Volunteers patrolled slightly >50% of the 96 miles of CY shoreline in 2020. Patterns from three years of data collected by the Cayuga Lake HABs Monitoring program (2018-2020) showed that cHABs were dominated by the genus Microcystis, and a strong correlation was observed between the concentrations of MC and chlorophyll A if Microcystis was present. A subset of bloom samples was split for analysis at the Cornell group using molecular biological tools: qPCR for targeted detection/quantification of the microcystin synthase gene A ( mcyA ) and high throughput 16S rDNA community analysis. The 16S rDNA community analysis confirmed the results of microscopic analysis that Microcystis was dominant in high toxin cHABs. The qPCR devices (one bench-top and one handheld (field scale) qPCR device) showed reasonable specificity (60%-75%) and high sensitivity (87.5-89.5%) for the presence of high toxin (defined as >4 µg/L MC by the New York State Department of Health for public bathing beaches). Rapid screening tools such as qPCR could provide on-site decision tools to inform public health staff in managing risk from high microcystin blooms. © 2022, The Authors. All rights reserved.

关键词： Lakes

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A First-Principles and Calphad-Assisted Phase-Field Model for Microstructure Evolution: Application to Mo-V Binary Alloy Systems

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SSRN 2023年

作者： Thakur, Abhishek Kumar Kovacevic, Sasa Manga, Venkateswara Rao Deymier, Pierre Alix Muralidharan, Krishna Department of Materials Science and Engineering University of Arizona TucsonAZ85721 United States Department of Civil and Environmental Engineering Imperial College London LondonSW7 2AZ United Kingdom Lunar and Planetary Laboratory University of Arizona TucsonAZ85721 United States Graduate Interdisciplinary Program in Applied Mathematics India

A multiscale computational framework combining the first-principles calculations and the CALPHAD approach with the phase-field method is presented to simulate the microstructure evolution in multicomponent steel alloys. We demonstrate the potential of the framework by predicting the microstructural evolution in elastically periodic arrays of the Mo-V binary sub-system. The framework utilizes the first-principles calculations using special quasi-random structures. Hitherto unavailable thermodynamic and material properties of the alloy are obtained by employing the first-principles calculations and the CALPHAD approach and fed into the phase-field model to predict the microstructure evolution at different temperatures within the miscibility gap region. In addition to the temperature and cooling rates, the model incorporates the role of mechanical fields in decomposition kinetics in the Mo-V binary alloy system. Regimes for temperatures and cooling rates at which spinodal decomposition occurs are identified. Applying external loading leads to directional phase separation in the Mo-V binary system. The elastic inhomogeneity in terms of material properties between the two phases initiates the directional alignment while eigenstrains and applied external loading control the degree of alignment. The framework developed is general and extendable to higher multicomponent sub-systems in steel alloys. © 2023, The Authors. All rights reserved.

关键词： Temperature

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Groundwater-Surface Water Interactions Research: Past Trends and Future Directions

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SSRN 2024年

作者： Irvine, Dylan J. Singha, Kamini Kurylyk, Barret L. Briggs, Martin A. Sebastian, Yakub Tait, Douglas R. Helton, Ashley M. Research Institute for the Environment and Livelihoods Faculty of Science and Technology Charles Darwin University CasuarinaNT Australia National Centre for Groundwater Research and Training Bedford ParkSA Australia Hydrologic Science and Engineering Program Colorado School of Mines GoldenCO United States Department of Civil and Resource Engineering Centre for Water Resources Studies Dalhousie University Halifax Canada U.S. Geological Survey Observing Systems Division Hydrologic Remote Sensing Branch StorrsCT United States Faculty of Science and Engineering Southern Cross University Lismore Australia Department of Natural Resources and the Environment The Center for Environmental Sciences and Engineering University of Connecticut StorrsCT United States

Interactions between groundwater and surface water sustain groundwater-dependent ecosystems, provide baseflow to rivers, and regulate river temperature and biogeochemical cycles, amongst many other processes. These interactions occur in freshwater environments including rivers, springs, lakes, and wetlands, and in coastal environments via tidal pumping, submarine groundwater discharge, and seawater intrusion. Here, we use a data-driven approach to explore groundwater-surface water interactions research using 20,275 journal papers published between 1970 and 2023. Analyses show that research into groundwater-surface water interactions is highly multi-disciplinary, with growing contributions from the social and biological sciences. As in the case with many research areas, the number of groundwater-surface water interactions papers is rapidly increasing with over 1200 papers published per year since 2020. Drawing on our data-driven approach and expert knowledge, we synthesise current research trends and identify critical future research directions. Despite the thousands of papers on groundwater-surface water interactions, there are still important processes that are difficult to quantify or predict at meaningful spatial scales to inform management. We see benefits in future groundwater-surface water interactions research focusing on: (1) embracing new technologies including the use of internet-of-things-based sensors and further use of uncrewed vehicles in data capture and remote sensing approaches to inform groundwater-surface water interactions at large scales, (2) capitalising on the increased computational power, availability of physically based models, and machine learning to inform hydrological and biogeochemical processes, and (3) continuing the movement towards multi-disciplinary investigations to better inform the quantification of groundwater-surface water interactions that will enable better management outcomes. © 2024, The Authors. All rights reserved.

关键词： Rivers

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Halobacterium salinarum NRC-1 Sustains Voltage Production in a Dual-Chambered Closed Microbial Fuel Cell

Scientific World Journal

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Scientific World Journal 2022年第1期2022卷 3885745页

作者： Goncalves, Rodrigo Oliveira Salehi, Ali Publico, Marlon Nyende, Jimmy Nadarajah, Nalina Ghoreyshi, Soheil Shastri, Padmaja Biomedical Engineering Program Applied Biological and Environmental Science Centennial College 941 Progress Avenue Scarborough M1G 3T8 Canada Biotechnology Program Applied Biological and Environmental Science Centennial College 755 Morningside Avenue Scarborough M1C 4Z4 Canada

Sustained bioenergy production from organisms that thrive in high salinity, low oxygen, and low nutrition levels is useful in monitoring hypersaline polluted environments. Microbial fuel cell (MFC) studies utilizing single species halophiles under salt concentrations higher than 1 M and as a closed microbial system are limited. The current study aimed to establish baseline voltage, current, and power density from a dual-chambered MFC utilizing the halophile Halobacterium salinarum NRC-1. MFC performance was determined with two different electrode sizes (5 cm2 and 10 cm2), under oscillating and nonoscillating conditions, as well as in a stacked series. A closed dual-chamber MFC system of 100 mL capacity was devised with Halobacterium media (4.3 M salt concentration) as both anolyte and catholyte, with H. salinarum NRC-1 being the anodic organism. The MFC measured electrical output over 7, 14, 28, and 42 days. MFC output increased with 5 cm2 sized electrodes under nonoscillating (p<0.0001) relative to oscillating conditions. However, under oscillating conditions, doubling the electrode size increased MFC output significantly (p=0.01). The stacked series MFC, with an electrode size of 10 cm2, produced the highest power density (1.2672 mW/m2) over 14 days under oscillation. Our results highlight the potentiality of H. salinarum as a viable anodic organism to produce sustained voltage in a closed-MFC system. © 2022 Rodrigo Oliveira Goncalves et al.

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Anti-inflammatory and antinociceptive effects of Aloysia gratissima leaves essential oil: An in vivo study

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Journal of Traditional and Complementary Medicine 2024年

作者： Souza, Maryelen A. Kunh, Ketelin Sanaiotto, Otávio Provinelli, Ana C. Barufke, Mayara Schindler, Monica S.Z. Mazon, Samara Cristina Oliveira, J. Vladimir Brusco, Indiara Scapinello, Jaqueline Magro, Jacir Dal Müller, Liz G. Graduate Program in Environmental Sciences Community University of Chapecó Region (Unochapecó) Chapecó Brazil School of Health Sciences Community University of Chapecó Region (Unochapeco) Chapecó Brazil Department of Chemical and Food Engineering Federal University of Santa Catarina (UFSC) SC Florianópolis Brazil Graduate Program in Food Science and Technology - State University of Santa Catarina - UDESC Pinhalzinho Brazil

Background and aim: Aloysia gratissima is used in popular medicine as an analgesic and sedative. However, studies on its biological activities are still scarce. Therefore, this work aimed to evaluate the antinociceptive and anti-inflammatory effect of A. gratissima leaves essential oil (OAG) in mice. Experimental procedure: The OAG was obtained by hydrodistillation and its composition was determined by gas chromatography-mass spectrometry. The sesquiterpenes pinocamphone, β-pinene, and guaiol were the major constituents of OAG. The effect of OAG (1, 10, and 30 mg/kg, p.o.) was assessed by pre-treating male mice 1h before the formalin assay, carrageenan-induced peritonitis test, or the paw edema induced by arachidonic acid (AA). The acute toxicity of OAG (2000 mg/kg, p.o.) was also investigated. Results and conclusion: The minimum effective dose of OAG in the formalin test was 1 mg/kg. This dose did not affect the locomotor activity of mice. The OAG decreased the number of leukocytes/mm³ in the peritoneal exudate of mice and reduced paw edema 45 min after the arachidonic acid injection. OAG treatment elicited a reduction in NPSH levels in the paw tissue and increased NPSH levels in mice blood plasma and did not cause mice mortality. The OAG is devoid of acute toxicity and shows anti-inflammatory activity, which can be related to the presence of pinocamphone, guaiol, and β-pinene and is mediated, at least in part, by mechanisms that involve the participation of NPSH. © 2024 Center for Food and Biomolecules, National Taiwan University

关键词： Acute toxicity test Anti-inflammatory agents Antinociceptive agents Natural products Verbenaceae

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A monoclonal antibody against Lates calcarifer vitellogenin and a competitive ELISA to evaluate vitellogenin induction after exposure to xenoestrogen

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Journal of environmental sciences 2019年第1期31卷 325-333页

作者： Witchuda Prasatkaew Phochit Nanthanawat Nanthika Khongchareonporn Sutin Kingtong Environmental Science program Faculty of Science Burapha University Department of Biotechnology Faculty of Science Burapha University The Institute of Biotechnology and Genetic Engineering Chulalongkorn University Department of Biology Faculty of Science Burapha University

A monoclonal antibody specific to sea bass(Lates calcarifer) vitellogenin(VTG) was developed,for use as a tool for monitoring endocrine disrupting chemicals(EDCs). VTG was induced in sea bass by intramuscular injection of 17β-estradiol(E_2: 2 mg/kg) every three days. Blood was collected three days after the last injection. Plasma VTG was then purified by chromatography in hydroxyapatite and a sephacryl-S300 column. Characterizations of purified VTG were done by phospholipoglycoprotein staining on a native-PAGE with confirmation by mass spectrometry(LC-MS/MS). Antibody was raised in mice by injection of purified VTG. After monoclonal antibody production, the hybridoma clone No. 41(MAb-sea bass VTG 41)was selected and developed for quantification of VTG by competitive enzyme-linked immunosorbent assay(ELISA). The ELISA method was sensitive with a detection limit of VTG 40 ng/mL. MAb-sea bass VTG 41 was specific to VTG from E_2-treated sea bass and others EDCs(Nonylphenol, Benzo[a]pyrene and CdCl_2). Moreover, cross-reactivity was also found in E_2-treated coral grouper(Epinephelus corallicola). The ELISA method obtained from this work can be further applied for the assessment of EDCs in Thailand and Southeast Asia's aquatic environment.

关键词： Monoclonal antibody Vitellogenin Biomarker Endocrine disrupting chemicals(EDCs) ELISA

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Simulated instream restoration structures offer smallmouth bass (Micropterus dolomieu) swimming and energetic advantages at high flow velocities

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Canadian Journal of Fisheries and Aquatic sciences 2021年第1期78卷 40-56页

作者： Strailey, Katherine K. Osborn, Ryan T. Tinoco, Rafael O. Cienciala, Piotr Rhoads, Bruce L. Suski, Cory D. Program in Ecology Evolution and Conservation Biology University of Illinois at Urbana-Champaign Urbana 61801 IL United States Department of Civil and Environmental Engineering Virginia Polytechnic Institute and State University Blacksburg 24061 VA United States Department of Civil and Environmental Engineering University of Illinois at Urbana-Champaign Urbana 61801 IL United States Department of Geography and Geographic Information Science University of Illinois at Urbana-Champaign Urbana 61801 IL United States Department of Natural Resources and Environmental Sciences University of Illinois at Urbana-Champaign Urbana 61801 IL United States

Restoration practices aimed at fish habitat enhancement often include installation of instream structures. However, mixed outcomes have been reported regarding structure effectiveness, while mechanisms underlying success remain unclear. The interactions between fish and flow conditions generated by instream structures and their subsequent impact on fish energetics may provide some insight. This study seeks to quantify how restoration structures, simulated by cylinders in three orientations, alter the energetics and swimming stability of smallmouth bass (Micropterus dolomieu). Accelerometers measured swimming stability while a respirometer measured energy expenditure at multiple velocities. Particle image velocimetry was used to characterize flow fields behind structures. Structures generated flow conditions that benefited fish energetically. Fish had a smoother gait and expended less energy when swimming near a structure, regardless of its orientation. Benefits varied with flow conditions;reductions in energy expenditure were especially apparent at high flow velocities. Results suggest that restoration structures may be most energetically beneficial in stream systems with consistently high velocities and inform restoration by indicating flow conditions in which structures provide the greatest energetic benefits for fish. © Canadian science Publishing. All rights reserved.

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Z-scheme S, B co-doped g-C3N4 nanotube@MnO2 heterojunction with visible-light-responsive for enhanced photodegradation of diclofenac by peroxymonosulfate activation

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Chemical engineering Journal 2023年 452卷

作者： Dung Nguyen, Manh Binh Nguyen, Thanh Hai Tran, Linh Giang Nguyen, Thuy Fatimah, Is Prasetyo Kuncoro, Eko Doong, Ruey-An Department of Biomedical Engineering and Environmental Sciences National Tsing Hua University Hsinchu30013 Taiwan Institute of Aquatic Science and Technology National Kaohsiung University of Science and Technology Kaohsiung City81157 Taiwan Center of Crop Research for Adaptation to Climate Change Thai Nguyen University of Agriculture and Forestry Quyet Thang Ward Thai Nguyen City Viet Nam Department of Chemistry Faculty of Mathematics and Natural Science Universitas Islam Indonesia Kampus Terpadu UII J1. Kaliurang Km 14 Sleman Yogyakarta Indonesia Environmental Engineering Program Faculty of Science and Technology University of Airlangga Surabaya60115 Indonesia Institute of Analytical and Environmental Sciences National Tsing Hua University 101 Sec. 2 Kuang Fu Road Hsinchu30013 Taiwan

In this study, the manganese dioxide (MnO2) nanosheets were successfully embedded onto boron and sulfur co-doped g-C3N4 (CNBS) nanotubes to construct the flower-like core–shell CNBS@MnO2 heterojunction with Z-scheme structure for the enhanced photodegradation of diclofenac (DCF) in the presence of peroxymonosulfate (PMS) and 460-nm visible light. The CNBS@MnO2 provides large specific surface area and reduced interfacial resistance for rapid electron transport. Moreover, the redox cycling of Mn species effectively activates PMS to produce sulfate radicals for DCF photodegradation. The PMS-based photodegradation of DCF over CNBS@MnO2 displays the excellent photodegradation performance after 15 min of irradiation, and the efficiency is highly dependent on initial pH, ion species, and concentrations of PMS and DCF. The CNBS@MnO2 also shows its excellent stability and reusability over ten cycles. Besides, h+, O2●− and SO4●− are the dominantly reactive species for the enhanced degradation of DCF over Z-scheme CNBS@MnO2 heterojunction. The possible reaction mechanism as well as the degradation pathway of DCF over CNBS@MnO2 in the presence of PMS is also proposed. Results clearly highlight the superior photoactivity of Z-scheme CNBS@MnO2 toward micropollutant degradation by PMS activation, which can open an avenue to fabricate the g-C3N4 nanotube@metal oxide structure as an effective activator for water and wastewater purification. © 2022 Elsevier B.V.

关键词： Heterojunctions

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CIWARS: A Web Server for Antibiotic Resistance Surveillance Using Longitudinal Metagenomic Data

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Journal of Molecular Biology 2025年第15期437卷

作者： Emon, Muhit Islam Cheung, Yat Fei Stoll, James Rumi, Monjura Afrin Brown, Connor Choi, Joung Min Moumi, Nazifa Ahmed Ahmed, Shafayat Song, Haoqiu Sein, Justin Yao, Shunyu Khan, Ahmad Gupta, Suraj Kulkarni, Rutwik Butt, Ali Vikesland, Peter Pruden, Amy Zhang, Liqing Department of Computer Science Virginia Tech Blacksburg 24060 VA United States Fralin Life Science Institute Virginia Tech Blacksburg 24060 VA United States Department of Civil and Environmental Engineering Virginia Tech Blacksburg 24060 VA United States The Interdisciplinary PhD Program in Genetics Bioinformatics and Computational Biology Virginia Tech Blacksburg 24060 VA United States

The rise of antibiotic resistance (AR) poses a substantial threat to human and animal health, food security, and economic stability. Wastewater-based surveillance (WBS) has emerged as a powerful strategy for population-level AR monitoring, providing valuable data to guide public health and policy decisions. Metagenomic sequencing is especially promising, as it can yield comprehensive profiles of antibiotic resistance genes (ARGs) and other genes relevant to AR in a single run. However, online analytical platforms to facilitate analysis of longitudinal metagenomic data are lacking. To address this, we introduce CyberInfrastructure for Waterborne Antibiotic Resistance Surveillance (CIWARS), a web server configured for characterizing key AR trends from longitudinal metagenomic WBS data. CIWARS offers comprehensive profiling of ARGs and taxonomic profiling of pathogen-associated bacterial taxonomic groups, identifies potential associations of ARGs with mobile genetic elements (MGEs) and pathogen-containing taxa, and assesses resistome risk based on the co-occurrence of ARGs, MGEs, and pathogen-like sequences. Additionally, it detects anomalous AR indicators over time, aiding in identifying potential events of concern, such as the emergence of resistant strains or outbreaks. Through interactive temporal data visualization, CIWARS enables AR monitoring and can serve as a tool to inform effective and timely interventions to mitigate the spread and transmission of AR. Here, CIWARS is demonstrated using longitudinal metagenomic data from a wastewater treatment plant (WWTP) influent and effluent, but it can be extended to any environment. CIWARS provides a valuable tool to support global efforts to combat the evolution and spread of AR, while also guiding agricultural and public health efforts aimed at optimizing antibiotic use. The web server is freely available at https://***/. © 2025 Elsevier Ltd

关键词： anomaly detection antibiotic resistance (AR) AR indicator profiling longitudinal metagenomics wastewater-based surveillance

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Cyclodextrin-Modified Layered Double Hydroxide Thin-Film Nanocomposite Desalination Membrane for Boron Removal

SSRN

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SSRN 2023年

作者： Ee, Liang Ying Zhao, Qipeng Gao, Jie Lim, Chit Kai Xue, Kai Chin, Sze Yuet Li, Sam Fong Yau Chung, Tai-Shung Chen, Shing Bor Department of Chemical and Biomolecular Engineering College of Design and Engineering National University of Singapore 4 Engineering Drive 4 Singapore117585 Singapore Department of Chemistry College of Humanities and Sciences National University of Singapore 3 Science Drive 3 Singapore117543 Singapore Chemical Engineering Program Physical Science and Engineering Division King Abdullah University of Science and Technology Thuwal23955-6900 Saudi Arabia Centre of High Field NMR Spectroscopy and Imaging School of Physical and Mathematical Sciences College of Science Nanyang Technological University 21 Nanyang Link Singapore637371 Singapore NUS Environmental Research Institute National University of Singapore T-Lab Building 5A Engineering Drive 1 Singapore117411 Singapore Graduate Institute of Applied Science and Technology National Taiwan University of Science and Technology Taipei106335 Taiwan

The elimination of small and potentially toxic boron species in water is always a significant challenge for conventional membrane technologies. In this study, facile intercalation and functionalization of β-cyclodextrin (CD) onto layered double hydroxides (LDHs) have resulted in the formation of accessible hydroxyl functional groups and anions that exhibit strong sorption with boron. Through meticulous molecular design and optimization, the modified LDH possessing the maximum boron adsorption capacity of 96.1 mg g-1 was employed to fabricate a polyamide-based thin-film nanocomposite (TFN) membrane with a loading of 0.10 wt.%. This resultant membrane displayed a high salt rejection and water permeance of 99.4 % and 2.68 LMH bar-1 for brackish water, respectively, which can be attributed to the increased interlayer spacing of 1.67 nm and the thinner selective layer. The innovative TFN membrane also achieved a high boron rejection of 82.3 % against brackish water containing 2,000 mg L-1 NaCl and 15 mg L-1 B at pH 8. This pioneering study provides valuable insights into the design of brackish water reverse osmosis (BWRO) membranes, through the synergistic use of macrocyclic molecules and inorganic layered nanomaterials, that could potentially revolutionize water reuse and boron removal applications. © 2023, The Authors. All rights reserved.

关键词： Sodium chloride

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