检索结果-内蒙古大学图书馆

Α γ-tubulin complex-dependent pathway suppresses ciliogenesis by promoting cilia disassembly

Cell Reports 2022年第7期41卷 000-000页

作者： Shankar, Sahana Hsu, Zi-Ting Ezquerra, Artur Li, Chien-Chien Huang, Tzu-Lun Coyaud, Etienne Viais, Ricardo Grauffel, Cédric Raught, Brian Lim, Carmay Lüders, Jens Tsai, Su-Yi Hsia, Kuo-Chiang Molecular and Cell Biology Taiwan International Graduate Program Academia Sinica and National Defense Medical Center Taipei 11490 Taiwan Institute of Molecular Biology Academia Sinica Taipei 11529 Taiwan Department of Life Science National Taiwan University Taipei 10617 Taiwan Institute for Research in Biomedicine (IRB Barcelona) The Barcelona Institute of Science and Technology Barcelona 08028 Spain Princess Margaret Cancer Centre University Health Network 101 College Street Toronto M5G 1L7 ON Canada Department of Medical Biophysics University of Toronto Toronto M5G 1L7 ON Canada Institute of Biomedical Sciences Academia Sinica Taipei Taiwan Research Center for Developmental Biology and Regenerative Medicine National Taiwan University Taipei 10617 Taiwan Genome and Systems Biology Degree Program National Taiwan University and Academia Sinica Taipei 10617 Taiwan Institute of Biochemistry and Molecular Biology College of Life Sciences National Yang-Ming University Taipei 11221 Taiwan

The primary cilium, a microtubule-based sensory organelle, undergoes cycles of assembly and disassembly that govern the cell cycle progression critical to cell proliferation and differentiation. Although cilia assembly has been studied extensively, the molecular mechanisms underlying cilia disassembly are less well understood. Here, we uncover a γ-tubulin ring complex (γ-TuRC)-dependent pathway that promotes cilia disassembly and thereby prevents cilia formation. We further demonstrate that Kif2A, a kinesin motor that bears microtubule-depolymerizing activity, is recruited to the cilium basal body in a γ-TuRC-dependent manner. Our mechanistic analyses show that γ-TuRC specifically recruits Kif2A via the GCP2 subunit and its binding partner Mzt2. Hence, despite the long-standing view that γ-TuRC acts mainly as a microtubule template, we illustrate that its functional heterogeneity at the basal body facilitates both microtubule nucleation and Kif2A recruitment-mediated regulation of ciliogenesis, ensuring cell cycle progression. © 2022 The Author(s)

关键词： cell cycle ciliogenesis CP: Molecular biology kinesin motor microtubule primary cilium γ-tubulin ring complex

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Dynamic phenotypic heterogeneity and the evolution of multiple RNA subtypes in hepatocellular carcinoma:the PLANET study

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National science Review 2022年第3期9卷 96-109页

作者： Weiwei Zhai Hannah Lai Neslihan Arife Kaya Jianbin Chen Hechuan Yang Bingxin Lu Jia Qi Lim Siming Ma Sin Chi Chew Khi Pin Chua Jacob Josiah Santiago Alvarez Pauline Jieqi Chen Mei Mei Chang Lingyan Wu Brian K.P.Goh Alexander Yaw-Fui Chung Chung Yip Chan Peng Chung Cheow Ser Yee Lee Juinn Huar Kam Alfred Wei-Chieh Kow Iyer Shridhar Ganpathi Rawisak Chanwat Jidapa Thammasiri Boon Koon Yoong Diana Bee-Lan Ong Vanessa H.de Villa Rouchelle D.Dela Cruz Tracy Jiezhen Loh Wei Keat Wan Zeng Zeng Anders Jacobsen Skanderup Yin Huei Pang Krishnakumar Madhavan Tony Kiat-Hon Lim Glenn Bonney Wei Qiang Leow Valerie Chew Yock Young Dan Wai Leong Tam Han Chong Toh Roger Sik-Yin Foo Pierce Kah-Hoe Chow Genome Institute of Singapore Agency for Science Technology and Research Key Laboratory of Zoological Systematics and Evolution Institute of Zoology Chinese Academy of Sciences Center for Excellence in Animal Evolution and Genetics Chinese Academy of Sciences School of Biological Sciences Nanyang Technological University Division of Biosciences Faculty of Life Sciences Division of Surgery and Surgical Oncology National Cancer Centre Department of Hepato-Pancreato-Biliary and Transplant Surgery Singapore General Hospital Division of Hepatobiliary and Pancreatic Surgery Department of Surgery University Surgical Cluster National University Health System Hepato-Pancreato-Biliary Surgery Unit Department of Surgery National Cancer Institute Division of Pathology National Cancer Institute Department of Surgery Faculty of Medicine University of Malaya Department of Surgery and Center for Liver Disease Management and Transplantation The Medical City Department of Laboratories The Medical City Department of Pathology Singapore General Hospital Institute for Infocomm Research ASTAR Department of Pathology National University Health System Translational Immunology Institute (TII) Sing Health Duke-NUS Academic Medical Centre Division of Gastroenterology and Hepatology University Medicine Cluster National University Hospital Department of Biochemistry Yong Loo Lin School of Medicine National University of Singapore Cancer Science Institute of Singapore National University of Singapore Division of Medical Oncology National Cancer Center Singapore Cardiovascular Research Institute National University of Singapore National University Healthcare System Singhealth-Duke-NUS Academic Surgery Program Duke-NUS Graduate Medical School Institute of Molecular and Cell Biology Agency for Science Technology and Research

Intra-tumor heterogeneity(ITH) is a key challenge in cancer treatment, but previous studies have focused mainly on the genomic alterations without exploring phenotypic(transcriptomic and immune)heterogeneity. Using one of the largest prospective surgical cohorts for hepatocellular carcinoma(HCC)with multi-region sampling, we sequenced whole genomes and paired transcriptomes from 67 HCC patients(331 samples). We found that while genomic ITH was rather constant across stages, phenotypic ITH had a very different trajectory and quickly diversified in stage Ⅱ patients. Most strikingly, 30% of patients were found to contain more than one transcriptomic subtype within a single tumor. Such phenotypic ITH was found to be much more informative in predicting patient survival than genomic ITH and explains the poor efficacy of single-target systemic therapies in HCC. Taken together, we not only revealed an unprecedentedly dynamic landscape of phenotypic heterogeneity in HCC, but also highlighted the importance of studying phenotypic evolution across cancer types.

关键词： hepatocellular carcinoma intra-tumor heterogeneity phenotypic evolution tumor evolution

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Quantum simulation of an extended Dicke model with a magnetic solid

Communications Materials

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Communications Materials 2024年第1期5卷 42页

作者： Marquez Peraca, Nicolas Li, Xinwei Moya, Jaime M. Hayashida, Kenji Kim, Dasom Ma, Xiaoxuan Neubauer, Kelly J. Fallas Padilla, Diego Huang, Chien-Lung Dai, Pengcheng Nevidomskyy, Andriy H. Pu, Han Morosan, Emilia Cao, Shixun Bamba, Motoaki Kono, Junichiro Department of Physics and Astronomy Rice University HoustonTX United States Department of Physics California Institute of Technology PasadenaCA United States Applied Physics Graduate Program Smalley–Curl Institute Rice University HoustonTX United States Division of Applied Physics Graduate School of Engineering Hokkaido University Hokkaido Japan Department of Electrical and Computer Engineering Rice University HoustonTX United States Department of Physics International Center of Quantum and Molecular Structures and Materials Genome Institute Shanghai University Shanghai China Department of Physics and Center for Quantum Frontiers of Research and Technology National Cheng Kung University Tainan Taiwan Department of Chemistry Rice University HoustonTX United States Department of Physics I Kyoto University Kyoto Japan The Hakubi Center for Advanced Research Kyoto University Kyoto Japan PRESTO Japan Science and Technology Agency Saitama Japan Department of Physics Yokohama National University Yokohama Japan Department of Materials Science and NanoEngineering Rice University HoustonTX United States

The Dicke model describes the cooperative interaction of an ensemble of two-level atoms with a single-mode photonic field and exhibits a quantum phase transition as a function of light–matter coupling strength. Extending this model by incorporating short-range atom–atom interactions makes the problem intractable but is expected to produce new physical phenomena and phases. Here, we simulate such an extended Dicke model using a crystal of ErFeO3, where the role of atoms (photons) is played by Er3+ spins (Fe3+ magnons). Through terahertz spectroscopy and magnetocaloric effect measurements as a function of temperature and magnetic field, we demonstrated the existence of a novel atomically ordered phase in addition to the superradiant and normal phases that are expected from the standard Dicke model. Further, we elucidated the nature of the phase boundaries in the temperature–magnetic-field phase diagram, identifying both first-order and second-order phase transitions. These results lay the foundation for studying multiatomic quantum optics models using well-characterized many-body solid-state systems. © The Author(s) 2024.

关键词： Atoms

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Cryo-EM structure of a tetrameric photosystem I from Chroococcidiopsis TS-821, a thermophilic, unicellular, non-heterocyst-forming cyanobacterium

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Plant Communications 2022年第1期3卷 53-70页

作者： Dmitry A.Semchonok Jyotirmoy Mondal Connor J.Cooper Katrina Schlum Meng Li Muhamed Amin Carlos O.S.Sorzano Erney Ramirez-Aportela Panagiotis L.Kastritis Egbert J.Boekema Albert Guskov Barry D.Bruce Groningen Biomolecular Sciences&Biotechnology Institute University of GroningenGroningenthe Netherlands Biochemistry&Cellular and Molecular Biology Department University of TennesseeKnoxvilleTNUSA Program in Genome Science and Technology University of TennesseeKnoxvilleTNUSA Bredesen Center for Interdisciplinary Research&Education University of TennesseeKnoxvilleTNUSA Department of Sciences University College GroningenGroningenthe Netherlands Biocomputing Unit National Center for Biotechnology(CSIC)Darwin 3Campus Universidad Auto´noma de MadridCantoblanco28049 MadridSpain Universidad CEU San Pablo Campus Urb.Monteprı´ncipeBoadilla del Monte28668 MadridSpain Institute of Biochemistry and Biotechnology Martin Luther University Halle-WittenbergHalle/SaaleGermany Microbiology Department University of TennesseeKnoxvilleTNUSA Present address:Institute of Biochemistry and Biotechnology Martin Luther University Halle-WittenbergHalle/SaaleGermany School of Oceanography University of WashingtonSeattleWAUSA

Photosystem I(PSI)is one of two photosystems involved in oxygenic *** of cyanobacteria exists in monomeric,trimeric,and tetrameric forms,in contrast to the strictly monomeric form of PSI in plants and *** tetrameric organization raises questions about its structural,physiological,and evolutionary *** we report the3.72 A˚resolution cryo-electron microscopy structure of tetrameric PSI from the thermophilic,unicellular cyanobacterium Chroococcidiopsis *** structure resolves 44 subunits and 448 cofactor *** conclude that the tetramer is arranged via two different interfaces resulting from a dimer-of-dimers *** localization of chlorophyll molecules permits an excitation energy pathway within and between adjacent *** analysis reveals conserved regions in the PsaL subunit that correlate with the oligomeric *** PSI may function as a key evolutionary step between the trimeric and monomeric forms of PSI organization in photosynthetic organisms.

关键词： cryo-EM photosystem I non-heterocyst-forming cyanobacteria Chroococcidiopsis evolution of chloroplast high light adaptation

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Ophthalmic characteristics and retinal vasculature changes in Williams syndrome, and its association with systemic diseases

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Eye (Basingstoke) 2023年第11期37卷 2265-2271页

作者： Yeh, Tsai-Chu Cheng, Hui-Chen Li, Hsing-Yuan Chi, Sheng Chu Yang, Hsin-Yu Yu, Jenn-Yah Niu, Dau-Ming Wang, An-Guor School of Medicine National Yang Ming Chiao Tung University Taipei Taiwan Department of Ophthalmology Taipei Veterans General Hospital Taipei Taiwan Department of Ophthalmology School of Medicine National Yang Ming Chiao Tung University Taipei Taiwan Program in Molecular Medicine College of Life Sciences National Yang Ming Chiao Tung University Taipei Taiwan Department of Life Sciences and Institute of Genome Sciences College of Life Sciences National Yang Ming Chiao Tung University Taipei Taiwan Brain Research Center National Yang Ming Chiao Tung University Taipei Taiwan Department of Pediatrics Taipei Veterans General Hospital Taipei Taiwan Department of Biological Science and Technology National Yang Ming Chiao Tung University Hsinchu Taiwan Institute of Biology and Anatomy National Defense Medical Center Taipei Taiwan Taipei Veterans General Hospital Yuanshan and Suao Branch Yilan Taiwan Institute of Clinical Medicine National Yang Ming Chiao Tung University Taipei Taiwan

Background: We aim to characterise the ophthalmic findings and retinal vasculature changes in patients with WS, and to analyse the correlation between ophthalmic manifestations and the associated systemic diseases. Methods: This retrospective case-control study included 27 WS patients and 28 age-matched healthy participants. Stellate pattern of iris, central macular thickness (CMT), foveal width, retinal vessel diameter, superficial vascular density (SVD) of macula and foveal avascular zone (FAZ) were compared between WS patients and healthy participants. Results: Twenty-five patients (93%) had the classic stellate iris presentation. Compared with healthy controls, WS patients had decreased CMT, increased foveal width and a lower SVD of macula (all P < 0.001). Significantly decreased mean retinal arterial (117.9 ± 9.9 µm vs. 133.0 ± 6.7 µm in WS and controls, respectively;p < 0.001) and venous (158.9 ± 11.2 µm vs. 174.0 ± 8.0 µm in WS and controls, respectively;p < 0.001) outer diameters, as well as mean arterial wall thickness (11.2 ± 1.3 µm vs. 12.2 ± 0.8 µm in WS and controls, respectively;p < 0.01) were found in WS. Stellate iris grading was significantly associated with CMT, foveal width, retinal vessel diameter (all p < 0.05), and a significant increase in the odds of having hypertension (Odds ratio (OR), 5.63;P < 0.05). The severity of stellate iris in WS seemed to have the trend of increasing risk of having pulmonary stenosis, tricuspid regurgitation and mitral regurgitation. Conclusions: This study provides the first in vivo evidence reflecting current knowledge on vessel morphology in WS patients that deficient circumferential growth is the predominant pathophysiologic changes resulting from elastin deficiency. The ophthalmic characteristics may serve as a complementary tool to diagnose and follow-up patients suffering from WS. © 2022, The Author(s), under exclusive licence to The Royal College of Ophthalmologists.

关键词： Eye manifestations Predictive markers

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Incorporating animal forensics in routine meat inspection in the Philippines

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Forensic science International: Animals and Environments 2021年 1卷

作者： Fortajada, Enrykie B. Fontanilla, Ian Kendrich C. De Ungria, Maria Corazon A. Biodiversity Ethnicity and Forensics Program Philippine Genome Center University of the Philippines Philippines Science Education Institute Department of Science and Technology Taguig City Philippines DNA Barcoding Laboratory Institute of Biology University of the Philippines Diliman Philippines DNA Analysis Laboratory Natural Sciences Research Institute University of the Philippines Diliman Philippines

Robust species identification of unprocessed and processed meat is essential to ensure the safety and quality of food products. Meat adulteration results from the wrong identification of animal sources, contamination of different meats during processing, or intentional meat substitution using those from other species and non-meat products of lower economic value. This review discusses the potential applications of DNA barcoding in routine meat inspections in the Philippines. Developing mini-barcode primer sets to enhance the utility of conventional techniques is critical in adopting DNA barcoding technology as a robust tool for routine inspections of meat sold commercially, including those intended for the halal meat industry. Increasing the ability of the Philippine National Meat Inspection Service to document the number, types, and scope of meat fraud is a step forward in finally using animal forensic science as a valuable component of its regulatory functions for the protection of the meat-consuming public. © 2021 The Author(s)

关键词： Animal forensics DNA barcoding DNA mini-barcoding Meat adulteration Meat inspection Meat species identification

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Single-nucleus chromatin accessibility profiling highlights regulatory mechanisms of coronary artery disease risk (vol 54, pg 804, 2022)

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NATURE GENETICS 2022年第8期54卷 1259-1259页

作者： Turner, Adam W. Hu, Shengen Shawn Mosquera, Jose Verdezoto Ma, Wei Feng Hodonsky, Chani J. Wong, Doris Auguste, Gaelle Song, Yipei Sol-Church, Katia Farber, Emily Kundu, Soumya Kundaje, Anshul Lopez, Nicolas G. Ma, Lijiang Ghosh, Saikat Kumar B. Onengut-Gumuscu, Suna Ashley, Euan A. Quertermous, Thomas Finn, Aloke V. Leeper, Nicholas J. Kovacic, Jason C. Bjorkegren, Johan L. M. Zang, Chongzhi Miller, Clint L. Center for Public Health Genomics University of Virginia Charlottesville VA USA Department of Biochemistry and Molecular Genetics University of Virginia Charlottesville VA USA Medical Scientist Training Program University of Virginia Charlottesville VA USA Department of Pathology University of Virginia Charlottesville VA USA Robert M. Berne Cardiovascular Research Center University of Virginia Charlottesville VA USA Genome Analysis & Technology Core University of Virginia Charlottesville VA USA Genome Sciences Laboratory University of Virginia Charlottesville VA USA Department of Genetics Stanford University School of Medicine Stanford CA USA Department of Computer Science Stanford University Stanford CA USA Division of Vascular Surgery Department of Surgery Stanford University Stanford CA USA Department of Genetics and Genomic Sciences Icahn Institute for Genomics and Multiscale Biology Icahn School of Medicine at Mount Sinai New York NY USA CVPath Institute Gaithersburg MD USA Department of Public Health Sciences University of Virginia Charlottesville VA USA Division of Cardiovascular Medicine Department of Medicine Stanford University Stanford CA USA Cardiovascular Research Institute Icahn School of Medicine at Mount Sinai New York NY USA Victor Chang Cardiac Research Institute Darlinghurst New South Wales Australia St. Vincent’s Clinical School University of New South Wales Sydney New South Wales Australia Integrated Cardio Metabolic Centre Department of Medicine Karolinska Institutet Huddinge Sweden

Coronary artery disease (CAD) is a complex inflammatory disease involving genetic influences across cell types. genome-wide association studies have identified over 200 loci associated with CAD, where the majority of risk variants reside in noncoding DNA sequences impacting cis-regulatory elements. Here, we applied single-nucleus assay for transposase-accessible chromatin with sequencing to profile 28,316 nuclei across coronary artery segments from 41 patients with varying stages of CAD, which revealed 14 distinct cellular clusters. We mapped ~320,000 accessible sites across all cells, identified cell-type-specific elements and transcription factors, and prioritized functional CAD risk variants. We identified elements in smooth muscle cell transition states (for example, fibromyocytes) and functional variants predicted to alter smooth muscle cell- and macrophage-specific regulation of MRAS (3q22) and LIPA (10q23), respectively. We further nominated key driver transcription factors such as PRDM16 and TBX2. Together, this single-nucleus atlas provides a critical step towards interpreting regulatory mechanisms across the continuum of CAD risk.

关键词： Atherosclerosis Epigenomics Gene regulation

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Phage therapy

NATURE REVIEWS METHODS PRIMERS

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NATURE REVIEWS METHODS PRIMERS 2025年第1期5卷 1页

作者： [Anonymous] Human Microbiome Research Program Department of Bacteriology and Immunology Faculty of Medicine University of Helsinki Helsinki Finland European Society of Clinical Microbiology and Infectious Diseases (ESCMID) Study Group for Non-traditional Antibacterial Therapy (ESGNTA) Basel Switzerland Institute of Biomedical and Oral Research (IBOR) Faculty of Dental Medicine Hebrew University of Jerusaleum Jerusalem Israel The Israeli Phage Center (IPTC) of the Hebrew University and Hadassah Medical Center Jerusalem Israel Department of Clinical Microbiology and Infectious Diseases Hadassah–Hebrew University Medical Center Jerusalem Jerusalem Israel Laboratory of Gene Technology Department of Biosystems KU Leuven Leuven Belgium Leicester Centre for Phage Research Department of Genetics and Genome Biology University of Leicester Leicester UK Center for Evolutionary Hologenomics Globe Institute University of Copenhagen Copenhagen Denmark Centre of Excellence for Omics-Driven Computational Biodiscovery (COMBio) AIMST University Bedong Kedah Malaysia Laboratory of Phage Molecular Biology Institute of Immunology and Experimental Therapy Wrocław Poland Wrocław University of Science and Technology Faculty of Medicine Wrocław Poland Department of Biological Sciences University of Pittsburgh Pittsburgh PA USA Laboratory for Molecular and Cellular Technology Queen Astrid Military Hospital Brussels Belgium Department of Biological and Environmental Science Nanoscience Center University of Jyväskylä Jyväskylä Finland Chemical Engineering Department Loughborough University Loughborough UK

Bacteriophage (phages) are viruses that exclusively use bacterial cells for propagation, killing the bacterial host in the process. In phage therapy, phages are used to reduce bacterial numbers, thereby curing bacterial infections. Although this principle is conceptually straightforward, its practical application faces several hurdles. In this Primer, the practical aspects of phage therapy are outlined. We introduce the microbiological methods used to prepare and characterize phages and elucidate their interactions with bacteria. The discussion covers how the information in complete phage genome sequences is used, along with how RNA sequencing can enhance our understanding of phage biology. Selection parameters for therapeutic phages for clinical applications and key elements in industrial-scale phage production are provided. A summary of clinical trials both past and present, phage administration and dosing issues is analysed, as well as limitations associated with phage therapy and mitigation strategies. Finally, we speculate on the future of phage therapy.

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Synthesis and Evaluation of Metal-Impregnated Carbon Cloth Supplementation to Improve Anaerobic Digestion Performance from Municipal Sludge

SSRN

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SSRN 2023年

作者： Kumar, Vikas Devi, Parmila Patil, Abhishek Santana, Juan Camilo Bobowski, Jake S. Hallam, Steven J. Eskicioglu, Cigdem UBC Bioreactor Technology Group School of Engineering University of British Columbia Okanagan Campus KelownaBCV1V 1V7 Canada Department of Microbiology and Immunology University of British Columbia VancouverBCV6T 1Z4 Canada Department of Physics University of British Columbia Okanagan Campus KelownaBCV1V 1V7 Canada Graduate Program in Bioinformatics University of British Columbia VancouverBCV6T 1Z4 Canada Genome Science and Technology Program University of British Columbia 2329 West Mall VancouverBCV6T 1Z4 Canada Life Sciences Institute University of British Columbia VancouverBCV6T 1Z3 Canada Ecoscope Training Program University of British Columbia VancouverBCV6T 1Z3 Canada

This study investigated the effect of metal-impregnated carbon cloth (CC) supplementation on thermophilic anaerobic digestion (AD) improvement utilizing municipal sludge as a biomass input. Four types of metal-impregnated CC nanocomposites (Fe/CC, Ni/CC, Ni/Fe/CC, NiPcS/Fe/CC) were synthesized from activated CC (A-CC) using hydrothermal synthesis and compared with untreated CC (U-CC) to evaluate their functional significance for enhanced AD. The main factors inspected were electrical conductivity (EC) and Brunauer–Emmett–Teller (BET) surface area. A-CC had the highest surface area of 386.15 m2/g, followed by Fe/CC, NiPcS/Fe/CC, Ni/CC and Ni/Fe/CC with 140.96, 52.27, 43.52 and 36.07 m2/g, respectively. The A-CC and Fe/CC reactors produced 3.45 and 2.70 times more methane than Control (without CC) in the first 9 days. In contrast, the methane production for U-CC, Ni/CC, Ni/Fe/CC and NiPcS/Fe/CC was only 0.87, 1.26, 1.01 and 1.29 times, respectively, compared to Control. At a higher BET surface (>350 m2/g), the surface area becomes a more dominant factor, and the increase of EC value (>2.9*104 1/Ω.m) does not have an impact on methane enhancement. Microbial community analysis revealed that A-CC had the highest abundances of Syntrophomonas, Coprothermobacter and methanogenic populations, including Methanothermobacter and Methanosarcina, within 3 days compared to Control and metal-impregnated CC. There were minimal changes in microbial community composition in metal-impregnated CC compared to the U-CC. The result implies that the efficacy of CC supplementation for AD upgrading largely depends on the higher surface area compared to metal impregnation or conductivity. © 2023, The Authors. All rights reserved.

关键词： Anaerobic digestion

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Ultra-streamlined single cell proteomics by all-in-one chip and data-independent-acquisition mass spectrometry

Research Square

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Research Square 2021年

作者： Tu, Hsiung-Lin Gebreyesus, Sofani Tafesse Siyal, Asad Ali Kitata, Reta Birhanu Chen, Eric Sheng-Wen Enkhbayar, Bayarmaa Angata, Takashi Lin, Kuo-I Chen, Yu-Ju Institute of Chemistry Academia Sinica Taipei115 Taiwan Taiwan International Graduate program Nano Science and Technology program Academia Sinica Taipei115 Taiwan Department of Chemistry National Taiwan University Taipei106 Taiwan Taiwan International Graduate Program Chemical Biology and Molecular Biophysics Program Academia Sinica Taipei115 Taiwan Department of Chemistry National Tsing Hua University Hsinchu300 Taiwan Institute of Biological Chemistry Academia Sinica Taipei115 Taiwan Genomics Research Center Academia Sinica Taipei115 Taiwan Genome and Systems Biology Degree Program Academia Sinica and National Taiwan University Taiwan

Single cell proteomics provides the ultimate resolution to reveal cellular phenotypic heterogeneity and functional network underlying biological processes. Here, we present an ultra-streamlined workflow combining an integrated proteomic chip (iProChip) and data-independent-acquisition (DIA) mass spectrometry for sensitive microproteomics analysis down to single cell level. The iProChip offers multiplexed and automated all-in-one station from cell isolation/counting/imaging to complete proteomic processing within a single device. By mapping to project-specific spectra libraries, the iProChip-DIA enables profiling of 1160 protein groups from triplicate analysis of a single mammalian cell. Furthermore, the applicability of iProChip-DIA was demonstrated using both adherent and non-adherent malignant cells, which reveals 5 orders of proteome coverage, highly consistent ~100-fold protein quantification (1-100 cells) and high reproducibility with low missing values ( © , CC BY.

关键词： Mass spectrometry

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