检索结果-内蒙古大学图书馆

Site-Specifically modified recombinant human granulocyte colony-stimulating factor with polyethylene glycol

Biotechnology 2015年第4期14卷 200-205页

作者： Park, See-Hyoung Program in Nano Science and Technology Department of Transdisciplinary Studies Graduate School of Convergence Science and Technology Seoul National University Suwon443-270 Korea Republic of

To prepare PEGylated recombinant human granulocyte colony-stimulating factor (rHuG-CSF) with enhanced pharmacokinetic properties we prepared a cysteine-substituted mutant of rHuG-CSF (mrHuG-CSF). For site-specific PEGylation of mrHuG-CSF Threonine 134 residue that is one of the glycosylation sites and thought not to be critical in structure and function of rHuG-CSF was substituted by Cysteine. Also, Cysteine 17 residue at N-terminus that does not make di-sulfide bonds and is partially buried was substituted by Serine. This mrHuG-CSF was then site-specifically conjugated with 5 or 20 kDa of polyethylene glycol-maleimide and the correct molecular weight of the conjugates was confirmed by MALDI-TOF mass spectrometry analysis. Compared with unmodified rHuG-CSF, both PEGylated mrHuG-CSF showed similar biological activities in vitro and the plasma half-life of the 20 kDa PEGylated mrHuG-CSF was about 5-folds increased. Taken together, site-specific PEGylation of mrHuG-CSF may increase their therapeutic potency in humans. © 2015 Asian Network for Scientific Information.

关键词： Pharmacokinetics

来源：评论

学校读者我要写书评

暂无评论

Large-Scale purification of human apolipoprotein kringle domain V (rhualkv) expressed in pichia pastoris

引用

Biotechnology 2015年第4期14卷 206-210页

Human apolipoprotein kringle domain V (rHualkV) that has an anti-angiogenic activity was expressed and purified in large scale from Pichia pastoris. rHualkV was captured by SP-Streamline resin directly from 40 L culture broth through batch type adsorption with about 80% yield (n = 8). Captured rHualkV was further purified by SP-Sepharose column chromatography and concentrated by membrane filtration. On average 5 g of rHualkV was purified per batch with the 56% final yield. The result of analytical High-Performance Liquid Chromatography (HPLC) of the purified rHualkV showed the single peak. ESI mass-spectrometry analysis of the rHualkV has proved that the purified rHualkV has the 9676 kDa that is the correct molecular weight as the one calculated by amino acid sequence. In addition, rHualkV has the same N and C-terminal residue as the expected one from DNA sequence of rHualkV gene in Pichia pastoris. Thus, through three steps of process, the anti-angiogenic rHualkV was successfully expressed and purified from Pichia pastoris, which will be able to provide a basis for much larger expression and purification for production needed for clinical evaluation later. © 2015 Asian Network for Scientific Information.

关键词： Purification

来源：评论

学校读者我要写书评

暂无评论

P-149: Maximization of Transmittance and Minimization of Image-Flickering due to Flexoelectric Effect in Low-Frequency Driving Fringe-Field Switching (FFS) Mode Using LCs with Negative Dielectric Anisotropy

引用

SID Symposium Digest of Technical Papers 2017年第1期48卷

作者： Chang Suk Lee Han Sol Choi Hyeong Gyun Ham Hye Sun Yoo MinSu Kim Young Jin Lim Tae Hyeong Kim Philip J. Bos Deng-Ke Yang Seung Hee Lee Department of BIN Convergence Technology and Department of Polymer Nano-Science and Technology Chonbuk National University Jeonju Jeonbuk 54896 Korea Department of Physics and Astronomy Johns Hopkins University Baltimore MD 21218 USA Graduate School of Flexible and Printable Electronics Engineering Chonbuk National University Jeonju Jeonbuk 54896 Korea Liquid Crystal Institute Kent State University Kent OH 44242 United States

Image-flickering due to flexoelectric effect in a low frequency driven fringe-field switching (FFS) LCD is troublesome. Maximization of transmittance in the FFS mode is achieved using LCs with negative dielectric anisotropy but still the flickering exists. The paper describes in detail on optimal solutions how it can be solved by controlling the ratio of electrode width to distance and thickness of passivation layer. The results greatly contribute to achieve high transmittance and image-flickering free FFS LCD.

关键词： FFS mode image-flicker flexoelectric effect negative liquid crystal fine-patterned electrode

来源：评论

学校读者我要写书评

暂无评论

Great influence of inorganic salts for controllable synthesis of YF3:Yb3+/Er3+ nanocrystals 22

Great influence of inorganic salts for controllable synthesi...

引用

22nd International Display Workshops, IDW 2015

作者： Murali, G. Mishra, R.K. Lee, Jae Myeong Lee, Byeong Hoon Ham, Hyuong Gyun Kim, Jin Hyun Lim, Dong-Kwon Lee, Seung Hee Applied Materials Institute for BIN Convergence Department of BIN Fusion Technology Department of Polymer-Nano Science and Technology Chonbuk National University Jeonju Jeonbuk561-756 Korea Republic of KU-KIST Graduate School of Converging Science and Technology Korea University 145 Anam-ro Seongbuk-gu Seoul136-701 Korea Republic of

ISBN: (纸本)9781510845503

Shape controllable synthesis of YF3:Yb3+/Er3+ upconversion nanoparticles is of significant importance for understanding the shape dependent properties as well as for exploration of potential applications. Herein, YF3:Yb/Er nanorods and nanoneedles were selectively synthesized using CaCl2.2H2O and KNO3 inorganic salts, respectively. © Proceedings of the International Display Workshops. All rights reserved.

关键词： nanorods

来源：评论

学校读者我要写书评

暂无评论

Materialization of strained CVD-graphene using thermal mismatch

引用

nano Research 2015年第6期8卷 2082-2091页

作者： Seung-Mo Lee Sang-Min Kim Min Young Na Hye Jung Chang Kwang-Seop Kim Hyunung Yu Hak-Joo Lee Jae-Hyun Kim Nano-Convergence Mechanical Systems Research Division Korea Institute of Machinery & Materials (KIMM) 156 Gajungbuk-ro Yuseong-gu Daejeon 305-343 Republic of Korea Nano Mechatronics University of Science and Technology (UST) 217 Gajeong-ro Yuseong-gu Daejeon 305-333 Republic of Korea Graduate School of Energy Environment Water and Sustainability Korea Advanced Institute of Science & Technology (KAIST) Daejeon 305-701 Republic of Korea Department of Materials Science and Engineering Yonsei University SO Yonsei-ro Seodaemun-gu Seou1120-749 Republic of Korea Advanced Analysis Center Korea Institute of Science and Technology (KIST) Hwarang-ro 14-gil 5 Seongbuk-gu Seoul 130-791 Republic of Korea Korea Research Institute of Standards and Science (KRISS) 267 Gajeong-ro Yuseong-gu Daejeon 305-343 Republic of Korea

Theoretical physics foretells that ＂strain engineering＂ of graphene could hold the key to finding treasures still hidden in two-dimensional （2D） condensed matter physics and commercializing graphene-based devices. However, to produce strained graphene in large quantities is not an easy task by any means. Here, we demonstrate that thermal annealing of graphene placed on various substrates could be a surprisingly simple method for preparing strained graphene with a large area. We found that enhanced graphene-substrate interfacial adhesion plays a critical role in developing strained graphene. Creative device architectures that consider the thermal mismatch between graphene and the target substrate could enable the resulting strain to be intentionally tailored. We believe that our proposed method could suggest a shortcut to realization of graphene straintronics.

关键词： graphene strain engineering thermal mismatch strained graphene

来源：评论

学校读者我要写书评

暂无评论

A keyboard-based r-shaped triboelectric generator for active noise-free recording

A keyboard-based r-shaped triboelectric generator for active...

引用

2015 MRS Spring Meeting

作者： Han, Mengdi Meng, Bo Cheng, Xiaoliang Zhu, Fuyun Shi, Mayue Zhang, Haixia National Key Laboratory of Science Technology on Micro/Nano Fabrication Peking University Beijing100871 China Peking University Shenzhen Graduate School Shenzhen518055 China

ISBN: (纸本)9781510826373

This paper presents a method for noise-free recording of the keyboard-based musical instruments. By integrating an r-shaped triboelectric generator (TEG) into the keyboard of a piano, it can produce electric signal while playing music due to the combination of contact electrification and electric induction. We investigated the electric signal and developed a graphical user interface to convert the electric signal back to music. Using the piano and the graphical user interface, active noise-free recording can be realized. Namely, only the sound produced by the piano can be converted and recorded, while other noise in the environment can be filtered automatically. © 2015 Materials Research Society.

关键词： Music

来源：评论

学校读者我要写书评

暂无评论

26th Annual Computational Neuroscience Meeting (CNS*2017) of the Organization for Computational Neuroscience Antwerp, Belgium, July 15-20, 2017

引用

BMC NEUROscience 2017年第SUPPL 1期18卷 59-59页

作者： [Anonymous] Indiana University Purdue University Indianapolis Indianapolis IN 46032 USA Stark Neurosciences Research Institute Indiana University School of Medicine Indianapolis IN 46032 USA Department of Mathematics East Carolina University Greenville NC 27858 USA Jülich Supercomputing Centre Forschungszentrum Jülich 52425 Jülich Germany Future Systems Swiss National Supercomputing Centre 8092 Zurich Switzerland User Engagement and Support Swiss National Supercomputing Centre 6900 Lugano Switzerland Institut de Neurosciences des Systèmes Aix Marseille Univ 13005 Marseille France Simulation Lab Neuroscience Forschungszentrum Jülich Jülich Germany Department of Experimental Psychology Ghent University 9000 Ghent Belgium Donders Center for Cognitive Neuroimaging Radboud University 6525HR Nijmegen The Netherlands Department of Electrical Computer and Energy Engineering University of Colorado Boulder CO 80309 USA Department of Neurosurgery Johns Hopkins School of Medicine Baltimore MD 21287 USA Department of Neurology Johns Hopkins School of Medicine Baltimore MD 21287 USA Department of Otolaryngology Johns Hopkins School of Medicine Baltimore MD 21287 USA INSERM U968 Paris France Sorbonne Universités UPMC University Paris 06 UMR_S 968 Institut de la Vision Paris France CNRS UMR_7210 Paris France Department of Computer Architecture and Technology University of Granada (CITIC) Granada Spain Sorbonne Universités UPMC Univ Paris 06 INSERM CNRS Institut de la Vision Paris France Department of Adaptive Machine Systems Osaka University Osaka Japan Department of Computer Science University of Cergy-Pontoise Cergy-Pontoise France Department of Physics and Astronomy College of Charleston Charleston SC 29424 USA School of Physics Faculty of Science University of Sydney Sydney NSW 2006 Australia Center of Excellence for Integrative Brain Function Australian Research Council Sydney Australia Max Planck Institute for Human Cognitive and Brain Sciences Saxony Lei

来源：评论

学校读者我要写书评

暂无评论

Electromechanical properties of printed copper ink film using a white flash light annealing process for flexible electronics

Electromechanical properties of printed copper ink film usin...

引用

作者： Eun, Kyoungtae Chon, Min-Woo Yoo, Tae-Hee Song, Yong-Won Choa, Sung-Hoon Graduate School of NID Fusion Technology Seoul National University of Science and Technology Seoul139-743 Korea Republic of Department of Mechanics and Design Kookmin University Jeongneung-Ro 77 Seongbuk-Gu Seoul136-702 Korea Republic of Future Convergence Research Division Korea Institute of Science and Technology Seoul136-791 Korea Republic of

We report on a systematic study of the electromechanical properties of flexible copper (Cu) thin film for flexible electronics. Cu ink is synthesized with chemical reduction process. Cu ink film spin-coated on a polyimide substrate is annealed with white flash light, also known as intense pulsed light (IPL), which guarantees a room temperature and sub-second process in ambient conditions. IPL annealed Cu film shows the electrical resistivity of 4.8 μΩ cm and thickness of 200 nm. The electromechanical properties of IPL annealed Cu film are investigated via outer/inner bending, stretching, and adhesion tests, and it is compared with conventional electron-beam evaporated Cu film. IPL annealed Cu film shows a constant electrical resistance within a bending radius of 6 mm. The bending fatigue test shows that the Cu film can withstand 10,000 bending cycles. In the stretching test, the Cu film shows a 50% increase in resistance when a strain of 2.4% was induced. At 4% strain, the resistance increases more than 200%. Meanwhile, the electron-beam evaporated film shows a constant resistance up to a strain of 4%. Lower stretchability of IPL annealed Cu film is attributed to its inherent cracks and porous film morphologies. IPL annealing induces the local melting at the interface between the substrate and Cu film, which increases the adhesion strength of the Cu film. These results provide useful information regarding the mechanical flexibility and durability of the nanoparticle films for the development of flexible electronics. © 2015 Elsevier Ltd.

关键词： Bending tests

来源：评论

学校读者我要写书评

暂无评论

Recent progress of M13 virus-based chemical and biological sensing

引用

Toxicology and Environmental Health sciences 2015年第5期7卷 251-261页

作者： Moon, Jong-Sik Kim, Chuntae Kim, Won-Geun Han, Jiye Sohn, Jong-Ryeul Oh, Jin-Woo BK21 Plus Division of Nano Convergence Technology Pusan National University Busan 46241 South Korea Department of Nano Fusion Technology Pusan National University Busan 46241 South Korea BK21 PLUS Program in Embodiment: Health-Society Interaction Department of Public Health Sciences Graduate School Korea University Seoul 02708 South Korea Department of Nanoenergy Engineering Pusan National University Busan 46241 South Korea

Discriminating the minute content of chemicals both in precise and concise way is to use core technique for detecting water pollution. Recently a novel virus-based sensor system functionalized by M13 bacte-riophage-based structure got great attention. This system can detect various chemicals in superior sensitivity and selectivity. The filamentous and consistent shape of M13 bacteriophage can be ordered by self-assembly technique in high established form. This allows M13 bacteriophage as a template to build homogeneous distribution and permeable network structures of inorganic nanostructures under mild conditions. Phage display, genetic engineering technique of M13 bacteriophage, is another strong feature of M13 bacteriophage as a functional building block. The numerous possibility of genetic modification of M13 bacteriophage is definitely a key feature, and we have seen only the tip of an iceberg of it so far. Here, we review the very recent progress in the application of M13 bacteriophage self-assembly structures to a sensor system and discuss about M13 bacteriophage technology of our future. © 2015, Korean Society of Environmental Risk Assessment and Health science and Springer science+Business Media Dordrecht.

关键词： Biocompatibility Genetic engineering M13 bacteriophage Self-assembly

来源：评论

学校读者我要写书评

暂无评论

P-100: Fast Response and Scattering Free Optically Isotropic Liquid Crystal Device for Flexible Display Applications

引用

SID Symposium Digest of Technical Papers 2016年第1期47卷

作者： Chul Ho Park Eun Jeong Shin Ramesh Manda Seong Cheol Noh Myoung-Hoon Lee Seung Hee Lee Applied Materials Institute for BIN Convergence Department of BIN Convergence Technology and Department of Polymer Nano Science and Technology Chonbuk National University Jeonju Jeonbuk 561-756 Korea The Graduate School of Flexible and Printable Electronics Chonbuk National University Jeonju Jeonbuk 561-756 Korea

We demonstrated a novel optically isotropic liquid crystal (OILC) mixture with acrylate type of functional group monomers. By controlling the ratio of LC to monomer ad UV intensity, an excellent transparent OILC composite is obtained and the inplane field driven device exhibits a higher transmittance by 49% and a faster response time by 40% than those of OILC with thiolene based monomers. This high level black state and its flexibility can be applicable to flexible TFT-LCDs.

关键词： Optically Isotropic Liquid Crystal Monomer High Transmittance Flexible LCD

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：