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Biomimetics: Sulfated Alginate as a Mimic of Sulfated Glycosaminoglycans: Binding of Growth Factors and Effect on Stem Cell Behavior (Adv. Biosys. 7/2017)

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Advanced Biosystems 2017年第7期1卷

作者： Rami Mhanna Jana Becher Matthias Schnabelrauch Rui L. Reis Iva Pashkuleva 3B's Research Group—Biomaterials Biodegradables and Biomimetics University of Minho Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine AvePark 4806–909 Taipas Guimarães Portugal ICVS/3B's PT Government Associate Laboratory Braga/Guimarães Portugal Biomedical Engineering and Chemical Engineering Program American University of Beirut Beirut 1107 2020 Lebanon INNOVENT e.V. Biomaterials Department Prüssingstraße 27 B D-07745 Jena Germany

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Abstracts from the 20th International Symposium on Signal Transduction at the Blood-Brain Barriers Abstracts

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FLUIDS AND BARRIERS OF THE CNS 2017年第SUPPL 2期14卷 27-27页

作者： [Anonymous] The Jerzy Kukuczka Academy of Physical Education Katowice Poland The Institute of Animal Physiology and Food Research Polish Academy of Sciences Olsztyn Poland University of Miami Miami FL USA Institute of Pharmaceutical Science King’s College London London United Kingdom Department of Ophthalmology and Molecular and Integrative Physiology The University of Michigan Ann Arbor MI USA Department of Internal Medicine and Cardiology The University of Michigan Ann Arbor MI USA School of Medicine Department of Cellular and Molecular Medicine Koc University Istanbul Turkey Department of Cellular and Molecular Medicine Medical Faculty Koc University Istanbul Turkey Department of Neuroscience School of Medicine Koc University Istanbul Turkey Department of Neuroscience Medical Faculty Koc University Istanbul Turkey Department of Laboratory Animals Science Aziz Sancar Experimental Medicine Research Institute Istanbul University Istanbul Turkey Department of Neuroscience Aziz Sancar Experimental Medicine Research Institute Istanbul University Istanbul Turkey School of Medicine Koc University Istanbul Turkey Department of Forensic Science Istanbul Faculty of Medicine Istanbul University Istanbul Turkey Department of Histology and Embryology School of Medicine Koc University Istanbul Turkey Department of Histology and Embryology Istanbul Faculty of Medicine Istanbul University Istanbul Turkey Department of Physiology School of Medicine Koc University Istanbul Turkey Theodor Kocher Institute University of Bern Bern Switzerland Institute of Veterinary Physiology Vetsuisse Faculty University of Zurich Zurich Switzerland Zurich Center for Integrative Human Physiology University of Zurich Zurich Switzerland Zurich Center of Integrative Human Physiology University of Zurich Zurich Switzerland Institute for Veterinary Physiology and Zurich Center for Integrative Human Physiology University of Zurich Zurich Switzerland Institute of Veterinary Physiology Univer

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Cadaveric bone marrow mesenchymal stem cells:first experience treating a patient with large severe burns

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Burns & Trauma 2015年第2期3卷 74-82页

作者： Eduardo Mansilla Gustavo H.Marín Mirta Berges Silvia Scafatti Jaime Rivas Andrea Núñez Martin Menvielle Roberto Lamonega Cecilia Gardiner Hugo Drago Flavio Sturla Mercedes Portas Silvia Bossi Maria Victoria Castuma Sandra Peña Luengas Gustavo Roque Karina Martire Jose Maria Tau Gabriel Orlandi Adrian Tarditti Tissue Engineering Regenerative Medicine and Cell Therapies LaboratoryCUCAIBAProvince of Buenos Aires Ministry of HealthTransplantation ProgramLa PlataProvince of Buenos AiresArgentina Burns and Plastic Surgery Department San Martín HospitalProvince of Buenos Aires Ministry of HealthLa PlataProvince of Buenos AiresArgentina Burns Hospital Buenos Aires CityArgentina Department of Chemistry Mayaguez CampusUniversity of Puerto RicoMayaguezPuerto RicoUSA.

Background:In January 2005,Rasulov et *** published"First experience in the use of bone marrow mesenchymal stem cells(MSCs)for the treatment of a patient with deep skin burns".Here,we present the first ever treated patient with cadaveric bone marrow mesenchymal stem cells(CMSCs)in the history of ***:A young man,who severely burned 60%of his total body surface with 30%of full-thickness burns while working with a grass trimmer that exploded,was involved in the *** were obtained from the bone marrow of a cadaver donor in a routine procurement procedure of CUCAIBA,the Province of Buenos Aires,Argentina,Ministry of Health,Transplantation Agency,cultured,expanded,and applied on the burned surfaces using a fibrin spray after early ***:So far,our preliminary experience and our early results have been very impressive showing an outstanding safety data as well as some impressive good results in the use of ***:Based on al this,we think that improvements in the use of stem cells for burns might be possible in the near future and a lot of time as well as many lives could be saved by many other research teams all over the *** will probably be a real scientific opportunity in regenerative medicine as well as in Transplantation.

关键词： Burns Cadaveric Transplantation Skin regeneration Mesenchymal stem cells

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Abstracts from the 8th International Conference on cGMP Generators, Effectors and Therapeutic Implications : Bamberg, Germany. 23-25 June, 2017

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BMC pharmacology & toxicology 2017年第SUPPL 1期18卷 64-64页

作者： Todd Milne, G. Sandner, Peter Lincoln, Kathleen A. Harrison, Paul C. Chen, Hongxing Wang, Hong Clifford, Holly Qian, Hu Sheng Wong, Diane Sarko, Chris Fryer, Ryan Richman, Jeremy Reinhart, Glenn A. Boustany, Carine M. Pullen, Steven S. Andresen, Henriette Moltzau, Lise Román Cataliotti, Alessandro Levy, Finn Olav Lukowski, Robert Frankenreiter, Sandra Friebe, Andreas Calamaras, Timothy Baumgartner, Robert McLaughlin, Angela Aronovitz, Mark Baur, Wendy Wang, Guang-Rong Kapur, Navin Karas, Richard Blanton, Robert Hell, Stefan Waldman, Scott A. Lin, Jieru E. Colon-Gonzalez, Francheska Kim, Gilbert W. Blomain, Erik S. Merlino, Dante Snook, Adam Erdmann, Jeanette Wobst, Jana Kessler, Thorsten Schunkert, Heribert Walter, Ulrich Pagel, Oliver Walter, Elena Gambaryan, Stepan Smolenski, Albert Jurk, Kerstin Zahedi, Rene Klinger, James R. Benza, Raymond L. Corris, Paul A. Langleben, David Naeije, Robert Simonneau, Gérald Meier, Christian Colorado, Pablo Chang, Mi Kyung Busse, Dennis Hoeper, Marius M. Masferrer, Jaime L. Jacobson, Sarah Liu, Guang Sarno, Renee Bernier, Sylvie Zhang, Ping Flores-Costa, Roger Currie, Mark Hall, Katherine Möhrle, Dorit Reimann, Katrin Wolter, Steffen Wolters, Markus Mergia, Evanthia Eichert, Nicole Geisler, Hyun-Soon Ruth, Peter Feil, Robert Zimmermann, Ulrike Koesling, Doris Knipper, Marlies Rüttiger, Lukas Tanaka, Yasutake Okamoto, Atsuko Nojiri, Takashi Kumazoe, Motofumi Tokudome, Takeshi Miura, Koichi Hino, Jun Hosoda, Hiroshi Miyazato, Mikiya Kangawa, Kenji Kapil, Vikas Ahluwalia, Amrita Paolocci, Nazareno Eaton, Philip Campbell, James C. Henning, Philipp Franz, Eugen Sankaran, Banumathi Herberg, Friedrich W. Kim, Choel Wittwer, M. Luo, Q. Kaila, V. Dames, S. A. Tobin, Andrew Alam, Mahmood Rudyk, Olena Krasemann, Susanne Hartmann, Kristin Prysyazhna, Oleksandra Zhang, Min Zhao, Lan Weiss, Astrid Schermuly, Ralph Moyes, Amie J. Chu, Sandy M. Baliga, Reshma S. Hobbs, Adrian J. Michalakis, Stylianos Mühlfriedel, Regine Schön, Christian Fischer, Dominik M. Wilhelm, Barbara Zobor, Ditta Kohl, Ironwood Pharmaceuticals Cambridge USA Bayer AG Drug Discovery - Cardiovascular Research Wuppertal Germany Department of Cardiometabolic Diseases Research Boehringer Ingelheim Pharmaceuticals Ridgefield USA Department of Small Molecule Drug Research Boehringer Ingelheim Pharmaceuticals Ridgefield USA Department of Pharmacology University of Oslo and Oslo University Hospital Oslo Norway Institute of Experimental Medical Research University of Oslo and Oslo University Hospital Oslo Norway Center for Heart Failure Research University of Oslo and Oslo University Hospital Oslo Norway Department of Pharmacology Toxicology and Clinical Pharmacy Institute of Pharmacy University of Tuebingen Tuebingen Germany Physiologisches Institut Universität Würzburg Würzburg Germany Molecular Cardiology Research Institute Tufts Medical Center Boston USA Present Affiliation: Division of Cardiology University of Pittsburgh Medical Center Pittsburgh USA Present Affiliation: Department of Medicine Brown University School of Medicine Providence USA Division of Cardiology Tufts Medical Center Boston USA Max Planck Institute for Biophysical Chemistry Göttingen and German Cancer Research Center (DKFZ) Heidelberg Germany Department of Pharmacology and Experimental Therapeutics Thomas Jefferson University Philadelphia USA Institut für Kardiogenetik and Universitäres Herzzentrum Lübeck Universität zu Lübeck Lübeck Germany & DZHK e.V. (German Center for Cardiovascular Research) Partner Site Hamburg/Kiel/Lübeck Lübeck Germany Deutsches Herzzentrum München Klinik für Herz- und Kreislauferkrankungen Technische Universität München Munich Germany Center for Thrombosis & Hemostasis Universitätsklinikum der Johannes Gutenberg-Universität Mainz Mainz Germany Leibniz-Institut für Analytische Wissenschaften – ISAS – e.V Dortmund Germany Sechenov Institute of Evolutionary Physiology and Biochemistry Russian Academy of Sciences St. Petersburg Russia UCD Conway Institute UCD School of Medicine

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A cost effective and highly sensitive glucose biosensor based on a 3D silicon nano wire array electrode

A cost effective and highly sensitive glucose biosensor base...

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Design, Test, Integration and Packaging of MEMS/MOEMS

作者： Wen-Chao Feng Che-Wei Hsu Hsi-Chien Liu Gou-Jen Wang Department of Mechanical Engineering National Chung-Hsing University Taichung Taiwan PhD Program in Tissue Engineering and Regenerative Medicine National Chung-Hsing University Taichung Taiwan

In this study, a novel glucose biosensor based on a 3D silicon nanowire array (SNA) electrode was proposed. Metal-assisted etching (MAE) method using an AgNO 3 and HF mixing solution as the etchant is employed for fast etching of a silicon wafer to form the SNA electrode. A thin gold shell is then coated onto each silicon nanowire by sputtering. Potassium ferricyanide, glucose oxidase, and a Nafion thin film were then sequentially coated onto the fabricated 3D SNA for glucose detection. The effective sensing area of the fabricated 3D SNA electrode was measured to be 11.35 folds that of the corresponding plane electrode by steady-state voltammetry. Actual glucose detections illustrated that the SNA based devices could function at a sensitivity of 1,034 μA mM -1 cm -2 with a linear detection range from 55.1 μM-11.0 mM and detection limit of 11 μM. A fast response time of 2 s was also demonstrated. The proposed 3D SNA based glucose biosensing scheme possesses advantages of low cost, high sensitivity, and fast response.

关键词： Sugar Electrodes Biosensors Gold Silicon Three-dimensional displays

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Corrigendum to "The Overexpression of NALP3 Inflammasome in Knee Osteoarthritis Is Associated with Synovial Membrane Prolidase and NADPH Oxidase 2"

Oxidative medicine and cellular longevity

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Oxidative medicine and cellular longevity 2017年第1期2017卷 7847602页

作者： Denise Clavijo-Cornejo Karina Martínez-Flores Karina Silva-Luna Gabriela Angélica Martínez-Nava Javier Fernández-Torres Yessica Zamudio-Cuevas Mónica Guadalupe Santamaría-Olmedo Julio Granados-Montiel Carlos Pineda Alberto López-Reyes Synovioanalysis Molecular Laboratory Instituto Nacional de Rehabilitación "Luis Guillermo Ibarra Ibarra" Secretaria de Salud Calzada Mexico-Xochimilco No. 289 Col. Arenal de Guadalupe 14389 Tlalpan Mexico City Mexico. Musculoskeletal and Articular Ultrasound Diploma Course Instituto Nacional de Rehabilitación "Luis Guillermo Ibarra Ibarra" Secretaria de Salud Calzada Mexico-Xochimilco No. 289 Col. Arenal de Guadalupe 14389 Tlalpan Mexico City Mexico. Synovioanalysis Molecular Laboratory Instituto Nacional de Rehabilitación "Luis Guillermo Ibarra Ibarra" Secretaria de Salud Calzada Mexico-Xochimilco No. 289 Col. Arenal de Guadalupe 14389 Tlalpan Mexico City Mexico Biological and Health Sciences PhD Program Universidad Autónoma Metropolitana Iztapalapa Avenida San Rafael Atlixco 186 Col. Vicentina 09340 Iztapalapa Mexico City Mexico. Tissue Engineering Cell Therapy and Regenerative Medicine Research Unit Instituto Nacional de Rehabilitación "Luis Guillermo Ibarra Ibarra" Secretaria de Salud Calzada Mexico-Xochimilco No. 289 Col. Arenal de Guadalupe 14389 Tlalpan Mexico City Mexico. Instituto Nacional de Rehabilitación "Luis Guillermo Ibarra Ibarra" Secretaria de Salud Calzada Mexico-Xochimilco 289 Col. Arenal de Guadalupe 14389 Tlalpan Mexico City Mexico.

[This corrects the article DOI: 10.1155/2016/1472567.].

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Induced Pluripotent Stem Cell-derived Mesenchymal Stem Cell Seeding on Biofunctionalized Calcium Phosphate Cements

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Bone Research 2013年第4期1卷 371-384页

作者： WahWah TheinHan Jun Liu Minghui Tang Wenchuan Chen Linzhao Cheng Hockin H.K.Xu Biomaterials & Tissue Engineering Division Department of EndodonticsProsthodontics and Operative DentistryUniversity of Maryland Dental School State Key Laboratory of Oral Diseases West China Hospital of StomatologySichuan University Stem Cell Program in Institute for Cell Engineering and Division of Hematology Johns Hopkins University Center for Stem Cell Biology & Regenerative Medicine University of Maryland School of Medicine University of Maryland Marlene and Stewart Greenebaum Cancer Center University of Maryland School of Medicine Department of Mechanical Engineering University of Maryland

Induced pluripotent stem ceils （iPSCs） have great potential due to their proliferation and differentiation capability. The objectives of this study were to generate iPSC-derived mesenchymal stem cells （iPSC-MSCs）, and investigate iPSC-MSC proliferation and osteogenic differentiation on calcium phosphate cement （CPC） containing biofunctional agents for the first time. Human iPSCs were derived from marrow CD34＋ cells which were reprogrammed by a single episomal vector, iPSCs were cultured to form embryoid bodies （EBs）, and MSCs migrated out of EBs. Five biofunctional agents were incorporated into CPC： RGD （Arg-Gly-Asp） peptides, fibronectin （Fn）, fibronectin-like engineered polymer protein （FEPP）, extracellular matrix Geltrex, and platelet concentrate, iPSC-MSCs were seeded on five biofunctionalized CPCs： CPC-RGD, CPC-Fn, CPC- FEPP, CPC-Geltrex, and CPC-Platelets. iPSC-MSCs on biofunctional CPCs had enhanced proliferation, actin fiber expression, osteogenic differentiation and mineralization, compared to control. Cell proliferation was greatly increased on biofunctional CPCs. iPSC-MSCs underwent osteogenic differentiation with increased alkaline phosphatase, Runx2 and coUagen-I expressions. Mineral synthesis by iPSC-MSCs on CPC-Platelets was 3-fold that of CPC control. In conclusion, iPSCs showed high potential for bone engineering, iPSC- MSCs on biofunctionalized CPCs had cell proliferation and bone mineralization that were much better than traditional CPC. iPSC-MSC-CPC constructs are promising to promote bone regeneration in craniofacial/ orthopedic repairs.

关键词： induced pluripotent stem cells RGD fibronectin platelet concentrate biofunctionalized calciumphosphate cement bone tissue engineering

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Reduction of incubation time and enhancement of analyte adhesion uniformity of impedance biosensors using microvibration method

Reduction of incubation time and enhancement of analyte adhe...

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第八届全国微全分析系统学术会议、第三届全国微纳尺度生物分离分析学术会议暨第五届国际微化学与微系统学术会议

作者： Jaw-Ji Tsai Yi-Fen Liu En-Chih Liao Jau-Liang Chen Gou-Jen Wang Department of Mechanical Engineering National Chung-Hsing UniversityTaichungTaiwan Ph.D. Program in Tissue Engineering and Regenerative Medicine National Chung-Hsing UniversityTaichungTaiwan Department of Medical Education & Research Taichung Veterans General HospitalTaichungTaiwan Graduate Institute of Biomedical EngineeringNational Chung-Hsing UniversityTaichungTaiwan Department of Mechanical EngineeringNational Chung-Hsing UniversityTaichungTaiwan Graduate Institute of Biomedical EngineeringNational Chung-Hsing UniversityTaichungTaiwan

Electrochemical impedance spectroscopy (EIS) analysis can detect an analyte with high sensitivity in short *** self-assembled monolayer (SAM) technique is usually used for attaching analytes to a *** general,the relatively time-consuming incubation time and the non-uniform immobilization of an analyte on the sensing electrode are the commonly encountered *** study proposes a simple microvibration method based on Bernoulli's theory for improving incubation time and enhancing the adhesion uniformity of the *** detection of the group-2 allergen Der p2 demonstrates that a suitable applied frequency at a fixed amplitude can considerably reduce the sample preparation time and improve the adhesion and adhesive uniformity of the *** is also found that compared to the conventional dipping method the microvibration method can give a relatively large detection range,provide a relatively better detection linearity,and result in a standard detection curve with a relatively large slope so that the sensitivity of the sensor can be increased and the detection error due to measurement variations can be *** proposed scheme can be further applied to other detection methods that use surface-bound reactions for analyte immobilization.

关键词： Electrochemical impedance spectroscopy analysis Self-assembled monolayer immobilization Microvibration Bernoulli' s theory

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Induced pluripotent stem cell-derived mesenchymal stem cell seeding on biofunctionalized calcium phosphate cements

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Bone research [Bone Res] 2013年 4卷 371-384页

作者： TheinHan Liu Tang Chen Cheng Xu H Biomaterials & Tissue Engineering Division Department of Endodontics Prosthodontics and Operative Dentistry University of Maryland Dental School Baltimore MD 21201 USA State Key Laboratory of Oral Diseases West China Hospital of Stomatology Sichuan University Chengdu 610041 China Stem Cell Program in Institute for Cell Engineering and Division of Hematology Johns Hopkins University Baltimore MD 21205 USA Center for Stem Cell Biology & Regenerative Medicine University of Maryland School of Medicine Baltimore MD 21201 USA University of Maryland Marlene and Stewart Greenebaum Cancer Center University of Maryland School of Medicine Baltimore MD 21201 USA Department of Mechanical Engineering University of Maryland Baltimore County MD 21250 USA

Induced pluripotent stem cells (iPSCs) have great potential due to their proliferation and differentiation capability. The objectives of this study were to generate iPSC-derived mesenchymal stem cells (iPSC-MSCs), and investigate iPSC-MSC proliferati on and osteogenic differentiation on calcium phosphate cement (CPC) containing biofunctional agents for the first time. Human iPSCs were derived from marrow CD34+ cells which were reprogrammed by a single episomal vector. iPSCs were cultured to form embryoid bodies (EBs), and MSCs migrated out of EBs. Five biofunctional agents were incorporated into CPC: RGD (Arg-Gly-Asp) peptides, fibronectin (Fn), fibronectin-like engineered polymer protein (FEPP), extracellular matrix Geltrex, and platelet concentrate. iPSC-MSCs were seeded on five biofunctionalized CPCs: CPC-RGD, CPC-Fn, CPC-FEPP, CPC-Geltrex, and CPC-Platelets. iPSC-MSCs on biofunctional CPCs had enhanced proliferation, actin fiber expression, osteogenic differentiation and mineralization, compared to control. Cell proliferation was greatly increased on biofunctional CPCs. iPSC-MSCs underwent osteogenic differentiation with increased alkaline phosphatase, Runx2 and collagen-I expressions. Mineral synthesis by iPSC-MSCs on CPC-Platelets was 3-fold that of CPC control. In conclusion, iPSCs showed high potential for bone engineering. iPSC-MSCs on biofunctionalized CPCs had cell proliferation and bone mineralization that were much better than traditional CPC. iPSC-MSC-CPC constructs are promising to promote bone regeneration in craniofacial/orthopedic repairs.

关键词： RGD biofunctionalized calcium phosphate cement bone tissue engineering fibronectin induced pluripotent stem cells platelet concentrate

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Operative Debridement of Diabetic Foot Ulcers

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Journal of the American College of Surgeons 2008年第6期207卷 e1-e6页

作者： Golinko, Michael S. Joffe, Renta Maggi, Jason Cox, Dalton Chandrasekaran, Eashwar B. Tomic-Canic, R. Marjana Brem, Harold Department of Surgery Division of Wound Healing and Regenerative Medicine New York University School of Medicine New York NY United States Department of Pathology Columbia College of Physicians and Surgeons New York NY United States Hospital for Special Surgery Tissue Engineering Repair and Regeneration Program New York NY United States

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