In this contribution, the characteristics and anticancer activity of chromium (Cr) doped Fe3O4 nanoparticles, of the form Fe2+(Fe3+0.95Cr3+0.05)2O4, with 0 3O4 nanoparticles were synthesized using the green synthesis ...
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Glioblastoma (GBM), a cancer type, overexpresses heat shock protein 27 (HSP27), a member of heat shock proteins (HSPs) that activates the nuclear androgen receptor's (AR) transcriptional activity. Blocking AR sign...
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Empirical structural methods have been instrumental in drug design, but they often fall short of capturing protein dynamics. To address this limitation, computer-aided drug design (CADD) is essential. Given that Schis...
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Heterologous expression of exogenous proteases in Escherichia coli often results in the formation of insoluble inclusion bodies. When sequestered into inclusion bodies, the functionality of the proteases is minimized....
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In this dispensation of the fourth industrial revolution,protein engineering has become a popular approach for increasing enzymatic activity,stability,and titer in the biosynthesis of natural *** is attributed to its ...
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In this dispensation of the fourth industrial revolution,protein engineering has become a popular approach for increasing enzymatic activity,stability,and titer in the biosynthesis of natural *** is attributed to its numerous advantages(over direct isolation from plants or via chemical synthesis),including decreasing or eliminating reaction byproducts,high precision,moderate handling of intricate and chemically unstable chemicals,overall reusability,and cost ***,protein engineering tools have advanced to redesign and enhance natural product *** methods include direct evolution,substrate engineering,medium engineering,enzyme engineering and immobilization,structure-assisted protein engineering,and advanced *** successes in implementing these emerging protein engineering technologies were critically discussed in this ***,the advantages,limitations,and applications in industrial and medical biotechnology were ***,future research directions and potential were also highlighted.
This study aimed to determine whether C. mannii seed oil (CMSO) could shield rats from cyclophosphamide-induced hepatorenal toxicity. The focus was on its antioxidant and anti-inflammatory properties. We randomly assi...
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This study aimed to determine whether C. mannii seed oil (CMSO) could shield rats from cyclophosphamide-induced hepatorenal toxicity. The focus was on its antioxidant and anti-inflammatory properties. We randomly assigned thirty-six (36) male Wistar rats into six groups of six rats each. Groups A and B served as normal and negative controls, respectively. Group C, the standard control, was administered 300 mg/kg body weight (bw) of Omega 3 oil for 27 days, followed by 100 mg/kg bw of cyclophosphamide on day 28. Group D, E, and F received 5, 2.5, and 1.5 ml/kg b.w. of CMSO for 27 days, then 100 ml/kg b.w. of cyclophosphamide on day 28. We measured the body weights of the experimental rats every week. Rats of all groups were sacrificed on day 30 and collected blood for biochemical analysis using standard methods. The phytochemical constituents were determined by the spectrophotometric method. The phytochemical study of CMSO indicated the relative composition of constituents(mg/100g) as phenols (30 %), tannins (20 %), flavonoids (18 %), terpenoids (15 %), glycosides (10 %), alkaloids (5 %), and HCN (2 %) in Cucumeropsis mannii seed oil. When cyclophosphamide was given to Wistar albino rats, it greatly decreased the activities of catalase (CAT) and superoxide dismutase (SOD), but it increased the activities of iNOS and the levels of MDA and IL-1β. Cyclophosphamide increased ALT (52.3 U/L), AST (48.7 U/L), ALP (46.5 U/L), total bilirubin (1.4 mg/dL), conjugated bilirubin (0.8 mg/dL), creatinine (0.8 mg/dL), urea (45.6 mg/dL), and BUN (15.7 mg/dL), with a reduction in the albumin (4.8 g/dL) level. Cyclophosphamide administration also caused hepatocellular necrosis, inflammatory leukocyte infiltration, tubular necrosis, and proteinaceous deposits in Bowman's space in the kidney. However, combining CMSO and Omega 3 oils significantly restored the altered histology architecture, antioxidant, inflammatory markers, and liver and kidney function parameters to levels comparable
Background Terminalia catappa is a traditional medicinal plant used for several ailments among the local folks. Among the locals, the stem bark is an important constituent of polyherbal remedies for the treatment of c...
Background Terminalia catappa is a traditional medicinal plant used for several ailments among the local folks. Among the locals, the stem bark is an important constituent of polyherbal remedies for the treatment of cancer and metabolic diseases; however, there is a paucity of scientific reports on it. This study aimed to evaluate the in vitro antidiabetic, antihypertensive and antierectile dysfunction effects of Terminalia catappa stem bark extract (TSBE). Methods The HPLC phytochemical profiling, total phenol content, total flavonoid content and antioxidant capacity of the extract were delineated. The inhibitory effects of TSBE on activities of α-glucosidase, α-amylase, acetylcholinesterase (AChE), arginase, phosphodiesterase-5′ (PDE-5′), angiotensin-converting enzyme (ACE) and ecto-5 nucleotidase (Ecto-5-NTD) were estimated following standard procedures. Results HPLC of TSBE profiled ferulic acid, gallic acid, caffeic acid, catechin, rutin, p -coumaric acid, chlorogenic acid, kaempferol, apiginin and quercetin. TSBE had IC 50 values of 49.19 µg/mL and 55.42 µg/mL in terms of scavenging DPPH and NO radicals, respectively. The TSBE inhibited α-glucosidase and α-amylase activities with IC 50 values of 71.53 µg/mL and 105.11 µg/mL, respectively. The inhibitory effects of TSBE on AChE, arginase, PDE-5′, and ACE revealed IC 50 values higher than that of standards. TSBE demonstrated a stimulatory effect on ecto-5-NTD activity. Conclusion TSBE revealed promising antioxidant, antidiabetic, antihypertensive and antierectile dysfunction (anti-ED) properties which may be associated with the bioactive compounds present in the extract.
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