检索结果-内蒙古大学图书馆

Correction to: Multiplexed inhibition of immunosuppressive genes with Cas13d for combinatorial cancer immunotherapy (Nature Biotechnology, (2025), 10.1038/s41587-024-02535-2)

引用

Nature Biotechnology 2025年

作者： Zhang, Feifei Chow, Ryan D. He, Emily Dong, Chuanpeng Xin, Shan Mirza, Daniyal Feng, Yanzhi Tian, Xiaolong Verma, Nipun Majety, Medha Zhang, Yueqi Wang, Guangchuan Chen, Sidi Department of Genetics Yale University School of Medicine New HavenCT United States System Biology Institute Yale University West HavenCT United States Center for Cancer Systems Biology Yale University West HavenCT United States M.D.–Ph.D. Program Yale University West HavenCT United States Molecular Cell Biology Genetics and Development Program Yale University New HavenCT United States Yale College New HavenCT United States Immunobiology Program Yale University New HavenCT United States Department of Therapeutic Radiology Yale University School of Medicine New HavenCT United States Department of Neurosurgery Yale University School of Medicine New HavenCT United States Yale Comprehensive Cancer Center Yale University School of Medicine New HavenCT United States Yale Stem Cell Center Yale University School of Medicine New HavenCT United States Yale Center for Biomedical Data Science Yale University School of Medicine New HavenCT United States Key Laboratory of Multi-Cell Systems Shanghai Institute of Biochemistry and Cell Biology Center for Excellence in Molecular Cell Science Chinese Academy of Sciences Shanghai China

Correction to: Nature Biotechnologyhttps://***/10.1038/s41587-024-02535-2, published online 16 January 2025. In the version of the article initially published, Sidi Chen was incorrectly associated with a present address. This has now been corrected in the HTML and PDF versions of the article. © The Author(s), under exclusive licence to Springer Nature America, Inc. 2025.

关键词：

来源：评论

学校读者我要写书评

暂无评论

The Effect of G. applanatum Crude Polysaccharide Extract on Proinflammatory Cytokines and Proapoptotic Caspases in HeLa Cell Line: An In Vitro Study

Advances in Pharmacological and Pharmaceutical Sciences

引用

Advances in Pharmacological and Pharmaceutical Sciences 2023年第1期2023卷

作者： Susilo, Raden Joko Kuncoroningrat Hayaza, Suhailah Fikriyah, Nur'aini Musthoza, Fina Syifa'una Islamatasya, Ufairanisa Rohmatika, Aulia Umi Winarni, Dwi Wahyuningsih, Sri Puji Astuti Doong, Ruey-An Karsari, Deya Yanti, Aristika Dinar Fahmi, Mochammad Zakki Darmanto, Win Magister Program in Biology Faculty of Science and Technology Universitas Airlangga Surabaya 60115 Indonesia Department of Nanotechnology Engineering Faculty of Advance Technology and Multidiscipline Universitas Airlangga Surabaya 60115 Indonesia Department of Biology Faculty of Science and Technology Universitas Airlangga Surabaya 60115 Indonesia Institute of Analytical and Environmental Sciences National Tsing Hua University Sec. 2 Kuang Fu Road Hsinchu 30013 Taiwan Stem Cell Research and Development Center Universitas Airlangga Surabaya 60115 Indonesia Department of Chemistry Faculty of Science and Technology Universitas Airlangga Surabaya 60115 Indonesia Institute of Science Technology and Health Jombang 61419 Indonesia

Polysaccharide extracts exhibit promise as potential anticancer agents. Among the fungi rich in polysaccharide content, G. applanatum stands out;however, its anticancer activity necessitates further investigation. This study aims to explore the impact of G. applanatum crude polysaccharide (GACP) extract by assessing its effects on cell viability, levels of proinflammatory cytokines such as TNF-α, IFN-γ, IL-2, and IL-12, and levels of proapoptotic markers including caspase-3 and caspase-9, as well as the percentages of necrosis and apoptosis in the HeLa cell line. Employing the HeLa cell line as a research model, four groups were studied: KN (media and DMSO), K+ (doxorubicin 10 μg/mL), P1 (G. applanatum extract 200 μg/mL), and P2 (G. applanatum extract 400 μg/mL). The G. applanatum extract was obtained via boiling distilled water. Anticancer activity was evaluated through the MTT test (3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide) conducted over three treatment durations (24, 48, and 72 hours). Cytokine levels and caspase-3 and caspase-9 levels were assessed using the ELISA test. Cell apoptosis was determined using the Annexin V-PI biomarker and analyzed through flow cytometry. The MTT test exhibited optimal results at the 48-hour treatment mark. Cytokine level analysis revealed significant reductions in TNF-α, IFN-γ, IL-2, and IL-12 levels (p < 0.005). Concurrently, caspase-3 and caspase-9 levels exhibited substantial increases (p < 0.005). Flow cytometry highlighted the highest percentage of apoptosis in HeLa cells. In conclusion, G. applanatum's polysaccharide extract demonstrates potential as an anticancer and therapeutic agent for cancer treatment. © 2023 Qurrotu A'yun et al.

关键词：

来源：评论

学校读者我要写书评

暂无评论

An integrated transcriptomic cell atlas of human neural organoids (vol 635, pg 690, 2024)

引用

NATURE 2025年第8045期637卷 E21-E21页

作者： He, Zhisong Dony, Leander Fleck, Jonas Simon Szalata, Artur Li, Katelyn X. Sliskovic, Irena Lin, Hsiu-Chuan Santel, Malgorzata Atamian, Alexander Quadrato, Giorgia Sun, Jieran Pasca, Sergiu P. Camp, J. Gray Theis, Fabian J. Treutlein, Barbara Department of Biosystems Science and Engineering ETH Zürich Basel Switzerland Institute of Computational Biology Computational Health Center Helmholtz Munich Neuherberg Germany Department Genes and Environment Max Planck Institute of Psychiatry Munich Germany International Max Planck Research School for Translational Psychiatry (IMPRS-TP) Munich Germany TUM School of Life Sciences Weihenstephan Technical University of Munich Freising Germany School of Computation Information and Technology Technical University of Munich Munich Germany Department of Physiology and Pharmacology Karolinska Institutet Stockholm Sweden Institute of Human Biology (IHB) Roche Pharma Research and Early Development Roche Innovation Center Basel Basel Switzerland Biozentrum University of Basel Basel Switzerland Department of Stem Cell Biology and Regenerative Medicine Keck School of Medicine University of Southern California Los Angeles CA USA Eli and Edythe Broad CIRM Center for Regenerative Medicine and Stem Cell Research at USC Keck School of Medicine University of Southern California Los Angeles CA USA Department of Psychiatry and Behavioral Sciences Stanford University Stanford CA USA Stanford Brain Organogenesis Program Wu Tsai Neurosciences Institute and Bio-X Stanford CA USA Neural Stem Cell Institute Albany NY USA UK Dementia Research Institute at the University of Edinburgh Edinburgh Bioquarter Edinburgh UK Centre for Discovery Brain Sciences School of Biomedical Sciences College of Medicine and Veterinary Medicine The University of Edinburgh Edinburgh UK Human Technopole Milan Italy Department of Oncology and Hemato-Oncology University of Milan Milan Italy

来源：评论

学校读者我要写书评

暂无评论

Mechanisms and therapeutic actions of edible fruits in inflammatory bowel disease: A review of pre-clinical studies

Food Chemistry Advances

引用

Food Chemistry Advances 2023年 3卷

作者： Arunachalam, Karuppusamy Sreeja, Puthanpura Sasidharan Yang, Xuefei Center for Studies in Stem Cells Cell Therapy and Toxicological Genetics (CeTroGen) Faculty of Medicine (FAMED) Federal University of Mato Grosso do Sul (UFMS) Mato Grosso do Sul Campo Grande Brazil Postgraduate Program in Health and Development of the Midwest Region Faculty of Medicine (FAMED) Federal University of Mato Grosso do Sul (UFMS) Mato Grosso do Sul Campo Grande Brazil Department of Botany NSS College Palakkad India Key Laboratory of Economic Plants and Biotechnology and the Yunnan Key Laboratory for Wild Plant Resources Kunming Institute of Botany Chinese Academy of Sciences Kunming China Southeast Asia Biodiversity Research Institute Chinese Academy of Sciences Nay Pyi Taw Myanmar

The review highlights the significance of traditional knowledge in using edible fruits to address health issues, specifically focusing on inflammatory bowel disease (IBD). Edible fruits are rich in beneficial components like dietary fiber, vitamins, minerals, polyphenols, bioactive peptides, and antioxidants which contribute to overall health and show potential in managing gastrointestinal disorders. The objective of the review is to provide strong evidence supporting the effectiveness of edible fruits in preventing and treating IBD. The study conducted an extensive review of research published from 2001 to 2022, gathering data from reputable sources and evaluating the utilization of edible fruits in IBD treatment. This review identified 36 species of edible fruits across 10 families historically used in traditional medicine. Various bioactive molecules like tannins, flavonoids, saponins, terpenoids, alkaloids, and ascorbic acid have been isolated from these fruits. Recent studies have shown that compounds from edible fruits can counteract intestinal inflammation in animal models by influencing key signaling pathways and exhibiting immunomodulatory, anti-inflammatory, and antioxidant properties. The review suggests that extracts from edible fruits could offer potential alternative or supplementary treatments for individuals with IBD. However, the review also acknowledges limitations in some prior studies, including inadequate dosages, insufficient standards, and an incomplete understanding of mechanisms. Further research is needed to fully elucidate the mechanisms underlying the effects of these extracts and compounds. © 2023 The Author(s)

关键词： Edible fruits Inflammasome-signaling pathway Inflammatory bowel disease Polyphenols Pre-clinical studies

来源：评论

学校读者我要写书评

暂无评论

Synergistic suppression of cholangiocarcinoma cells via DNA damage response and cell cycle arrest by dual targeting PARP and ATM in DNA damage repair pathway

引用

Biomedicine & Pharmacotherapy 2025年 189卷 118273页

作者： Rattanaporn Jaidee Piman Pocasap Apinya Jusakul Laddawan Senggunprai Auemduan Prawan Jing Han Hong Hong Lee Heng Veerapol Kukongviriyapan Bin Tean Teh Sarinya Kongpetch Department of Pharmacology Faculty of Medicine Khon Kaen University Khon Kaen Thailand Cholangiocarcinoma Research Institute Khon Kaen University Khon Kaen Thailand Centre for Research and Development of Medical Diagnostic Laboratories Faculty of Associated Medical Sciences Khon Kaen University Khon Kaen Thailand Cancer and Stem Cell Biology Program Duke-NUS Medical School Singapore Laboratory of Cancer Epigenome Division of Medical Science National Cancer Centre of Singapore Singapore

This study investigated alterations in DNA damage repair (DDR) genes in cholangiocarcinoma (CCA) influencing cellular vulnerability to DDR inhibitors, notably poly (ADP-ribose) polymerase inhibitors (PARPi) and ataxia-telangiectasia mutated inhibitors (ATMi). Genomic DDR alterations in CCA tumors were identified via cBioPortal. The cytotoxic effect of the inhibitors was determined in 8 CCA cell lines. Cell cycle arrest and apoptosis were determined using flow cytometry. The levels of γ-H2A histone family member X (γ-H2AX) were evaluated using an immunofluorescence assay. Proteins associated with the cell cycle and DDR were evaluated using Western blotting. Among these CCA cells, BRCA- mutated KKU-023 cells exhibited the highest PARPi sensitivity. Co-treatment with ATMi induced a synthetic homologous recombination deficiency (HRD) state in BRCA- proficient CCA cells, thereby sensitizing them to PARPi. This drug combination suppressed CCA cell growth via G2/M phase arrest, downregulated cyclin A and B1 expression, and enhanced apoptosis. Mechanistically, this synergistic cytotoxicity was attributed to increased DNA double-strand breaks, evidenced by elevated γ-H2AX and reduced RAD51 expression, signifying compromised DNA repair. These preclinical findings support the therapeutic potential of DDR inhibitors, individually or in combination, for CCA subsets characterized by DDR gene alterations.

关键词： Olaparib Niraparib ATM inhibitor Synthetic lethality DNA damage repair Cholangiocarcinoma

来源：评论

学校读者我要写书评

暂无评论

Shedding Light Onto the Structural Secrets Inside Pluripotent stem Cells of the Preimplantation Mammalian Embryo in Real-Time

引用

Fertility & Reproduction 2022年第3N04期4卷 125-125页

作者： Asma ABERKANE Azelle HAWDON Gemma STATHATOS Jessica GREANEY Yi Louise LI Xiaodong LIU Rob PARTON José POLO Jennifer ZENKER Australian Regenerative Medicine Institute Monash University Clayton Victoria Australia Department of Anatomy and Developmental Biology Monash University Clayton Victoria Australia Development and Stem Cells Program Monash Biomedicine Discovery Institute Clayton Victoria Australia Institute for Molecular Bioscience University of Queensland Australia

The organisation of a cell’s interior, the cytoskeleton and organelles, is fundamental for every cell’s functionality. However, unlike most differentiated cells, our knowledge about the contribution of the sub-cellular architecture to embryonic development and pluripotency remains scarce. Using advanced live imaging, we discovered polarised non-centrosomal microtubules as central player for the pluripotent state of the in vivo early mammalian embryo and induced pluripotent stem cells (iPSCs). Each cell contains an apical pole highly enriched with CAMSAP3-nucleated microtubules, growing in a longitudinal direction towards the base of PSCs. These non-centrosomal microtubules initiate an asymmetry of metabolites and organelles, including mitochondria. We further establish their rearrangement upon differentiation in a germ layer-specific manner. Dissecting how intrinsic cellular regulation contributes to pluripotency will lead a revolutionary era of regenerative and reproductive medicine.

关键词：

来源：评论

学校读者我要写书评

暂无评论

Surveying brain tumor heterogeneity by single-cell RNA-sequencing of multi-sector biopsies

引用

National Science Review 2020年第8期7卷 1306-1318页

作者： Kai Yu Yuqiong Hu Fan Wu Qiufang Guo Zenghui Qian Waner Hu Jing Chen Kuanyu Wang Xiaoying Fan Xinglong Wu John EJ Rasko Xiaolong Fan Antonio Iavarone Tao Jiang Fuchou Tang Xiao-Dong Su Biomedical Pioneering Innovation Center (BIOPIC) School of Life Sciences Peking University Beijing Advanced Innovation Center for Genomics School of Life Sciences Peking University Biomedical Institute for Pioneering Investigation via Convergence and Center for Reproductive Medicine Ministry of Education Key Laboratory of Cell Proliferation and Differentiation Department of Molecular Neuropathology Beijing Neurosurgical Institute Capital Medical University Department of Neurosurgery Beijing Tiantan Hospital Capital Medical University Chinese Glioma Genome Atlas Network (CGGA) and Asian Glioma Genome Atlas Network (AGGA) Peking-Tsinghua Center for Life Sciences Peking University Gene and Stem Cell Therapy Program Centenary Institute University of Sydney Department of Cell and Molecular Therapies Royal Prince Alfred Hospital Beijing Key Laboratory of Gene Resource and Molecular Development Laboratory of Neuroscience and Brain Development Beijing Normal University Institute for Cancer Genetics Herbert Irving Comprehensive Cancer Center Columbia University Medical Center Department of Pathology & Cell Biology Columbia University Medical Center Department of Neurology Columbia University Medical Center Center of Brain Tumor Beijing Institute for Brain Disorders

Brain tumors are among the most challenging human tumors for which the mechanisms driving progression and heterogeneity remain poorly understood. We combined single-cell RNA-seq with multi-sector biopsies to sample and analyze single-cell expression profiles of gliomas from 13 Chinese patients. After classifying individual cells, we generated a spatial and temporal landscape of glioma that revealed the patterns of invasion between the different sub-regions of gliomas. We also used single-cell inferred copy number variations and pseudotime trajectories to inform on the crucial branches that dominate tumor *** dynamic cell components of the multi-region biopsy analysis allowed us to spatially deconvolute with unprecedented accuracy the transcriptomic features of the core and those of the periphery of glioma at single-cell level. Through this rich and geographically detailed dataset, we were also able to characterize and construct the chemokine and chemokine receptor interactions that exist among different tumor and non-tumor cells. This study provides the first spatial-level analysis of the cellular states that characterize human gliomas. It also presents an initial molecular map of the cross-talks between glioma cells and the surrounding microenvironment with single-cell resolution.

关键词： glioma heterogeneity multi-sector biopsy single-cell RNA-seq

来源：评论

学校读者我要写书评

暂无评论

Washington’s ClimeTime: Collaboration and Capacity-Building to Foster Climate Learning Across a State Network

引用

Connected Science Learning 2024年第4期6卷

作者： Ellen Ebert Johanna Brown Elizabeth Schmitz Deb L. Morrison Philip Bell Cheryl Lydon Luke Matlack a Director of Secondary Education Office of the Superintendent of Public Instruction Olympia WA b Associate Director of Secondary Science Office of the Superintendent of Public Instruction Olympia WA c Program Supervisor Environment and Sustainability Education Office of the Superintendent of Public Instruction Olympia WA d Learning Scientist Institute of Science and Math Education College of Education University of Washington Seattle WA e Professor Learning Sciences & Human Development College of Education University of Washington Seattle WA f Program Manager STEM Puget Sound Educational Service District Renton WA g Regional Science Coordinator Educational Service District 105 Yakima WA

Washington State’s ClimeTime Network is a groundbreaking effort that has been implementing systemic capacity-building around climate science learning, as well as broader scientific literacy, for the past 6 years. This article provides an overview of the ClimeTime Network formation, framing, and implementation strengths and challenges. Additionally, the core commitments that help guide ongoing implementation efforts are detailed. The organization of the network’s ongoing operations are described and further guidance is provided to help others who are engaging in emerging systemic efforts to support climate change education and the required *** AbstractDisplay full size

关键词： Climate science climate change education climate learning professional development networks science learning community-connected learning equity in education

来源：评论

学校读者我要写书评

暂无评论

Micro-Chimerism Detected By Highly Sensitive AlloHeme TM Solution Is Associated with Treatment Response and Relapse after Allogeneic stem Cell Transplantation

引用

Transplantation and Cellular Therapy 2024年第2期30卷 S403-S404页

作者： Kothari, Ashish S, MD, MS Dwivedi, Nishant, MD, PhD Chattopadhyay, Shrikanta, MD Wang, Yun, PhD Zhou, Mingming Vaskova, Evgeniya, PhD Viard, Thierry, PhD Wong, Lily, MS Gulbahce, Natali, PhD Qu, Kunbin, PhD Grskovic, Marica, PhD Kongtim, Piyanuch, MD, PhD Ciurea, Stefan O., MD Clinical Development CareDx Brisbane CA SVP Medical TScan Therapeutics Waltham MA TScan Therapeutics Waltham MA Biostatistics and Data Science CareDx Inc Brisbane CA R&D CareDx Brisbane CA CareDx Inc Brisbane CA Bioinformatics CareDx Brisbane CA Data Science and Biostatistics CareDx Inc Brisbane CA Cell Transplant Therapy CareDx Inc Brisbane CA Division of Hematology/Oncology Department of Medicine Hematopoietic Stem Cell Transplantation and Cellular Therapy Program University of California Irvine Orange CA

Post-transplant chimerism status is associated with graft survival and potentially disease relapse after allogeneic stem cell transplantation (allo-HCT). However, detection of low-level micro-chimerism (MC) following allo-HCT using the current standard with short tandem repeats (STR) PCR method has been challenging due to STR's poor limit of detection (LOD) of 1-5%. A more sensitive method of chimerism detection could help inform early treatment decisions. We present preliminary results from two multi-center trials using a next-generation sequencing (NGS)-based MC detection method, AlloHeme TM (AH), with a higher sensitivity (LOD 0.035-0.043%) (Table 1), as a translational biomarker of treatment response and early relapse, including minimal residual disease (MRD), after allo-HCT for acute leukemias and MDS. Trial 1: NCT04635384 (ACROBAT, CareDx Inc.) is a prospective multi-center observational cohort study. AlloHeme is the study's primary biomarker. ACROBAT has fully enrolled 289 subjects, with a median follow-up of 150 days. Trial 2: NCT05473910 (TSCAN-001, TScan Inc.) is a multi-arm non-randomized controlled Phase 1 study evaluating the safety and efficacy of TScan's TSC100 and TSC101 - allogeneic T-cell receptor-engineered T cells that target haematopoietically restricted antigens HA-1 and HA-2 respectively, both presented on HLA-A*02:01, in mismatched reduced-intensity conditioning (RIC)-haplo donor HCT recipients. AlloHeme is used as a translational biomarker. Complete chimerism (CC) was defined as >99.9% recipient chimerism. Early review of the ongoing ACROBAT study data with CD33 CC (N=73; median follow-up 270 days, range 72-608 days) revealed three relapses (4%), which compares favorably with 18-20% 6-month relapse rates in the CIBMTR data, suggestive of prognostic potential. This data is corroborated by the preliminary findings of Trial 2. Increased MC (iMC) was defined as ≥1% increase in recipient chimerism between two consecutive AH results. Patients with

关键词：

来源：评论

学校读者我要写书评

暂无评论

Author Correction: Niche-derived Semaphorin 4A safeguards functional identity of myeloid-biased hematopoietic stem cells

Nature aging

引用

Nature aging 2025年 2025 Feb 20页

作者： Dorsa Toghani Sanika Gupte Sharon Zeng Elmir Mahammadov Edie I Crosse Negar Seyedhassantehrani Christian Burns David Gravano Stefan Radtke Hans-Peter Kiem Sonia Rodriguez Nadia Carlesso Amogh Pradeep Alexis Georgiades Fabienne Lucas Nicola K Wilson Sarah J Kinston Berthold Göttgens Le Zong Isabel Beerman Bongsoo Park Derek H Janssens Daniel Jones Ali Toghani Claus Nerlov Eric M Pietras Marion Mesnieres Christa Maes Atsushi Kumanogoh Thomas Worzfeld Jin-Gyu Cheong Steven Z Josefowicz Peter Kharchenko David T Scadden Antonio Scialdone Joel A Spencer Lev Silberstein Translational Science and Therapeutics Division Fred Hutchinson Cancer Research Center Seattle WA USA. Institute of Epigenetics and Stem Cells (IES) Helmholtz Zentrum Muenchen Munich Germany. Department of Bioengineering University of California Merced Merced CA USA. Department of Stem Cell Biology & Regenerative Medicine Beckman Research Institute of City of Hope Duarte CA USA. Department of Haematology Jeffrey Cheah Biomedical Centre Wellcome - MRC Cambridge Stem Cell Institute University of Cambridge Cambridge UK. Epigenetics and Stem Cell Aging Unit National Institute of Aging Baltimore MD USA. Department of Epigenetics Van Del Institute Grand Rapids MI USA. Radcliffe Department of Medicine University of Oxford Oxford UK. Department of Medicine-Hematology University of Colorado School of Medicine Aurora CO USA. Department of Development and Regeneration KU Leuven Leuven Belgium. Department of Respiratory Medicine Allergy and Rheumatic Diseases University of Osaka Osaka Japan. Faculty of Medicine Institute of Pharmacology University of Marburg Marburg Germany. Department of Pathology and Laboratory Medicine Weill Cornell Medicine New York NY USA. Immunology and Microbial Pathogenesis Program Weill Cornell Medicine New York NY USA. Department of Stem Cell and Regenerative Biology Harvard University Boston MA USA. Translational Science and Therapeutics Division Fred Hutchinson Cancer Research Center Seattle WA USA. lsilbers@***.

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：