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High-resolution two-photon transcranial imaging of brain using direct wavefront sensing

Photonics Research 2021年第6期9卷 1144-1156页

作者： CONGPING CHEN ZHONGYA QIN SICONG HE SHAOJUN LIU SHUN-FAT LAU WANJIE WU DAN ZHU NANCY Y.IP JIANAN Y.QU Department of Electronic and Computer Engineering The Hong Kong University of Science and TechnologyClear Water BayKowloonHong KongChina State Key Laboratory of Molecular Neuroscience The Hong Kong University of Science and TechnologyClear Water BayKowloonHong KongChina Center of Systems Biology and Human Health The Hong Kong University of Science and TechnologyClear Water BayKowloonHong KongChina Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for OptoelectronicsHuazhong University of Science and TechnologyWuhanChina MoE Key Laboratory for Biomedical Photonics Collaborative Innovation Center for Biomedical EngineeringSchool of Engineering SciencesHuazhong University of Science and TechnologyWuhanChina Division of Life Science The Hong Kong University of Science and TechnologyClear Water BayKowloonHong KongChina Molecular Neuroscience Center The Hong Kong University of Science and TechnologyClear Water BayKowloonHong KongChina

Imaging of the brain in its native state at high spatial resolution poses major challenges to visualization ***-photon microscopy integrated with the thinned-skull or optical clearing skull technique provides a minimally invasive tool for in vivo imaging of the cortex of mice without activating immune response and inducing brain ***,the imaging contrast and spatial resolution are severely compromised by the optical heterogeneity of the skull,limiting the imaging depth to the superficial *** this work,an optimized configuration of an adaptive optics two-photon microscope system and an improved wavefront sensing algorithm are proposed for accurate correction for the aberrations induced by the skull window and brain *** this system,we achieved subcellular resolution transcranial imaging of layer 5 pyramidal neurons up to 700μm below pia in living *** addition,we investigated microglia–plaque interaction in living brain of Alzheimer’s disease and demonstrated high-precision laser dendrotomy and single-spine ablation.

关键词： resolution photon system

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Co-localization of two-color rAAV2-retro confirms the dispersion characteristics of efferent projections of mitral cells in mouse accessory olfactory bulb

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Zoological Research 2020年第2期41卷 148-156页

作者： Ning Zheng Zhi-Zhong Wang Song-Wei Wang Fang-Jia Yang Xu-Tao Zhu Chen Lu Anne Manyande Xiao-Ping Rao Fu-Qiang Xu Center of Brain Science State Key Laboratory of Magnetic Resonance and Atomic and Molecular PhysicsNational Center for Magnetic Resonance in WuhanKey Laboratory of Magnetic Resonance in Biological SystemsWuhan Institute of Physics and MathematicsInnovation Academy for Precision Measurement Science and TechnologyChinese Academy of SciencesWuhanHubei 430071China University of the Chinese Academy of Sciences Beijing 100049China Department of Automation School of Electrical EngineeringZhengzhou UniversityZhengzhouHenan 450001China School of Life Science Wuhan UniversityWuhanHubei 430072China School of Human and Social Sciences University of West LondonMiddlesex TW89GAUK Divisions of Biomedical Photonics Wuhan National Laboratory for OptoelectronicsWuhanHubei 430074China

The accessory olfactory bulb(AOB), located at the posterior dorsal aspect of the main olfactory bulb(MOB), is the first brain relay of the accessory olfactory system(AOS), which can parallelly detect and process volatile and nonvolatile social chemosignals and mediate different sexual and social behaviors with the main olfactory system(MOS). However, due to its anatomical location and absence of specific markers, there is a lack of research on the internal and external neural circuits of the AOB. This issue was addressed by singlecolor labeling and fluorescent double labeling using retrograde rAAVs injected into the bed nucleus of the stria terminalis(BST), anterior cortical amygdalar area(ACo), medial amygdaloid nucleus(MeA), and posteromedial cortical amygdaloid area(PMCo) in mice. We demonstrated the effectiveness of this AOB projection neuron labeling method and showed that the mitral cells of the AOB exhibited efferent projection dispersion characteristics similar to those of the MOB. Moreover, there were significant differences in the number of neurons projected to different brain regions, which indicated that each mitral cell in the AOB could project to a different number of neurons in different cortices. These results provide a circuitry basis to help understand the mechanism by which pheromone information is encoded and decoded in the AOS.

关键词： Accessory olfactory bulb Efferent projections Retrograde rAAVs Projection neuron labeling Dispersion characteristics Circuitry basis

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Synchronous Motion Generation of Multiple Continuum Robots Based on a Jacobian-Estimation Strategy

Synchronous Motion Generation of Multiple Continuum Robots B...

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IEEE International Conference on robotics and Biomimetics

作者： Ning Tan Ruikun Hu Yuyang Wu Xinyu Zhang Fenglei Ni Zhenglong Sun School of Computer Science and Engineering Sun Yat-sen University Guangzhou PR China State Key Laboratory of Robotics and Systems (HIT) Harbin PR China Engineering Research Center of Software/Hardware Co-Design Technology and Application Ministry of Education (East China Normal University) Shanghai PR China School of Science and Engineering The Chinese University of Hong Kong Shenzhen China Shenzhen Institute of Artificial Intelligence and Robotics for Society Shenzhen China

ISBN: (纸本)9781665405362

In this paper, a novel model-free control scheme is proposed for synchronous motion control of multiple continuum robots. The control problem is formulated as two convex optimization routines in an adaptive and model-free closed-loop framework. The proposed approach is heading to address cooperative task execution for multiple continuum robots. The model-free feedback control guarantees the high portability for a wide range of continuum robots. Simulation studies are conducted on two kinds of tasks, which are cooperative task execution with two robots and simultaneously tracking control with three robots. Besides, the superiority of proposed approach is validated through comparisons with other methods. Simulation results prove the high accuracy and efficiency of this model-free feedback control scheme.

关键词： Jacobian matrices Adaptation models Tracking Simulation Conferences Convex functions Feedback control

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Spatially Controlled Co-Delivery of Diagnostic and Therapeutic Agents Using DNA Nanoframeworks for Pancreatic Cancer Precision Therapy

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Angewandte Chemie 2025年

作者： Nachuan Song Ruoyu Tao Hongjin Li Rui Zhang Yan Huang Le Zhang Ying Liu Dayong Yang Chi Yao State Key Laboratory of Synthetic Biology Frontiers Science Center for Synthetic Biology Key Laboratory of Systems Bioengineering (MOE) School of Chemical Engineering and Technology Tianjin 300350 P.R. China Department of Chemistry State Key Laboratory of Molecular Engineering of Polymers Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials College of Chemistry and Materials Fudan University Shanghai 200438 P.R. China CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety & CAS Center for Excellence in Nanoscience National Center for Nanoscience and Technology of China Beijing 100190 P.R. China

Theranostic platforms that integrate diagnostic and therapeutic functionalities offer promising strategies for precision medicine, particularly in the treatment of major diseases. However, the development of platforms capable of achieving spatially controlled detection and therapy at the lesion site remains a significant challenge. Herein, we present a dual-stimuli-responsive DNA nanoframework that achieve spatially controlled codelivery of molecular beacon (MB) and Cas9 ribonucleoprotein (RNP), enabling simultaneous specific optical detection and efficient gene therapy for pancreatic cancer. The DNA nanoframeworks are synthesized via precipitation polymerization, utilizing acrylamide-modified DNA to initiate a hybridization chain reaction that facilitates the effective loading of MB-extended and sgRNA-conjugated DNA hairpins. The Cas9 protein is efficiently loaded into the nanoframeworks through phase transition-induced polymer chain rearrangement, overcoming steric hindrance. Upon aptamer-mediated internalization into PANC-1 cells, the overexpressed apurinic/apyrimidinic endonuclease 1 and ribonuclease H in cancer cells induce site-specific cleave of MB and DNA–RNA hybrid duplex, respectively. This cleavage restores fluorescence for specific optical detection, whereas the released Cas9 RNP performs gene editing for efficient therapy. Low fluorescence background and favorable biocompatibility are observed in normal cells. In a pancreatic cancer mouse model, the platform demonstrates significant detection-guided antitumor efficacy, highlighting its potential for precision medicine in cancer therapy.

关键词： DNA nanotechnology Gene editing Gene therapy Pancreatic cancer

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Longitudinal landscape of immune reconstitution after acute SARS-CoV-2 infection at single-cell resolution

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Science Bulletin 2025年第3期70卷 323-327页

作者： Lili Ren Yiwei Liu Yeming Wang Chao Wu Li Guo Lan Chen Xinming Wang Yan Xiao Lixue Huang Jingchuan Zhong Jiacheng Yao Lu Liu Hui Li Ying Wang Yongchao Ma Yichunzi Zhang Lin Di Tao Dong Julian Knight Jianbin Wang Yanyi Huang Bin Cao Xianwen Ren Jianwei Wang National Health Commission Key Laboratory of Systems Biology of Pathogens and Christophe Mérieux Laboratory National Institute of Pathogen BiologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 102629China Key Laboratory of Respiratory Disease Pathogenomics Chinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China State Key Laboratory of Respiratory Health and Multimorbidity Chinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100029China Department of Pulmonary and Critical Care Medicine Institute of Respiratory Medicine of Chinese Academy of Medical ScienceNational Center for Respiratory MedicineCenter of Respiratory MedicineNational Clinical Research Center for Respiratory DiseasesChina-Japan Friendship HospitalBeijing 100029China College of Chemistry and Molecular Engineering Peking UniversityBeijing 100871China Biomedical Pioneering Innovation Center(BIOPIC) Beijing Advanced Innovation Center for Genomics(ICG)College of Chemistryand Peking-Tsinghua Center for Life SciencesPeking UniversityBeijing 100871China MRC Human Immunology Unit MRC Weatherall Institute of Molecular MedicineRadcliffe Department of MedicineThe University of OxfordOxford OX39DSUK Chinese Academy of Medical Science(CAMS)Oxford Institute(COI) University of OxfordOxford OX39DSUK Wellcome Centre for Human Genetics University of OxfordOxford OX39DSUK School of Life Sciences Tsinghua-Peking Center for Life SciencesTsinghua UniversityBeijing 100084China Beijing Advanced Innovation Center for Structural Biology(ICSB) Tsinghua UniversityBeijing 100084China Chinese Institute for Brain Research(CIBR) Beijing 102206China Institute for Cell Analysis Shenzhen Bay LaboratoryShenzhen 518107China Tsinghua University-Peking University Joint Center for Life Sciences Beijing 100084China Biomedical Pioneering Innovation Center(BIOPIC) Peking UniversityBeijing 100871China Changping Laboratory Beijing 102206China

The long-term consequences of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)infection,known as the post-acute sequelae of COVID-19(PASC),have been a growing concern.A significant proportion of COVID-19 patients experience persistent health issues even six to twelve months after recovering from acute infection[1],[2],[3],[4].Therefore,understanding the immune reconstitution process and identifying the molecular and cellular mechanisms underlying PASC is crucial.

关键词： landscape consequences acute

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A parallel tonotopically arranged thalamocortical circuit for sound processing

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Neuron 2025年第12期113卷 1998-2013.e6页

作者： Zhao, Zhikai Tang, Xiaojing Chen, Yiheng Tao, Jie Polat, Mahiber Yang, Zhiqi Yang, Linhan Wang, Meng Liang, Shanshan Zhang, Kuan Zhang, Yun Zhang, Chunqing Wang, Lina Wang, Yanjiang Konnerth, Arthur Jia, Hongbo Xiong, Wei Liao, Xiang Li, Sunny C. Chen, Xiaowei Center for Neurointelligence School of Medicine Chongqing University Chongqing 400044 China Brain Research Center and State Key Laboratory of Trauma and Chemical Poisoning Third Military Medical University Chongqing 400038 China LFC Laboratory and Chongqing Institute for Brain and Intelligence Guangyang Bay Laboratory Chongqing 400064 China Advanced Institute for Brain and Intelligence School of Medicine Guangxi University Nanning 530004 China Institute of Brain and Intelligence Third Military Medical University Chongqing 400038 China LFC Laboratory and State Key Laboratory of Science and Technology on Aerospace Intelligence Control Beijing Aerospace Automatic Control Institute Beijing 100854 China Institute of Neuroscience and Munich Cluster for Systems Neurology Technical University Munich Munich 80802 Germany Leibniz Institute for Neurobiology Magdeburg 39118 Germany Brain Research Instrument Innovation Center Suzhou Institute of Biomedical Engineering and Technology Chinese Academy of Sciences Suzhou 215163 China Chinese Institute for Brain Research Beijing 102206 China NewLight Neuroscience Unit Chongqing 400064 China

The perception of the sensory world in mammals requires information flow from the thalamus to the cortex. Although the first-order sensory thalamus and its surrounding nuclei are considered the major hub for feedforward thalamocortical transmission, it remains unknown whether any other thalamic input could also contribute to this transmission. We found a thalamic region, the basal region of the ventromedial nucleus of the thalamus (bVM), that sends dense, tonotopically arranged projections to auditory cortex (AuC) fields. Silencing these AuC-projecting neurons severely impaired the mouse's ability to discriminate sound frequencies. These projections exhibited strong frequency-tuning preferences that matched the cortical tonotopic map. Moreover, bVM inputs were excitatory and primarily terminated on neuron-derived neurotrophic factor-positive interneurons in cortical layer 1. Silencing these inputs significantly reduced sound-evoked responses of AuC neurons. Our results reveal a non-canonical, tonotopically arranged thalamic input to cortical layer 1 that contributes to sound processing, in parallel to the classic auditory thalamocortical pathway. © 2025 Elsevier Inc.

关键词： auditory cortex cortical layer 1 sound processing thalamocortical pathway thalamus

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Roles of oral and gut microbiota in acute myocardial infarction

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Journal of Advanced Research 2024年

作者： Li, Yu-Lin Chen, Bo-Yan Feng, Ze-Hao Zhou, Lu-Jun Liu, Ting Lin, Wen-Zhen Zhu, Hong Xu, Shuo Bai, Xue-Bing Meng, Xiao-Qian Zhang, Jun Liu, Yan Pu, Jun Jiang, Meng Duan, Sheng-Zhong Laboratory of Oral Microbiota and Systemic Diseases Shanghai Ninth People's Hospital College of Stomatology Shanghai Jiao Tong University School of Medicine National Center for Stomatology National Clinical Research Center for Oral Diseases Shanghai Key Laboratory of Stomatology Shanghai Research Institute of Stomatology Shanghai China Department of General Dentistry Shanghai Ninth People's Hospital College of Stomatology Shanghai Jiao Tong University School of Medicine National Center for Stomatology National Clinical Research Center for Oral Diseases Shanghai Key Laboratory of Stomatology Shanghai Research Institute of Stomatology Shanghai China Division of Cardiology State Key Laboratory of Systems Medicine for Cancer Renji Hospital School of Medicine Shanghai Jiao Tong University 160 Pujian Road Shanghai 200127 China Department of Cardiology Tongren Hospital Shanghai Jiao Tong University School of Medicine Shanghai China Department of Periodontology Shanghai Ninth People's Hospital College of Stomatology Shanghai Jiao Tong University School of Medicine National Center for Stomatology National Clinical Research Center for Oral Diseases Shanghai Key Laboratory of Stomatology Shanghai Research Institute of Stomatology Shanghai China Stomatology Hospital School of Stomatology Institute of Translational Medicine Zhejiang University School of Medicine Zhejiang Provincial Clinical Research Center for Oral Diseases Key Laboratory of Oral Biomedical Research of Zhejiang Province Cancer Center of Zhejiang University Engineering Research Center of Oral Biomaterials and Devices of Zhejiang Province Zhejiang Hangzhou China

Introduction: The significance of oral/gut microbiota in acute myocardial infarction (AMI) has been increasingly appreciated. However, correlations between oral/gut microbiota and AMI parameter, as well as the key microbiota that may have a crucial function in this process, remain unclear. Objectives: To investigate the composition and structure of oral and gut microbiota associated with AMI and explore the roles of specific bacterial species in the progression of AMI. Methods: We conducted a case-control study with 37 AMI patients and 36 controls. Oral and gut sample were collected and sequenced. Using correlation analysis, we combined bioinformatics data with AMI clinical parameters and obtained heatmaps of correlation coefficients. Additionally, we used antibiotics to eliminate the gut microbiota of C57BL/6J mice, followed by the transplantation of selected bacteria to verify the gut colonization of oral bacteria and their impact on AMI. Results: The component of oral and gut microbiota of AMI group showed significant alterations when compared to the control group. 17 salivary genera, 21 subgingival genera, and 8 gut genera in AMI group substantially differed from those in control group. Additionally, 19 genera from saliva, 19 genera from subgingival plaque, and 11 genera from feces substantially correlated with AMI clinical parameters. Orally administrated S.o (Streptococcus oralis subsp. dentisani), S.p (Streptococcus parasanguinis), and S.s (Streptococcus salivarius) were able to colonize in the gut and exacerbate myocardial infarction. Conclusion: There is a strong correlation between oral/gut microbiota and AMI. Streptococcus spp. is capable to transmit from oral to gut and exacerbate myocardial infarction in mice. Monitoring and control of specific oral microbiota may be an effective new strategy for improving the therapy of AMI. © 2024

关键词： Acute myocardial infarction Gut microbiota Oral microbiota Oral-gut transmission Streptococcus

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Fundamental insights into Brønsted acid-induced adduction improve small metabolite profiling in mass spectrometry

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Chemical engineering Journal 2025年 515卷

作者： Liu, Wanshan Chen, Wei Li, Yanyan Yang, Shouzhi Cao, Jing Wu, Jiao Huang, Yida Wang, Ruimin Chen, Xiaonan Huang, Lin Wang, Jiayi Qian, Kun Wan, Jingjing State Key Laboratory of Systems Medicine for Cancer School of Biomedical Engineering Institute of Medical Robotics and Shanghai Academy of Experimental Medicine Shanghai Jiao Tong University Shanghai200030 China Division of Cardiology Renji Hospital School of Medicine Shanghai Jiao Tong University Shanghai200127 China School of Chemistry and Molecular Engineering East China Normal University Shanghai200241 China Department of Clinical Laboratory Medicine Shanghai Institute of Thoracic Oncology Shanghai Chest Hospital Shanghai Jiao Tong University Shanghai200030 China

Mass spectrometry (MS) is the primary tool to detect small metabolite ions for profiling bio-systems. However, small metabolite profiling in MS still needs to be improved and calls for new rules in ionization. Herein, we studied the Brønsted acid-induced multiple alkali metal cation adductions (BAI-mAMCA) in laser desorption/ionization MS, with annotation of BAI-mAMCA in complex bio-samples and improved profiling of small metabolites. Firstly, we engineered the enhanced mAMCA (relative intensities increased from 0.1–4.6 % to 76.1–89.9 %, p 2 = 0.989–0.997, p © 2025

关键词： Metabolites

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3D Bioprinting:A Novel Avenue for Manufacturing Tissues and Organs

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engineering 2019年第4期5卷 777-794页

作者： Bin Zhang Lei Gao Liang Ma Yichen Luo Huayong Yang Zhanfeng Cui State Key Laboratory of Fluid Power&Mechatronic Systems Zhejiang UniversityHangzhou 310058China School of Mechanical Engineering Zhejiang UniversityHangzhou 310058China Institute of Biomedical Engineering Department of Engineering ScienceUniversity of OxfordOxford OX3 7DQUK

Three-dimensional(3D)bioprinting is a rapidly growing technology that has been widely used in tissue engineering,disease studies,and drug *** provides the unprecedented capacity of depositing various types of biomaterials,cells,and biomolecules in a layer-by-layer fashion,with precisely controlled spatial *** technology is expected to address the organ-shortage issue in the *** this review,we first introduce three categories of 3D bioprinting strategies:inkjet-based printing(IBP),extrusion-based printing(EBP),and light-based printing(LBP).Biomaterials and cells,which are normally referred to as“bioinks,”are then *** also systematically describe the recent advancements of 3D bioprinting in fabricating cell-laden artificial tissues and organs with solid or hollow structures,including cartilage,bone,skin,muscle,vascular network,and so *** development of organs-onchips utilizing 3D bioprinting technology for drug discovery and toxicity testing is reviewed as ***,the main challenges in current studies and an outlook of the future research of 3D bioprinting are discussed.

关键词： Three-dimensional bioprinting Solid tissues Hollow tissues Organs-on-chips Tissue engineering Drug screening

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Animal Experimental Study on Delayed Implantation in a Severely Atrophic Alveolar Ridge Reconstructed Using a 3D-Printed Bioactive Glass Scaffold: A Pilot Study

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Journal of functional biomaterials 2025年第5期16卷 176页

作者： Lei Deng Liya Ai Runxu Li Wusheng Xu Lingling Zheng Chao Wang Haitao Huang Department of Stomatology The First Affiliated Hospital of Dalian Medical University Dalian 116011 China. Key Laboratory of Biomechanics and Mechanobiology Ministry of Education Beijing Advanced Innovation Center for Biomedical Engineering School of Biological Science and Medical Engineering School of Engineering Medicine Beihang University No. 37 Xueyuan Road Beijing 100083 China. State Key Laboratory of Virtual Reality Technology and Systems Beihang University No. 37 Xueyuan Road Beijing 100083 China.

In this study, a scaffold was designed using 3-Matic software 12.0 (Materialise, Leuven, Belgium) and fabricated via Digital Light Processing (DLP) 3D printing technology, followed by a mechanical property evaluation. The scaffold was bilaterally implanted into mandibular bone defect models in four Beagle dogs to facilitate guided alveolar bone regeneration. After 12 weeks, samples were harvested from two dogs for radiographic and histopathological evaluations. In the remaining two dogs, two dental implants were placed into the scaffold sites. After an additional 12 weeks, samples were harvested for further radiographic and histopathological assessments. (1) Compression testing of the scaffold demonstrated a compressive strength of 24.77 ± 2.36 MPa. (2) Three of the implantation sites exhibited poor wound healing and exposure of the bone grafts early post-surgery (4 weeks), with an exposure rate of 37.5%. (3) Micro-CT imaging revealed a uniform distribution of newly formed bone within the scaffold, with an average bone height of 4.05 ± 0.55 mm and a bone volume fraction of 43.93 ± 4.68%. Histopathological analysis demonstrated the presence of vascularized tissue, non-calcified bone, and newly calcified bone within the scaffold. Additionally, newly formed calcified bone and vascularized tissue were observed at the interface between the implant and the scaffold. These findings suggest that DLP 3D-printed A-W bioactive glass scaffolds represent a promising approach for guided alveolar bone regeneration in dental implant applications.

关键词： 3D printing A-W bioactive glass dental implant guided alveolar bone regeneration

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