Recombinant protein production in Escherichia coli (E. coli) is crucial for biotechnological applications, but optimizing protein titer is challenging and often requires iterative experimentation and expert guidance. ...
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Current biomanufacturing processes rely heavily on human expertise, struggling to adapt to the growing complexity of bioprocessing. Decision support tools based on machine learning models play a vital role in optimizi...
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A preliminary work of our study was published at IJCAI’21 [47], which is substantially extended in the following aspects: (1) In Section 1, we analyze the necessity of introducing item attributes for detecting unreli...
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A preliminary work of our study was published at IJCAI’21 [47], which is substantially extended in the following aspects: (1) In Section 1, we analyze the necessity of introducing item attributes for detecting unreliable instances, together with the problems and challenges that attributes may bring in. (2) In Section 2, we add discussions about the limitations of existing attribute-aware recommender systems (Section 2.2) and denoising methods (Section 2.3) in the context of detecting unreliable instances. (3) In Section 4.2, we further conduct an in-depth analysis at the attribute level to demonstrate the capability of attributes for rectifying instance loss and uncertainty, as well as the disturbance caused by attributes. (4) We generalize BERD to a generic framework BERD+ in Section 5.1, equipped with novel modules, i.e., HU-GCN (Section 5.2) and EPE (Section 5.4), which properly incorporate item attributes while reducing their disturbance for rectifying instance uncer tainty (Section 5.5) and loss (Section 5.6). The generic BERD+ can be flexibly plugged into existing SRSs for performance enhanced recommendation via eliminating unreliable data. (5) In Section 6.2, we apply our BERD+ framework to seven state-of-the-art SRSs on five real-world datasets to illustrate its superiority. (6) To avoid unfair comparison caused by item attributes, we build and compare with the baseline that combines the original BERD and an advanced attribute-aware recommender system, KSR [19]. (7) For more comprehensive comparison, in Section 6.2.2, we compare BRED+ with two state-of-the-art denoising approaches;in Section 6.2.3, to examine the efficacy of HU-GCN and EPE, we compare HU-GCN with various attribute embedding techniques, i.e., variants of graph neural networks, and compare EPE with different attribute fusing methods, i.e., adding, concatenation, and weighted sum. (8) In Section 6.2.4, a detailed ablation study is conducted to verify the effectiveness of each module of BERD+. (
Tumors are one of the most common tumors in humans, and the main treatment at present is surgical removal. Preoperative planning of tumors usually requires a professional doctor to manually segment the images, but thi...
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In light of the far-reaching impact of the COVID-19 pandemic, the accurate estimation of infected cases, fatalities, and recoveries has become crucial. While much research has focused on national-level predictions, it...
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Recombinant protein production in Escherichia coli (E. coli) is crucial for biotechnological applications, but optimizing protein titer is challenging and often requires iterative experimentation and expert guidance. ...
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ISBN:
(数字)9798350362480
ISBN:
(纸本)9798350362497
Recombinant protein production in Escherichia coli (E. coli) is crucial for biotechnological applications, but optimizing protein titer is challenging and often requires iterative experimentation and expert guidance. This study utilizes advanced filtering techniques—Kalman, particle, bilateral, and extended Kalman filters—to enhance titer estimation accuracy using fermentation data provided by Cytovance Biologics. The proposed techniques effectively impute missing titer values and are validated on a secondary dataset from Chinese Hamster Ovary (CHO) cell lines, demonstrating robustness and accuracy. By reducing the need for extensive sampling and iterative experimentation, these filtering techniques enable predictive control over fermentation conditions. One significant cost-saving advantage is identifying the protein titer plateau, allowing lab scientists to conclude the fermentation process at the optimal time. This approach ensures maximum protein production while preventing unnecessary resource consumption. As a result, this method significantly lowers the overall cost of recombinant protein production in E. coli while enhancing process efficiency.
Ischemic stroke(IS)is a prevalent neurological disorder often resulting in significant disability or ***,extracted from Polygonum cuspidatum *** Zucc.(commonly known as Japanese knotweed),has been recognized for its p...
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Ischemic stroke(IS)is a prevalent neurological disorder often resulting in significant disability or ***,extracted from Polygonum cuspidatum *** Zucc.(commonly known as Japanese knotweed),has been recognized for its potent neuroprotective ***,the neuroprotective efficacy of its derivative,(E)-4-(3,5-dimethoxystyryl)quinoline(RV02),against ischemic stroke remains inadequately *** study aimed to evaluate the protective effects of RV02 on neuronal ischemia-reperfusion injury both in vitro and in *** research utilized an animal model of middle cerebral artery occlusion/reperfusion and SH-SY5Y cells subjected to oxygen-glucose deprivation and reperfusion to simulate ischemic *** findings demonstrate that RV02 attenuates neuronal mitochondrial damage and scavenges reactive oxygen species(ROS)through mitophagy ***,Parkin knockdown was found to abolish RV02's ability to activate mitophagy and neuroprotection in *** results suggest that RV02 shows promise as a neuroprotective agent,with the activation of Parkin-mediated mitophagy potentially serving as the primary mechanism underlying its neuroprotective effects.
Diagnosability is an important parameter to measure the fault tolerance of a multiprocessor system. If we only care about the state of a node, instead of doing the global diagnosis, Hsu and Tan proposed the idea of lo...
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Shared decision making has become a crucial solution to build a consolidated healthcare system. While there is some research in the healthcare literature discussing the advantages and disadvantages of shared decision ...
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Neuroinflammation,mediated by the nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing-3(NLRP3)inflammasome,is a significant contributor to the pathogenesis of neurodegenerative disea...
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Neuroinflammation,mediated by the nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing-3(NLRP3)inflammasome,is a significant contributor to the pathogenesis of neurodegenerative diseases(NDDs).Reynos-in,a natural sesquiterpene lactone(SL),exhibits a broad spectrum of pharmacological effects,suggesting its potential therapeutic ***,the effects and mechanism of reynosin on neuroinflammation remain *** current study explores the effects and mechanisms of reynosin on neuroinflammation using mice and BV-2 microglial cells treated with lipopolysaccharide(LPS).Our findings reveal that reynosin effectively reduces microglial inflammation in vitro,as demonstrated by decreased CD11b expression and lowered interleukin-1 beta(IL-1β)and interleukin-18(IL-18)mRNA and protein ***,in vivo,results showed a re-duction in the number of Iba-1 positive cells and alleviation of morphological alterations,alongside decreased expressions of IL-1βand *** analysis indicates that reynosin inhibits NLRP3 inflammasome activation,evidenced by reduced transcription of NLRP3 and caspase-1,diminished NLRP3 protein expression,inhibited apoptosis-associated speck-like protein containing a CARD(ASC)oli-gomerization,and decreased caspase-1 ***,reynosin curtailed the activation of nicotinamide adenine dinuc-leotide phosphate(NADPH)oxidase,demonstrated by reduced NADP^(+)and NADPH levels,downregulation of gp91^(phox) mRNA,pro-tein expression,suppression of p47^(phox) expression and translocation to the ***,reynosin exhibited a neuroprotective effect against microglial inflammation in vivo and in *** collective findings underscore reynosin’s capacity to mitigate mi-croglial inflammation by inhibiting the NLRP3 inflammasome,thus highlighting its potential as a therapeutic agent for managing neuroinflammation.
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