Object pose refinement is essential for robust object pose estimation. Previous work has made significant progress towards instance-level object pose refinement. Yet, category-level pose refinement is a more challengi...
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With the extension of the service time of the structure and the worse service temperature conditions, the drift problem of high-temperature strain gauges gradually becomes prominent. This research aims to address the ...
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Aerobic glycolysis,also known as the Warburg effect,is a hallmark of cancer cell glucose metabolism and plays a crucial role in the activation of various types of immune *** 3-phosphate dehydrogenase(GAPDH)catalyzes t...
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Aerobic glycolysis,also known as the Warburg effect,is a hallmark of cancer cell glucose metabolism and plays a crucial role in the activation of various types of immune *** 3-phosphate dehydrogenase(GAPDH)catalyzes the conversion of D-glyceraldehyde 3-phosphate to D-glycerate 1,3-bisphosphate in the 6th critical step in *** exerts metabolic flux control during aerobic glycolysis and therefore is an attractive therapeutic target for cancer and autoimmune ***,GAPDH inhibitors were reported to function through common suicide inactivation by covalent binding to the cysteine catalytic residue of ***,by developing a high-throughput enzymatic screening assay,we discovered that the natural product 1,2,3,4,6-penta-O-galloyl-β-D-glucopyranose(PGG)is an inhibitor of GAPDH with Ki=0.5 μ*** blocks GAPDH activity by a reversible and NAD+and Pi competitive mechanism,suggesting that it represents a novel class of GAPDH ***-depth hydrogen deuterium exchange mass spectrometry(HDX-MS)analysis revealed that PGG binds to a region that disrupts NAD+and inorganic phosphate binding,resulting in a distal conformational change at the GAPDH tetramer *** addition,structural modeling analysis indicated that PGG probably reversibly binds to the center pocket of ***,PGG inhibits LPS-stimulated macrophage activation by specific downregulation of GAPDH-dependent glucose consumption and lactate *** summary,PGG represents a novel class of GAPDH inhibitors that probably reversibly binds to the center pocket of *** study sheds new light on factors for designing a more potent and specific inhibitor of GAPDH for future therapeutic applications.
Titanium alloys are widely used in various fields for its high strength, good corrosion resistance and high heat resistance. Many countries in the world have recognized the importance of titanium alloy materials, and ...
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The mechanical force between cells and the extracellular microenvironment is crucial to many physiological processes such as cancer metastasis and stem cell differentiation. Mitosis plays an essential role in all thes...
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The mechanical force between cells and the extracellular microenvironment is crucial to many physiological processes such as cancer metastasis and stem cell differentiation. Mitosis plays an essential role in all these processes and thus an in-depth understanding of forces during mitosis gains insight into disease diagnosis and disease treatment. Here, we develop a traction force microscope method based on monolayer fluorescent beads for measuring the weak traction force (tens of Pa) of mitotic cells in three dimensions. We quantify traction forces of human ovarian granulosa (KGN) cells exerted on the extracellular matrix throughout the entire cell cycle in three dimensions. Our measurements reveal how forces vary during the cell cycle, especially during cell division. Furthermore, we study the effect of paclitaxel (PTX) and nocodazole (NDZ) on mitotic KGN cells through the measurement of traction forces. Our results show that mitotic cells with high concentrations of PTX exert a larger force than those with high concentrations of NDZ, which proved to be caused by changes in the structure and number of microtubules. These findings reveal the key functions of microtubule in generating traction forces during cell mitosis and explain how dividing cells regulate themselves in response to anti-mitosis drugs. This work provides a powerful tool for investigating cell-matrix interactions during mitosis and may offer a potential way to new therapies for cancer.
In the process of mechanical analysis for equipment and pipe of reactor coolant system (RCS), due to the severe load condition, the stress analysis cannot satisfy the evaluation criteria at times, the limit analysis a...
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This paper presents a collaborative fall detection and response system integrating Wi-Fi sensing with robotic assistance. The proposed system leverages channel state information (CSI) disruptions caused by movements t...
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ISBN:
(数字)9798331509644
ISBN:
(纸本)9798331509651
This paper presents a collaborative fall detection and response system integrating Wi-Fi sensing with robotic assistance. The proposed system leverages channel state information (CSI) disruptions caused by movements to detect falls in non-line-of-sight (NLOS) scenarios, offering non-intrusive monitoring. Besides, a companion robot is utilized to provide assistance capabilities to navigate and respond to incidents autonomously, improving efficiency in providing assistance in various environments. The experimental results demonstrate the effectiveness of the proposed system in detecting falls and responding effectively.
This paper studies the problem of extracting planar regions in uneven terrains from unordered point cloud measurements. Such a problem is critical in various robotic applications such as robotic perceptive locomotion....
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When the vanadium battery was running, pump frequency and current density were two frequently adjusted parameters. When the pump frequency was constant, the main flow-electrical parameter variation needs to be further...
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Robotic applications across industries demand advanced navigation for safe and smooth movement. Smooth path planning is crucial for mobile robots to ensure stable and efficient navigation, as it minimizes jerky moveme...
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