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Highly efficient multiplex base editing:One-shot deactivation of eight genes in Shewanella oneidensis MR-1

Synthetic and systems Biotechnology 2023年第1期8卷 1-10页

作者： Yaru Chen Meijie Cheng Yan Li Lin Wang Lixia Fang Yingxiu Cao Hao Song Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(Ministry of Education) School of Chemical Engineering and TechnologyTianjin UniversityTianjin300072China Key Laboratory of Systems Bioengineering(Ministry of Education) Tianjin UniversityTianjin300072China

Obtaining electroactive microbes capable of efficient extracellular electron transfer is a large undertaking for the scalability of bio-electrochemical ***,researchers need to pursue the co-modification of multiple genes rather than expecting that modification of a single gene would make a significant contribution to improving extracellular electron transfer *** editing has enabled highly-efficient gene deactivation in model electroactive microbe Shewanella oneidensis *** multiplexed application of base editing is still limited by its low throughput procedure,we thus here develop a rapid and efficient multiplex base editing system in *** approaches to express multiple gRNAs were assessed firstly,and transcription of each gRNA cassette into a monocistronic unit was validated as a more favorable option than transcription of multiple gRNAs into a polycistronic ***,a smart scheme was designed to deliver one-pot assembly of multiple gRNAs.3,5,and 8 genes were deactivated using this system with editing efficiency of 83.3%,100%and 12.5%,*** offer some nonrepetitive components as alternatives genetic parts of sgRNA cassette,different promoters,handles,and terminators were *** multiplex base editing tool was finally adopted to simultaneously deactivate eight genes that were identified as significantly downregulated targets in transcriptome analysis of riboflavin-overproducing strain and control *** maximum power density of the multiplex engineered strain HRF(8BE)in microbial fuel cells was 1108.1 mW/m2,which was 21.67 times higher than that of the wild-type *** highly efficient multiplexed base editing tool elevates our ability of genome manipulation and combinatorial engineering in Shewanella,and may provide valuable insights in fundamental and applied research of extracellular electron transfer.

关键词： Multiplex gene editing Electroactive microorganisms Base editing Multiplexed engineering CRISPR Extracellular electron transfer

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An Attack and Defense Game Model with Constrained Strategies Based on Difficulty Levels 5

An Attack and Defense Game Model with Constrained Strategies...

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5th International Conference on Frontiers technology of information and Computer, ICFTIC 2023

作者： Ren, Jiaqi Liu, Jin Dong, Yibo Li, Zhe Li, Weili National University of Defense Technology Science and Technology on Information Systems Engineering Laboratory Changsha China

ISBN: (纸本)9798350309034

Recently, there has been a growing research interest in the field of infrastructure protection regarding the integration of complex networks and game theory. However, most studies have overlooked the varying difficulty levels when choosing different strategies, which does not align with scenarios in reality. Therefore, we propose a novel approach to analyze the interactions between attackers and defenders in infrastructure protection by imposing constraints on their strategies. Initially, we evaluate the difficulties of implementing strategies by considering the node degree in the network. Subsequently, we define three types of strategies: difficult strategies, moderate strategies, and easy strategies. Then we impose practical constraints on different types of strategies. Finally, we present a solution based on the strategy constraints and conduct experiments on a target network. The results highlight the unique characteristics displayed by decision-makers when establishing various constraints, as these constraints influence decision-makers selecting diverse strategies. By introducing strategy constraints into the defense and attack game model, we provide a realistic game perspective and offer new insights for studying the protection of infrastructure networks. © 2023 IEEE.

关键词： Complex networks Game theory Infrastructure protection Strategy constraintst

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Understanding the Thermal Impedance of Silicone Rubber/Hexagonal Boron Nitride Composites as Thermal Interface Materials

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Chinese Journal of Polymer science 2024年第3期42卷 352-363,I0008页

作者： Yuan Ji Shi-Da Han Hong Wu Shao-Yun Guo Feng-Shun Zhang Jian-Hui Qiu The State Key Laboratory of Polymer Materials Engineering Sichuan Provincial Engineering Laboratory of Plastic/Rubber Complex Processing TechnologyPolymer Research InstituteSichuan UniversityChengdu 610065China Institute of Chemical Materials China Academy of Engineering PhysicsMianyang 621900China Department of Mechanical Engineering Faculty of Systems Science and TechnologyAkita Prefectural UniversityAkita 015-0055Japan

Silicone rubber(SR) composites are most widely used as thermal interface materials(TIMs) for electronics heat dissipation. Thermal impedance as the main bottleneck limiting the performance of TIMs is usually neglected. Herein, the thermal impedance of SR composites loaded with different levels of hexagonal boron nitride(h-BN) as TIMs was elaborated for the first time by the ASTM D 5470 standard test and finite element analysis. It was found that elastic modulus and surface roughness of SR composites increased with the increase of h-BN content, indicating that the conformity was reduced. When the assembly pressure was 0.69 MPa, there existed an optimal h-BN content at which the contact resistance was minimum(0.39 K·cm^(2)·W^(-1)). Although the decreased bond line thickness(BLT) by increasing the assembly pressure was beneficial to reduce the thermal impedance, the proper assembly pressure should be selected to prevent the warpage of the contact surfaces and the increase in contact resistance, according to the compression properties of the SR composites. This study provides valuable insights into fabrication of high-performance TIMs for modern electronic device applications.

关键词： Thermal interface materials Hexagonal boron nitride Thermal impedance Surfaces

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Research on the Distribution of Judging Works in Large Scale Innovation Competitions Considering the Stratification of Experts' Preferences 9

Research on the Distribution of Judging Works in Large Scale...

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9th International Conference on Big Data and information Analytics, BigDIA 2023

作者： Sun, Jiaqi Guo, Chunxian Pan, Xiaogang Jiao, Yuanyuan Yin, Minglou Niu, Ge Laboratory National University of Defense Technology Science and Technology on Information Systems Engineering Changsha China

ISBN: (纸本)9798350330076

There are often no standard answers in large-scale innovation competitions. So it is hard to quantify the innovation, and Expert scoring is subjective. At the same time, under the premise of group decision-making, due to the large scale of the competition and the wide gap between the number of works and the number of experts, the random distribution of works will not only increase the pressure of evaluation but also greatly reduce the comparability of works' scores. All of these will lead to excessive errors in the evaluation of subsequent works. Given the above problems, this paper converts the comparability between the scores given by each work review expert into the comparability score and establishes the maximum comparability score model with the maximum comparability score as the goal. The model considers the constraints of experts' workload capacity (the number of works reviewed by each expert must be within a range) and the number of experts assigned to each work. According to the index, the large neighborhood search algorithm is used to obtain the final maximum comparability score and the optimal allocation scheme. The comparability score of the distribution scheme given in this paper is compared with the comparability score obtained by using the random distribution mode and the mode of PHOOMETHE. The results show that the work distribution scheme given in this paper is more comparable than the random distribution and PHOOMETHE. The results can ensure the fairness of the score of subsequent works to a greater extent. © 2023 IEEE.

关键词： Optimization

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Recent progress in carbene-catalyzed fluoroalkylation

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science China Chemistry 2024年第8期67卷 2448-2460页

作者： Bei Zhang Xuan Zhang Jian Wang Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control Collaborative Innovation Center of Atmospheric Environment and Equipment TechnologySchool of Environmental Science and EngineeringNanjing University of Information Science and TechnologyNanjing 210044China School of Pharmaceutical Sciences Tsinghua UniversityBeijing 100084China

The fluoroalkyl fragment plays a vital role in various fields such as pharmaceuticals and materials science, and has a significant impact on human life not only on the present but also in the future. The insertion of fluoroalkyl groups into the chemical skeletons can cause considerable alternations in the chemical, physical and biological properties of molecules, which has attracted numerous scientists from different fields. This review focuses on N-heterocyclic carbene(NHC)-catalyzed fluoroalkylation reactions, providing a detailed analysis and summary of the achievements in the past two decades. Many successful examples clearly indicate that NHCs can catalyze the tranformation of fluoroalkylation reagents with various electronic characteristics, whether electrophilic, nucleophilic, or radical precursors. Herein, the fluoroalkylation reactions are classified based on different catalytic modes of NHCs: Lewis base catalysis, Umploung catalysis, radical catalysis and others. Meanwhile, by refining and systematically studying the contribution of NHC catalysis in fluoroalkylation reactions, this review is expected to provide valuable insights and enlightenment for related fields.

关键词： N-heterocyclic carbene fluoroalkylation radical catalysis

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A Dynamic and Secure Join Query Protocol for Multi-User Environment in Cloud Computing

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IEEE Transactions on Cloud Computing 2025年第2期13卷 512-525页

作者： Li, Hongjun He, Debiao Feng, Qi Yang, Xiaolin Luo, Qingcai Wuhan University Key Laboratory of Aerospace Information Security and Trusted Computing Ministry of Education School of Cyber Science and Engineering Wuhan430072 China Wuhan University School of Cyber Science and Engineering Wuhan430072 China Qilu University of Technology Shandong Academy of Sciences Key Laboratory of Computing Power Network & Information Security Ministry of Education Shandong Computer Science Center Jinan250014 China National Key Laboratory of Security Communication Chengdu610041 China Inspur Cloud Information Technology Company Ltd. Shandong Jinan250000 China Inspur Academy of Science and Technology Shandong Jinan250101 China

The development of cloud computing needs to continuously improve and perfect the privacy-preserving techniques for the user’s confidential data. Multi-user join query, as an important method of data sharing, allows multiple legitimate data users to perform join query over the data owner’s encrypted database. However, some existing join query protocols may face some challenges in the practical application, such as practicality, security, and efficiency. In this article, we put forward a dynamic and secure join query protocol in the multi-user environment. Compared with some existing protocols, the proposed protocol has the following advantages. On the one hand, we utilize the dynamic oblivious cross tags structure to realize an efficient join query with forward and backward security. On the other hand, we combine the randomizable distributed key-homomorphic pseudo-random functions with join query to support multiple data users, which can provide resilience against the single user’s key leakage and resist collusion attacks between the cloud server and a subset of data users. We formally define and prove the security of proposed protocol. In addition, we give a detailed analysis of computation and communication overheads to demonstrate the efficiency of proposed protocol. Finally, we carry out some experimental evaluations to further demonstrate the superiority of functionality and efficiency. © 2013 IEEE.

关键词： Structured Query Language

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Research on Encryption technology in "Internet +" Big Data Security 9

Research on Encryption Technology in "Internet +" Big Data S...

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9th International Conference on Big Data and information Analytics, BigDIA 2023

作者： Yaoyu, Li Shuai, Jin Yue, Zhao National University of Defense Technology Science and Technology on Information Systems Engineering Laboratory Changsha China

ISBN: (纸本)9798350330076

In the context of the "Internet +"paradigm and the prevalence of big data, the issue of security pertaining to Internet information is becoming conspicuous. Conventional data analysis technology exhibits limited diagnostic efficiency and inadequate security performance, rendering it unsuitable for meeting the fundamental requirements of information security in the present era of big data. In considering the previous issues, by analyzing encryption technologies such as Twofish, Blowfish, and RSA, this study proposes a hybrid data encryption method by combining RSA and Blowfish algorithms, and a corresponding cloud computing environment based sensitive data sharing policy for efficiency-first scenarios. Experimental data demonstrates that the encryption efficiency and security performance of this hybrid encryption method exceed the baseline algorithm, and can ensure the encryption efficiency and security performance of data-sharing in cloud environment. © 2023 IEEE.

关键词： Cryptography

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Research on Security Management of Combat Data Based on Blockchain 9

Research on Security Management of Combat Data Based on Bloc...

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9th International Conference on Big Data and information Analytics, BigDIA 2023

作者： Zhao, Yue Li, Yaoyu Zhu, Jianghan National University of Defense Technology Science and Technology on Information Systems Engineering Laboratory Changsha China

ISBN: (纸本)9798350330076

In order to satisfy the security management requirements of combat data, such as availability, verifiability, and non-repudiation, it's necessary to establish a unified and standardized framework for identity authentication and security guarantee. This framework should address the shortcomings of traditional application model and ensure the business data integrity of combat data management system. The blockchain has the natural characteristic of multi-party and tamper proof, which would enhance the security, reliability, and credibility of combat data. Considering the high dynamic of the battlefield environment and the hierarchical mode of data analysis, we design a basic framework for combat data management based on blockchain. A trusted method of identity authentication is further proposed based on consensus mechanism. A protection mode of data security is constructed based on state secret protection protocol, which consists of signature data and encryption *** designed framework would support the long-term, stable, and efficient operation of the combat data management system. © 2023 IEEE.

关键词： Blockchain

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A Multi-target Path Planning Method for UAV Considering Distribution Constraint of Path Nodes 3rd

A Multi-target Path Planning Method for UAV Considering Dist...

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3rd International Conference on Autonomous Unmanned systems, ICAUS 2023

作者： Liu, Fugui Meng, Xiangxia Zhao, Yixin Nanjing University of Science and Technology JS Nanjing210094 China Science and Technology on Information Systems Engineering Laboratory JS Nanjing210007 China

ISBN: (纸本)9789819710829

The planning path of UAV needs to meet a variety of constraints, and the path planned by different weight combinations is also different. In order to reduce the influence of weight on the quality of path planning, this paper adopts NSGA-III algorithm for UAV path planning. Firstly, the task environment model of UAV is established, and the distribution constraint of path nodes are established to improve the quality of planning path. Secondly, NSGA-III algorithm is used for multi-objective path optimization, and the approximation to global optimization is realized by objective function normalization method and reference point mechanism. Finally, simulation experiments are carried out to verify the applicability of the algorithm. The experimental results show that the proposed algorithm can plan a variety of paths under the constraint conditions, and the algorithm has good convergence and high efficiency, which has certain practical significance in engineering. © Beijing HIWING Scientific and Technological information Institute 2024.

关键词： Motion planning

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Research on the Application of Swarm Intelligence Algorithms to the Fire Assignment 9

Research on the Application of Swarm Intelligence Algorithms...

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9th International Conference on Big Data and information Analytics, BigDIA 2023

作者： Yin, Minglou Pan, Xiaogang Jiao, Yuanyuan Li, Zhimeng National University of Defense Technology Science and Technology on Information Systems Engineering Laboratory Changsha China

ISBN: (纸本)9798350330076

The fire assignment problem is a multi-parameter, multi-constraint NP (non-deterministic polynomial)-complete problem, the swarm intelligence algorithm has an inherent advantage in the solution, especially in the genetic algorithm, ant colony algorithm and particle swarm algorithm is the most representative, but there are still some shortcomings, such as the lack of interpretability and the tendency to fall into local optimization, and this is the direction of the improvement of the strategy to improve the efficiency of the fire assignment, the intelligent decision-making is of great theoretical significance and practical application value. © 2023 IEEE.

关键词： Swarm intelligence

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