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Engineered AAV13 variants with enhanced transduction and confined spread

Zoological Research 2024年第4期45卷 781-790页

作者： Neng-Song Luo Yu-Xiang Cai Zeng-Peng Han Xiao-Kai Sui Wen-Jia Yuan Zi-Lian Zhang Hao-Dong Guo Jie Wang Kun-Zhang Lin Fu-Qiang Xu Wuhan National Laboratory for Optoelectronics Huazhong University of Science and TechnologyWuhanHubei 430074China Shenzhen Key Laboratory of Viral Vectors for Biomedicine Shenzhen-Hong Kong Institute of Brain ScienceShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong 518055China Key Laboratory of Magnetic Resonance in Biological Systems State Key Laboratory of Magnetic Resonance and Atomic and Molecular PhysicsNational Center for Magnetic Resonance in WuhanInnovation Academy for Precision Measurement Science and TechnologyChinese Academy of SciencesWuhanHubei 430071China Key Laboratory of Quality Control Technology for Virus-Based Therapeutics Guangdong Provincial Medical Products AdministrationNMPA Key Laboratory for Research and Evaluation of Viral Vector Technology in Cell and Gene Therapy Medicinal ProductsBrain Cognition and Brain Disease InstituteShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong 518055China University of Chinese Academy of Sciences Beijing 100049China Zhongnan Hospital Wuhan UniversityWuhanHubei 430072China Brain Case Biotechnology Co. Ltd.ShenzhenGuangdong 518107China College of Life Sciences Wuhan UniversityWuhanHubei 430072China Center for Excellence in Brain Science and Intelligence Technology Chinese Academy of SciencesShanghai 200031China

Precise targeting of specific regions within the central nervous system(CNS)is crucial for both scientific research and gene therapy in the context of brain ***-associated virus 13(AAV13)is known for its restricted diffusion range within the CNS,making it an ideal choice for precise labeling and administration within small brain ***,AAV13 mediates relatively low expression of target ***,we introduced specifically engineered modifications to the AAV13 capsid protein to enhance its transduction *** first constructed AAV13-YF by mutating tyrosine to phenylalanine on the surface of the AAV13 *** then inserted the 7m8 peptide,known to enhance cell transduction,into positions 587/588 and 585/586 of the AAV13 capsid,resulting in two distinct variants named AAV13-587-7m8 and AAV13-585-7m8,*** found that AAV13-YF exhibited superior in vitro infectivity in HEK293T cells compared to AAV13,while AAV13-587-7m8 and AAV13-585-7m8 showed enhanced CNS infection capabilities in C57BL/6 mice,with AAV13-587-7m8 infection retaining a limited spread *** modified AAV13 variants hold promising potential for applications in gene therapy and neuroscience research.

关键词： Adeno-associated virus 13 AAV13-YF AAV13-587-7m8 AAV13-585-7m8 transduction efficiency

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Calibration Approaches to Coupling Factor and Decoupling Attenuation for Coupling /Decoupling Networks (CDNs)

Calibration Approaches to Coupling Factor and Decoupling Att...

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International Conference on Electronic measurement and Instruments, ICEMI

作者： Zhiqiang Zhan Yibin Wan Yulong Teng Shanghai Key Laboratory of On-line measurement and control technology Shanghai Institute of Measurement and Testing Technology ShangHai China

ISBN: (纸本)9781665444927

This paper proposes a reliable CDNs Calibration method of Coupling Factor and Decoupling Attenuation applied to the field of Electromagnetic Compatibility Tests and Conducted Disturbances Included by Radio-Frequency FieldsImmunity Tests. Through comprehensive analysis, the definition and technical indicators of the CDNs'Coupling Factor and Decoupling Attenuation has been made at the first part of dissertation. Since a typical CDNcontains a $150\ \Omega$ balance port and a $50\ \Omega$ unbalance port, a standard $150\Omega/50\Omega$ adapter is necessary for calibrating the Coupling Factor and Decoupling Attenuation of CDNs. Furthermore, these standard instruments for calibration are also required that the Network Analyzer, coaxial precision loads, coaxial fixed attenuators and a reference ground. Meanwhile, this paper presents the standard instruments for calibration and technical indicators of them. Moreover, the evaluation of uncertainty measurement has been reviewed. Finally, the research has been applied to the technical measurement specification of China‐‐‐‐‐< >.

关键词： Couplings Radio frequency Uncertainty Network analyzers Attenuation Electromagnetic compatibility Calibration

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Topologically protected surface states in TaPdTe₅

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Quantum Frontiers 2022年第1期1卷 1-6页

作者： Lu, Qi Ran, Zhao Li, Yunlong Xu, Chenhang Hu, Jiayuan Yin, Xunqing Wang, Guohua Zhang, Wentao Luo, Weidong Xu, Xiaofeng Qian, Dong Key Laboratory of Artificial Structures and Quantum Control (Ministry of Education) Shenyang National Laboratory for Materials Science School of Physics and Astronomy Shanghai Jiao Tong University Shanghai 200240 China Key Laboratory of Quantum Precision Measurement of Zhejiang Province Department of Applied Physics Zhejiang University of Technology Hangzhou 310023 China Tsung-Dao Lee Institute Shanghai Jiao Tong University Shanghai 200240 China

By combining angle-resolved photoemission spectroscopy (ARPES) and first-principles calculations, we have studied the electronic band structure of a topological material candidate TaPdTe₅.Two inverted bulk gaps determined through the parity analysis were observed in TaPdTe₅ near the Fermi level, which causes the appearance of topologically protected surface states on the (010) surface. Beside the surface state, we also observed strong surface resonance state. The hybridization between the surface resonance state and the surface state changes the quasi-one-dimensional like Fermi surface sheets of the surface resonance state into two-dimensional Fermi pockets. Our findings provide comprehensive understanding about the interesting surface states in TaPdTe₅. © The Author(s) 2022.

关键词： ARPES Band inversion Band structure Surface state Topological order

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Large jitter amplitude calibration of SDH transmission analyzer by wide band FM method 8

Large jitter amplitude calibration of SDH transmission analy...

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8th International Symposium on Test Automation and Instrumentation, ISTAI 2020

作者： Zhan, Zhiqiang Yu, Lei Wang, Yunling Shanghai Institute of Measurement and Testing Technology Shanghai China Shanghai Key Laboratory of On-line Testing and Control Technology Shanghai China

ISBN: (纸本)9781839535635

Jitter is a very important parameter in wired digital communication. It is usually measured by SDH transmission analyzer. When the jitter of SDH transmission analyzer is calibrated, the Bessel function ratio method is used. However, the method is not suitable for large jitter amplitude output from SDH transmission analyzer such as 50UIpp above. This paper introduces the wide-band frequency modulation method to calibrate the jitter amplitude of SDH analyzer, and the uncertainty evaluation results are given at the end of this paper. © 2020 Institution of Engineering and technology. All rights reserved.

关键词： Calibration

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Numerical Study on Magnetic Field Characteristics of Electromagnetic Flowmeter with Small Excitation Module

Numerical Study on Magnetic Field Characteristics of Electro...

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作者： Xinhong Yao Xuejing Li Shanghai Institute of Measurement and Testing Technology Shanghai Key Laboratory of On-line Testing and Control Technology

Electromagnetic flowmeter（EMFM） plays an important role in many production processes such as industry, medicine and agriculture. According to the measurement principle, the traditional electromagnetic flowmeter must have a large-size excitation module to provide a uniform magnetic field. However, this structure limits the use of electromagnetic flow meters. The saddle coil design has certain applications in electromagnetic flow sensors. The geometric size of the saddle coil is smaller than the Helmholtz coil and other large coils, so it has certain advantages in practical applications. But it causes the inhomogeneity of the induced magnetic field, which affects the measurement accuracy. This paper analyzes a saddle-shaped excitation module. An extended theory with a small magnetized flowmeter is proposed and compared with the experimental results. The electromagnetic field solution of the finite element program is proposed. The results of this study have certain guiding significance for the design process of the electromagnetic flowmeter excitation coil.

关键词： Magnetic field Electromagnetic measure Characteristics simulation

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Deep Q-learning Sampling Based on Advantages 5

Deep Q-learning Sampling Based on Advantages

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5th International Conference on Intelligent Robotics and control Engineering, IRCE 2022

作者： Xie, Ming Ren, Xinrui Yu, Jianbo Shu, Feng Shanghai Engineering Research Center of Ultra-Precision Motion Control and Measurement Academy for Engineering and Technology Fudan University Shanghai China School of Optical-Electrical and Computer Engineering University of Shanghai for Science and Technology Shanghai China School of Microelectronics Fudan University State Key Laboratory of ASIC and System Shanghai200433 China

ISBN: (数字)9781665469951

ISBN: (纸本)9781665469951

Deep Q-learning (DQN) has shown recent success on a wide range of complicated sequential decision-making issues, especially in the classic control area. However, in most DQN training, the sampling policies, particularly the ϵ-greedy policy and its derivation rules, produce noise erroneously due to the unsteadiness and unreliability of Q values utilized for searching for optimum actions. We begin with a fundamental hypothesis: complete advantages-based exploitation may be employed to help in successful exploration, and the gap between advantages on outstanding and poor actions tends to extend during the early phases of training before stabilizing. We then present a novel DQN architecture with a superior sampling policy that is totally and directly based on advantage values. This new DQN is named A-sampling DQN, and it requires minor code changes based on the original DQN codes. In most cases, A-sampling DQN and its derivatives outperformed the traditional DQN method. © 2022 IEEE.

关键词： Deep learning

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Simulation analysis on magnetic field of internal linear motor in the micro compressor

Simulation analysis on magnetic field of internal linear mot...

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IEEE Advanced Information Management,Communicates,Electronic and Automation control Conference (IMCEC)

作者： Yibo Zhao Chenyun Li Shanghai Institute of Measurement and Testing Technology Shanghai China Shanghai Key Laboratory of Online Test and Control Technology Shanghai China

ISBN: (纸本)9781665479691

In this paper, the magnetic field of internal linear motor in the micro compressor is simulated. Through finite element analysis and calculation of the magnetic flux density within the internal air gap of the micro linear motor under the conditions of different air gap width, air gap thickness, magnetic steel thickness and overall radial size of the motor, it is found that the initial setting size is the best size, which can not only meet the premise that the magnetic induction strength is unchanged, but also ensure uniform magnetic field distribution, The size and quality of the compressor are minimized. On the other hand, this study also verifies the rationality of the scaling principle for miniaturization.

关键词： Magnetic flux density Induction motors Automation Computational modeling Air gaps Magnetic analysis Information management

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An Effective Hybrid Genetic Algorithm for the Multi-Robot Task Allocation Problem with Limited Span

SSRN

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SSRN 2024年

作者： Liu, Wenbo Kuang, Zhian Zhang, Yongcong Zhou, Bo He, Pengfei Li, Shihua School of Automation Southeast University Nanjing China Key Laboratory of Measurement and Control of Complex Systems of Engineering Ministry of Education Nanjing China Shanghai Friendess Electronic Technology Shanghai201100 China

Multi-robot task allocation is one of the most interesting multi-robot systems that have gained considerable attention due to various real-world applications. In this paper, we focus on a multi-robot task allocation problem where a set of industrial robots, which are installed on a gantry and have a limited working span, have to jointly perform a set of weld lines in large size. We present a mathematical model that captures the objective of minimizing the cycle time, i.e., the time required to complete a given set of weld lines. Due to the inherent difficulty of the problem, we propose an effective hybrid genetic algorithm to solve it. Because the robots are associated with a limited working span, the algorithm integrates a specific region division algorithm to divide the workpieces into a set of regions where the robots can reach all the weld lines in each region. It also incorporates a dedicated route-based crossover to generate promising offspring solutions and an effective neighborhoodbased local search procedure to improve each offspring solution as much as possible. Extensive experimental results on three benchmark instances show that the algorithm significantly outperforms reference methods. Additional experiments on real-world instances are presented to verify the algorithm’s effectiveness in solving the multi-robot task allocation problem with limited span. © 2024, The Authors. All rights reserved.

关键词： Industrial robots

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Study on A-GNSS Terminal Over-The-Air Test System

Study on A-GNSS Terminal Over-The-Air Test System

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International Conference on Electronic measurement and Instruments, ICEMI

作者： Yunling Wang Qing Cai Jie Xu Key Laboratory of On-line Testing and Control Technology Shanghai Institute of Measurement and Testing Technology Shanghai Shanghai China

ISBN: (纸本)9781665444927

The A-GNSS terminal Over-The-Air (OTA) test system is a system for OTA performance of terminals that support A-GNSS. Terminals supporting A-GNSS can search for satellite navigation signals quicker than terminals only supporting GNSS. Active test is to measure the radiant power and receiving sensitivity of terminal over the air in a microwave anechoic chamber, which displays radiation performance of whole terminal obviously. Many test plans for OTA have been released, and among them the most recognized one is released by CITA. The instruments are mainly provided by Spirent and R&S. This paper introduces test plans, test situation of network operators and test institutions, compares 2 solutions provided by Spirit and R&S, describes test procedures, and simulates sensitivity test process.

关键词： Microwave measurement Microwave integrated circuits Global navigation satellite system Anechoic chambers Sensitivity Satellites Power measurement

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Person Re-identification Lightweight Network Based on Progressive Attention Mechanism 6

Person Re-identification Lightweight Network Based on Progre...

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6th International Symposium on Autonomous Systems, ISAS 2023

作者： Shi, Chunlei Niu, Dan Gong, Hao Zhang, Mei Cao, Zhan Jin, Yulong Southeast University Nanjing China Southeast University Key Laboratory of Measurement and Control of Cse School of Automation Nanjing China Vision Technology Co. Ltd. Shanghai China Nanjing Agricultural University College of Sciences Nanjing China Co.Ltd Hangzhou China

ISBN: (纸本)9798350316155

This paper proposes a lightweight person re-identification network that incorporates a progressive attention mechanism The network aims to address the low accuracy issue in person re-identification caused by various factors such as different viewing angles, poses, illumination conditions, occlusions, and low image resolutions. Additionally, the network design takes into consideration the need for lightweight model deployment in practical scenarios. To improve the network's performance using limited training data, data augmentation techniques are employed to expand the training dataset and enhance the robustness of the network model. The Resnet-50 architecture serves as the backbone network, and a feature shunt structure with depthwise separable convolutions is introduced to reduce computational parameters and accelerate person retrieval inference speed. Furthermore, the feature extraction and embedding processes are separated, and a progressive attention module is introduced. This module gradually segments the features into local blocks of different granularity, allowing for the learning of discriminative features at each granularity level. This progressive approach enhances the network's ability to perceive foreground information from coarse to fine levels and improves feature matching capability. To supervise the model, a triplet loss function is utilized, specifically designed to address challenging samples. This loss function helps reduce intra-class variations while increasing inter-class separability. The efficacy of the proposed method in person re-identification is substantiated by conducting experimental evaluation on both the Market-1501 and DukeMTMC-ReID datasets. The experimental results demonstrate that the method achieves mAP indices of 88.1% and 79.1% on the respective datasets, providing strong evidence for its effectiveness in addressing the challenges of person re-identification. © 2023 IEEE.

关键词： Image resolution

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