检索结果-内蒙古大学图书馆

Robotic Intracellular Electrochemical Sensing for Adherent Cells

Cyborg and Bionic systems 2022年第1期2022卷 75-86页

作者： Weikang Hu Yanmei Ma Zhen Zhan Danish Hussain Chengzhi Hu Shenzhen Key Laboratoryof Biomimetic Robotisand Intlligent Systems Department of Mechanicaland Enery EngineeringSouthern University of Science and TechnologyShenzhenChina Department of Mechatronics Engineering National University of Sciences and TechnologyIslamabadPakistan Guangdong Provincial Key Laboratory of Human-Augmentation and Rehabilitation Robotics in Universities Southern Universityof Science and TechnologyShenzhenChina

Nanopipette-based observation of intracellular biochemical processes is an important approach to revealing the intrinsic char acteristics and heterogeneity of cells for better investigation of disease progression or early disease ***,the manual operation needs a skilled operator and faces problems such as low throughput and poor *** paper proposes an automated nanopipette-based microoperation system for cell detection,*** nonovershoot positioning of the nanopipette tip in proximity to the cell of interest,cell approaching and proximity detection between nanopipette tip and cell surface,and cell penetration and detection of the intracellular reactive oxygen species(ROS).A robust focus algorithm based on the number of cell contours was proposed for adherent cells,which have sharp peaks while retaining *** automated detection of adherent cells was evaluated on human umbilical cord vein endothelial cells(HUVEC)and NIH/3T3 cells,which provided an average of 95.65%true-positive rate(TPR)and 7.59%false-positive rate(FPR)for in-plane cell *** three dimensional nonovershoot tip positioning of the nanopipette was achieved by template matching and evaluated under the interference of *** current feedback was employed for the proximity detection between the nanopipette tip and cell ***,cell penetration and electrochemical detection of ROS were demonstrated on human breast cancer cells and zebrafish embryo *** work provides a systematic approach for automated intracellular sensing for adherent cells,laying a solid foundation for high-throughput detection,diagnosis,and classification of different forms of biochemical reactions within single cells.

关键词： diagnosis proximity retaining

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：