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hnRNP K regulates mitochondrial apoptosis induced by porcine circovirus type 3 capsid protein

Veterinary Microbiology 2025年 306卷 110557页

作者： He, Qing Cao, Siyu Yu, Wanting Li, Zhoumian Liu, Weijiao Tian, Chuanwen Li, Dantong Zou, Yawen Yu, Beilei Zhan, Yang Jiang, You wu, Jing Yang, Yi Wang, Naidong Hunan Provincial Key Laboratory of Protein Engineering in Animal Vaccines Laboratory of Functional Proteomics (LFP) & Research Center of Reverse Vaccinology (RCRV) College of Veterinary Medicine Hunan Agricultural University Changsha China College of Animal Science and Technology Jiangxi Agricultural University Jiangxi Nanchang China Key Laboratory of Animal Biosafety Risk Prevention and Control (North) P.R. China Shanghai Veterinary Research Institute Biosafety Research Center Chinese Academy of Agricultural Sciences Shanghai China

Porcine circovirus type 3 (PCV3) is a globally emerging circovirus affecting pigs and other animals. The capsid protein (Cap) is the sole structural protein of PCV, with a crucial role in virus evolution and pathogenesis. Through interactions with host factors, Cap enables viral entry, transport, and replication while modifying various cellular processes. Cap protein-induced apoptosis has important implications for viral pathogenesis, but remains poorly defined. Herein, we demonstrated for the first time that PCV3 Cap induced cell cycle arrest of PK-15 cells in S-phase and initiated apoptosis via a mitochondrial Caspase-9-dependent pathway. Truncation analysis localized the apoptotic determinant to the N-terminal 1–34 aa of PCV3 Cap and heterogeneous nuclear ribonucleoprotein K (hnRNP K) was identified as a host protein that binds to PCV3 Cap. Overexpression of hnRNP K reduced PCV3 Cap-induced release of Cyt-c into the cytoplasm, implying a regulatory role in apoptosis. Based on structural modelling and molecular docking, amino acids at sites 24 and 27 of Cap from PCV3 variants, which define genotypes (PCV3a/b/c), affected binding with hnRNP K. Specifically, PCV3c Cap (V24/K27 and V24/R27) had higher affinity than PCV3a Cap (A24/R27) or PCV3b Cap (A24/K27), consistent with its superior apoptosis-inducing capacity compared to PCV3a/b variants, highlighting the importance of Cap interactions with hnRNP K. In summary, we identified novel molecular determinants of PCV3 pathogenesis that will inform development of vaccines and diagnostics. © 2025 Elsevier B.V.

关键词： Cap HnRNP K Mitochondrial apoptosis Porcine circovirus type 3

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Deeply-Dyed Nanobead System for Rapid Lateral Flow Assay Testing of Drugs at Point-of-Care

SSRN

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SSRN 2022年

作者： Fan, Lingzhi Wu, Jianbing Yang, Jing Li, Fugang Yan, Wannian Tan, Fei Zhang, Madeline Draz, Mohamed S. Han, Huanxing Zhang, Pengfei Department of Central Laboratory Shanghai Skin Disease Hospital School of Medicine Tongji University Shanghai200443 China Key Laboratory of Drug Preventation and Control Technology of Zhejiang Province Zhejiang Police College Hangzhou China Key Laboratory of Biomedical Information Engineering of Ministry of Education School of Life Science and Technology Xi’an Jiaotong University Xi’an710049 China Shanghai Upper Biotech Co Ltd Shanghai201201 China Department of Medicine Case Western Reserve University School of Medicine ClevelandOH44106 United States Department of Chemistry and Chemical Biology Harvard University CambridgeMA United States Department of Pharmacy Changzheng Hospital the Second Military Medical University Shanghai200433 China

Point-of-care test (POCT) allows for rapid and sensitive screening of drugs of abuse. However, traditional gold nanoparticle-based lateral flow immunoassay strip is not sensitive enough for detection of trace drugs in hair samples. Herein, we aimed to develop a more specific system using a composite polymer nanobead that is deeply dyed with oil soluble dyes, and termed as deeply dyed nanobead (DDNB). The prepared composite nanobeads displayed a clear core-shell structure and the core/shell ratios were readily controlled by polymer/dye feeding ratios. The absorbance stable nanobeads capped with carboxyl groups were covalently conjugated with antibodies, and were employed for preparation of lateral flow immunoassay strips for sensitive detection of drugs in hair with naked eye. The developed drug strips allow the detection of drugs such as morphine and methamphetamine in hair samples within 13 min (including hair sample processing ~5 min). The cut-off value of DDNB strip for methamphetamine detection with naked eye is down to concentration of 8.0 ng/mL, which is about 3.1 times more sensitive than the traditional gold nanoparticles based lateral flow immunoassay. Moreover, the colorful DDNB system has the potential for multiplexing detection of analytes at point-of-care settings and low cost. © 2022, The Authors. All rights reserved.

关键词： Metal nanoparticles

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Simultaneous heterotrophic nitrification and aerobic denitrification (SND) for nitrogen removal: A review and future perspectives

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Environmental Advances 2022年 9卷

作者： Yan, Yiming Lu, Hongwei Zhang, Jin Zhu, Shuguang Wang, Yangqing Lei, Yu Zhang, Rui Song, Liyan Key Laboratory of Water Cycle and Related Land Surface Processes Institute of Geographic Sciences and Natural Resources Research Chinese Academy of Sciences Beijing 100101 China University of Chinese Academy of Sciences Beijing 100049 China Key Laboratory of Water Pollution Control and Waste Resource of Anhui Province College of Environment and Energy Engineering Anhui Jianzhu University Hefei 230601 China Environmental Microbiology and Ecology Research Center Chongqing Institute of Green and Intelligent Technology Chinese Academy of Science Chongqing 400714 China School of Resources and Environmental Engineering Anhui University Anhui 230039 China

The lack of fresh water has accelerated efforts to improve the current treatment technology for the reclamation of wastewater. Simultaneous heterotrophic nitrification and aerobic denitrification (known as SND) for nitrogen removal has drawn increasing attention due to its’ high efficiency and low cost. Comparing with traditional nitrogen removal, SND process can achieve simultaneous nitrification and denitrification within a reactor unit. Despite considerable efforts of SND strains isolation from various environment, SND application and its influencing parameters, the nitrogen removal mechanism are poorly elicited and represent major challenges in SND basic study and application. The review begins with an update on current state of knowledge on SND functioning strains isolation and is followed by SND engineering applications and the associated key impact parameters study. The advantages and shortcomings of SND engineering application are also concluded to provide theoretical and technical support for practical engineering applications optimization. The following topics are the highlights and discussion on the metabolic pathway and denitrification mechanism of SND, including the most recent discovery of a novel oxidase dnfA (oxidizes hydroxylamine to N₂) in SND strain, Alcaligenes sp. HO-1. Finally, the challenges of SND metabolic pathway and SND engineering application are recommended for future directions and the related strategies are proposed. Specifically, the paper proposed the study on SND nitrogen removal mechanism using multi-omics strategy. Understanding the SND nitrogen removal mechanism will help us to apply SND more efficiently for nitrogen removal from wastewater. © 2022 The Authors

关键词： Application Functioning SND strains Nitrogen removal pathway Perspective Simultaneous nitrification and denitrification

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Note on Interval Observer Design Based on Positive System Theory 6th

Note on Interval Observer Design Based on Positive System Th...

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6th International Conference on Positive Systems, POSTA 2018

作者： Guo, Shenghui Zhang, Wenyang Xu, Jingyun College of Electronics and Information Engineering Suzhou University of Science and Technology Suzhou215009 China Key Laboratory of Control Engineering of Henan Province Henan Polytechnic University Jiaozuo454000 China College of Engineering Huzhou University Huzhou313000 China

ISBN: (纸本)9783030043261

This paper surveys interval observer design methods based on positive system theory, and two cases by using linear transformation are considered: (1) time-invariant linear transformation, the main tool is Sylvester equation;(2) time-varying linear transformation, and the Jordan canonical form plays a key role. The conclusion is that the performance of the two methods can be optimized by modifying some matrices in the design process. A numerical example is given to show the validity of our results. © 2019, Springer Nature Switzerland AG.

关键词： Linear transformations

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Regret and cumulative constraint violation analysis for online convex optimization with long term constraints

arXiv

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arXiv 2021年

作者： Yi, Xinlei Li, Xiuxian Yang, Tao Xie, Lihua Chai, Tianyou Johansson, Karl H. School of Electrical Engineering and Computer Science and Digital Futures KTH Royal Institute of Technology Stockholm Sweden Department of Control Science and Engineering College of Electronics and Information Engineering Shanghai Research Institute for Intelligent Autonomous Systems Institute for Advanced Study Tongji University Shanghai China State Key Laboratory of Synthetical Automation for Process Industries Northeastern University Shenyang China School of Electrical and Electronic Engineering Nanyang Technological University Singapore Singapore

This paper considers online convex optimization with long term constraints, where constraints can be violated in intermediate rounds, but need to be satisfied in the long run. The cumulative constraint violation is used as the metric to measure constraint violations, which excludes the situation that strictly feasible constraints can compensate the effects of violated constraints. A novel algorithm is first proposed and it achieves an O(Tmax{c,1-c}) bound for static regret and an O(T (1-c)/2) bound for cumulative constraint violation, where c ϵ (0, 1) is a user-defined trade-off parameter, and thus has improved performance compared with existing results. Both static regret and cumulative constraint violation bounds are reduced to O(log(T )) when the loss functions are strongly convex, which also improves existing results. In order to achieve the optimal regret with respect to any comparator sequence, another algorithm is then proposed and it achieves the optimal O( p T (1 + PT )) regret and an O(√T) cumulative constraint violation, where PT is the path-length of the comparator sequence. Finally, numerical simulations are provided to illustrate the effectiveness of the theoretical results. Copyright © 2021, The Authors. All rights reserved.

关键词： Comparators (optical)

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Electrocatalytic Water Oxidation Activity-Stability Maps for Perovskite Oxides Containing 3d, 4dand 5dTransition Metals

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Angewandte Chemie 2023年第46期135卷

作者： Dr. Xiao Liang Prof. Wensheng Yan Yinglong Yu Kexin Zhang Wei An Prof. Hui Chen Prof. Yongcun Zou Prof. Xiao Zhao Prof. Xiaoxin Zou State Key Laboratory of Inorganic Synthesis and Preparative Chemistry College of Chemistry Jilin University 130012 Changchun China National Synchrotron Radiation Laboratory University of Science and Technology of China 230029 Hefei China Petrochemical Research Institute PetroChina 102206 Beijing China School of Materials Science and Engineering Key Laboratory of Automobile Materials of MOE State Key Laboratory of Automotive Simulation and Control Electron Microscopy Center Jilin University 130012 Changchun China

Improving catalytic activity without loss of catalytic stability is one of the core goals in search of low-iridium-content oxygen evolution electrocatalysts under acidic conditions. Here, we synthesize a family of 66 SrBO 3 perovskite oxides (B=Ti, Ru, Ir) with different Ti : Ru : Ir atomic ratios and construct catalytic activity-stability maps over composition variation. The maps classify the multicomponent perovskites into chemical groups with distinct catalytic activity and stability for acidic oxygen evolution reaction, and highlights a chemical region where high catalytic activity and stability are achieved simultaneously at a relatively low iridium level. By quantifying the extent of hybridization of mixed transition metal 3 d -4 d -5 d and oxygen 2 p orbitals for multicomponent perovskites, we demonstrate this complex interplay between 3 d -4 d -5 d metals and oxygen atoms in governing the trends in both activity and stability as well as in determining the catalytic mechanism involving lattice oxygen or not.

关键词： Electrocatalysis Hybridization Iridium Oxygen Evolution Reaction Perovskite

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Unravelling the biodegradation performance and mechanism of 3,6-dibromocarbazole by a novel strain Streptomyces sp. FW-32

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Journal of Water Process engineering 2025年 75卷

作者： Mi Feng Junjie Zhao Yanqin Lu Xuan Luo Xinghui Feng Yao Huang Zijun Chen Dongxia Fu Jianhua Chen College of Environmental Science and Engineering Guilin University of Technology Guilin 541004 China Guangxi Key Laboratory of Environmental Pollution Control Theory and Technology Guilin University of Technology Guilin 541006 China Engineering Research Center of Watershed Protection and Green Development University of Guangxi Guilin University of Technology Guilin 541006 China Guangxi Engineering Research Center of Comprehensive Treatment for Agricultural Non-Point Source Pollution Guilin University of Technology Guilin 541006 China Modern Industry College of Ecology and Environmental Protection Guilin University of Technology Guilin 541006 China Collaborative Innovation Center for Water Pollution Control and Water Safety in Karst Area Guilin University of Technology Guilin 541006 China Institute of Eco-environmental and Soil Sciences Guangdong Academy of Sciences Guangzhou 510650 China

Streptomyces sp. FW-32, an actinobacterial strain capable of degrading 3,6-dibromocarbazole (3,6-DBCZ), was isolated from polluted soils. >85 % of 3,6-DBCZ was degraded within 5 d under optimal conditions (glucose concentration of 5 g/L, pH of 7, initial 3,6-DBCZ concentration of 1 mg/L, temperature of 35 °C). The biodegradation of 3,6-DBCZ followed the first-order kinetic model with the degradation rate constant of 0.01713 h −1 . Biodegradation pathways of 3,6-DBCZ by Streptomyces sp. FW-32 were proposed, including debromination, oxidation, and aromatic ring cleavage. The intracellular enzymes of Streptomyces sp. FW-32, such as cytochrome P450, played an important role in 3,6-DBCZ biodegradation. In the earlier stage of 3,6-DBCZ biodegradation (1–2 d), the activities of SOD and CAT were enhanced by 3,6-DBCZ (≤10 mg/L), but 10 mg/L of 3,6-DBCZ inhibited the activities of these two antioxidant enzymes at the later period (4–6 days). Cell viability of strain FW-32 was stimulated by 3,6-DBCZ at 1 mg/L in the first two days of treatment, but it was suppressed by 10 mg/L of 3,6-DBCZ after 3 days of exposure. Strain FW-32 was able to efficiently degrade 3,6-DBCZ and its key intermediate carbazole in real river water. The results of this study illuminated the mechanism of 3,6-DBCZ biodegradation by a newly isolated strain FW-32 and indicated that strain FW-32 might be suitable for the elimination of 3,6-DBCZ from contaminated water. Overall, these findings were significant for developing microbial remediation to control 3,6-DBCZ pollution in water environments.

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The China groundwater crisis: A mechanistic analysis with implications for global sustainability

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Sustainable Horizons 2022年 4卷

作者： Lancia, Michele Yao, Yingying Andrews, Charles B. Wang, Xiaoli Kuang, Xingxing Ni, Jinren Gorelick, Steven M. Scanlon, Bridget R. Wang, Yanxin Zheng, Chunmiao State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control School of Environmental Science and Engineering Southern University of Science and Technology Shenzhen China EIT Institute for Advanced Study Ningbo China Department of Earth and Environmental Sciences School of Human Settlements and Civil Engineering Xi'an Jiaotong University Xi'an China S.S. Papadopulos & Associates Inc. RockvilleMD United States Department of Civil Engineering The University of Hong Kong Hong Kong Hong Kong College of Environmental Sciences and Engineering Peking University Beijing China Department of Earth System Science Stanford University StanfordCA United States Bureau of Economic Geology Jackson School of Geosciences University of Texas at Austin AustinTX United States China University of Geosciences Wuhan China

Over the past several decades, China has rapidly depleted groundwater storage, and has lagged in managing and protecting this resource. As a result, dried up rivers, land subsidence, saltwater intrusion, and wetland losses are extensive. To assist with managing this vital groundwater resource, we constructed the first national-scale numerical groundwater flow model of China to provide a comprehensive overview and understanding of the subsurface flow dynamics. The groundwater model is three-dimensional with a 10 km by 10 km horizontal resolution and five discretized vertical layers (up to 1700 m below the land surface). The calibrated model matched calculated to observed water levels and sufficiently reproduced water budgets. The model was used to reconstruct groundwater depletion by retracing the different stages of Chinese economic development. From 1945 to 2020, the northern provinces are characterized by an average groundwater depletion of ∼16.7 km3/yr (equivalent to 30% of the Yellow River's annual flow) while southern China has experienced no substantial groundwater depletion. Groundwater depletion is consistent with a large observed reduction in groundwater discharge as baseflow to the Yellow River (-23%) relative to negligible variation in baseflow in the Yangtze River. Our study provides a holistic tool to support development of nationwide water management strategies and temper the effects of a future water crisis that China's growing economy could propagate at the global scale. © 2022

关键词： Groundwater resources

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Fuzzy Rate Analysis of Operators and its Applications in Linear Spaces

Research Square

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Research Square 2021年

作者： Zhang, Yijin Li, Honggang Jin, Maoming Lin, Zongbing Nanjing Changjiang Waterway Engineering Bureau China Key Lab of Intelligent Analysis and Decision on Complex Systems Key Laboratory of Industrial Internet of Things and Networked Control Ministry of Education Chongqing University of Posts and Telecommunications Chongqing400065 China Pass College Chongqing Technology and Business University Chongqing401520 China Institute of Nonlinear Analysis Research Changjiang Normal University Chongqing400803 China School of Mathematics and Computer Science Panzhihua University Panzhihua617000 China

In this paper, a new concept, the fuzzy rate of an operator in linear spaces is proposed for the very first time. Some properties and basic principles of it are studied. Fuzzy rate of an operator B which is specific i... 详细信息

关键词： Membership functions

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Application of Iterative Learning control in X-Y Precision Planar Motion Platform

Application of Iterative Learning Control in X-Y Precision P...

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Chinese Automation Congress (CAC)

作者： Hongyu Yan Danping Jia Tao Zhang Hualiang Zhang Yingjie Xiong Xu Lu Yang Liu School of Artificial Intelligence Shenyang University of Technology Shenyang China School of Information Science and Engineering Shenyang University of Technology Shenyang China Key Laboratory of Networked Control Systems Chinese Academy of Sciences Shenyang China Shenyang Institute of Automation Chinese Academy of Sciences Shenyang China Institutes for Robotics and Intelligent Manufacturing Chinese Academy of Sciences Shenyang China School of Electrical Engineering Dalian University of Technology Dalian China College Of Information Science and Engineering Northeastern University Shenyang China

ISBN: (纸本)9781665426480

The size of the tracking error is one of the important indicators to measure the control system of the X-Y Precision Planar Motion Platform. Iterative learning control (ILC) can obtain higher tracking accuracy through multiple iterations. However, it is found in experiments that the tracking error of the XY precision motion stage using ordinary PD-type iterative learning will eventually fluctuate in a relatively range, and some high-frequency Noise will seriously affect the stability of the system after many iterations. Aiming at the above problems, a PD-type variable gain open-closed loop ILC with a low-pass filter is proposed. This control scheme can effectively remove high-frequency noise and reduce tracking errors. Finally, a hardware-in-the-loop real-time simulator is used to conduct a comparative experiment. Through comparison with PID controller and ordinary PD-type ILC, the experimental consequence show that this arithmetic has a smaller tracking error than the other two algorithms.

关键词： Tracking loops Tracking Low-pass filters control systems Size measurement Stability analysis Real-time systems

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