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Significant increase of assimilable organic carbon(AOC)levels in MBR effluents followed by coagulation,ozonation and combined treatments:Implications for biostability control of reclaimed water

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Frontiers of environmental Science & Engineering 2021年第4期15卷 241-249页

作者： Xiaojie Shi Zhuo Chen Yun Lu Qi Shi Yinhu Wu Hong-Ying Hu Environmental Simulation and Pollution Control State Key Joint Laboratory State Environmental Protection Key Laboratory of Microorganism Application and Risk Control(SMARC)School of EnvironmentTsinghua UniversityBeijing100084China Shenzhen Environmental Science and New Energy Technology Engineering Laboratory Tsinghua-Berkeley Shenzhen InstituteShenzhen518055China

As water reuse development has increased,biological stability issues associated with reclaimed water have gained *** study evaluated assimilable organic carbon(AOC)in effluents from a full-scale membrane biological reactor(MBR)plant and found that they were generally stable over one year(125-216µg/L),with slight increases in warmer *** additional tertiary treatments,the largest increases in absolute and specific AOCs were detected during ozonation,followed by coagulation-ozonation and ***,UV254 absorbance is known to be an effective surrogate to predict the AOC changes during *** coagulation prior to ozonation of MBR effluents for removal of large molecules was found to reduce the AOC formation compared with ozonation treatment ***,the results revealed that attention should be paid to seasonal variations in influent and organic fraction changes during treatment to enable sustainable water reuse.

关键词： Assimilable organic carbon(AOC) MBR effluents Tertiary treatments Coagulation Ozonation

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Attached cultivation of Scenedesmus ***1 on selected solids and the effect of surface properties on attachment

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Frontiers of environmental Science & Engineering 2019年第4期13卷 113-121页

作者： Hong-Ying Hu Tian-Yuan Zhang Xiao-Xiong Wang Yinhu Wu Guo-Hua Dao Environmental Simulation and Pollution Control State Key Joint Laboratory State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (MARC)School of EnvironmentTsinghua UniversityBeijing 100084China Shenzhen Environmental Science and New Energy Technology Engineering Laboratory Tsinghua-Berkeley Shenzhen InstituteShenzhen 518055China

Attached cultivation systems in the literature do not present a methodology to screen materials for microalgal growth. Hence, a method is needed to find suitable materials for attached cultivation that may enhance attachment of microalgae. In this paper, we have tested seven materials culturing Scenedesmus sp. LX1 (S. sp. LX1)to evaluate the attachment of microalgae on the material surface, its growth in suspension phase and the properties of the materials. Two materials showed attachment of S. sp. LX1, polyurethane foam and loofah sponge, and allowed microalgae to grow both in the surface of the material and suspended phase. Polyurethane foam proved to be a good material for attachment of S. sp. LX1 and the amount of attached microalgae obtained was 51.73 mg/L when adding 100 pieces/L. SEM images showed that the surface and the pore size of the materials affected the attachment of the microalgae, increasing its attachment in scaffold-like materials. Furthermore, the hydrophilic and hydrophobic properties of the materials also affected the attachment of microalgae. This research can be used as a methodology to search for the assessment of a material suitable for attachment of microalgae.

关键词： Attached cultivation Microalgae Material properties Polyurethane Scenedesmus

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Degradation of polyacrylamide (PAM) and methane production by mesophilic and thermophilic anaerobic digestion: Effect of temperature and concentration

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Frontiers of environmental Science & Engineering 2020年第6期14卷 75-85页

作者： Mona Akbar Muhammad Farooq Saleem Khan Ling Qian Hui Wang State Key Joint Laboratory of Environment Simulation and Pollution Control School of EnvironmentTsinghua UniversityBeijing 100084China Sino-Japan Friendship Center for Environmental Protection Beijing 100029China

Polyacrylamide(PAM)is generally employed in wastewater treatment processes such as sludge dewatering and therefore exists in the ***,it degrades slowly and can deteriorate methane yield during anaerobic digestion(AD).The impact or fate of PAM in AD under thermophilic conditions is still *** study mainly focuses on PAM degradation and enhanced methane production from PAM-added sludge during 15 days of thermophilic(55°C)AD compared to mesophilic(35°C)*** and PAM dose from 10 to 50 g/kg TSS were *** results showed that PAM degraded by 76%to 78%with acrylamide(AM)content of 0.2 to 3.3 mg/L in thermophilic ***,it degraded only 21%to 30%with AM content of 0.5 to 7.2 mg/L in mesophilic *** methane yield was almost 230 to 238.4 mL/g VSS on the 8th day in thermophilic AD but was 115.2 to 128.6 mL/g VSS in mesophilic *** investigation revealed that thermophilic AD with continuous stirring not only enhanced PAM degradation but also boosted the organics release from the sludge with added PAM and gave higher methane yield than mesophilic AD.

关键词： Polyacrylamide(PAM)degradation Acrylamide(AM) Mesophilic anaerobic digestion Thermophilic anaerobic digestion Methane production

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Plastic materials and water sources actively select and shape wastewater plastispheres over time

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Frontiers of environmental Science & Engineering 2022年第11期16卷 233-242页

作者： Yu Xia Xuyang Zhang Miao Zhang Liming Chen Xiaotong Tang Yuhong Sun Xiang Li School of Environmental Science and Engineering Southern University of Science and TechnologyShenzhen 518055China State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control School of Environmental Science and EngineeringSouthern University of Science and TechnologyShenzhen 518055China Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control School of Environmental Science and EngineeringSouthern University of Science and TechnologyShenzhen 518055China

The daily use of plastics presents a serious pollution issue due to their extremely slow *** and the biofilm that grows on plastics(i.e.,the plastisphere)are important subsets of plastic *** studies have been conducted to reveal the structures of the plastispheres,the driving factors for the formation of the plastisphere,and the ability of the plastispheres to degrade plastics in a variety of water ***,the plastispheres related to wastewater are *** this study,we used a microcosmic strategy to study the evolution of the plastispheres associated with microplastics(MPs)over time in *** found that plastic materials and water sources did not actively select and shape the plastispheres at an early stage,but the active selection for a unique niche of the plastisphere occurred after 14 d of *** addition,we confirmed that the alkB gene was densely present,and metagenomics showed some additional chemical reactions,which suggests that MPs are consumed by the microbes in the ***,metagenomics identified some metagenome-assembled genomes(MAGs)associated with high-density polyethylene(HDPE)and polyethylene terephthalate(PET).The identification of HDPE-associated MAGs and PET-associated MAGs further supports the notion that the selection for a unique niche of the plastisphere is driven by plastic materials and water sources(in this study,after 14 d of growth).Our discoveries bring new views on the behavior of the wastewater-associated plastisphere,especially how long it takes a wastewater plastisphere to form.

关键词： Microplastics Whole-genome metagenomic sequencing Plastisphere Plastic degradation MHETase

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Study on weak signal amplitude detection and circuit simulation based on a new chaotic system

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Chaos, Solitons and Fractals 2025年 198卷

作者： Zhao, Zhijie Yang, Ruyue Liu, Jing Liu, Guoqiang Institute of Electrical Engineering Chinese Academy of Sciences Beijing100190 China School of Electronic Electrical and Communication Engineering University of Chinese Academy of Sciences Beijing100049 China State Environmental Protection Key Laboratory of Simulation and Control of Groundwater Pollution Chinese Research Academy of Environmental Sciences Beijing100012 China

The filtering circuit is a crucial component in signal conditioning systems for noise reduction. However, its susceptibility to the measurement environment makes it challenging for signal conditioning circuits to process weak signals with high precision. In the context of flow signal detection using slurry-type electromagnetic flowmeters, the limited capability of traditional filter circuits to detect weak signals has led to difficulties in the measurement of small flow signals. To address this, in this study, we present a novel N-dimensional nonlinear dynamic system designed to outperform conventional filter circuits in weak signal detection. Under the excitation of weak low-frequency square wave signals, we verified the existence of chaotic states in the proposed system and analysed its chaos synchronisation. We proposed a method for detecting weak low-frequency square wave signals in the time domain using chaos synchronisation, thus overcoming the limitation of traditional chaotic systems, which are typically constrained to phase diagram analysis for weak signal detection. Numerical simulation and circuit simulation experiments were conducted, and the results showed that, in the absence of noise, the chaos synchronisation curve outputted by the new chaotic system demonstrates a linear relationship with the inputted weak square wave signal. The peak-to-peak values derived from the curve were used to accurately determine the amplitudes of weak square wave signals. A signal processing procedure was proposed to identify the amplitudes of weak signals under strong noise interference based on the peak-to-peak values outputted by the new chaotic system and two traditional analogue filters. Circuit simulation results showed that, under the influence of noise, the accuracy of the new chaotic circuit in linearly recognising the weak square wave amplitude was as high as 93.39 %, whereas those of the band-pass and low-pass filter circuits were only 81.76 % and 74.42 %, respe

关键词： Low pass filters

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Effect of ozone injection on the long-term performance and microbial community structure of a VOCs biofilter

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Journal of environmental Sciences 2018年第7期30卷 133-140页

作者： Prakit Saingam Zenab Baig Yang Xu Jinying Xi Environmental Simulation and Pollution Control State Key Joint Laboratory School of Environment Tsinghua University Key Laboratory of Microorganism Application and Risk Control of Shenzhen Graduate School at Shenzhen Tsinghua University

For biofilters treating waste gases containing volatile organic compounds（VOCs）, biomass accumulation is a common problem which will induce bed clogging and significant decrease in VOCs removal efficiency during long-term operation. In this study, ozone injection was developed as a biomass control strategy, and its effects on the biofilter performance and the microbial community structure were investigated in long-term operation. Two biofilters,identified as BF1 and BF2, were operated continuously for 160 days treating gaseous toluene under the same conditions, except that 200 mg/m^3 ozone was continuously injected into BF1 during days 45–160. During the operation period, ozone injection did not change the toluene removal efficiency, while the pressure drop of BF1 with ozone injection was significantly lowered compared with BF2. The wet biomass accumulation rate of BF1 was 11 g/m^3/hr, which was only46% of that in BF2. According to the carbon balance result, ozone injection also increased the toluene mineralization rate from 83% to 91%, which could be an important reason for the low biomass accumulation. The PMA-q PCR result indicated that ozone injection increased the microbial viability of the biofilm. The high-throughput sequencing result also revealed that the dominant phyla and genera were not changed significantly by ozone injection, but some ozonetolerant genera such as Rhodanobacter, Dokdonella and Rhodococcus were enhanced by ozone exposure. All the results verified that ozone injection is capable of sustaining the long-term performance of biofilters by lowering the biomass accumulation, increasing the microbial viability and changing the microbial community structure.

关键词： Biofilter Ozone VOCs Biomass control High-throughput sequencing

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Year-round changes in tropospheric nitrogen dioxide caused by COVID-19 in China using satellite observation

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Journal of environmental Sciences 2023年第10期132卷 162-168页

作者： Zhenyu Luo Hailian Xu Zhining Zhang Songxin Zheng Huan Liu State Key Joint Laboratory of ESPC State Environmental Protection Key Laboratory of Sources and Control of Air Pollution ComplexSchool of EnvironmentTsinghua UniversityBeijing 100084China

The lockdown policy deals a severe blow to the economy and greatly reduces the nitrogen oxides(NOx)emission in China when the coronavirus 2019 spreads widely in early *** we use satellite observations from Tropospheric Monitoring Instrument to study the year-round variation of the nitrogen dioxide(NO_(2))tropospheric vertical column density(TVCD)in *** NO_(2)TVCD reveals a sharp drop,followed by small fluctuations and then a strong rebound when compared to *** the end of 2020,the annual average NO_(2)TVCD declines by only 3.4%in Chinamainland,much less than the reduction of 24.1%in the lockdown *** the basis of quantitative analysis,we find the rebound of NO_(2)TVCD is mainly caused by the rapid recovery of economy especially in the fourth quarter,when contribution of industry and power plant on NO_(2)TVCD continues to *** revenge bounce of NO_(2)indicates the emission reduction of NOx in lockdown period is basically offset by the recovery of economy,revealing the fact that China’s economic development and NOx emissions are still not *** efforts are still required to stimulate low-pollution development.

关键词： COVID-19 Tropospheric NO2 TROPOMI Economy recovery Environment sustainability

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Concept and application of anaerobic suspended granular sludge bed （SGSB） reactor for wastewater treatment

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Frontiers of environmental Science & Engineering 2014年第5期8卷 797-804页

作者： Mingxia ZHENG Zhong YAN Jiane ZUO Kaijun WANG State Key Joint Laboratory of Environmental Simulation and Pollution Control School of Environment Tsinghua University Beijing 100084 China Beijing Municipal Research Institute of Environmental Protection Beijing 100037 China

Bed expansion serves an important function in the design and operation of an upflow anaerobic reactor. An analysis of the flow pattern of expanded granular sludge bed （EGSB） reactors shows that most EGSB reactors do not behave as expanded bed reactors, as is widely perceived. Rather, these reactors behave as fluidized bed reactors based on the classic chemical reactor theory. In this paper, four bed expansion modes, divided as static bed, expanded bed, suspended bed, and fluidized bed, for bioreactors are proposed. A high-rate anaerobic suspended granular sludge bed （SGSB） reactor was then developed. The SGSB reactor is an upflow anaerobic reactor, and its expansion degree can be easily controlled within a range to maintain the suspended status of the sludge bed by controlling upfiow velocity. The results of the full-scale reactor confirmed that the use of SGSB reactors is advantageous. The full-scale SGSB reactor runs stably and achieves high COD removal efficiency （about 90%） at high loading rates （average 40 kg-COD·m^-3·d^-1, maximum to 52 kg·COD·m^-3 ·d^-1） based on the SGSB theory, and its expansion degree is between 22% and 37%.

关键词： expansion characteristic high-rate bioreactor,anaerobic suspended granular sludge bed expandedgranular sludge bed reactor

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A global perspective on microbial risk factors in effluents of wastewater treatment plants

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Journal of environmental Sciences 2024年第4期138卷 227-235页

作者： Kejing Chen Jinsong Liang Yuhan Wang Yechen Tao Yun Lu Aijie Wang School of Civil and Environmental Engineering Harbin Institute of TechnologyShenzhen 518055China Shenzhen Guohuan Environmental Protection Technology Development Co. LTD.Shenzhen 518055China State Key Joint Laboratory of Environmental Simulation and Pollution Control State Environmental Protection Key Laboratory of Microorganism Application and Risk ControlSchool of EnvironmentTsinghua UniversityBeijing 100084China

Effective monitoring and management of microbial risk factors in wastewater treatment plants(WWTPs)effluents require a comprehensive investigation of these risks.A global survey on microbial risk factors in WWTP effluents could reveal important insights into their risk *** study aims to explore the abundance and types of antibiotic resistance genes(ARGs),virulence factor genes(VFGs),the vector of ARG/VFG,and dominant pathogens in global WWTP *** collected 113 metagenomes of WWTP effluents from the Sequence Read Archive of the National Center for Biotechnology Information and characterized the microbial risk *** results showed that multidrug resistance was the dominant ARG type,while offensive virulence factors were the most abundant type of *** most dominant types of ARGs in the vector of plasmid and phage were both aminoglycoside resistance,which is concerning as aminoglycosides are often a last resort for treating multi-resistant *** baumannii was the most dominant pathogen,rather than Escherichia coli,and a weak negative correlation between Escherichia coli and two other dominant pathogens(Acinetobacter baumannii and Bacteroides uniformis)suggests that using Escherichia coli as a biological indicator for all pathogens in WWTP effluents may not be *** Getah virus was the most dominant virus found in global WWTP *** study presents a comprehensive global-scale investigation of microbial risk factors in WWTP effluents,providing valuable insights into the potential risks associated with WWTP effluents and contributing to the monitoring and control of these risks.

关键词： Wastewater treatment plant Microbial risk factor Effluent Pathogen Antibiotic resistance gene Virulence factor gene

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Surface water and groundwater interactions contributed the seasonal NO 3 − and NH 4 + sources and transformations in a hilly region, China

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Journal of environmental Management 2025年 387卷

作者： Jun Wang Yuan Niu Chang Li Zian Liu Xia Jiang Yali Wu State Key Laboratory of Environmental Criteria and Risk Assessment National Engineering Laboratory for Lake Pollution Control and Ecological Restoration Beijing 100012 China State Environmental Protection Key Laboratory for Lake Pollution Control Chinese Research Academy of Environmental Sciences Beijing 100012 China College of Resources and Environmental Henan Agricultural University Zhengzhou 450046 China

Comprehensive understanding of nitrogen (N) sources and transformations under the interactions of variable water cycle processes were of great significance for formulating nitrogen mitigation strategies in aquatic systems. Multiple stable isotopes (δ 15 N-NO 3 - , δ 18 O-NO 3 - , δ 15 N-NH 4 + , δ 2 H-H 2 O, and δ 18 O-H 2 O) and hydrochemical characteristics in surface water (SW) and groundwater (GW) were to reveal seasonal NO 3 − and NH 4 + sources and transformations in a hilly region with frequent vertical movements in this study. The δ 2 H-H 2 O, δ 18 O-H 2 O, and hydrochemical analysis highlighted surface water and groundwater interactions. The correlation between δ 15 N-NO 3 - and d-excess demonstrated soil leaching. Nitrification was the predominant nitrogen transformation process in the watershed during both seasons although denitrification occurred in certain points. Primary source of NO 3 − in SW was soil nitrogen (SN; 34.5 ± 14.9 % in the dry season and 76.4 ± 8.1 % in the wet season). The contributions of various sources to NH 4 + in SW were relatively equal during the dry season, while GW (30.5 ± 19.3 %) and manure and sewage (M&S) (29.0 ± 15.2 %) were the primary sources during the wet season. This study proved that groundwater was a significant source for NO 3 − and NH 4 + input when utilizing source-track models, especially in hilly regions. These findings improve input sources for mixture models and identify the sources and transformations of water cycling and N biogeochemical processes.

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