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Genetically Engineered Bacteria for Mercury Detection and Its Application in environmental Monitoring

Genetically Engineered Bacteria for Mercury Detection and It...

环境安全与生态学基准/标准国际研讨会、中国毒理学会环境与生态毒理学专业委员会第三届学术研讨会、中国环境科学学会环境标准与基准专业委员会2013年学术研讨会

作者：汪用志 Dan Li Miao He Environmental simulation and pollution control(ESPC) State Key Joint Laboratory School of EnvironmentTsinghua University Environmental simulation and pollution control(ESPC)State Key Joint Laboratory School of EnvironmentTsinghua University

A mercury sensing element mer from marine Pseudomonas putida strain SP1 was used in recombinant luminescent bacteria *** using fusions of 512bp mer gene operon with prompterless luxCDABE in pUCD615plasmid,the recombinant luminescent bacteria showed highly specificity and sensitivity to *** limit of Hg was 0.33nM,and distinct luminescent could be detected in ***,Cu,Zn,Ca,Pb,,Mg,Mn,andAl did not interfere with the detection in a range of 10～*** a signal-on test,recombinant luminescent bacteria method encountered a major barrier of false negative *** introducing internal standard method in water sample monitoring,false negative rate of recombinant luminescent bacteria method would be under control,and would accelerated the practical application of recombinant luminescent bacteria method.

关键词： Genetically Engineered Bacteria for Mercury Detection and Its Application in environmental Monitoring

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Application of GIS Based Ecological Vulnerability Evaluation in environmental Impact Assessment of Master Plan of Coal Mining Area

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Procedia environmental Sciences 2013年 18卷 271-276页

作者： Xueqin Liao Wei Li Jinxiang Hou State Key Joint Laboratory of Environmental Simulation and Pollution Control School of Environment Beijing Normal UniversityBeijing 100875 China

Coal accounts for more than 70% of total energy consumption in China. Kinds of ecological problems resulting from coal mining have become increasingly serious, which directly threaten regional ecological security and people's normal production and livelihood in some areas. Ecological vulnerability is the overall performance of the ecosystem change under mining development, thus evaluation of ecological vulnerability based on GIS in Fuxin mining master plan environmental impact assessment was presented in this article. According to ecological condition of Fuxin, the ecological vulnerability index was established synthetically reflecting ecological environmental status, ecological sensitivity and landscape spatial structure, including 9 indictors. The study area was divided into 247 grids, then the EVI value of each grid was calculated by comprehensive evaluation and the results were divided into 5 levels by Zonal Statistic analysis of ArcGIS. Combining the distribution of vulnerability classification with the important ecological function area and natural reserves in the scope of planning, the research area was plotted into five types of zone, i.e., appropriate exploitation zone, optimized exploitation zone, moderate exploitation zone, restrictive exploitation zone and forbidden exploitation zone. Several adjustment suggestions were put forward to optimize the layout of Fuxin mining area and guide the ecological protection during coal exploitation.

关键词： Ecological Vulnerability Plan environmental Impact Assessment Fuxin mining area GIS Exploitation Zoning

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Inactivation and Regrowth of Antibiotic-resistant Bacteria by PAA Disinfection in the Secondary Effluent of a Municipal Wastewater Treatment Plant

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Biomedical and environmental Sciences 2013年第10期26卷 865-868页

作者： HUANG Jing Jing XI Jing Ying HU Hong Ying TANG Fang PANG Yu Chen State Key Joint Laboratory of Environmental Simulation and Pollution Control School of EnvironmentTsinghua University China PowerEngineering Consulting Group Corporation Graduate School at Shenzhen Tsinghua University

Inactivation and microbial regrowth of penicillin-, ampicillin-, cefalexin-, tetracycline-, chloramphenicol-, and rifampicin-resistant bacteria were studied to explore risks associated with selection and regrowth of antibiotic-resistant bacteria after PAA disinfection. The results showed that after exposure to 20 mg/L PAA for 10 min, inactivation of ampicillin-resistant bacteria reached 2.3-1og, which was significantly higher than that of total heterotrophic bacteria with a decrease of 2.0-log. In contrast, inactivation of tetracycline- resistant bacteria was significantly less efficient, reaching only 1.1-1og. Chloramphenicol-and tetracycline-resistant bacteria, as well as total heterotrophic bacteria regrew more than 10 fold compared to those in the untreated wastewater sample with 22 h stilling culture after exposure to 2 or 5 mg/L PAA as for 10 min.

关键词：氨苄西林多糖类细菌利福平氯霉素青霉素类四环素细菌消毒

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China's international trade and air pollution in the United states

China's international trade and air pollution in the United ...

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第八届全国优秀青年气象科技工作者学术研讨会

作者： Jintai Lin Da Pan Steven J.Davis Qiang Zhang Kebin He Can Wang David G.Streets Donald J.Wuebbles Dabo Guan Laboratory for Climate and Ocean-Atmosphere Studies Department of Atmospheric and Oceanic Sciences School of Physics Peking University Department of Earth System Science University of California Ministry of Education Key Laboratory for Earth System Modeling Center for Earth System Science Tsinghua University State Key Joint Laboratory of Environmental Simulation and Pollution Control School of Environment Tsinghua University Argonne National Laboratory Department of Atmospheric Sciences School of Earth Society and Environment University of Illinois at Urbana–Champaign Urbana IL 61801 water leeds School of Earth and Environment University of Leeds

China is the world's largest emitter of anthropogenic air pollutants, and measurable amounts of Chinese pollution are transported via the atmosphere to other countries, including the United states. However, a large fraction of Chinese emissions is due to manufacture of goods for foreign consumption. Here, we analyze the impacts of trade-related Chinese air pollutant emissions on the global atmospheric environment, linking an economic-emission analysis and atmospheric chemical transport modeling. We find that in 2006, 36% of anthropogenic sulfur dioxide, 27% of nitrogen oxides, 22% of carbon monoxide, and 17% of black carbon emitted in China were associated with production of goods for export. For each of these pollutants, about 21% of export-related Chinese emissions were attributed to China-to-US export. Atmospheric modeling shows that transport of the export-related Chinese pollution contributed 3–10% of annual mean surface sulfate concentrations and 0.5–1.5% of ozone over the western United states in 2006. This Chinese pollution also resulted in one extra day or more of noncompliance with the US ozone standard in 2006 over the Los Angeles area and many regions in the eastern United states. On a daily basis, the export-related Chinese pollution contributed, at a maximum, 12–24% of sulfate concentrations over the western United states. As the United states outsourced manufacturing to China, sulfate pollution in 2006 increased in the western United states but decreased in the eastern United states, reflecting the competing effect between enhanced transport of Chinese pollution and reduced US emissions. Our findings are relevant to international efforts to reduce transboundary air pollution.

关键词： China's international trade and air pollution in the United states

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The estimate of influence of drainage pipe network operation on influent of WWTP

The estimate of influence of drainage pipe network operation...

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International Conference on Pipelines and Trenchless Technology, ICPTT 2013

作者： Liu, Yanchen Qiu, Yong Jing, He Shi, Hanchang Li, Ji He, Renjie State Key Joint Laboratory of Environment Simulation and Pollution Control School of Environment Tsinghua University Beijing 100084 China School of Environmental and Civil Engineering Jiangnan Universtiy Wuxi 214000 China Wuxi Municipal Sewer System Company Wuxi 214000 China

ISBN: (纸本)9780784413142

The drainage pipe network plays a very important role that take on the collection and transportation of wastewater in sewer system. The well status of drainage pipe network is necessary to guarantee for the better operation efficiency of wastewater treatment plant. The study aims at the estimate the influence of drainage pipe network operation status on the influent of wastewater treatment plant(WWTP). The long-term and short-term and shock effect were investigated in the study. The result indicated that the influent wastewater component variation is obviously by the year and season rule in the long-term effect. The daily variation rule of wastewater quality and quantity is significant in the short-term *** shork effect is significant and the flow variation caused by rainfall became weak normally after 2-3 day of rain event. © ASCE 2013.

关键词： Rain

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Profile Distribution Characteristics of Total Nitrogen and Soil Organic Matter in Different Types of Land Use in Baiyangdian Lake

Profile Distribution Characteristics of Total Nitrogen and S...

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International Conference on Intelligent System Design and Engineering Application (ISDEA)

作者： Yong-Tao Zhang Bao-Shan Cui Yan Lan Zhen Han Ting-Ting Wang Yu Zhang Ya-Li Tong School of Environment State Key Joint Laboratory of Environmental Simulation and Pollution Control Beijing Normal University Beijing China

ISBN: (纸本)9781467348935

Baiyangdian Lake is one of the biggest freshwater wetland in North China. However, in recent years, Baiyangdian Lake was disturbed and utilized intensively, resulting in its land-use types changed greatly, which might change the characteristics of the nutrient element distribution. So profile distribution characteristics of soil organic matter (SOM) and total nitrogen (TN) were measured in four different land-use types in Baiyangdian Lake. Profile maps were constructed to describe the spatial distribution of SOM and TN. Results showed that spatial distributions of both SOM and TN were very similar in soil profiles(0-100cm) of the soils of woodland, farmland, and grassland, decreasing gradually with depth, while in the reed-bed soils, the spatial distribution characteristics in soil profiles was the opposite, increasing gradually with depth. Correlation analysis showed that the distribution of SOM and TN were influenced by pH values more strongly than influenced by TP contents.

关键词： Soil Nitrogen Lakes Correlation Mineralization Graphical models Distribution functions

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Mainland China's international trade and air pollution in the United states

Mainland China's international trade and air pollution in th...

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2014年海峡两岸气象科学技术研讨会

作者： Jintai Lin Da Pan Steven J.Davis Qiang Zhang Kebin He Can Wang David G.Streets Donald J.Wuebbles Dabo Guan Department of Earth System Science University of California Irvine Irvine CA 92697 U.S. Ministry of Education Key Laboratory for Earth System Modeling Center for Earth System Science Tsinghua University Beijing 100084 Mainland China State Key Joint Laboratory of Environment Simulation and Pollution Control School of Environment Tsinghua University Beijing 100084 Mainland China water@leeds School of Earth and Environment University of Leeds Leeds LS2 9JT UK State Key Joint Laboratory of Environment Simulation and Pollution Control School of Environment Tsinghua University Beijing 100084 Mainland China Argonne National Laboratory Lemont Illinois 60439 U.S. Department of Atmospheric Sciences School of Earth Society and Environment University of Illinois at Urbana-Champaign Urbana Illinois 61801 U.S. Ministry of Education Key Laboratory for Earth System Modeling Center for Earth System Science Tsinghua University Beijing 100084 Mainland China Collaborative Innovation Center for Regional Environmental Quality Beijing 100084 Mainland China Ministry of Education Key Laboratory for Earth System Modeling Center for Earth System Science Tsinghua University Beijing 100084 Mainland China

Mainland China is the world's largest emitter of anthropogenic air pollutants, and measurable amounts of Mainland Chins's pollution are transported via the atmosphere to other countries, including the United ***, a large fraction of Mainland Chinas emissions is due to manufacture of goods for foreign ***, we analyze the itnpacts of trade-related Mainland China's air pollutant emissions on the global atmospheric environment, linking an economic-emission analysis and atmospheric chemical transport *** find that in 2006, 36% of anthropogenic sulfur dioxide, 27% of nitrogen oxides, 22 % of carbon monoxide, and 17% of black carbon emitted in Mainland China were associated with production of goods for *** each of these pollutants, about 21% of export-related Mainland China's emissions were attributed to Mainland Chinato-US *** modeling shows that transport of the export-related Mainland China's pollution contributed 3-10% of annual mean surface sulfate concentrations and 0.5-1.5% of ozone over the western United states in *** Mainland China's pollution also resulted in one extra day or more of noncompliance with the US ozone standard in 2006 over the Los Angeles area and many regions in the eastern United *** a daily basis, the export-related Mainland China's pollution contributed, at a maximum, 12-24% of sulfate concentrations over the western United *** the United states outsourced manufacturing to Mainland China, sulfate pollution in 2006 increased in the western United states but decreased in the eastern United states, reflecting the competing effect between enhanced transport of Mainland China's pollution and reduced US *** findings are relevant to international efforts to reduce transboundary air pollution.

关键词： International trade Transboundary air pollution Consumption-based accounting Mainland China

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Genotoxicity Assessment and Clustering of Disinfection Byproducts by Using Recombinant Bacterial-array Sensors Specific for DNA Damage and Repair

Genotoxicity Assessment and Clustering of Disinfection Bypro...

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作者：李丹 Miao He Alexander M.Klibanov April Gu Department of Civil and Environmental Engineering Northeastern UniversityBostonMA02115U.S.A. Department of Chemistry Massachusetts Institute of TechnologyBostonMA02115U.S.A. Environmental simulation and pollution control(ESPC) State Key Joint Laboratory School of EnvironmentTsinghua University Environmental simulation and pollution control(ESPC)State Key Joint Laboratory School of EnvironmentTsinghua University

More than 600 DBPs have been identified in drinking water after disinfections, however,only less than 100 DBPs have been evaluated the genotoxicity and carcinogenicity, mainly because of lacking rapid,reliable,high-through screening *** report a novel approach to evaluate and predict the mode of genotoxic action of DBPs using a recombinant bacterial-array sensors specific for DNA damage and *** this,thirty-seven recombinant E. coli strains with different promoters were selected,which control the expression of genes associated with the known DNA damage and repair pathways in ***,including,reversal of damage,nucleotide excision repair,base excision repair,mismatch repair,recombinational repair, and error-prone *** demonstae the ability and confirm the validity of the proposed genotoxicity assay,one DNA damage model chemical Mitomycin C and seven DBPs,including Formaldehyde,Trichloroacetic Acid,Bromoacetic acid,lodoacetic acid,Trislon,NaBrO,and NaClO,were *** assay measures real-time gene expression level changes induced by the chemicals and the differential response patterns and its strength of these strains to various DBPs allowed classifying the chemicals based on potential genotoxic *** results are also compared to gentoxicity data obtained from conventional in vivo and in vitro geneotoxicy assays reported in the literate,and showed good *** also applied this recombinant bacterial sensor array to reclaimed water after disinfection,and the results showed it's a sensitive,flexible and high-through tool for environmental genotoxicity assessment and predication.

关键词： DNA Genotoxicity Assessment and Clustering of Disinfection Byproducts by Using Recombinant Bacterial-array Sensors Specific for DNA Damage and Repair

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Development of a novel conductance-based technology for environmental bacterial sensing

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Science Bulletin 2013年第Z1期 440-449页

作者： SHEN FangXia TAN MiaoMiao XU Hong XU ZhenCheng YAO MaoSheng State Key Joint Laboratory of Environmental Simulation and Pollution Control College of Environmental Sciences and Engineering Peking University College of Mechanical and Electrical Engineering Beijing Union University Department of Reproductive Health Guangdong Women and Children Hospital South China Institute of Environmental Science Ministry of Environmental Protection

In this study, a simple impedance based technology for measuring bacterial concentrations was developed. The measurement system includes the signal amplification, copper probes and a sample loader. During the experiments, the conductance of Bacillus subtilis var niger, Pseudomonas fluorescens, and Escherichia coli were measured using the combination of a pre-amplifier and a lock-in amplifier. The conductance data were modeled verses the bacterial concentrations. Results indicated that the relationship between the conductance of bacterial suspensions and their concentrations follows a generic model: Y=C1+C2×e ( X/C3 ) , where Y is the conductance (S), X is the bacterial concentration (Number/mL: abbreviated to N/mL) for all species tested, and C1 3 are constants. Gram negative P. fluorescens and E. coli assumed similar conductance curves, which were flatter than that of gram positive B. subtilis var niger. For P. fluorescens and E. coli the culturing technique resulted in higher concentration levels (statistically significant) from 2 to 4 times that measured by the impedance based technology. For B. subtilis var niger, both methods resulted in similar concentration levels. These differences might be due to membrane types, initial culturability and the obtained conductance curves. The impedance based technology here was shown to obtain the bacterial concentration instantly, holding broad promise in realtime monitoring biological agents.

关键词： bacterial impedance conductance bioaerosol bacterial concentrations

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Enhancing charge harvest from microbial fuel cells by controlling the charging and discharging frequency of capacitors

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Bioresource technology 2013年 146卷 812-815页

作者： Shiting Ren Xue Xia Lulu Yuan Peng Liang Xia Huang State Key Joint Laboratory of Environment Simulation and Pollution Control THU-VEOLIA Environment Joint Research Center for Advanced Environmental Technology School of Environment Tsinghua University Beijing 100084 PR China. State Key Joint Laboratory of Environment Simulation and Pollution Control THU-VEOLIA Environment Joint Research Center for Advanced Environmental Technology School of Environment Tsinghua University Beijing 100084 PR China. Electronic address: liangpeng@***. State Key Joint Laboratory of Environment Simulation and Pollution Control THU-VEOLIA Environment Joint Research Center for Advanced Environmental Technology School of Environment Tsinghua University Beijing 100084 PR China. Electronic address: xhuang@***.

Capacitor is a storage device to harvest charge produced from microbial fuel cells (MFCs). In intermittent charging mode, the capacitor is charged by an MFC first, and then discharged through an external resistance. The charge harvested by capacitor is affected by the charging and discharging frequency. In the present study, the effect of the charging and discharging frequency on charge harvest was investigated. At the switching time (ts) of 100 s, the average current over each time segment reached its maximum value (1.59 mA) the earliest, higher than the other tested conditions, and the highest COD removal (63%) was also obtained, while the coulombic efficiency reached the highest of 67% at the ts of 400 s. Results suggested that lower ts led to higher current output and COD removal, but appropriate ts should be selected in consideration of charge recovery efficiency.

关键词： Capacitor Charge harvest Charging and discharging frequency Coulombic efficiency Microbial fuel cell

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