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中国国际绿色化学研讨会(The 8th International Symposium on Green Chemistry in China(8th ISGCC'2007))

作者： Shaohua Lin Tian Li Qinju Sun State Key Lab of Pollution Control and Resource Reuse Tongji University Shanghai 200092 China Environmental Science Institute of Nanhai District Foshan 528200 China

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Isolation, identification of 1,2,4-trichlorobenzene-degrading strain pseudomonas nitroreducens J5-1 and cloning of chlorocatechol 1,2-dioxygenase gene

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Huanjing Kexue/environmental Science 2007年第8期28卷 1878-1881页

作者： Song, Lei Wang, Hui Jiang, Jian Gao, Jing-Si Shi, Han-Chang State Key Joint Laboratory of Environmental Simulation and Pollution Control Department of Environmental Science and Engineering Tsinghua University Beijing 100084 China

A bacterium capable of utilizing 1,2,4-trichlorobenzene as sole carbon source was isolated from the polluted soil sample. This baterium was identified as Pseudomonas nitroreducens according to its physiological and biochemical analysis and its 16S rDNA sequence (GenBank Accession ***107515). When the initial concentration of 1,2,4-TCB is 400 mg/L, J5-1 can achieve a maximum degradation rate of 90%. When the initial concentration of 1,2,4-TCB is 20 mg/L, the effect of degradation is the best. Degradation of 1,2,4-TCB by strain J5-1 obeys the first order dynamics. The total gene of chlorocatechol 1,2-dioxygenase was cloned from genomic DNA of J5-1.

关键词： 1,2,4-trichlorobenzene Biodegradation Chlorocatechol 1,2-dioxygenase Degradation dynamics Pseudomonas nitroreducens strain J5-1

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Function study on trichlorobenzene degradation by plasmid pT7

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Zhongguo Huanjing Kexue/China environmental Science 2007年第1期27卷 121-124页

作者： Song, Lei Wang, Hui Shi, Han-Chang State Key Joint Laboratory of Environment Simulation and Pollution Control Department of Environmental Science and Engineering Tsinghua University Beijing 100084 China

A degradation plasmid was drawn in Pseudomonas Stutzeri T7 using 1, 2, 4-trichlorobenzene (1, 2, 4-TCB) as the degradation bacteria of only carbon source. For studying the function of this plasmid, it was transformed in E. coli JM109, thus obtaining the ability to degrade 1, 2, 4-TCB. In the process of E. coli JM109 (containing plasmid pT7) degrading 1, 2, 4-TCB, the partial intermediate products were inspected and surveyed as 2, 3, 5-trichloromuconate, 2, 5-dichloro-carboxymethylenebut-2-en-4-olide, and 2, 5-dichloro-4-oxohex-2-enedioate respectively, adopting gas chromatography-mass spectrometry (GC-MS) technique. The enzyme activities of trichlorobenzene dioxygenase, dihydrodiol dehydrogenase, and catechol 1, 2-dioxygenase, the enzymes were inspected and surveyed respectively.

关键词： Degradation pathwaty Plasmid Pseudomonas Stutzeri Trichlorobenzene

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Effect of CH3OOH on the atmospheric concentration of OH radicals

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Progress in Natural Science:Materials International 2006年第11期16卷 1141-1149页

作者： WANG Caixia and CHEN Zhongming**(The state key laboratory of environmental simulation and pollution control, College of environmental Sciences, Peking University, Beijing 100871, China) [a]The state key Laboratory of Environmental Simulation and Pollution Control College of Environmental Sciences Peking University Beijing China

Methyl hydroperoxide （CH3OOH, MHP） is known to be a significant sink and reservoir of HOx and ROx radicals in the atmosphere. In order to investigate the impact of MHP on the concentration of atmospheric OH radicals... 详细信息

Methyl hydroperoxide （CH_{3OOH, MHP） is known to be a significant sink and reservoir of HO_{x and RO_{x radicals in the atmosphere. In order to investigate the impact of MHP on the concentration of atmospheric OH radicals, two key gas-phase reactions of MHP, i.e. the reactions with OH radicals and with UV photolysis, have been simulated at temperature of 293±2 K and total pressure of 1.01×105 Pa, using the long path Fourier transform infrared （LP-FTIR） spectrometry. OH radicals are generated by the photolysis of O3 in the presence of water vapor. Combined with the relative rate method, the reaction rate constant of MHP with OH radicals is determined to be （3.99±0.15）×10 -12 cm3·molecule -1 ·s -1 , and thus the atmospheric lifetime of MHP is estimated at 2.9 days. Furthermore, from detailed analysis of the UV photolysis of MHP, the yield of OH radicals is obtained to be 0.91±0.04. Based on the MHP atmospheric lifetime and the yield of OH radicals, it is concluded that MHP plays an essential role in the redistribution of OH radicals in the troposphere.}}}

关键词： OH radicals methyl hydroperoxide rate coefficient UV photolysis gas-phase reaction LP-FTIR.

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simulation and control strategy for the variational influent of WWTP

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Journal of environmental Sciences 2006年第1期18卷 62-68页

作者： LIU Yan-chen FAN Long SHI Han-chang WANG Zhi-qiang State Key Joint Laboratory of Environment Simulation and Pollution Control Department of Environmental Science and Engineering TsinghuaUniversity Beijing 100084 China

With the development of activated sludge model, the simulation software for the design and operation of wastewater treatment plant （WWTP） was produced and has been widely used. The dynamic change of the quality and flow of influent are major factors causing the unstable operation of wastewater treatment process. As a basic model, ASMI model was used for the simulation of activated sludge process, and double exponential model was selected for the simulation of secondary sedimentation tank. The influences of influent change to the aeration tank and secondary sedimentation tank were investigated, and the relationship among influent change, the quality of effluent and the level of sludge blanket in secondary sedimentation tank was established. On the basis of the simulation results, the operation of the WWTP could be adjusted under the dynamic change of the influent. Furthermore, the controlling strategy combined the feed-forward on the influent flow and the feedback on the level of sludge blanket in the secondary sedimentation tank was studied.

关键词： modeling dynamic simulation static simulation controlling strategy influent

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Distribution of key parameters in Carrousel oxidation ditch

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Zhongguo Huanjing Kexue/China environmental Science 2007年第6期27卷 792-796页

作者： Liu, Yan-Chen Fan, Long Wang, Zhi-Qiang Chen, Da-Fang Shi, Han-Chang Luo, Bin Wang, Ying-Zhe State Key Joint Laboratory of Environment Simulation and Pollution Control Department of Environmental Science and Engineering Tsinghua University Beijing 100084 China School of Chemical and Environmental Engineering China University of Mining and Technology Beijing 100083 China Guozhen Environmental Protection Company Hefei 230088 China

To enhance nitrogen removal, a systemic monitoring of the biological and hydrological parameters of Carrousel oxidation ditch in Changsha second wastewater treatment plant was carried out to study the feasibility of simultaneous nitrification and denitrification (SND). The variation and distribution of parameters such as flow velocity, the concentration of dissolved oxygen(DO) and mixed liquor suspended solids (MLSS) in oxidation ditch were measured and analysed, which were major control factors for SND. The dimensional distribution of flow velocity, DO and MLSS were affected markedly by the open condition of the fans, DO along the ditch changed obviously with time. DO and flow velocity were higher and the mixing of MLSS was sufficient when five fans were opened, while there was lower flow velocity ( less than or equal 0.15 m/s) in middle ditch when three fans were opened. The feasible operation conditions for SND were lower dissolved oxygen concentration, lower flow velocity and higher concentration of MLSS.

关键词： Carrousel oxidation ditch Dissolved oxygen MLSS Simultaneous nitrification and denitrification Velocity

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Decolorization of reactive brilliant red K-2BP by white rot fungus under sterile and non-sterile conditions

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Journal of environmental Sciences 2006年第3期18卷 428-432页

作者： GAO Da-wen WEN Xiang-hua QIAN Yi State Key Joint Laboratory of Environmental Simulation and Pollution Control Department of Environment Science and Engineering TsinghuaUniversity Beijing 100084 China.

Almost all the studies both domestic and international using white rot fungus for dye wastewater treatment are performed under sterile conditions. However, it is obviously unpractical that wastewater with dyes is treated under sterile conditions. A feasible study was made for using white rot fungus Phanerochaete chrysosporium to degrade reactive brilliant red K-2BP dye under non-sterile conditions. The results showed that there was no decolorizing effect under non-sterile condition if white rot fungus was incubated under non-sterile condition, and the decolorization was always near to 0% during decolorizing test for 3 d; in the meantime, a lot of yeast funguses were found in liquid medium when white rot fungus was incubated under non-sterile conditions; however, if white rot fungus was incubated under sterile condition firstly, its decolorization was above 90% under non-sterile condition, which was similar to that of sterile condition. So we point out that the treating process for wastewater with dyes should be divided into two stages. The first stage is that white rot fungus should be incubated under sterile conditions, and the second stage is that reactive brilliant red K-2BP is decolorized under non-sterile conditions. The method not only save the operation cost which decolorizing reactive brilliant red K-2BP under sterile condition, but also provide the feasibility for using white rot fungus to degrade wastewater with dyes under non-sterile conditions.

关键词： white rot fungus Phanerochaete chrysosporium reactive brilliant red K-2BP decolorization non-sterile condition

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Heterogeneous reaction of NO_(2) on the surface of NaCl particles

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Science China Chemistry 2006年第4期49卷 371-378页

作者： LI Hongjun ZHU Tong DING Jie CHEN Qi XU Bingye State Key Joint Laboratory of Environment Simulation and Pollution Control College of Environmental SciencePeking UniversityBeijing 100871China

The heterogeneous reaction of NO_(2)on NaCl particles has been investigated with the new sample preparation and the mode of gas-solid free *** Reflectance Infrared Fourier Transform Spectroscopy(DRIFTS)is used to characterize the adsorbed products in situ,combined with Ion Chromatographic(IC),X-ray Photoelectron Spectroscopy(XPS)and Scan Electron Micros-copy(SEM).Our results indicate that the reaction is not limited to the surface of the NaCl particles,but penetrated into the upper *** reactive sites determine the *** measurements show that nitrate formation on sodium chloride is second order in NO_(2)concentration and reactive uptake coefficient is(1.54±0.70)×10^(-5).

关键词： DRIFTS gas-solid diffusion heterogeneous reaction reaction order reactiveuptake coefficient

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Characteristics of wetland functional degradation and its ecological water requirement for restoration in Yilong Lake of Yunnan Plateau

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Chinese Science Bulletin 2006年第B11期51卷 127-135页

作者： CUI Baoshan ZHAI Hongjuan State Key Joint Laboratory of Environmental Simulation and Pollution Control School of Environment Beijing Normal University Beijing 100875 China

The wetland functional degradation can be described as the weakening orunbalance of the wetland functions under natural interference or active jamming, which makes thewetland unable to perform its unique functions or causes the weakening of these functions. By usingfuzzing mathematics, this paper studied the grades the wetland functions and calculated their indexvalues. By this quantitative method and based on the function index values, we analyzed the wetlandfunction degradation and calculated the water quantity required to meet different functional needsin each season... Here we put forth three graded restoration schemes by integrating the ecologicalwater requirement of different grades with the ecological restoration including water replenishing,water consumption and water pollution. According to the calculated model, the water level and waterquantity for the low scheme are 1414.53 m and 1.30X10~8 m~3 respectively; 1415.38 m and 1.78X10~8m~3for the middle sheme, and 416.87 m and 2.14X10~8 m~3 for the high scheme.

关键词：泛函降解泛函索引生态水用水需求水文学

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Experimental Study on Aero Conductivity of Porous Media

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Journal of China University of Geosciences 2006年第4期17卷 332-336页

作者：陈家军杨官光刘运武郑冰杨建 State Key Joint Laboratory of Environmental Simulation and Pollution Control School of Environment Beijing Normal University Beijing 100875 China Hegang Meteorological Bureau Hegang 154100 China

To study the variation pattern of aero conductivity of different porous media under low pressure conditions, three kinds of media are selected. These include sandy clay loam, fine sand, and medium sand, and air us fluid to conduct soil column ventilation tests. Pressure at both ends of the colruns is measured under different ventilation flow rates during testing. The test results show that the aero conductivity, solved by Darcy＇s law, is not a coustant. It is a variable, which increases first when air flow velocity is less than 0. 258 7 cm/s for sandy clay loam, 0. 637 3 cm/s for fine sand and then decreases when air flow velocity is bigger than that with the increase of the ventilation flow rate when the medium is determined. By analyzing various factors that influence the flow resistance, the reasons for variation in aero conductivity are found us follows： first, the change of pore structure results in better ventilation; second, the relatiouship hetwcen pressure head loss and air flow velocity is nonlinear, and it is beyond the condition of the Iminar flow domain to which Darcy＇ s law can he applied, when the air flow rate increases to a certain value and the flow velocity is in the transition range to turbulent flow.

关键词： aero conductivity Darcy＇ s law pressure head loss nonlinear.

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