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Improvement of photo-fermentative hydrogen production performance by expressing tetracycline resistance genes tetAR in Rhodobacter sphaeroides HY01

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INTERNATIONAL JOURNAL OF HYDROGEN ENERGY 2019年第30期44卷 15823-15832页

作者： Zheng, Xiaojing Ma, Hongyu Zhang, Jing Yan, Wei Yang, Honghui Xi An Jiao Tong Univ State Key Lab Multiphase Flow Power Engn Dept Environm Sci & Engn Xian 710049 Shaanxi Peoples R China Shaanxi Prov Land Engn Construct Grp Xian 710075 Shaanxi Peoples R China

A typical expression plasmid, pRK415, was widely used to introduce cloned DNA fragment into a broad range of Gram-negative bacteria, including Rhodobacter sphaeroides. Tetracy-cline (Tc) is required for stable inheritance of the plasmid, but it is subject to photooxidation, inhibitory to the growth of the host strain. However, in this study, the introduction of pRK415 into R. sphaeroides HY01 under tetracycline stress showed clear enhancement on H-2 production performance of the recombinant strain pRK415/HY01. The maximum H-2 production rate of pRK415/HY01 was enhanced by 10%-70% compared with the control group in the absent of Tc. Supplementing with Tc, the wild type strain HY01 showed repressed cell growth and reduced H-2 production performance. The tetAR genes knockout test demonstrated that the expression of tetAR genes on pRK415 promoted the H-2 production performance. And within tolerance concentration (Tc < 2.5 mg/L), higher Tc concentration led to higher H-2 production performance of pRK415/HY01. Expression of tetAR genes in the genome of *** HY01 by substituting partial of hupSL genes enhanced the H-2 production performance as well. The mechanism of the H-2 production performance enhancement was discussed. (C) 2018 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.

关键词： Rhodobacter sphaeroides pRK415 Hydrogen production Tetracycline resistance gene

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Laser-Synthesized Ligand-Free Cu Nanocatalysts in Electrochemical CO2 Reduction to Methane

arXiv

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arXiv 2025年

作者： Ye, Taiping Lactionov, Artyom Sozaev, Islam Popov, Anton Klimentov, Sergei Kabashin, Andrei V. Liu, Ya International Research Center for Renewable Energy State Key Laboratory of Multiphase Flow in Power Engineering Xi’an Jiaotong University Shaanxi 710049 China National Research Nuclear University MEPhI Moscow115409 Russia CNRS LP3 Aix-Marseille Université Marseille13288 France

Electrochemical CO2 reduction (eCO2R) represents a pivotal strategy for mitigating global carbon emissions while simultaneously converting renewable energy into storable chemical fuels. Copper-based catalysts have been extensively explored in this field due to their unique capability to catalyze multi-carbon products. However, the intrinsic complexity of eCO2R pathways on Cu surfaces often leads to mixed product distributions, posing a significant challenge for achieving high selectivity toward a single desired hydrocarbon. Herein, we report a breakthrough in methane selectivity using laser-synthesized, ligand-free Cu nanomaterials. Unlike conventional Cu catalysts that produce diverse products, these ligand-free nanoparticles exhibit unprecedented selectivity for methane (CH4) with a Faradaic efficiency (FE) exceeding 70% at superior overpotentials. The absence of surface ligands, a direct consequence of the ultrafast laser ablation synthesis, ensures abundant exposed active sites with tailored electronic and geometric configurations. We attribute the exceptional methane selectivity to the synergistic effects of active sites-rich surfaces and optimized *CO intermediate binding energetics, which favor the protonation pathway toward CH4 rather than C–C coupling. This work not only resolves the longstanding selectivity dilemma in Cu-catalyzed eCO2R but also establishes laser-synthesized ligand-free nanomaterials as a versatile platform for designing high-performance electrocatalysts. Copyright © 2025, The Authors. All rights reserved.

关键词： Nanoparticles

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Hydrodynamic mechanism study of the diameter-varying spray tower with atomization impinging spray

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SEPARATION AND PURIFICATION TECHNOLOGY 2021年 268卷 118608-118608页

作者： Wu, Xiaomei Sharif, Maimoona Yu, Yunsong Chen, Lei Zhang, Zaoxiao Wang, Geoff Xi An Jiao Tong Univ Sch Chem Engn & Technol 28 Xianning West Rd Xian 710049 Peoples R China Xi An Jiao Tong Univ State Key Lab Multiphase Flow Power Engn 28 Xianning West Rd Xian 710049 Peoples R China Univ Queensland Sch Chem Engn St Lucia Qld 4072 Australia

Our group proposed a diameter-varying spray tower and a new spray mode of dual-nozzle opposed impinging spray method to achieve the low-energy-consumption and environmentally-friendly CO2 capture process and some meaningful results have been obtained by experiments. However, the detailed information about the hydrodynamics (like liquid and gas phase distribution, gas-liquid contacting area and gas-liquid contacting time causing by impinging spray) inside the proposed tower is hard to be obtained by experimental work. In this work, to specifically explain the hydrodynamic mechanism with impinging spray inside the proposed spray tower, ANSYS Fluent18.0 and Euler-Lagrange approach were used to simulate the complex gas-liquid two-phase flow based on experimental results. The effects of nozzle location, nozzle number, spray angle, liquid injection velocity and gas intake mode were investigated, and the optimal design scheme of the spray tower was proposed. Simulation results indicated that the dual-nozzle opposed impinging spray method (DOSM) showed better absorption performance compared with the single-nozzle side spray method (SSSM) and single-nozzle middle spray method (SMSM). Spray angle of 60 degrees had efficient gas-liquid distribution. The liquid inlet velocity of 8 m?s? 1 was suggested under the experimental conditions. The double-sided gas intake mode intensified gas-liquid absorption performance. The simulation results were well verified by experiments. The relationship between flow field optimization and CO2 absorption performance enhancing was also discussed comprehensively. Finally, it was concluded that both the numerical simulation and experimental results proved that the diameter-varying spray tower with the impinging spray method is a promising reactor for the CO2 capture system.

关键词： Carbon dioxide capture flow field optimization Diameter-varying spray tower Atomization impinging spray

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Unprotected mothers and infants breastfeeding in public amenities during the COVID-19 pandemic

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ENVIRONMENTAL CHEMISTRY LETTERS 2020年第5期18卷 1447-1450页

作者： Wang, Xue Han, Jie Lichtfouse, Eric Xi An Jiao Tong Univ Dept Environm Sci & Engn Xian 710049 Shaanxi Peoples R China Aix Marseille Univ CNRS IRD INRAEColl FranceCEREGE F-13100 Aix En Provence France Xi An Jiao Tong Univ State Key Lab Multiphase Flow Power Engn Xian 710049 Shaanxi Peoples R China

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Prediction of the severe slugging period in gas-liquid two-phase pipeline-riser systems using an artificial neural network

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Energy 2025年 331卷

作者： Yingjie Chang Qiang Xu Bo Huang Xuemei Zhang Haiyang Yu Hao Chen Dominique Thévenin Liejin Guo Shaanxi Key Laboratory of New Transportation Energy and Automotive Energy Saving School of Energy and Electrical Engineering Chang'an University 710064 Xi'an China State Key Laboratory of Multiphase Flow in Power Engineering Xi'an Jiaotong University 710049 Xi'an China Laboratory of Fluid Dynamics and Technical Flows Otto-von-Guericke-Universität Magdeburg 39106 Magdeburg Germany

Severe slugging flow (SS) is a very harmful flow regime found during oil and gas transportation. An accurate prediction of the SS period would help to obtain the needed conditions for regime transition models, which are essential when designing and operating an oil and gas field. However, previous empirical correlations are limited to specific flow and structural conditions, resulting in significant errors whenever these parameters deviate. Gas-liquid two-phase flow experiments in a pipeline-riser system were carried out and used as additional training data. The analysis of scatter matrix and correlation coefficient was used to determine the most relevant features impacting SS period. Then, a prediction model based on an artificial neural network (ANN) was established with one single output (the SS period) and seven input features, including five pipeline structural parameters and two flow parameters. The prediction errors coming with previous empirical models are much larger than those of our ANN model. While the relative errors of the empirical models range from −95 % to 3000 %, the ANN has a prediction error within a range of 25 % and a mean absolute error of only 11.3 %, showing a significant improvement over previous predictions.

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Lithium Extraction from High Magnesium Salt Lake Brine with an Integrated Membrane Technology

SSRN

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SSRN 2022年

作者： Ashraf, Muhammad Awais Usman, Muhammad Hussain, Iftikhar Ahmad, Faraz Guo, Shiwei Zhang, Lu State Key Laboratory of Multiphase Complex Systems CAS Key Laboratory of Green Process and Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing100190 PR China University of Chinese Academy of Sciences Beijing100049 China Washington State University United States Department of Mechanical Engineering City University of Hong Kong 83 Tat Chee Avenue Kowloon Hong Kong Key Laboratory of Coal Science and Technology Taiyuan University of Technology Taiyuan030024 China North China Electric Power University Hebei Key Lab of Power Plant Flue Gas Multi-Pollutants Control Department of Environmental Science and Engineering Baoding071000 China Faculty of Economics and Business

Lithium extraction is a great challenge from the high magnesium content salt solution. In this work, an integrated polyamide tight ultrafiltration (UF) and nanofiltration (NF) membranes are systematically investigated to separate Li+ from a high magnesium salt solution. Tight ultrafiltration UA001 and nanofiltration NF270 membranes in the integrated-cascade system not only increased Li+ permeation to 82.5% but also enhanced the high Mg2+ retention to 94.6% from the ternary salt solution of LiCl and MgCl2. The transport model was also developed to calculate the real retention of Li+ and Mg2+, under constant pressure mode. The separation efficiency SFLi/Mg for the ternary salt solution of LiCl+MgCl2+H2O was more than 15 at a constant pressure of 25 bars with an integrated membrane process, which was higher as compared to single-stage UF and NF270 membranes. Moreover, the high Mg2+/Li+ ratio was also reduced from 30 to 1.9 in the integrated-cascade membrane process. © 2022, The Authors. All rights reserved.

关键词： Magnesium compounds

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Experimental and techno-economic analysis of two innovative solar thermal receiver designs for a point focus solar Fresnel collector

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ENERGY 2022年第PartA期261卷

作者： Danish, Syed Noman Al-Ansary, Hany El-Leathy, Abdelrahman Ba-Abbad, Mazen Khan, Salah Ud-Din Rizvi, Arslan Orfi, Jamel Al-Nakhli, Ahmed King Saud Univ Sustainable Energy Technol Ctr POB 800 Riyadh 11421 Saudi Arabia King Saud Univ Mech Engn Dept POB 800 Riyadh 11421 Saudi Arabia King Saud Univ KACARE Energy Res & Innovat Ctr Riyadh Saudi Arabia Helwan Univ Fac Engn Mech Power Engn Dept Cairo 11718 Egypt King Abdulaziz City Sci & Technol Riyadh Saudi Arabia Xi An Jiao Tong Univ State Key Lab Multiphase Flow Power Engn 28 Xianning West Rd Xian 710049 Shaanxi Peoples R China

In this paper, techno-economic analysis and the experimental campaign for a new solar concentrating system are presented. Two novel receivers (Jet impingement receiver with mesh structure & helical channel receiver) are tested on a novel collector technology. The collector technology integrates the two established models of solar concentrating technologies, which are linear Fresnel reflector technology and central receiver technology, into the new concept called the Point Focus Fresnel Collector (PFFC). It is found that the PFFC system with jet impingement receiver and mesh structure provides higher thermal efficiency and manufacturing ease compared to parabolic dish systems. Maximum thermal efficiency of jet impingement receiver is highest at 87% whereas that for helical channel receiver is 83%. Average thermal efficiency of jet impingement receiver is 61% whereas that for helical channel receiver is 58%. Economic analysis of the system reveals that the discounted payback time for the PFFC system is only 5.5 years and less than one year compared to diesel and electricity powered steam generators respectively at an inflation rate of 2.5%. Substantial saving is predicted with PFFC system compared to diesel and electricity powered steam generator. For all discount rates in the range of 2.5-25%, the levelized cost of energy (LCOE) for PFFC system is much lower than that of LCOE in Saudi Arabia for conven-tional system. Since the LCOE in most of the Middle East and North Africa (MENA) countries is higher than the LCOE in Saudi Arabia, therefore, the PFFC system is also suitable for other MENA countries where average direct normal irradiation values are comparable to Saudi Arabia.

关键词： Concentrated solar power Point focus Fresnel collector Economic analysis Experimentation Jet impingement receiver Helical channel receiver

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Flame front identification and its effect on turbulent premixed flames topology at high pressure

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EXPERIMENTAL THERMAL AND FLUID SCIENCE 2019年 107卷 107-117页

作者： Wang, Jinhua Chang, Min Zhang, Meng Li, Guohua Chen, Shuang Huang, Zuohua Xi An Jiao Tong Univ State Key Lab Multiphase Flow Power Engn Xian 710049 Shaanxi Peoples R China Northwest Inst Nucl Technol State Key Lab Laser Interact Matter Xian 710024 Shaanxi Peoples R China China Aerodynam Res & Dev Ctr Mianyang 621000 Sichuan Peoples R China

Image processing is of primary importance in the laser diagnostic on turbulent combustion, which can provide quantitative information, such as velocity field, intermediate species profile. Turbulent premixed flame front indicates the flame-turbulence interaction, can be decided from the OH-PLIF (Planar Laser Induced Fluorescence) technique due to the sharp increase of the OH distribution from unburned to burned region. In this paper, an adaptive threshold binarization method was proposed based on the local gray histogram of the OH-PLIF images when the signal-noise ratio is relatively low, i.e. turbulent flames at intensive turbulence and high pressure. The noise was eliminated by seed-mediated growth to obtain the legible flame front. Effect of different flame front identification methods, namely global binarization method, the canny operator of Matlab and the proposed adaptive threshold binarization method, on the flame structure parameters at various conditions was investigated. Results show that the flame fronts are seriously vague using the global binarization and the canny operator when the signal-noise ratio is low at intensive turbulence and high pressure, while continuous flame front can still be attracted by the proposed adaptive threshold binarization method. The turbulent flame front parameters from different methods are almost identical at weak turbulence and atmospheric pressure. While due to the noise introduced, the value of flame brush thickness, flame height and flame surface density from the proposed method is smaller than that of other two methods at high pressure. The adaptive threshold binarization method can obtain a better flame front identification at low signal-noise ratio conditions, results in reasonable premixed turbulent flame front parameters at intensive turbulence and high pressure.

关键词： Turbulent premixed flame Combustion laser diagnostics Flame front identification Flam front structure High pressure

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Determination of manganese in submerged steel using Fraunhofer-type line generated by long-short double-pulse laser-induced breakdown spectroscopy

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SPECTROCHIMICA ACTA PART B-ATOMIC SPECTROSCOPY 2021年 180卷 106210-106210页

作者： Cui, Minchao Deguchi, Yoshihiro Li, Guoxi Wang, Zhenzhen Guo, Haorong Qin, Zixiong Yao, Changfeng Zhang, Dinghua Northwestern Polytech Univ Key Lab High Performance Mfg Aero Engine MIIT Xian 710072 Peoples R China Northwestern Polytech Univ Engn Res Ctr Adv Mfg Technol Aero Engine MOE Xian 710072 Peoples R China Tokushima Univ Grad Sch Adv Technol & Sci Tokushima 7708501 Japan Xi An Jiao Tong Univ State Key Lab Multiphase Flow Power Engn Xian 710049 Peoples R China

The emission spectral lines of underwater laser-induced plasma (LIP) are often influenced by a strong selfabsorption effect. It is found that the long-short double-pulse laser-induced breakdown spectroscopy (LS-DPLIBS) is able to generate the Fraunhofer-type signal from the submerged solid samples. In this work, the manganese concentrations in submerged steel samples are quantitatively analyzed using the Fraunhofer-type signal. We firstly show the Fraunhofer-type signal generated by LS-DP-LIBS. The parameters of the Fraunhofer-type absorption lines are briefly presented and discussed. Then, the Fraunhofer-type line at Mn I 403.307 nm is used to calculate the optical density (OD), which is a parameter for evaluating plasma absorbance. A calibration curve is established using OD values and certified manganese concentrations of ten samples. The results suggest that there is a linear relationship between OD values and Mn concentrations. Therefore, we have further conducted two prediction tests based on the measurement results of ten samples. The results show that the relative error of prediction is less than 10%, which demonstrates that the Fraunhofer-type absorption lines are worthy of study in the quantitative measurement of submerged solid samples.

关键词： LIBS Long-short double-pulse Fraunhofer-type lines Quantitative analysis Submerged steel

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Effects of fuel composition and initial pressure on laminar flame speed of H2/CO/CH4 bio-syngas

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FUEL 2019年 238卷 149-158页

作者： Zhou, Quan Cheung, C. S. Leung, C. W. Li, Xiaotian Li, Xiaojie Huang, Zuohua Hong Kong Polytech Univ Dept Mech Engn Kowloon Hong Kong Peoples R China Xi An Jiao Tong Univ State Key Lab Multiphase Flow Power Engn Xian 710049 Shaanxi Peoples R China

Biomass-derived syngas composition varies considerably depending on different feedstocks and processing techniques and thereby complicates the combustion control. A study on the effects of variations in the fuel composition and initial pressure on the characteristics of premixed H-2/CO/CH4 flames was conducted using the spherical expanding flame method and CHEMIKIN package. Experimental measurements and numerical simulations were performed at an initial temperature of 303 K, equivalence ratios of 0.6-1.5 and pressures of 0.1-0.5 MPa with a wide range of H-2/CO/CH4 compositions. The thermal and chemical kinetic analyses are also presented. The measured laminar flame speed was compared with simulations using the Li mechanism. The experimental data show a reasonable agreement with the calculated values, especially at fuel-lean and low pressure conditions. With the increase of H-2 fraction in the fuel, the laminar flame speed increases significantly, but for the CH4 enrichment flame, the behavior is quite the contrary that it has the lowest laminar flame speed. With the increase of CO fraction in the fuel, the laminar flame speed does not change much. The thermal and chemical kinetic analyses indicate that the CO addition has more effect on the adiabatic flame temperature but only plays a small role in the chemical effect compared to that of the H-2 addition. On the other hand, at elevated pressures, the Li mechanism gives slight overestimations for lean mixtures but underestimations for rich mixtures. The laminar flame speed decreases with the increase of initial pressure under tested equivalence ratio which is mainly due to the increasing unburned mixture density and decreasing H, OH radicals concentrations.

关键词： Bio-syngas Laminar flame speed Elevated pressure Chemical kinetic structure

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