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Early and Sensitive Detection of Pathogens for Public Health and Biosafety:An Example of Surveillance and Genotyping of SARS-CoV-2 in Sewage Water by Cas12a-Facilitated Portable Plasmonic Biosensor

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Research 2024年第2期2023卷 259-272页

作者： Tianzhong Li Yuzhi Chen Zhi Chen Yuan Hao Minyi Liang Yingxia Liu Guanyong Ou Huanian Zhang Yuxuan Tang Yabing Hao Swelm Wageh Omar AAl-Hartomy Abul Kalam Bin Zhang Xin Shi Xuejin Li Han Zhang College of Physics and Optoelectronic Engineering Institute of Translational MedicineDepartment of OtolaryngologyShenzhen Second People's HospitalFirst Affiliated Hospital of Shenzhen UniversityHealth Science CenterShenzhen UniversityShenzhen 518060China Shenzhen Key Laboratory of Sensor Technology Shenzhen 518060China The Sixth Affiliated Hospital of Guangzhou Medical University Qingyuan People's HospitalQingyuan 511518China Shenzhen Key Laboratory of Pathogen and Immunity National Clinical Research Center for Infectious DiseaseState Key Discipline of Infectious DiseaseShenzhen Third People's HospitalSecond Hospital Affiliated to Southern University of Science and TechnologyShenzhen 518112China School of Medicine Southern University of Science and TechnologyShenzhen 518055China School of Physics and Optoelectronic Engineering Shandong University of TechnologyZibo 255049China Department of Physics Faculty of ScienceKing Abdulaziz University Jeddah 21589Saudi Arabia Research Center for Advanced Materials Science(RCAMS) King Khalid UniversityP.0.Box 9004Abha 61413Saudi Arabia Department of Chemistry College of ScienceKing Khalid UniversityP.0.Box9004Abha 61413 Saudi Arabia Health Sciences Institute China Medical UniversityShenyang 110000China The Chinese University of Hong Kong Shenzhen 518060China

Infectious diseases severely threaten public health and global *** addition to transmission through the air,pathogenic microorganisms have also been detected in environmental liquid samples,such as sewage *** biochemical detection methodologies are time-consuming and cost-ineffective,and their detection limits hinder early *** the present study,ultrafine plasmonic fiber probes with a diameter of 125μm are fabricated for clustered regularly interspaced short palindromic repeats/CRISPR-associated protein(CRISPR/Cas)-12a-mediated sensing of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).Single-stranded DNA exposed on the fiber surface is trans-cleaved by the Cas12a enzyme to release gold nanoparticles that are immobilized onto the fiber surface,causing a sharp reduction in the surface plasmon resonance(SPR)*** proposed fiber probe is virus-specific with the limit of detection of~2,300 copies/ml,and genomic copy numbers can be reflected as shifts in wavelengths.A total of 21 sewage water samples have been examined,and the data obtained are consistent with those of quantitative polymerase chain reaction(qPCR).In addition,the Omicron variant and its mutation sites have been fast detected using S gene-specific *** study provides an accurate and convenient approach for the real-time surveillance of microbial contamination in sewage water.

关键词： fiber consuming hinder

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Mn4+activated phosphors in photoelectric and energy conversion devices

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能源化学 2023年第11期86卷 277-299页

作者： Yang Ding Chunhua Wang Lang Pei Qinan Mao Sateesh Bandaru Runtian Zheng Soumyajit Maitra Meijiao Liu Li-Hua Chen Bao-Lian Su Jiasong Zhong College of Materials and Environmental Engineering Hangzhou Dianzi UniversityHangzhou 310018ZhejiangChina School of Energy and Environment City University of Hong KongKowloon 99077Hong KongChina Laboratory of Inorganic Materials Chemistry(CMI) University of Namur61 rue de Bruxelles B-5000NamurBelgium Department of Materials University of OxfordOxford OX1 3PHUK School of Chemistry and Chemical Engineering Zhejiang Sci-Tech UniversityHangzhou 310018ZhejiangChina State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of TechnologyWuhan 430070HubeiChina Laboratory of Inorganic Materials Chemistry(CMI) University of Namur61 rue de Bruxelles B-5000NamurBelgium State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of TechnologyWuhan 430070HubeiChina

Owing to their high luminous efficiency and tunable emission in both red light and far-red light regions,Mn4+ ion-activated phosphors have appealed significant interest in photoelectric and energy conversion devices such as white light emitting diode(W-LED),plant cultivation LED,and temperature *** to now,Mn4*has been widely introduced into the lattices of various inorganic hosts for brightly red-emitting ***,how to correlate the structure-activity relationship between host frame-work,luminescence property,and photoelectric device is urgently *** this review,we thor-oughly summarize the recent advances of Mn4+doped ***,several strategies like co-doping and defect passivation for improving Mn4+ emission are also *** importantly,the relationship between the protocols for tailoring the structures of Mn4+doped phosphors,increased luminescence performance,and the targeted devices with efficient photoelectric and energy conversion efficiency is deeply ***,the challenges and perspectives of Mn4+doped phosphors for prac-tical applications are *** cordially anticipate that this review can deliver a deep comprehen-sion of not only Mn4+luminescence mechanism but also the crystal structure tailoring strategy of phosphors,so as to spur innovative thoughts in designing advanced phosphors and deepening the applications.

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In Situ Induced Asymmetric Schottky Contacts Via Localized Ultrafast Laser Irradiation for Ultrasensitive, Self-Powered, 2d Photodetectors

SSRN

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SSRN 2023年

作者： Peng, Jin Zou, Guisheng Huo, Jinpeng Xiao, Yu Sun, Tianming Li, Zehua Feng, Bin Liu, Lei Department of Mechanical Engineering State Key Laboratory of Tribology Key Laboratory for Advanced Manufacturing by Materials Processing Technology Ministry of Education of PR China Tsinghua University Beijing100084 China Taiyuan University of Technology Taiyuan030024 China

Self-powered photodetectors hold great promise for addressing power-related challenges in the "Internet of Things (IoT)"era. However, the self-powered performances of a metal-semiconductor-metal (MSM) structure photodetector are inherently limited due to the presence of the symmetric Schottky contacts, leading to canceling photocurrents in opposite directions, while constructing an asymmetric MSM structure demands complicated design or sustained external fields. Herein, we propose a novel in situ contact engineering strategy based on localized femtosecond (fs) laser irradiation, achieving an asymmetric band structure in an MSM-structure photodetector with pristine symmetric Schottky contacts. As a result, a MoS2 photodetector that exhibits exceptional photoresponse and self-powered performances is successfully fabricated. The device type transitions from a photoconductor to a photodetector, exhibiting a significant improvement in the rectification ratio from 1 to >104. A comprehensive study on fs laser-based contact engineering is conducted by investigating atom diffusion, element variation, and Schottky barrier height variation. Notably, the photodetector demonstrates an exceptional zero-bias responsivity of 1.72A/W, with a short circuit current of 37.2nA and maximum output electric power of 1.67nW. Our work provides a new strategy for modifying the energy band structure and facilitates the fabrication of high-performance self-powered photodetectors. © 2023, The Authors. All rights reserved.

关键词： Femtosecond lasers

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Application of single-cell Raman-deuterium isotope probing to reveal the resistance of marine ammonia-oxidizing archaea SCM1 against common antibiotics

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Chemosphere 2024年 362卷 142500页

作者： Kong, Lingchao Wang, Yi Cui, Dongyu He, Wei Zhang, Chuanlun Zheng, Chunmiao State Environmental Protection Key Laboratory of Integrated Surface Water-Groundwater Pollution Control Guangdong Provincial Key Laboratory of Soil and Groundwater Pollution Control Southern University of Science and Technology Guangdong Shenzhen518055 China Shenzhen Key Laboratory of Marine Archaea Geo-Omics Department of Ocean Science and Engineering Southern University of Science and Technology Shenzhen518055 China Eastern Institute for Advanced Study Eastern Institute of Technology Ningbo315200 China

Antimicrobial resistance (AMR) in oceans poses a significant threat to human health through the seafood supply chain. Ammonia-oxidizing archaea (AOA) are important marine microorganisms and play a key role in the biogeochemical nitrogen cycle around the world. However, the AMR of marine AOA to aquicultural antibiotics is poorly explored. Here, Raman-deuterium isotope probing (Raman-DIP), a single-cell tool, was developed to reveal the AMR of a typical marine species of AOA, Nitrosopumilus maritimus (designated SCM1), against six antibiotics, including erythromycin, tetracycline, novobiocin, neomycin, bacitracin, and vancomycin. The D2O concentration (30% v/v) and culture period (9 days) were optimized for the precise detection of metabolic activity in SCM1 cells through Raman-DIP. The relative metabolic activity of SCM1 upon exposure to antibiotics was semi-quantitatively calculated based on single-cell Raman spectra. SCM1 exhibited high resistance to erythromycin, tetracycline, novobiocin, neomycin, and vancomycin, with minimum inhibitory concentration (MIC) values between 100 and 400 mg/L, while SCM1 is very sensitive to bacitracin (MIC: 0.8 mg/L). Notably, SCM1 cells were completely inactive under the metabolic activity minimum inhibitory concentration conditions (MA-MIC: 1.6–800 mg/L) for the six antibiotics. Further genomic analysis revealed the antibiotic resistance genes (ARGs) of SCM1, including 14 types categorized into 33 subtypes. This work increases our knowledge of the AMR of marine AOA by linking the resistant phenome to the genome, contributing to the risk assessment of AMR in the underexplored ocean environment. As antibiotic resistance in marine microorganisms is significantly affected by the concentration of antibiotics in coastal environments, we encourage more studies concentrating on both the phenotypic and genotypic antibiotic resistance of marine archaea. This may facilitate a comprehensive evaluation of the capacity of marine microorganisms t

关键词： Antibiotics

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Continuous synthesis of extremely small-sized iron oxide nanoparticles used for T_(1)-weighted magnetic resonance imaging via a fluidic reactor

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Science China Materials 2022年第6期65卷 1646-1654页

作者： Yu Mao Yan Li Fengchao Zang Haoli Yu Sen Yan Qingsong Song Zhiguo Qin Jianfei Sun Bo Chen Xiao Huang Ning Gu State Key Laboratory of Bioelectronics Jiangsu Key Laboratory for Biomaterials and DevicesSchool of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Jiangsu Key Laboratory of Molecular and Functional Imaging Medical SchoolSoutheast UniversityNanjing 210096China State Key Laboratory of Flexible Electronics&Institute of Advanced Materials Jiangsu National Synergistic Innovation Center for Advanced MaterialsNanjing Tech UniversityNanjing 210096China Materials Science and Devices Institute Suzhou University of Science and TechnologySuzhou 215009China

Extremely small-sized iron oxide nanoparticles(ESIONPs)with sizes less than 5 nm have shown great promise as T_(1)contrast agents for magnetic resonance imaging(MRI).However,their facile and scalable production with simultaneously endowed biocompatible surface chemistry remains difficult to be *** this study,by using the coprecipitation method implemented in a specially designed gas/liquid mixed phase fluidic reactor,polyglucose sorbitol carboxymethyether(PSC)coated ESIONPs were continuously synthesized with controllable particle sizes ranging from 1.8 to 4 *** the differently sized ESIONPs,the 3.7-nm ESIONPs exhibit the best performance as T_(1)MRI contrast agent,featuring a high r_(1) value of 4.11(mmol L^(−1))^(−1)s^(−1)and low r_(2)/r_(1) ratio of 7.90 under a clinical 3 T MR scanning,as well as the excellent T_(1)MRI contrast effect in not only water but also the cellular environment and blood ***,the ESIONPs possess long-term stability and good dispersity in aqueous dispersions,making them ideal candidates as safe and effective T_(1)-weighted MRI contrast agent for real clinical use.

关键词： ESIONPs T1-contrast agents MRI coprecipitation method gas/liquid mixed phase fluidic reactor

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Research of the flow boiling heat transfer on surfaces of stainless steel and anodized-chromium in mini-channel

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Annals of Nuclear Energy 2025年 222卷

作者： Zhang, Luteng Tang, Jiaxuan Lian, Qiang Xu, Wenbin Lu, Qi Tang, Simiao Zhu, Longxiang Ma, Zaiyong Sun, Wan Pan, Liangming Yan, Meiyue Key Laboratory of Low-grade Energy Utilization Technologies and Systems Ministry of Education Chongqing University Chongqing400044 China Department of Nuclear Engineering and Technology Chongqing University Chongqing400044 China State Key Laboratory of Advanced Nuclear Energy Technology Chengdu610041 China Department of Mechanical Engineering College of Design and Engineering National University of Singapore 9 Engineering Drive 1 117575 Singapore

The application of mini-channels provides many practical benefits in several industries. Surface modification by chromium coating has the advantage of easy production and corrosion resistance to enhance heat transfer. Based on the method of electroplating and anodizing, the chromium coating layer on stainless steel surface is prepared with lower contact angle and higher number of activated vaporization cores. A comparative experimental study is conducted in mini-channels for stainless steel surfaces and anodized-chromium surfaces to investigate the subcooled flow boiling behavior. Based on the visual image and measurement analysis, the bubble behavior and heat transfer characteristics with increasing heat flux are investigated. The onset of initial boiling and reverse flow from anodized-chromium surface are earlier than that from stainless steel surface. The average and the maximum heat transfer coefficient are increased by 11.8% and 14.1% on anodized-chromium surface respectively. © 2025 Elsevier Ltd

关键词： Electric heating

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Phase thermal stability and mechanical properties analyses of(Cr,Fe,V)–(Ta,W)multiple-based elemental system using a compositional gradient film

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International Journal of Minerals,Metallurgy and Materials 2020年第10期27卷 1379-1387页

作者： Qiu-wei Xing Jiang Ma Yong Zhang Beijing Advanced Innovation Center of Materials Genome Engineering State Key Laboratory of Advanced Metals and MaterialsUniversity of Science and Technology BeijingBeijing 100083China Guangdong Provincial Key Laboratory of Micro/Nano Optomechatronics Engineering College of Mechatronics and Control EngineeringShenzhen UniversityShenzhen 518060China

High-entropy alloys(HEAs)generally possess complex component combinations and abnormal *** traditional methods of investigating these alloys are becoming increasingly inefficient because of the unpredictable phase transformation and the combination of many *** development of compositionally complex materials such as HEAs requires high-throughput experimental methods,which involves preparing many samples in a short *** we apply the high-throughput method to investigate the phase evolution and mechanical properties of novel HEA film with the compositional gradient of(Cr,Fe,V)-(Ta,W).First,we deposited the compositional gradient film by ***,the mechanical properties and thermal stability of the(Cr0.33Fe0.33V0.33)x(Ta0.5W0.5)100−x(x=13-82)multiplebased-elemental(MBE)alloys were *** the deposited wafer was annealed at 600℃for 0.5 h,the initial amorphous phase was transformed into a body-centered cubic(bcc)structure phase when x=*** were observed on the film surface when x was 72 and ***,the highest hardness of as-deposited films was found when x=18,and the maximum hardness of annealed films was found when x=33.

关键词： high-throughput fabrication hard coating thermal resistance mechanical property phase stability high-entropy alloys

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Application of Fuzzy Interpolation Reasoning in Welding Process Decision 32nd

Application of Fuzzy Interpolation Reasoning in Welding Proc...

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32nd International Ocean and Polar engineering Conference, ISOPE 2022

作者： Pan, Zufu Feng, Zhiqiang Qin, Haijian Jiao, Ziquan Yuan, Hao Li, Xin School of Mechamical Engineering Guangxi University Guangxi Nanning China Guangxi Engineering Technology Research Center of Ship Digital Design and Advanced Manufacturing Beibu Gulf University Guangxi Qinzhou China State Key Laboratory of Hydraulic Engineering Simulation & Safety Tianjin University Tianjin China

ISBN: (纸本)9781880653814

Welding is a highly nonlinear, time-varying and multi coupling typical complex process. When using the traditional fuzzy reasoning method to make decision on welding parameters, it is difficult to build an accurate and complete welding process parameter rule base due to the uncertainty and complexity of the welding process. When the given input value cannot match the rules in the rule base, the reasoning conclusion cannot be drawn. In order to solve this limitation, this paper proposes a welding process parameter decision-making method, which is achieved by fuzzy rule interpolation. It can predict and decide welding parameters when the welding process parameter rule base is sparse. The experimental results show that the model can achieve high accuracy. © 2022 by the International Society of Offshore and Polar Engineers (ISOPE).

关键词： Decision making

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Evolution of electromechanical properties in Fe-doped(Pb,Sr)(Zr,Ti)O_(3) piezoceramics

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Journal of advanced Ceramics 2021年第3期10卷 587-595页

作者： Chuan CHEN Yan WANG Zong-Yue LI Chun LIU Wen GONG Qing TAN Bing HAN Fang-Zhou YAO Ke WANG Joint Laboratory of Electric Power Intelligent Sensing Technology and Application Department of Sensing Technology for Electric PowerGlobal Energy Interconnection Research Institute Co.Ltd.Future Science ParkBeijing 102209China China Aerospace Science and Industry Corporation Beijing 100048China Naqu Branch of Tibet Electric Limited Company Naqu 852000China State Key Laboratory of New Ceramics and Fine Processing School of Materials Science and EngineeringTsinghua UniversityBeijing 100084China Center of Advanced Ceramic Materials and Devices Yangtze Delta Region Institute of Tsinghua UniversityZhejiang 314006China Beijing Advanced Innovation Center for Materials Genome Engineering State Key Laboratory for Advanced Metals and MaterialsUniversity of Science and Technology BeijingBeijing 100083China Department of Orthodontics&National Engineering Laboratory for Digital and Material Technology Peking University School and Hospital of StomatologyBeijing 100081China State Key Laboratory for Mechanical Behavior of Materials Xi'an Jiaotong UniversityXi'an 710049China Foshan(Southern China)Institute for New Materials Foshan 528200China

Defects in acceptor-doped perovskite piezoelectric materials have a significant impact on their electrical ***,the defect mediated evolution of piezoelectric and ferroelectric properties of Fe-doped(Pb,Sr)(Zr,Ti)O_(3)(PSZT-Fe)piezoceramics with different treatments,including quenching,aging,de-aging,and poling,was investigated *** vacancies with a cubic symmetry are preserved in the quenched PSZT-Fe ceramics,rendering them robust ferroelectric *** the aged PSZT-Fe polycrystals,defect dipole between Fe dopant and oxygen vacancy has the same orientation with spontaneous polarization Ps,which enables the reversible domain switching and hence leads to the emergence of pinched polarization hysteresis and recoverable strain *** the defect dipoles can be gradually disrupted by bipolar electric field cycling,once again endowing the aged materials with representative ferroelectric *** the poled PSZT-Fe polycrystals,the defect dipoles are reoriented to be parallel to the applied poling field,and an internal bias field aligning along the same direction emerges simultaneously,being responsible for asymmetric hysteresis loops.

关键词： lead-zirconate-titanate(PZT) piezoelectric acceptor defect engineering electromechanical properties

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Oxidation Behavior and Corrosion Mechanism of Sic Ceramics at High Temperatures in Wet Air Environment

SSRN

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SSRN 2023年

作者： Yan, Shuaibin Duan, Xiaoming Cai, Delong He, Peigang Yang, Zhihua Jia, Dechang Lin, Hua-Tay Zhou, Yu Institute for Advanced Ceramics School of Materials Science and Engineering Harbin Institute of Technology Harbin China Key Laboratory of Advanced Structural-Functional Integration Materials & Green Manufacturing Technology Harbin Institute of Technology Harbin China State Key Laboratory of Advanced Welding and Joining Harbin Institute of Technology Harbin150001 China Key Laboratory of Superlight Materials and Surface Technology Ministry of Education College of Materials Science and Chemical Engineering Harbin Engineering University 145 Nantong Street Harbin150001 China School of Electromechanical Engineering Guangdong University of Technology Guangzhou510006 China

The oxidation behavior and corrosion mechanism of SiC ceramics were investigated at high temperatures between 1000 and 1200 ℃ in wet environments (75% water/25% air). Experimental results showed that protective SiO2 layers formed at 1000 and 1100 ℃. Whereas, the oxidation and water vapor corrosion proceed simultaneously at 1200 ℃, resulting in a significant weight loss. Consequently, a porous layer of 30 μm formed after 10 hours oxidation. The calculated activation energy based on the oxide layer thickness data versus time curves was 109 kJ/mol, which was lower than the values reported in dry air environments. © 2023, The Authors. All rights reserved.

关键词： Oxidation

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