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Beijing Keji Daxue Xuebao/Journal of University of Science and Technology Beijing 2007年第SUPPL. 2期29卷 162-165页

作者： Feng, Yali Li, Haoran Zhu, Xueyuan School of Civil and Environmental Engineering University of Science and Technology Beijing Beijing 100083 China State Key Laboratory of Biochemical Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing 100080 China

Some factors affecting single chamber direct microbial fuel cell performance such as iron concentration in the cathode electrode, substrates in anode chamber, substrate concentration and anode electrode area were studied with a single chamber direct microbial fuel cell. It was proved that the load voltage increased with the Fe concentration increasing under the same conditions. Electricity was enhanced as more electrons and protons were released during anode chamber reaction for the different substrates. The load voltage was also grown with the increase of the substrate concentration, but the saturation value of the substrate concentration was 0.72 g·L-1. The more Rhodoferax ferrireducens absorbed on the surface anode electrodes to transport the more electrons, and the load voltage was not multiple of the number of anode electrode. This study provided scientific base for the application of the single chamber direct microbial fuel cell.

关键词： Microbial fuel cells

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ASYMMETRIC LEAVES1/2 and JAGGED are required for sepal and petal organogenesis by defining organs from their boundaries

ASYMMETRIC LEAVES1/2 and JAGGED are required for sepal and p...

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18th International Conference on Arabidopsis Research(第十八届国际拟南芥大会)

作者： Ben Xu Ziyu Li Yan Zhu Hua Wang Hong Ma Aiwu Dong Hai Huang National Laboratory of Plant Molecular Genetics Shanghai Institute of Plant Physiology and EcologyShanghai Institutes for Biological SciencesChinese Academy of Sciences300 Fanglin RoadShanghai 200032China State Key Laboratory of Genetic EngineeringDepartment of BiochemistrySchool of Life SciencesFudan UniversityShanghai 200433China Department of Biology and the Huck Institute for the Life SciencesThe Pennsylvania State UniversityUniversity ParkPA 16802USA

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Effect of bovine serum albumin on the micellization of poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) block copolymers in aqueous solutions by fluorescence spectroscopy

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Science China Chemistry 2006年第6期49卷 541-549页

作者： GUO Chen WANG Jing LIANG Xiangfeng ZHENG Lili LIU Huizhou Laboratory of Separation Science and Engineering State Key Laboratory of Biochemical EngineeringInstitute of Process EngineeringGraduate University of Chinese Academy of SciencesBeijing 100080China

Effect of bovine serum albumin(BSA)on the temperature-dependent association behavior of poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide)(PEO-PPO-PEO)block copolymers was investigated using pyrene fluorescence *** critical micellization temperature(CMT)of pluronics in aqueous solution was increased by the addition of BSA.A closed association model was used to obtain the standard free energies(△DG^(0)),enthalpies(△DH^(0)),and entropies(△DS^(0))of *** standard enthalpy and entropy of micellization for pluronic polymers in water were decreased with an increase of the BSA *** more PPO component in the pluronic polymer,the higher the changed values of micellization enthalpy and *** hydrophobic part of the pluronics,PPO,was responsible for the interaction between pluronics and *** interaction between PPO and BSA was correlated to the alternation of the PPO-PPO interaction by the addition of BSA,which would shift the CMT toward higher temperature and alter the thermodynamic parameters of micellization for pluronics in aqueous solutions.

关键词： block copolymer bovine serum albumin micelle fluorescence spectroscopy.

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Enzymatic hydrolysis of cellulose materials treated with ionic liquid [BMIM] Cl

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Chinese Science Bulletin 2006年第20期51卷 2432-2436页

作者： LIU Liying CHEN Hongzhang State Key Laboratory of Biochemical Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing 100080 China

A new cellulose solvent ionic liquid 1-butyl-3-methylimidazolium chloride ([BMIM]Cl) was used to treat wheat straw and steam-exploded wheat straw (SEWS) in order to improve the enzymatic hy- drolysis rates, while the water was used as the con- trol. The enzymatic hydrolysis results showed that the hydrolysis rates of materials treated with [BMIM]Cl were improved. The hydrolysis rate of treated wheat straw could reach 70.37% and the SEWS could be completely hydrolyzed, while hydrolysis rates of the wheat straw and SEWS treated with water were 42.78% and 68.78% under the same conditions, re- spectively. The FTIR analysis and polymerization degree measurement indicated that the hydrolysis rates improvement was attributed to the decrease of the polymerization degrees of cellulose and hemi- cellulose, the absolute crystallinity degree of cellu- lose and the increase of its reaction accessibility.

关键词：纤维素材料离子性液体丁基氯化物酶水解 FTIR 生化工程

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Inside Front Cover: One‐Pot Synthesis and Hierarchical Assembly of Hollow Cu2O Microspheres with Nanocrystals‐Composed Porous Multishell and Their Gas‐Sensing Properties (Adv. Funct. Mater. 15/2007)

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Advanced Functional Materials 2007年第15期17卷

作者： H. Zhang Q. Zhu Y. Zhang Y. Wang L. Zhao B. Yu Multiphase Reaction Laboratory Institute of Process Engineering Chinese Academy of Sciences P.O. Box 353 Beijing 100080 (P.R. China) State Key Laboratory of Transducer Technology Institute of Electronics Chinese Academy of Sciences Beijing 100080 (P.R. China)

On p. 2766, Qinshan Zhu and co‐workers report on multishell hollow Cu 2 O microspheres that are synthesized by a facile and one‐pot solvothermal route. A two‐step organization process, in which hollow microspheres of Cu 2 (OH) 3 NO 3 are formed first followed by reduction to Cu 2 O by glutamic acid, leads to the special multishell and hollow microstructures. Interestingly, a Cu 2 O gas sensor fabricated with the multishell microspheres shows a much higher sensitivity to ethanol than solid Cu 2 O microspheres. Hierarchical assembly of hollow microstructures is of great scientific and practical value and remains a great challenge. This paper presents a facile and one‐pot synthesis of Cu 2 O microspheres with multilayered and porous shells, which were organized by nanocrystals. The time‐dependent experiments revealed a two‐step organization process, in which hollow microspheres of Cu 2 (OH) 3 NO 3 were formed first due to the Ostwald ripening and then reduced by glutamic acid, the resultant Cu 2 O nanocrystals were deposited on the hollow intermediate microspheres and organized into finally multishell structures. The special microstructures actually recorded the evolution process of materials morphologies and microstructures in space and time scales, implying an intermediate‐templating route, which is important for understanding and fabricating complex architectures. The Cu 2 O microspheres obtained were used to fabricate a gas sensor, which showed much higher sensitivity than solid Cu 2 O microspheres.

关键词： Hollow materials Microspheres Nanocrystals Sensors, gas

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Application of Block Copolymer in Three-Liquid-Phase Extraction System

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Tsinghua Science and Technology 2006年第2期11卷 248-251页

作者：申淑锋常志东孙兴华胡欣刘会洲 Graduate School of the Chinese Academy of Sciences Beijing 100080 China Laboratory of Separation Science and Engineering State Key Laboratory of Biochemical Engineering Institute of Process Engineering Chinese Academy of Sciences P. O. Box 353 Beijing 100080 China

A novel three-liquid-phase extraction system （TES） composed of butyl acetate, block copolymer polyethylene oxide-polypropylene oxide-polyethylene oxide and ammonium sulphate aqueous solution [（NH4）2SO4] as top, middle, and bottom phase, respectively, has been developed. The copolymer recycling and partitioning behavior of penicillin V has been studied in this system. Results show that the copolymer could be purified and recycled and penicillin V of the filtrated ferment broth could be partitioned unevenly among the phases and purified in the top phase of this TES. About 90 wt.% of penicillin V could be distributed into the top phase around pH 2.5 and only less than 0.1 wt.% left in the bottom phase.

关键词： three-liquid-phase extraction system block copolymer phenoxymethyl penicillin recovery

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Immure response induced by oral DNA vaccination against FMDV delivered by attenuated Salm onella choleraesuis C500

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Frontiers in Biology 2006年第2期1卷 110-114页

作者： Liu Mingqiu Niu Xiaofeng Yan Jingran Yan Weiyao Zheng Zhaoxin State Key Laboratory of Genetic Engineering Institute of GeneticsSchool of Life ScienceFudan UniversityShanghai 200433China Institute of Biochemistry and Cell Biology SIBSChinese Academy of SciencesShanghai 200031China

A recombinant strain of Salmonella choleraesuis C500,containing a eukaryotic expression plasmid pBO1 with the immune-dominant epitope of foot-and-mouth disease virus,was *** immune response to this recombinant strain was evaluated by oral administration of the recombinant live bacteria pBO1/*** in *** showed that T cell response and specific antibody production were *** approach may present a general strategy for eliciting immune responses with DNA vaccine delivered by live bacterial *** stimulated indexes of T lymphoproliferation by specific antigens of FMDV in rabbits,can reach up to 11.0 and an antibody titer of 1/32 as detected in the erum with liquid block ELISA.

关键词： Salmonella choleraesuis foot-and-mouth disease virus recombinant live vaccine

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Cover Picture: Preparation and Application of Novel Microspheres Possessing Autofluorescent Properties (Adv. Funct. Mater. 16/2007)

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Advanced Functional Materials 2007年第16期17卷

作者： W. Wei L.‐Y. Wang L. Yuan Q. Wei X.‐D. Yang Z.‐G. Su G.‐H. Ma National Key Laboratory of Biochemical Engineering Institute of Process Engineering Chinese Academy of Sciences 100080 Beijing (P.R. China) Graduate University of the Chinese Academy of Sciences 100049 Beijing (P.R. China) Medical and Healthy Analytical Center Peking University Health Science Center 100083 Beijing (P.R. China) Department of Chemical Biology State Key Laboratories of Natural and Biomimetic Drugs School of Pharmaceutical Sciences Peking University Health Science Center 100083 Beijing (P.R. China)

On p. 3153, Guang‐Hui Ma and co‐workers report on the development of autofluorescent chitosan microspheres with tunable color based on different crosslinking reagents and further chemical modification. The fluorescent intensity can also be controlled by the particle size and crosslinking degree. The autofluorescent microspheres may be useful in fluorescence assays as bright, inexpensive, and stable probes for qualitative and quantitative studies of biological interactions and drug delivery. Fluorescent microspheres are widely used as biological tracers. In this study, uniformly sized chitosan microspheres crosslinked with glutaraldehyde (CG microspheres) and formaldehyde (CF microspheres) are successfully prepared by the Shirasu Porous Glass (SPG) membrane emulsification technique. Selectively reduced CG microspheres (SRCG microspheres) are obtained by NaBH 4 reduction. These chitosan microspheres are found to exhibit fluorescent properties without conjugation to any fluorescent agent. The fluorescence color varies with different crosslinkers and can be modulated by further chemical reduction, whereas the fluorescence intensity can be controlled by tuning the particle size and degree of crosslinking. The autofluorescence of the microspheres is applied to study the phagocytosis of HepG2 cells using the microspheres as novel tracers. Quantitative and qualitative evaluations show that these chitosan microspheres serve as bright, inert, durable, and extremely photostable tracers.

关键词： Crosslinking Fluorescence imaging Microspheres Polymeric materials

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Increased production of recombinant prourokinase with porous microcarrier cell culture by periodic pressure oscillation in a stirred tank reactor

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High Technology Letters 2006年第3期12卷 311-317页

作者：胡显文 Gao Lihua Li Zuohu Xiao Chengzu Xu Zhaoping Department of Cell Engineering Institute of Bioteehnology Academy of Military Medical Sciences Beijing 100071 P.R. China State Key Laboratory of Biochemical Engineering Institute oi Process Engineering Chinese Academy of Sciences Beijing 100080 P. R. China

An rCHO cell line expressing recombinant human prourokinase （pro-UK） at the level of 5μg/ 10^6cells/d was cultivated on Cytopore cellulose porous microcarriers in a 7.5L Biostat CT stirred tank reactor. A periodic pressure oscillation of 0.04 MPa and 0.04 Hz was adopted to introduce a physical stimulus on the rCHO cells and to improve mass transfer characteristic between cells and medium in the process of porous microcarrier CHO cell culture. Compared to constant pressure culture, the oscillation culture didn＇t influence specific cell growth rate significantly, but could enhance the pro-UK specific production by 10% - 40%, and reduce production of lactate by 10% - 30%. In the perfusion culture of recombinant CHO cell with serum-free medium for 67 days, cell density could reach 2.64×10^7/ml, the maximal prourokinase concentration in harvested supernatant was about 118mg/L, a total of 21.1 grams of prourokinase was produced in 313 liters of supernatant. In conclusion, the perfusion cell culture with periodic pressure oscillation can enhance the production of recombinant protein and increase the reactor specific productivity.

关键词： cell culture porous microcarrier prourokinase periodic pressure oscillation

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Phase behavior of ionic liquids (II) Aqueous two-phase

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Huagong Xuebao/Journal of Chemical Industry and engineering (China) 2006年第9期57卷 2149-2151页

作者： Xia, Hansong Yu, Jiang Hu, Xuesheng Liu, Qingfen Liu, Huizhou Laboratory of Separation Science and Engineering Institute of Process Engineering Chinese Acad. of Sci. Beijing 100080 China State Key Laboratory of Biochemical Engineering Institute of Process Engineering Chinese Acad. of Sci. Beijing 100080 China Graduate School Chinese Acad. of Sci. Beijing 100039 China

The phase behavior of ionic liquids aqueous two-phase (ILATPS) was investigated. It was found that the additive salt had little effect on the phase equilibrium of aqueous two-phase. The effect of anion mainly obeyed the Hofmeister rule, and the formation of ILATPS could be promoted by the increase of the hydrophobicity of anion. The formation of ILATPS could also be promoted by the increase of polarity of the head group, and by the increase of the side-chain length of ionic liquids, but the effect was weaker than that anion. An elevated temperature would inhibit the formation of ILATPS for short-chain ionic liquids, but would promote the formation of ILATPS for long-chain ionic liquids.

关键词： Ionization of liquids

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