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3D Printing of Tough Hydrogel Scaffolds with Functional Surface Structures for Tissue Regeneration

Nano-Micro Letters 2025年第2期17卷 18-45页

作者： Ke Yao Gaoying Hong Ximin Yuan Weicheng Kong Pengcheng Xia Yuanrong Li Yuewei Chen Nian Liu Jing He Jue Shi Zihe Hu Yanyan Zhou Zhijian Xie Yong He State Key Laboratory of Fluid Power and Mechatronic Systems School of Mechanical EngineeringZhejiang UniversityHangzhou 310027People’s Republic of China Liangzhu Laboratory Zhejiang UniversityHangzhou 310027People’s Republic of China Key Laboratory of 3D Printing Process and Equipment of Zhejiang Province College of Mechanical EngineeringZhejiang UniversityHangzhou 310027People’s Republic of China Stomatology Hospital School of StomatologyZhejiang University School of MedicineHangzhou 310027People’s Republic of China State Key Laboratory of Advanced Welding and Joining Harbin Institute of TechnologyHarbin 150001People’s Republic of China Institute of Digital Medicine Nanjing First HospitalNanjing Medical UniversityNanjing 210006People’s Republic of China

Hydrogel scaffolds have numerous potential applications in the tissue engineering ***,tough hydrogel scaffolds implanted in vivo are seldom reported because it is difficult to balance biocompatibility and high mechanical *** by Chinese ramen,we propose a universal fabricating method(printing-P,training-T,cross-linking-C,PTC&PCT)for tough hydrogel scaffolds to fill this ***,3D printing fabricates a hydrogel scaffold with desired structures(P).Then,the scaffold could have extraordinarily high mechanical properties and functional surface structure by cycle mechanical training with salting-out assistance(T).Finally,the training results are fixed by photo-cross-linking processing(C).The tough gelatin hydrogel scaffolds exhibit excellent tensile strength of 6.66 MPa(622-fold untreated)and have excellent ***,this scaffold possesses functional surface structures from nanometer to micron to millimeter,which can efficiently induce directional cell ***,this strategy can produce bionic human tissue with mechanical properties of 10 kPa-10 MPa by changing the type of salt,and many hydrogels,such as gelatin and silk,could be improved with PTC or PCT *** experiments show that this scaffold can effectively promote the new generation of muscle fibers,blood vessels,and nerves within 4 weeks,prompting the rapid regeneration of large-volume muscle loss injuries.

关键词： 3D printing Tough hydrogel scaffold Functional surface structure Tissue regeneration Biomaterials

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Green Synthesis of Multifunctional Cellulose-Based Eutectogel Using a Metal Salt Hydrate-Based Deep Eutectic Solvent for Sustainable Self-Powered Sensing

SSRN

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SSRN 2024年

作者： Zhu, Jiaxuan Shao, Changyou Hao, Sanwei Xue, Kai Zhang, Jifei Sun, Zhiwen Xiao, Ling-Ping Ren, Wenfeng Yang, Jun Cao, Bobo Sun, Runcang Liaoning Key Laboratory of Lignocellulose Chemistry and BioMaterials Liaoning Collaborative Innovation Center for Lignocellulosic Biorefinery College of Light Industry and Chemical Engineering Dalian Polytechnic University Dalian116034 China School of Materials Science and Engineering Shandong University of Technology Zibo255000 China Beijing Key Laboratory of Ionic Liquids Clean Process State Key Laboratory of Mesoscience and Engineering CAS Key Laboratory of Green Process and Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing100190 China Beijing Key Laboratory of Lignocellulosic Chemistry Beijing Forestry University Beijing100190 China Beijing Forestry University China Tsinghua University China

Renewable cellulose biopolymers offer a promising alternative to petroleum-derived synthetic polymers for preparing advanced eutectogel materials in sustainable electronics. However, it remains a great challenge for the efficient dissolution of cellulose and subsequent green synthesis of polymer gel networks in a green and sustainable manner. Herein, we propose a one-pot strategy in a green deep eutectic solvents (DES) system which simultaneously enable to efficiently dissolve cellulose and proceed sunlight-induced photopolymerization into eutectogel without the need for cross-linking agents or initiators. This DES system is comprised of zinc chloride (ZnCl2) hydrate as the hydrogen bond acceptor and acrylic acid (AA) as the donor, facilitating the dissolution of cellulose by breaking the hydrogen bonds within cellulose chains. Importantly, the subsequent in-situ photopolymerized cellulose eutectogel yeilds superior fracture strength (14 MPa), large fracture strain (980%), high toughness (65 MJ m-3), and self-healing properties. Additionally, the incorporation of an inorganic salt imparts the high conductivity and significant freeze resistance (-50 °C) to the eutectogel, which is applicable to be a soft electrode in triboelectric nanogenerator (TENG) for wireless motion detection and human–computer interaction control. This work sheds light on the rational design of advanced stretchable conductive biopolymer materials in sustainable and self-powered wearable electronics. © 2024, The Authors. All rights reserved.

关键词： Zinc chloride

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The PIWI-specific insertion module helps load longer piRNAs for translational activation essential for male fertility

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Science China(Life Sciences) 2023年第7期66卷 1459-1481页

作者： Xin Wang Di-Hang Lin Yue Yan An-Hui Wang Jiaoyang Liao Qian Meng Wen-Qing Yang Heng Zuo Min-Min Hua Fengjuan Zhang Hongwen Zhu Hu Zhou Tian-Yu Huang Rui He Guangyong Li Yue-Qiu Tan Hui-Juan Shi Lan-Tao Gou Dangsheng Li Ligang Wu Yonggang Zheng Xiang-Dong Fu Jinsong Li Rujuan Liu Guo-Hui Li Mo-Fang Liu Key Laboratory of Systems Health Science of Zhejiang Province School of Life ScienceHangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou310024China State Key Laboratory of Molecular Biology State Key Laboratory of Cell BiologyShanghai Key Laboratory of Molecular AndrologyShanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai200031China Laboratory of Molecular Modeling and Design State Key Laboratory of Molecular Reaction DynamicsDalian Institute of Chemical PhysicsChinese Academy of SciencesDalian116023China Department of Analytical Chemistry and CAS Key Laboratory of Receptor Research Shanghai Institute of Materia MedicaChinese Academy of SciencesShanghai201203China School of Life Science and Technology ShanghaiTech UniversityShanghai201210China NHC Key Lab of Reproduction Regulation(Shanghai Institute for Biomedical and Pharmaceutical Technologies) Fudan UniversityShanghai200032China Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education School of Basic Medical SciencesNingxia Medical UniversityYinchuan750004China Department of Urology General Hospital of Ningxia Medical UniversityNingxia Medical UniversityYinchuan750004China Institute of Reproductive and Stem Cell Engineering College of Basic of MedicineCentral South UniversityChangsha410000China Department of Physiology Howard Hughes Medical InstituteUniversity of Texas Southwestern Medical CenterDallasTX75390USA Westlake Laboratory of Life Sciences and Biomedicine School of Life SciencesWestlake UniversityHangzhou310024China

PIWI-clade proteins harness pi RNAs of 24–33 nt in *** great puzzles are how PIWI-clade proteins incorporate pi RNAs of different sizes and whether the size matters to PIWI/pi RNA *** we report that a PIWI-Ins module unique in PIWIclade proteins helps define the length of pi *** of PIWI-Ins in Miwi shifts MIWI to load with shorter pi RNAs and causes spermiogenic failure in mice,demonstrating the functional importance of this regulatory ***,we show that longer pi RNAs provide additional complementarity to target m RNAs,thereby enhancing the assembly of the MIWI/e IF3f/Hu R super-complex for translational ***,we identify a c.1108C>T(p.R370W)mutation of HIWI(human PIWIL1)in infertile men and demonstrate in Miwi knock-in mice that this genetic mutation impairs male fertility by altering the property of PIWI-Ins in selecting longer pi *** findings reveal a critical role of PIWI-Ins-ensured longer pi RNAs in fine-tuning MIWI/pi RNA targeting capacity,proven essential for spermatid development and male fertility.

关键词： PIWI piRNAs PIWI-Ins translational activation spermatid development male fertility

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Cytochrome P450 Enzyme Design by Constraining the Catalytic Pocket in a Diffusion Model

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Research 2025年第1期2024卷 618-630页

作者： Qian Wang Xiaonan Liu Hejian Zhang Huanyu Chu Chao Shi Lei Zhang Jie Bai Pi Liu Jing Li Xiaoxi Zhu Yuwan Liu Zhangxin Chen Rong Huang Hong Chang Tian Liu Zhenzhan Chang Jian Cheng Huifeng Jiang Key Laboratory of Engineering Biology for Low-Carbon Manufacturing Tianjin Institute of Industrial BiotechnologyChinese Academy of SciencesTianjin 300308China University of Chinese Academy of Sciences Beijing 100049China National Center of Technology Innovation for Synthetic Biology Tianjin 300308China College of Biotechnology Tianjin University of Science and TechnologyTianjin 300457China Department of Biochemistry and Biophysics School of Basic Medical SciencesPeking UniversityBeijing 100191China College of Life Science and Technology Wuhan Polytechnic UniversityWuhanHubei 430023China State Key Laboratory of Elemento-Organic Chemistry College of ChemistryNankai UniversityTianjin 300071China College of Life Science Nankai UniversityTianjin 300071China

Although cytochrome P450 enzymes are the most versatile biocatalysts in nature,there is insufficient comprehension of the molecular mechanism underlying their functional innovation ***,by combining ancestral sequence reconstruction,reverse mutation assay,and progressive forward accumulation,we identified 5 founder residues in the catalytic pocket of flavone 6-hydroxylase(F6H)and proposed a"3-point fixation"model to elucidate the functional innovation mechanisms of P450s in *** to this design principle of catalytic pocket,we further developed a de novo diffusion model(P450Diffusion)to generate artificial ***,among the 17 non-natural P450s we generated,10 designs exhibited significant F6H activity and 6 exhibited a 1.3-to 3.5-fold increase in catalytic capacity compared to the natural *** work not only explores the design principle of catalytic pockets of P450s,but also provides an insight into the artificial design of P450 enzymes with desired functions.

关键词： cytochrome P progressive forward accumulationwe cytochrome p enzymes molecular mechanism ancestral sequence reconstructionreverse mutation assayand de novo design catalytic pocket functional innovation

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Sprayed Pickering emulsion with high antibacterial activity for wound healing

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Progress in Natural Science:Materials International 2020年第5期30卷 669-676页

作者： Xieru Bao Jie Wu Guanghui Ma State Key Laboratory of Biochemical Engineering Institute of Process Engineering Chinese Academy of Sciences School of Chemical Engineering University of Chinese Academy of Sciences PLA Key Laboratory of Biopharmaceutical Production and Formulation Engineering Institute of Process Engineering Chinese Academy of Sciences Jiangsu National Synergetic Innovation Center for Advanced Materials

Effective treatment of wound injuries can prevent infections and promote healing. In this work, a sprayed Pickering emulsion composed of chitosan nanoparticles, tea tree oil and curcumin was formulated. The shear thinning behaviour allowed this Pickering emulsion to spray even with a high viscosity provided by the aqueous phase containing chitosan nanoparticles. Moreover, the Pickering emulsion could recover to the high viscosity after spraying onto the wounds, leading to the long residence time and intimate contact. In addition, the Pickering emulsion provided a carrier to encapsulate the tea tree oil, which caused sustained-release effects and further strengthened tea tree oil’s antibacterial activity towards the Gram-negative Escherichia coli(E. coli),Gram-positive Staphylococcus aureus(S. aureus) and Gram-positive methicillin-resistant Staphylococcus aureus(MRSA). Besides, this multi-phase system offered a platform to load with hydrophobic curcumin, leading to better synergistic healing effects. Meanwhile, the Pickering emulsion avoided the irritation and toxicity related to surfactants, which was far safer than the classical emulsion especially when used in the defective skin. All these excellent properties endowed this sprayed Pickering emulsion with promising prospects for wound healing.

关键词： Pickering emulsion Wound healing Chitosan nanoparticles High viscosity Antibacterial activity

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Gram-Scale Synthesis of Unsupported Pt-Rare Earth Nanosponge for Enhanced Stability in Proton Exchange Membrane Fuel Cells

SSRN

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SSRN 2025年

作者： Li, Chenhao Wang, Hao Qian, Fangren Li, Siming Sang, Qinqin Chen, Qingjun Liao, Jialiang Zhu, Yihan Liu, Yanrong Zhang, Suojiang Li, Yawei Beijing Key Laboratory of Ionic Liquids Clean Process China School of Rare Earths University of Science and Technology of China Hefei230026 China Ganjiang Innovation Academy Chinese Academy of Sciences Ganzhou341119 China CAS Key Laboratory of Green Process and Engineering State Key Laboratory of Mesoscience and Engineering Beijing Key Laboratory of Solid State Battery and Energy Storage Process Institute of Process Engineering Chinese Academy of Sciences Beijing100190 China School of Chemical Engineering University of Chinese Academy of Sciences Beijing100049 China Longzihu New Energy Laboratory School of Energy Science and Technology Henan University Zhengzhou450000 China School of Chemistry and Chemical Engineering Shanxi University Taiyuan China Center for Electron Microscopy Institute for Frontier and Interdisciplinary Sciences State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology College of Chemical Engineering Zhejiang University of Technology Hangzhou China Shanxi University China

Durability is crucial for proton exchange membrane fuel cells (PEMFCs), but the current preferred catalyst, Pt/C, suffers from carbon corrosion and Pt detachment during cycling. In this work, we facilely synthesized carbon-free, unsupported PtLa catalysts on a gram-scale via arc melting coupled with a suction casting process, followed by a dealloying treatment. The resulting PtLa catalysts possess a nanosponge morphology, facilitating mass transport paths for the oxygen reduction reaction. In a PEM single cell, the nanosponge structure demonstrates high utilization of ionomers and proton conductivity with a minimal ionomer loading, and exhibits enhanced activity in the rated voltage region. A mass activity of 0.50 A mgPt-1 is achieved for the nanosponge PtLa-3 (NSPL-3), which is 2.9-fold greater than that of Pt/C (0.17 A mgPt-1), with 94.8% voltage retention and only 22.0% MA loss after 20,000 cyclic durability tests. The high activity retention of NSPL-3 is attributed to the high retention of its nanosponge-like structure, which maintains excellent connectivity between the active sites and leaves sufficient channels for mass transport. This approach holds the potential to propel fuel cell technology forward by providing a cost-effective and robust catalyst alternative. © 2025, The Authors. All rights reserved.

关键词： Dealloying

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Freestanding strontium vanadate/carbon nanotube films for long-life aqueous zinc-ion batteries

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Rare Metals 2022年第2期41卷 415-424页

作者： Ye-Hong Du Xian-Yu Liu Xin-Yu Wang Jun-Cai Sun Qiong-Qiong Lu Jian-Zhi Wang Ahmad Omar Daria Mikhailova Institute of Materials and Technology Dalian Maritime UniversityDalian 116026China e-mail:wangxinyu@*** School of Chemistry and Chemical Engineering Lanzhou City UniversityLanzhou 730070China Leibniz Institute for Solid State and Materials Research(IFW)Dresden E.V Helmholtzstraße 2001069 DresdenGermany e-mail:q.lu@ifw-dresden.de Key Laboratory for Green Chemical Process of Ministry of Education School of Chemical Engineering and PharmacyWuhan Institute of TechnologyWuhan 430205China

Aqueous rechargeable zinc-ion battery(ZIB)is considered to be a potential energy storage system for large-scale applications due to its environmental friendliness,high safety,and low ***,it remains challenging to develop suitable cathode materials with high specific capacity and long-term cyclic ***,we have fabricated freestanding Sr0.19V2O51.3H2O/carbon nanotubes(SrVO/CNTs)composite films with different mass ratios by incorporating SrVO into CNTs *** synthesized SrVO possesses a large interlayer spacing of 1.31 nm,which facilitates Zn(2+)***,the SrVO/CNTs composite film with conductive network structure promotes electron transfer and ensures good contact between SrVO and CNTs during the long-term cycling *** a result,the battery based on the SrVO/CNTs composite cathode with a mass ratio of 7:3 delivers a specific capacity of 326 mAh·g^(-1)at 0.1 A·g^(-1)and 145 mAh·g^(-1)at 5 A·g^(-1),demonstrating a high capacity and excellent rate ***,the assembled ZIB shows good capacity retention of 91%even after ultra-long cycling for 7500 cycles at a high current rate of 5 Ag^(-1).More importantly,the battery also delivers a high energy density and power density,as 290 Wh·kg^(-1)at 125 W·kg^(-1)(0.1 A·g^(-1)),or 115 Wh·kg^(-1)at 6078 W·kg^(-1)(5 Ag^(-1)).The results demonstrate that the SrVO/CNTs composite is a promising cathode toward large-scale energy storage applications.

关键词： Aqueous zinc-ion battery Strontium vanadate Carbon nanotubes network Freestanding composite film Long cycle life

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Acid-Base Bicentric Ionic Liquids Catalytic Reaction of Eg and Co2 into Carbonates Enhanced by Styrene Oxide

SSRN

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SSRN 2023年

作者： Meng, Chan Zhang, Heming Su, Qian Yan, Hanwen Du, Changyuan Li, Yunong Dong, Li Cheng, Weiguo Henan Institute of Advanced Technology Zhengzhou University Zhengzhou450001 China CAS Key Laboratory of Green Process and Engineering State Key Laboratory of Multiphase Complex Systems Beijing Key Laboratory of Ionic Liquids Clean Process Institute of Process Engineering Chinese Academy of Sciences Beijing100190 China Advanced Energy Science and Technology Guangdong Laboratory Guangdong Huizhou516003 China

In view of the harsh conditions of cyclic esterification reaction of ethylene glycol (EG) and carbon dioxide (CO2) to synthesize ethylene carbonate (EC), the yield is low and the catalysts are mostly metals, a series of green acid-base bicentric ionic liquids (ABbILs) were developed. Among them, the ABbILs of 1,5,7-Triazabicyclo[4.4.0]dec-5-ene glutarimide ([TBDH][Glu]) had the best catalytic performance. Under the optimal reaction conditions, the yield of EC reached 29.5%, which was higher than most of the conventional metal-based catalysts. By studying the ΔpKa value and thermogravimetry of ILs, it is found that the greater the ΔpKa of ILs, the stronger the stability of ILs. [TBDH] [Glu] has the highest thermal stability. Through activity screening results and DFT calculation, it was found that different cations affect the interaction between N on anion and H proton of EG, and the H on the cation can activate the O on EG, which would affect the ability of ABbILs to activate EG. The acid-base bicentered synergistic catalytic mechanism of Bronsted acid and Lewis base was proposed by characterization and DFT calculation. This study opens up new avenues for the efficient conversion of diols and CO2 for the preparation of cyclic carbonates. © 2023, The Authors. All rights reserved.

关键词： Ionic liquids

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Reinforcement Learning for Combinatorial Optimization of Train Timetable Rescheduling 9

Reinforcement Learning for Combinatorial Optimization of Tra...

引用

9th IEEE Smart World Congress, SWC 2023

作者： Shi, Qi Dai, Xuewu Cui, Dongliang Cheng, Lijuan Northeastern University The State Key Laboratory of Synthetical Automation for Process Industries Shenyang China Northumbria University Faculty of Engineering and Environment Newcastle Upon TyneNE1 8ST United Kingdom Purification Equipment Research Institute of Csic Handan China

ISBN: (纸本)9798350319804

With the advancement of computational intelligence, reinforcement learning has been proposed as a solution for train timetable rescheduling (TTR). However, traditional Q-learning approaches face challenges of large memory overhead and slow convergence rate due to the large state and action spaces in TTR. Moreover, Q-learning suffers from limited generalization ability, requiring retraining of the Q-table for handling new delay scenarios. To accelerate convergence speed and improve generalization ability, this paper proposes a deep reinforcement learning (DQN) approach for TTR. An adaptive action generation method is employed to address the slow convergence issue caused by a large action search space. In the proposed DQN, a target network and an experience replay mechanism are used to enhance the generalization ability of reinforcement learning. The performance of the proposed DQN is evaluated through simulation on a high-speed railway in China. Compared to the First-Come, First-Served (FCFS) baseline method, the validated algorithm yields an 80 % improvement in the generated schedules over FCFS. Moreover, the online decision-making process of the tested algorithm takes only 1-2 seconds, outperforming FCFS in terms of efficiency and effectiveness. © 2023 IEEE.

关键词： Deep Reinforcement Learning Q-learning Train Timetable Rescheduling

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Epigenetics in the modern era of crop improvements

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Science China Life Sciences 2025年第6期 1570-1609页

作者： Yan Xue Xiaofeng Cao Xiangsong Chen Xian Deng Xing Wang Deng Yong Ding Aiwu Dong Cheng-Guo Duan Xiaofeng Fang Lei Gong Zhizhong Gong Xiaofeng Gu Chongsheng He Hang He Shengbo He Xin-Jian He Yan He Yuehui He Guifang Jia Danhua Jiang Jianjun Jiang Jinsheng Lai Zhaobo Lang Chenlong Li Qing Li Xingwang Li Bao Liu Bing Liu Xiao Luo Yijun Qi Weiqiang Qian Guodong Ren Qingxin Song Xianwei Song Zhixi Tian Jia-Wei Wang Yuan Wang Liang Wu Zhe Wu Rui Xia Jun Xiao Lin Xu Zheng-Yi Xu Wenhao Yan Hongchun Yang Jixian Zhai Yijing Zhang Yusheng Zhao Xuehua Zhong Dao-Xiu Zhou Ming Zhou Yue Zhou Bo Zhu Jian-Kang Zhu Qikun Liu State Key Laboratory of Wheat Improvement Peking University Institute of Advanced Agricultural Sciences Shandong Laboratory of Advanced Agricultural Sciences in Weifang State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental Biology Chinese Academy of Sciences State Key Laboratory of Hybrid Rice College of Life Sciences Wuhan University School of Life Sciences Division of Life Sciences and Medicine University of Science and Technology of China State Key Laboratory of Genetic Engineering Collaborative Innovation Center of Genetics and Development Department of Biochemistry and Biophysics Institute of Plant Biology School of Life Sciences Fudan University Key Laboratory of Plant Design National Key Laboratory of Plant Molecular Genetics Shanghai Center for Plant Stress Biology CAS Center for Excellence in Molecular Plant Sciences Chinese Academy of Sciences Center for Plant Biology School of Life Sciences Tsinghua University Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE) Northeast Normal University State Key Laboratory of Plant Environmental Resilience Frontiers Science Center for Molecular Design Breeding College of Biological Sciences China Agricultural University College of Life Sciences Institute of Life Science and Green Development Hebei University Biotechnology Research Institute Chinese Academy of Agricultural Sciences College of Biology Hunan Key Laboratory of Plant Functional Genomics and Developmental Regulation Hunan Engineering and Technology Research Center of Hybrid Rapeseed Hunan University Institute of Advanced Agricultural Sciences School of Advanced Agricultural Sciences Peking University State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural University National Institute of Biological Sciences Key Laboratory of Seed Innovation Institute of Genetics and Developme

Epigenetic mechanisms are integral to plant growth, development, and adaptation to environmental stimuli. Over the past two decades, our comprehension of these complex regulatory processes has expanded remarkably, producing a substantial body of knowledge on both locus-specific mechanisms and genome-wide regulatory patterns. Studies initially grounded in the model plant Arabidopsis have been broadened to encompass a diverse array of crop species, revealing the multifaceted roles of epigenetics in physiological and agronomic traits. With recent technological advancements, epigenetic regulations at the single-cell level and at the large-scale population level are emerging as new focuses. This review offers an in-depth synthesis of the diverse epigenetic regulations, detailing the catalytic machinery and regulatory functions. It delves into the intricate interplay among various epigenetic elements and their collective influence on the modulation of crop traits. Furthermore, it examines recent breakthroughs in technologies for epigenetic modifications and their integration into strategies for crop improvement. The review underscores the transformative potential of epigenetic strategies in bolstering crop performance, advocating for the development of efficient tools to fully exploit the agricultural benefits of epigenetic insights.

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