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Primary cilia support cartilage regeneration after injury

International Journal of Oral Science 2023年第2期15卷 291-303页

作者： Dike Tao Lei Zhang Yunpeng Ding Na Tang Xiaoqiao Xu Gongchen Li Pingping Niu Rui Yue Xiaogang Wang Yidong Shen Yao Sun Department of Implantology School&Hospital of StomatologyTongji UniversityShanghaiChina Shanghai Engineering Research Center of Tooth Restoration and Regeneration ShanghaiChina State Key Laboratory of Cell Biology Shanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of Sciences University of Chinese Academy of Sciences ShanghaiChina Department of Oral and Maxillofacial Surgery School&Hospital of StomatologyTongji UniversityShanghaiChina Institute for Regenerative Medicine Shanghai East HospitalFrontier Science Center for Stem Cell ResearchShanghai Key Laboratory of Signaling and Disease ResearchSchool of Life Sciences and TechnologyTongji UniversityShanghaiChina Shanghai Institute of Stem Cell Research and Clinical Translation ShanghaiChin Key Laboratory of Big Data-Based Precision Medicine School of Engineering MedicineBeihang UniversityBeijingChina

In growing children,growth plate cartilage has limited self-repair ability upon fracture injury always leading to limb growth ***,one type of fracture injuries within the growth plate achieve amazing self-healing,however,the mechanism is *** this type of fracture mouse model,we discovered the activation of Hedgehog(Hh)signaling in the injured growth plate,which could activate chondrocytes in growth plate and promote cartilage repair.

关键词： fracture plate injuries

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Transductive Transfer Broad Learning Soft Sensor for Multimode Quality Prediction

Transductive Transfer Broad Learning Soft Sensor for Multimo...

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International Conference on Informative and Cybernetics for Computational Social Systems (ICCSS)

作者： Jialiang Zhu Hongying Deng Danya Xu Tao Yang Yi Liu Institute of Process Equipment and Control Engineering Zhejiang University of Technology Hangzhou China State Key Laboratory of Synthetical Automation for Process Industries Northeastern University Shenyang China

In chemical processes, reliable soft sensors are generally established by enough labeled data. However, in most multimode processes, the collection of sufficient labeled data is difficult due to the high cost and complexity. In this work, transductive transfer broad learning (TTBL) is proposed for multimode quality prediction. By transferring the useful information from the related domain, unlabeled data in the prediction domain is utilized for modeling. First, the data feature is extracted by the feature and enhancement nodes. The similarity information of current and related domain data is captured by the $k$ nearest-neighbor graph. Then, label information in the related domain can be transferred and similar information in the same domain can be retained by the manifold regularization framework. Finally, the output weight can be effectively calculated by the ridge regression algorithm. Experimental results on continuous stirred tank reactor datasets show the superiority of TTBL, compared with several common methods.

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Effect of the lead deposition on the performance of the negative electrode in an aqueous lead-carbon hybrid capacitor

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Journal of Energy Chemistry 2021年第4期30卷 509-516页

作者： Jinpeng Bao Nan Lin Haibo Lin Jiaxiang Guo Hongyu Gao Weiqi Gao State Key Laboratory of Inorganic Synthesis and Preparative Chemistry Jilin UniversityChangchun 130012JilinChina Institute of Energy and Process Systems Engineering Braunschweig University of TechnologyBraunschweigGermany Zhuhai College of Jilin University Zhuhai 519041GuangdongChina

Lead-carbon hybrid capacitors are the electrochemical devices between supercapacitors and lead-acid batteries,with low prices,stability in high and low temperature,good security and broad application *** paper introduces an electrodeposition behavior of Pb^(2+)on the negative electrode,which can improve the cycle life of the lead-carbon hybrid *** the charging process,lead ions in the electrolyte can diffuse from the positive electrode of the lead-carbon hybrid capacitor into the negative *** charging at a low current density,the lead ions around the negative electrode can be reduced to lead,and it is then quickly converted to lead sulfate *** the increase of the number of cycles,the final result is *** can reduce the specific surface area of the electric double layer,thereby reducing the capacitance performance of the carbon *** a result,it reduces the charge-discharge efficiency of the lead-carbon hybrid *** service life of lead-carbon hybrid capacitor is significantly improved by the inhibition of lead deposition by anion exchange *** capacity retention rate at 5 A/g is improved from 84%after 1000 cycles to 95%after 10,000 *** discovery of lead deposition in the negative electrode is conducive to improving the performance of long-life lead-carbon hybrid capacitors.

关键词： Lead-carbon hybrid capacitor Negative electrode Electrodeposition Cycle life

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Intensifying Electron Utilization by Surface-Anchored Rh Complex for Enhanced Nicotinamide Cofactor Regeneration and Photoenzymatic CO_(2)Reduction

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Research 2021年第1期2021卷 507-517页

作者： Yuqing Cheng Jiafu Shi Yizhou Wu Xueying Wang Yiying Sun Ziyi Cai Yu Chen Zhongyi Jiang Key Laboratory for Green Chemical Technology of Ministry of Education School of Chemical Engineering and TechnologyTianjin University92 Weijin RoadNankai DistrictTianjin 300072China Collaborative Innovation Center of Chemical Science and Engineering(Tianjin) 92 Weijin RoadNankai DistrictTianjin 300072China School of Environmental Science&Engineering Tianjin University92 Weijin RoadNankai DistrictTianjin 300072China State Key Laboratory of Biochemical Engineering Institute of Process EngineeringChinese Academy of SciencesBeijing 10090China

Solar-driven photocatalytic regeneration of cofactors,including reduced nicotinamide adenine dinucleotide(NADH),reduced nicotinamide adenine dinucleotide phosphate(NADPH),and reduced flavin adenine dinucleotide(FADH_(2)),could ensure the sustainable energy supply of enzymatic reactions catalyzed by oxidoreductases for the efficient synthesis of ***,the elevation of cofactor regeneration efficiency is severely hindered by the inefficient utilization of electrons transferred on the surface of *** by the phenomenon of ferredoxin-NADP+reductase(FNR)anchoring on thylakoid membrane,herein,a homogeneous catalyst of rhodium(Rh)complex,[Cp_(∗)Rh(bpy)H_(2)O]^(2+),was anchored on polymeric carbon nitride(PCN)mediated by a tannic acid/polyethyleneimine(TA/PEI)adhesive layer,acquiring PCN@TA/PEI-Rh core@shell *** by visible light,electrons were excited from the PCN core,then transferred through the TA/PEI shell,and finally captured by the surface-anchored Rh for instant utilization during the regeneration of *** TA/PEI-Rh shell could facilitate the electron transfer from the PCN core and,more importantly,achieved~1.3-fold elevation of electron utilization efficiency compared with ***,the PCN@TA/PEI-Rh afforded the NADH regeneration efficiency of 37.8%after 20 min reaction under LED light(405 nm)illumination,over 1.5 times higher than PCN with free *** of the NADH regeneration system with formate dehydrogenase achieved continuous production of formate from carbon dioxide(CO_(2)).Our study may provide a generic and effective strategy to elevate the catalytic efficiency of a photocatalyst through intensifying the electron utilization.

关键词： enzymatic dinucleotide catalyst

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Multi-scale feature fused stacked autoencoder and its application for soft sensor modeling

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Chinese Journal of Chemical engineering 2025年 81卷 241-254页

作者： Li, Zhi Xia, Yuchong Long, Jian Liu, Chensheng Zhang, Longfei State Key Laboratory of Industrial Control Technology East China University of Science and Technology Shanghai 200237 China Key Laboratory of Smart Manufacturing in Energy Chemical Process Ministry of Education East China University of Science and Technology Shanghai200237 China Huzhou Institute of Industrial Control Technology Huzhou313099 China Engineering Research Center of Process System Engineering Ministry of Education East China University of Science and Technology Shanghai200237 China

Deep Learning has been widely used to model soft sensors in modern industrial processes with nonlinear variables and uncertainty. Due to the outstanding ability for high-level feature extraction, stacked autoencoder (SAE) has been widely used to improve the model accuracy of soft sensors. However, with the increase of network layers, SAE may encounter serious information loss issues, which affect the modeling performance of soft sensors. Besides, there are typically very few labeled samples in the data set, which brings challenges to traditional neural networks to solve. In this paper, a multi-scale feature fused stacked autoencoder (MFF-SAE) is suggested for feature representation related to hierarchical output, where stacked autoencoder, mutual information (MI) and multi-scale feature fusion (MFF) strategies are integrated. Based on correlation analysis between output and input variables, critical hidden variables are extracted from the original variables in each autoencoder's input layer, which are correspondingly given varying weights. Besides, an integration strategy based on multi-scale feature fusion is adopted to mitigate the impact of information loss with the deepening of the network layers. Then, the MFF-SAE method is designed and stacked to form deep networks. Two practical industrial processes are utilized to evaluate the performance of MFF-SAE. Results from simulations indicate that in comparison to other cutting-edge techniques, the proposed method may considerably enhance the accuracy of soft sensor modeling, where the suggested method reduces the root mean square error (RMSE) by 71.8%, 17.1% and 64.7%, 15.1%, respectively. © 2025 Chemical Industry and engineering Society of China, and Chemical Industry Press Co., Ltd.

关键词： Frequency estimation

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Health risk ranking of antibiotic resistance genes in the Yangtze River

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Environmental Science and Ecotechnology 2024年第5期21卷 113-122页

作者： Chunxia Jiang Zelong Zhao Hans-Peter Grossart Feng Ju Yi Zhao Geoffrey Michael Gadd Ewa Korzeniewska Yuyi Yang Key Laboratory of Aquatic Botany and Watershed Ecology Wuhan Botanical GardenChinese Academy of SciencesWuhan430074China University of Chinese Academy of Sciences Beijing100049China Danjiangkou Wetland Ecosystem Field Scientific Observation and Research Station Chinese Academy of Sciences&Hubei ProvinceWuhan430074China Liaoning Key Laboratory of Marine Fishery Molecular Biology Liaoning Key Lab of Germplasm Improvement and Fine Seed Breeding of Marine Aquatic AnimalsLiaoning Ocean and Fisheries Science Research InstituteDalian116023China Leibniz-Institute for Freshwater Ecology and Inland Fisheries(IGB) Neuglobsow16775Germany Institute for Biochemistry and Biology Potsdam UniversityPotsdam14469Germany Key Laboratory of Coastal Environment and Resources of Zhejiang Province School of EngineeringWestlake UniversityHangzhou310030China School of Water Resources and Environment China University of Geosciences(Beijing)Beijing100083China Geomicrobiology Group School of Life SciencesUniversity of DundeeDundeeDD15EHScotlandUK State Key Laboratory of Heavy Oil Processing State Key Laboratory of Petroleum Pollution ControlChina University of PetroleumBeijing102249China Department of Water Protection Engineering and Environmental Microbiology Faculty of GeoengineeringUniversity of Warmia and Mazury in OlsztynOlsztyn10-720Poland

Antibiotic resistance is an escalating global health concern,exacerbated by the pervasive presence of antibiotic resistance genes(ARGs)in natural *** Yangtze River,the world's third-longest river,traversing areas with intense human activities,presents a unique ecosystem for studying the impact of these genes on human ***,we explored ARGs in the Yangtze River,examining 204 samples from six distinct habitats of approximately 6000 km of the river,including free-living and particle-associated settings,surface and bottom sediments,and surface and bottom bank *** shotgun sequencing,we generated an average of 13.69 Gb reads per *** findings revealed a significantly higher abundance and diversity of ARGs in water-borne bacteria compared to other habitats.A notable pattern of resistome coalescence was observed within similar habitat *** addition,we developed a framework for ranking the risk of ARG and a corresponding method for calculating the risk *** them,we identified water-borne bacteria as the highest contributors to health risks,and noted an increase in ARG risks in particle-associated bacteria correlating with heightened anthropogenic *** analysis using a weighted ARG risk index pinpointed the ChengdueChongqing and Yangtze River Delta urban agglomerations as regions of elevated health *** insights provide a critical new perspective on ARG health risk assessment,highlighting the urgent need for strategies to mitigate the impact of ARGs on human health and to preserve the ecological and economic sustainability of the Yangtze River for future human use.

关键词： Antibiotic resistance genes Yangtze River Habitat Coalescence Health risk

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Pickering interfacial biocatalysis with enhanced diffusion processes for CO_(2) mineralization

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Chinese Journal of Catalysis 2022年第4期43卷 1184-1191页

作者： Boyu Zhang Jiafu Shi Yang Zhao a Han Wang Ziyi Chu Yu Chen Zhenhua Wu Zhongyi Jiang School of Environmental Science&Engineering Tianjin UniversityTianjin 300072China Collaborative Innovation Center of Chemical Science and Engineering(Tianjin) Tianjin 300072China State Key Laboratory of Biochemical Engineering Institute of Process EngineeringChinese Academy of SciencesBeijing 100190China Key Laboratory for Green Chemical Technology of Ministry of Education School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China

Utilization of carbon dioxide(CO_(2))has become a crucial and anticipated solution to address environmental and ecological *** such as carbonic anhydrase(CA)can efficiently convert CO_(2) into various platform chemicals under ambient conditions,which offers a promising way for CO_(2) ***,we constructed a Pickering interfacial biocatalytic system(PIBS)stabilized by CA‐embedded MOFs(ZIF‐8 and ZIF‐L)for CO_(2) *** structure engineering of MOFs and incorporation of Pickering emulsion,the internal and external diffusion processes of CO_(2) during the enzymatic mineralization were greatly *** CO_(2) was ventilated at a flow rate of 50 mL min^(–1) for 1 h,the pH value of PIBS dropped from~8.00 to~6.50,while the average pH value of free system only dropped to~7.15,indicating that the initial reaction rate of CO_(2) mineralization of PIBS is nearly twice that of the free *** the 8^(th) cycle reaction,PIBS can still produce more than 9.8 mg of CaCO_(3) in 5 min,realizing efficient and continuous mineralization of CO_(2).

关键词： Carbon dioxide conversion Carbonic anhydrase Pickering emulsion Metal‐organic frameworks Diffusion intensification

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Stable confinement of Fe/Fe_(3)C in Fe,N-codoped carbon nanotube towards robust zinc-air batteries

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Chinese Chemical Letters 2021年第3期32卷 1121-1126页

作者： Lingbo Zong Xin Chen Shuming Dou Kaicai Fan Zumin Wang Wenjun Zhang Yunmei Du Jie Xu Xiaofei Jia Qi Zhang Xia Li Yida Deng Yanan Chen Lei Wang Taishan Scholar Advantage and Characteristic Discipline Team of Eco Chemical Process and Technology Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life ScienceMOEState Key Laboratory of Eco-chemical EngineeringCollege of Chemistry and Molecular EngineeringQingdao University of Science and TechnologyQingdao 266042China School of Materials Science and Engineering Key Laboratory of Advanced Ceramics and Machining Technology of Ministry of EducationTianjin Key Laboratory of Composite and Functional MaterialsTianjin UniversityTianjin 300072China Centre for Clean Environment and Energy School of Environment and ScienceGriffith UniversityQueensland 4222Australia Institute of Process Engineering Chinese Academy of SciencesBeijing 100190China

Catalytic oxygen reduction reaction(ORR)and oxygen evolution reaction(OER)have garnered great attention as the key character in metal-air ***,we developed a superior nonprecious bifunctional oxygen electrocatalyst,fabricated through spatial confinement of Fe/Fe_(3)C nanocrystals in pyridinic N and Fe-Nx rich carbon nanotubes(Fe/Fe_(3)C-N-CNTs).During ORR,the resultant electrocatalyst exhibits positive onset pote ntial of 1.0 V(***),large half-wave potentials of 0.88 V(***),which is more positive than Pt/C(0.98 V and 0.83 V,respectively).Remarkably,Fe/Fe_(3)C-N-CNTs exhibits outstanding durability and great methanol tolerance,exceeding Pt/C and most reported nonprecious metal-based oxygen reduction ***,Fe/Fe_(3)C-N-CNTs show a markedly low potential at j=10 mA/cm^(2),small Tafel slopes and extremely high stability for ***,the Fe/Fe_(3)C-N-CNTs-based Zn-air batteries demonstrate high power density of 183 mW/cm^(2)and robust charge/discharge *** is revealed that the spatial confinement effect can impede the aggregation and corrosion of Fe/Fe_(3)C ***,Fe/Fe_(3)C and Fe-Nx play synergistic effect on boosting the ORR/OER activity,which provides an important guideline for construction of inexpensive nonprecious metal-carbon hybrid nanomaterials.

关键词： Patial confinement Nonprecious metal Bifunctional Oxygen electrocatalyst Synergistic effect Zn-air batterie

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Covalent Modification of Hemp Seed Polyphenols Improves the Structural and Functional Properties of the Main Hemp Seed Protein Fraction

SSRN

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SSRN 2023年

作者： Xu, Peng-Wei Yuan, Xiao-Fan Zhao, Bing State Key Laboratory of Biochemical Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing100190 China University of Chinese Academy of Sciences Beijing100049 China

In this study, conjugate complexes between the main hemp seed protein fraction, e.g., albumin (ALB) and globulin (GLB), and hemp seed polyphenols (HSP) were prepared through alkalinity grafting modification. The effects of covalent modification with different HSP concentrations (1-5%, w/w) on the structural properties of the protein were studied. The increase in polyphenol grafting equivalent;the decrease in free amino content, sulfhydryl group content, and intrinsic fluorescence intensity;and the change in surface hydrophobicity confirmed the protein-polyphenols covalent interaction enhanced with the HSP content from 1% to 5%. Moreover, UV-visible spectrum, SDS-PAGE, FTIR, and CD analysis showed that the secondary and tertiary structures of the ALB/GLB modified by 5% HSP was changed. Furthermore, ALB/GLB modified by 5% HSP had more excellent antioxidant activities, emulsifying capacity, digestibility, but lower solubility than the corresponding ALB/GLB. These results suggested that the protein-polyphenols conjugate complexes may be used in food formulations as functional ingredients. © 2023, The Authors. All rights reserved.

关键词： Proteins

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Mechanism of Iron Ions Redox Cycle on the Anaerobic Degradation of 3, 5, 6-Trichloro-2-Pyridinol by Pseudomonas Aeruginosa D-2

SSRN

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SSRN 2025年

作者： Lv, Xueru Zhang, Lingping Zhang, Lele Gao, Xijun Ding, Xiaoke Wang, Xueting Jia, Nan Li, Wei Li, Jie Song, Caihong Zhang, Chen Wang, Shenghui College of Agriculture and Biology Liaocheng University Liaocheng252059 China Scbool of Pharmaceutical Sciences and Food Engineering Liaocheng University Liaocheng252059 China State Key Laboratory of Biochemical Engineering Institute of Process Engineering Chinese Academy of Sciences Beijing100190 China

The sluggish metabolism of 3,5,6-trichloro-2-pyridinol (TCP) in anoxic or anaerobic environments represents a significant challenge in bioremediation of TCP-contaminated habitats. Although the promotion of anaerobic biodegradation by insoluble iron minerals has been recognized, the impact and mechanisms of soluble iron ions remain poorly understood. Herein, the soluble Fe(II) was firstly incorporated into the anaerobic biodegradation of TCP by Pseudomonas aeruginosa D-2 and its response of TCP metabolism to the introduction of Fe(II) were investigated. The Fe(II) ion significantly enhanced the anaerobic biodegradation of TCP (50 mg/L) with the removal efficiency increasing from 50.24% to 80.32% within 72 h, along with noticeable cellular proliferation. Interestingly, this process was accompanied by a dynamic equilibrium transformation between Fe(II) and Fe(III). Further study revealed TCP's dechlorination metabolite was substantially degraded by strain D-2 coexisting with Fe(III), concurrently with the reduction of Fe(III) to Fe(II). These findings suggested the iron ions redox cycle significantly promoted the anaerobic biodegradation of TCP. Moreover, metabolomics analysis conformed strain D-2 could perform anaerobic reductive dechlorination of TCP. More importantly, genomic and transcriptomic analyses first revealed Fe(II) markedly enriched energy metabolism pathways, such as TCA cycle and oxidative phosphorylation, and obviously up-regulated some iron-dependent enzymes crucial for ring-cleaving and energy metabolism. These up-regulation may provide ample energy (ATP) and reducing power for the cell growth and the initiation of reductive dechlorination of TCP. This study revealed the effects and mechanism for promoting TCP anaerobic respiration activity by the iron ions redox cycle, acting as an 'energy buffer, in bioremediation of halogenated aromatic contaminants. © 2025, The Authors. All rights reserved.

关键词： Dechlorination

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