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Effects of Heterologously Overexpressing PIP5K-Family genes in Arabidopsis on Inflorescence Development

Phyton-International Journal of Experimental Botany 2024年第1期93卷 97-117页

作者： Mingda Yin Rui Luo Tana Liang Qi Wen Xiaotian Liang Yanpeng Wen Xuemei Hu Zhiyan Wang Chang Gao Wenjing Ren Fenglan Huang College of Life Science and Food Engineering Inner Mongolia Minzu UniversityTongliao028000China Key Laboratory of Castor Breeding of the State Ethnic Affairs Commission Inner Mongolia Minzu UniversityTongliao028000China Inner Mongolia Industrial Engineering Research Center of Universities for Castor Inner Mongolia Minzu UniversityTongliao028043China Inner Mongolia Key Laboratory of Castor Breeding and Comprehensive Utilization Inner Mongolia Minzu UniversityTongliao028000China Inner Mongolia Engineering Research Center of Industrial Technology Innovation of Castor Inner Mongolia Minzu UniversityTongliao028000China

Castor is one of the top 10 oil crops in the world and has extremely valuable *** inflorescences directly affect yield,so the study of inflorescence development is very important in increasing castor *** previous studies have shown that the PIP5K gene family(PIP5Ks)is associated with inflorescence *** this study,to determine the function of each PIP5K gene in castor,a female Lm-type castor line,aLmAB2,was used to determine the relative expression levels of the PIP5Ks in castor *** PIP5K genes were heterologously overexpressed in Arabidopsis thaliana,the relative expression of each gene and the effect on plants was determined in ***,and the relationships among the PIP5Ks in castor were *** expression levels of the PIP5Ks in the female Lm-type castor line aLmAB2 were *** relative expression levels of the PIP5K9 and PIP5K11 genes were high(p<0.05)in isofemale inflorescences,and those of PIP5K1,PIP5K2,PIP5K6,and PIP5K8 were high(p<0.05)in female inflorescences but low(p<0.05)in bisexual *** PIP5Ks were heterologously overexpressed in ***,and T3-generation plants with stable genetic resistance,i.e.,AT-PIP5K^(+)plants(AT-PIP5K1^(+),AT-PIP5K2^(+),AT-PIP5K6^(+),AT-PIP5K8^(+),AT-PIP5K9^(+),and ATPIP5K11^(+) plants),were *** tests of the AT-PIP5K+plants showed that the growth of the main stem was significantly delayed in AT-PIP5K+plants compared with Columbia wild-type(WT)*** plants;the PIP5K1 and PIP5K2 genes promoted lateral stem growth and flower and silique development;and the PIP5K6,PIP5K8,PIP5K9 and PIP5K11 genes inhibited lateral stem growth and flower and silique *** correlations among PIP5Ks in castor suggest that there may be a synergistic relationship among PIP5K1,PIP5K2,and PIP5K6 in castor inflorescences,and PIP5K8,PIP5K9,and PIP5K11 are complementary to the other three genes.

关键词： Castor inflorescence development PIP5K gene family gene overexpression

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Modeling viral evolution:A novel SIRSVIDE framework with application to SARS-CoV-2 dynamics

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hLife 2024年第5期2卷 227-245页

作者： Kaichun Jin Xiaolu Tang Zhaohui Qian Zhiqiang Wu Zifeng Yang Tao Qian Chitin Hon Jian Lu State Key Laboratory of Protein and Plant Gene Research Center for BioinformaticsSchool of Life SciencesPeking UniversityBeijingChina NHC Key Laboratory of Systems Biology of Pathogens Institute of Pathogen BiologyChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingChina State Key Laboratory of Respiratory Disease National Clinical Research Center for Respiratory DiseaseGuangzhou Institute of Respiratory HealthThe First Affiliated Hospital of Guangzhou Medical UniversityGuangdongChina Respiratory Disease AI Laboratory on Epidemic and Medical Big Data Instrument Applications Department of Engineering ScienceFaculty of Innovation EngineeringMacao University of Science and TechnologyMacaoChina Guangzhou Key Laboratory for Clinical Rapid Diagnosis and Early Warning of Infectious Diseases KingMed School of Laboratory MedicineGuangzhou Medical UniversityGuangdongChina Macao Center for Mathematical Sciences Macao University of Science and TechnologyMacaoChina Department of Engineering Science Faculty of Innovation EngineeringMacao University of Science and TechnologyMacaoChina

Understanding evolutionary trends in emerging viruses,such as severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),is crucial for effective public health management and ***,extensive debates have arisen concerning viral evolutionary trends,particularly the interplay between transmissibility,pathogenicity,and immune *** this context,we have developed a novel computational model named SIRSVIDE(Susceptible-Infected-Recovered-Susceptible-Variation-Immune Decay-Immune Escape)to simulate the transmission and evolutionary dynamics of viral *** simulation results indicate that under conditions of high mutation rates,elevated transmission rates,and larger susceptible host populations,viral populations exhibit prolonged increases in transmissibility and immune escape,accompanied by reductions in pathogenicity and noticeable short-term fluctua***,when the total susceptible population size and mutation rate decrease,substantial uncertainty in the evolutionary trends of viral populations becomes *** summary,the SIRSVIDE model establishes a comprehensive framework for generating both short-and long-term viral epidemiological and evolutionary *** simulation outcomes align with existing evidence indicating that SARS-CoV-2 is undergoing selection for heightened transmissibility,decreased pathogenicity,and enhanced immune ***,the model sheds light on the possible evolutionary dynamics of other viruses.

关键词： viral evolution severe acute respiratory syndrome coronavirus 2(SARS-CoV-2) transmissibility-pathogenicity trade-off immune escape

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GSH-responsive camptothecin prodrug-based hybrid micellar nanoparticles enable antitumor chemo-immunotherapy by PD-L1 knockdown

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Nano research 2023年第1期16卷 834-848页

作者： Xi Tan Hong Zhou Chenhui Wang Xuhan Liu Xiangliang Yang Wei Liu College of Life Science and Technology Huazhong University of Science and TechnologyWuhan 430074China The Key Laboratory for Human Disease Gene Study of Sichuan Province and the Department of Laboratory Medicine Sichuan Provincial People’s HospitalUniversity of Electronic Science and Technology of ChinaChengdu 611731China Department of Emergency Medicine Shenzhen University General HospitalShenzhen University Clinical Medical AcademyShenzhen 518060China National Engineering Research Center for Nanomedicine Huazhong University of Science and TechnologyWuhan 430074China

The combinational chemo-immunotherapy as a novel treatment strategy has been widely studied and applied in clinic to enhance antitumor therapeutic efficacy and relieve side *** interference(RNAi)targeting PD-L1 via inhibiting novo production of PD-L1 will overcome the innate and adaptive PD-L1 expression during chemotherapy,thus enable sustained and efficient immune checkpoint blockade(ICB)to active antitumor immune ***,we designed a glutathione(GSH)-responsive camptothecin(CPT)prodrug-based hybrid micellar nanoparticles(siPD-L1@HM-CPT)to achieve synergistic antitumor chemoimmunotherapy by PD-L1 ***-L1@HM-CPT derived from the one-step loading PD-L1 siRNA(siPD-L1)into the CPT prodrug-based hybrid micelles(HM-CPT)which were co-assembled from biodegradable polyphosphoesters-based prodrug CPT-ss-PAEEP15 and stabilizer DSPE-PEG,showed high loading efficiency,GSH-responsive drug release,and excellent stability and ***-L1@HM-CPT achieved simultaneously the co-delivery of CPT and siPD-L1 in vitro and in vivo,high accumulation at the tumor sites,and rapid intracellular release to promote antitumor efficacy via sensitizing CPT chemotherapy,inducing strong immunogenic cell death(ICD)and sustained ICB to improve intratumoral CD8+T cells *** addition,the antitumor immunity response limited by the differentiated immunogenicity,intrinsic PD-L1 expression,and intracellular GSH level was facilitated by efficient ICD and ICB from silencing PD-L1 and synergistic CPT chemosensitization in our experimental B16-F10 and 4T1 tumor *** study might offer a perspective on designing novel co-delivery nanoparticles by convenient and controllable preparation for antitumor chemo-immunotherapy.

关键词： camptothecin prodrug hybrid micellar nanoparticles glutathione(GSH)-responsive PD-L1 knockdown chemoimmunotherapy

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Strigolactone-gibberellin crosstalk mediated by a distant silencer fine-tunes plant height in upland cotton

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Molecular Plant 2024年第10期17卷 1539-1557页

作者： Zailong Tian Baojun Chen Hongge Li Xinxin Pei Yaru Sun Gaofei Sun Zhaoe Pan Panhong Dai Xu Gao Xiaoli Geng Zhen Peng Yinhua Jia Daowu Hu Liru Wang Baoyin Pang Ai Zhang Xiongming Du Shoupu He State Key Laboratory of Cotton Bio-breeding and Integrated Utilization Institute of Cotton ResearchChinese Academy of Agricultural SciencesAnyangChina Zhengzhou Research Base State Key Laboratory of Cotton Bio-breeding and Integrated UtilizationSchool of Agricultural SciencesZhengzhou UniversityZhengzhouChina National Nanfan Research Institute(Sanya) Chinese Academy of Agricultural SciencesSanyaHainanChina School of Computer Science&Information Engineering Anyang Institute of TechnologyAnyangChina National Supercomputing Center in Zhengzhou Zhengzhou UniversityZhengzhouChina

Optimal plant height is crucial in modern agriculture, influencing lodging resistance and facilitating mechanized crop production. Upland cotton (Gossypium hirsutum) is the most important fiber crop globally;however, the genetic basis underlying plant height remains largely unexplored. In this study, we conducted a genome-wide association study to identify a major locus controlling plant height (PH1) in upland cotton. This locus encodes gibberellin 2-oxidase 1A (GhPH1) and features a 1133-bp structural variation (PAVPH1) located approximately 16 kb upstream. The presence or absence of PAVPH1 influences the expression of GhPH1, thereby affecting plant height. Further analysis revealed that a gibberellin-regulating transcription factor (GhGARF) recognizes and binds to a specific CATTTG motif in both the GhPH1 promoter and PAVPH1. This interaction downregulates GhPH1, indicating that PAVPH1 functions as a distant upstream silencer. Intriguingly, we found that DWARF53 (D53), a key repressor of the strigolactone (SL) signaling pathway, directly interacts with GhGARF to inhibit its binding to targets. Moreover, we identified a previously unrecognized gibberellin-SL crosstalk mechanism mediated by the GhD53-GhGARF-GhPH1/PAVPH1 module, which is crucial for regulating plant height in upland cotton. These findings shed light on the genetic basis and gene interaction network underlying plant height, providing valuable insights for the development of semi-dwarf cotton varieties through precise modulation of GhPH1 expression.

关键词： plant height gibberellin 2-oxidase 1A silencer phytohormone crosstalk

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The role of PilU in the surface behaviors of Pseudomonas aeruginosa

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mLife 2025年第1期4卷 83-95页

作者： Jingchao Zhang Yan Luo Yiwu Zong Shangping Lu Yi Shi Fan Jin Kun Zhao Center for Medical Genetics Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengduChina Key Laboratory of SystemsBioengineering(Ministry of Education) School of Chemical Engineering and TechnologyFrontiers Science Center for Synthetic BiologyTianjin UniversityTianjinChina Guangzhou General Institute of Medical Research GuangzhouChina School of Life Science and Technology University of Electronic Science and Technology of ChinaChengduChina The Sichuan Provincial Key Laboratory for Human Disease Gene Study The Institute of Laboratory MedicineSichuan Provincial People's HospitalUniversityof Electronic Science and Technology of ChinaChengduChina CAS Key Laboratory of Quantitative Engineering Biology Shenzhen Institute of Synthetic BiologyShenzhenInstitutes of Advanced TechnologyChinese Academy of SciencesShenzhenChina Institute of Fundamental and Frontier Sciences University of Electronic Science andTechnology of ChinaChengduChina

In Pseudomonas aeruginosa,the dynamic activity of type IV pilus(TFP)is essential for various bacterial *** PilU isconsidered a homolog of the TFP disassembling motor PilT,its speciﬁc roles remain *** pilus visualization andsingle-cell tracking techniques,we characterized TFP dynamics and surface behaviors in wild-type andΔpilU *** thatΔpilU cells displayed increased TFP numbers but reduced cell movement and delayed microcolony ***,beyond affecting the twitching motility,ΔpilU cells formed a thick multilayered colony edge on semi-solid sur-faces,slowing colony ***-cell collision responses changed from touch-turn dominance in wild type to touch-upright dominance inΔpilU,affecting colony morphology and ***ﬁndings expand our understanding of PilU'sphysiological roles and provide potential targets for developing strategies to control *** bioﬁlm formation andvirulence.

关键词： colony expansion pilU Pseudomonas aeruginosa twitching type IV pili

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Insights into chemoautotrophic traits of a prevalent bacterial phylum CSP1-3, herein Sysuimicrobiota

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National Science Review 2024年第11期11卷 61-75页

作者： Lan Liu Zheng-Han Lian Ai-Ping Lv Nimaichand Salam Jian-Chao Zhang Meng-Meng Li Wei-Min Sun Sha Tan Zhen-Hao Luo Lei Gao Yang Yuan Yu-Zhen Ming Yu-Ting Ou Yang Yu-Xian Li Ze-Tao Liu Chao-Jian Hu Ying Chen Zheng-Shuang Hua Wen-Sheng Shu Brian P.Hedlund Wen-Jun Li Jian-Yu Jiao State Key Laboratory of Biocontrol Guangdong Provincial Key Laboratory of Plant Stress Biology and Southern Marine Science and Engineering Guangdong Laboratory ZhuhaiSchool of Life Sciences Sun Yat-Sen University National Agri-Food Biotechnology Institute School of Earth System Science Institute of Surface-Earth System Science Tianjin University National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China Guangdong Key Laboratory of Integrated Agro-Environmental Pollution Control and ManagementInstitute of Eco-Environmental and Soil SciencesGuangdong Academy of Sciences State Key Laboratory of Desert and Oasis Ecology Key Laboratory of Ecological Safety and Sustainable Development in Arid Lands Xinjiang Institute of Ecology and GeographyChinese Academy of Sciences Chinese Academy of Sciences Key Laboratory of Urban Pollutant Conversion Department of Environmental Science and EngineeringUniversity of Science and Technology of China Institute of Ecological Science Guangzhou Key Laboratory of Subtropical Biodiversity and BiomonitoringGuangdong Provincial Key Laboratory of Biotechnology for Plant DevelopmentSchool of Life Sciences South China Normal University Guangdong Provincial Key Laboratory of Chemical Pollution South China Normal University School of Life Sciences University of Nevada Las Vegas Nevada Institute of Personalized Medicine University of Nevada Las Vegas

Candidate bacterial phylum CSP1-3 has not been cultivated and is poorly understood. Here, we analyzed 112 CSP1-3 metagenome-assembled genomes and showed they are likely facultative anaerobes, with 3 of 5 families encoding autotrophy through the reductive glycine pathway(RGP), Wood–Ljungdahl pathway(WLP) or Calvin-Benson-Bassham(CBB), with hydrogen or sulfide as electron donors. Chemoautotrophic enrichments from hot spring sediments and fluorescence in situ hybridization revealed enrichment of six CSP1-3 genera, and both transcribed genes and DNA-stable isotope probing were consistent with proposed chemoautotrophic metabolisms. Ancestral state reconstructions showed that the ancestors of phylum CSP1-3 may have been acetogens that were autotrophic via the RGP, whereas the WLP and CBB were acquired by horizontal gene transfer. Our results reveal that CSP1-3 is a widely distributed phylum with the potential to contribute to the cycling of carbon, sulfur and nitrogen. The name Sysuimicrobiota phy. nov. is proposed.

关键词： Sysuimicrobiota phy. nov facultative anaerobes chemoautotrophic metabolisms

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Consistency,distinction,and potential metabolic crosstalk of nitrogen mobilization-related genes in silk production and silk gland biology

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Zoological research 2025年第2期46卷 446-458页

作者： Mei-Yan Yi Xu Yang Man Wang Jing-Wei Chen Jia-Hao Xiang Li-Jun Xiang Lan-Sa Qian Dong-Bin Chen Yong-Ping Huang Xiao-Ling Tong Zu-Lian Liu Hui Xiang Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouGuangdong 510631China Key Laboratory of Insect Developmental and Evolutionary Biology Center for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghai 200032China University of Chinese Academy of Sciences Beijing 100049China School of Environmental Science and Engineering Shanghai Jiao Tong UniversityShanghai 200240China State Key Laboratory of Resource Insects Key Laboratory for Sericulture Biology and Genetic BreedingMinistry of Agriculture and Rural AffairsCollege of SericultureTextile and Biomass SciencesSouthwest UniversityChongqing 400715China

The domesticated silkworm(Bombyx mori)has evolved a highly efficient nitrogen utilization system to support silk *** silk glands play a pleiotropic role in sequestering nitrogen resources for silk synthesis,mitigating aminoacidemia by assimilating free amino acids,and reallocating nitrogen during metamorphosis through programmed cell ***,the specific functions of nitrogen metabolism-related genes in this process remain *** CRISPR/Cas9-based gene editing,mutations were generated in glutamine synthetase(GS),glutamate synthetase(GOGAT),asparagine synthetase(AS),glutamate dehydrogenase(GDH)and glutamate oxaloacetate transaminase 1(GOT1).Disruption of GS,GOGAT,and AS consistently reduced silkworm cocoon and pupal weight and significantly down-regulated silk protein gene transcription,whereas GOT1 mutation had no such *** mutants exhibited abnormally enlarged silk glands,whereas GS and AS mutants showed delayed programmed cell death in the silk *** contrast,GOT1 mutants displayed normal silk gland morphology but were consistently *** of GS,GOGAT,and AS led to more extensive transcriptional changes,including altered expression of transcription factors in the silk glands,compared with GOT1 *** GS and GOGAT mutants exhibited up-regulation of AS and GDH,while only GOGAT mutants displayed elevated AS enzymatic activity,suggesting that GOGAT may compete with AS for glutamine in the silk glands to support silk protein *** mutants showed significantly elevated GOT activity and up-regulation of several metabolic pathways,indicating that AS may functionally interact with GOT in regulating both silk gland development and programmed cell death during metamorphosis.

关键词： Silkworm Nitrogen mobilization Silk gland Metamorphosis

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Homeodomain proteins POU-M2, antennapedia and abdominal-B are involved in regulation of the segment-specific expression of the clip-domain serine protease gene CLIP13 in the silkworm, Bombyx mori

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Insect Science 2022年第1期29卷 111-127页

作者： Huawei Liu Jingya Heng Luoling Wang Youshan Li Xin Tang Xuan Huang Qingyou Xia Ping Zhao State Key Laboratory of Silkworm Genome Biology Southwest UniversityChongqing400716China Biological Science Research Center Southwest UniversityChongqing400716China Chongqing Key Laboratory of Sericultural Science Chongqing Engineering and Technology Research Center for Novel Silk MaterialsSouthwest UniversityChongqing400716China College of Biological Science and Engineering Shaanxi University of TechnologyHanzhongShaanxi Province723001China

Clip-domain serine proteases (CLIPs) play important roles in insect innate immunity and development. Our previous studies indicated that CLIP13, an epidermis-specific gene, was involved in cuticle remodeling during molting and metamorphosis in the silkworm, Bombyx mori. However, the transcriptional regulatory mechanism and regulatory pathways of CLIP13 remained unclear. In the present study, we investigated CLIP13 expression and the regulation pathway controlled by 20-hydroxyecdysone (20E) in the silkworm. At the transcriptional level, expression of CLIP13 exhibited pronounced spatial and temporal specificity in different regions of the epidermis;homeodomain transcription factors POU-M2, antennapedia (Antp), and abdominal-B (Abd-B) showed similar expression change trends as CLIP13 in the head capsule, thorax, and abdomen, respectively. Furthermore, results of cell transfection assays, electrophoretic mobility shift assays, and chromatin immunoprecipitation demonstrated that POU-M2, Antp, and Abd-B were involved in the transcriptional regulation of CLIP13 by directly binding to their cis-response elements in CLIP13 promoter. RNA interference-mediated silencing of POU-M2, Antp, and Abd-B led to a decrease of CLIP13 expression in the head capsule, the epidermis of the 1st to 3rd thoracic segments and the 7th to 10th abdominal segments, respectively. Consistent with CLIP13, 20E treatment significantly upregulated expression of POU-M2, Antp, and Abd-B in the silkworm epidermis. Taken together, these data suggest that 20E positively regulates transcription of CLIP13 via homeodomain proteins POU-M2, Antp, and Abd-B in different regions of the silkworm epidermis during metamorphosis, thus affecting the molting process. Our findings provide new insight into the functions of homeodomain transcription factors in insect molting.

关键词： Bombyx mori clip-domain serine protease epidermis homeotic gene POU-M2 20-hydroxyecdysone Bombyx mori

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Integrated microfluidic devices for in vitro diagnostics at point of care

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Aggregate 2022年第5期3卷 130-143页

作者： Dan Liu YingWang Xingrui Li Mengmeng Li Qiaoyi Wu Yanling Song Zhi Zhu Chaoyong Yang Engineering Research Centre of Molecular Medicine of Ministry of Education Key Laboratory of Fujian Molecular MedicineKey Laboratory of Precision Medicine and Molecular Diagnosis of Fujian UniversitiesKey Laboratory of Xiamen Marine and Gene DrugsSchool of Biomedical SciencesHuaqiao UniversityFujianChina Xinjiang Key Laboratory of Biological Resources and Genetic Engineering College of Life Science and TechnologyXinjiang UniversityUrumqiChina The Department of Emergency Surgery The First Affiliated Hospital of Fujian Medical UniversityFujianChina The MOE Key Laboratory of Spectrochemical Analysis and Instrumentation The Key Laboratory of Chemical Biology of Fujian ProvinceState Key Laboratory of Physical Chemistry of Solid SurfacesCollaborative Innovation Center of Chemistry for Energy MaterialsDepartment of Chemical BiologyCollege of Chemistry and Chemical EngineeringXiamen UniversityFujianChina

Given the continuous and growing demand for point of care(POC)diagnostic tests,attention has been shifted toward integration and miniaturization of laboratory protocols into“sample-in-answer-out”*** technologies have been considered an ideal solution to address the requirements of POC diagnostics since many laboratory functions can be miniaturized and incorporated onto a single integrated *** this review,we summarize the advances of integrated microfluidic devices for POC diagnostics in the last 3 ***,we summarize current materials used for microfluidic chip fabrication,discuss the innovation of versatile integrated microfluidic devices,especially the strategies for simplifying sample preparation in manual or self-driven systems,and new detection methods of microfluidic *** addition,we describe new integrated microfluidic devices for POC diagnostics of protein-targeted immunodiagnostics,nucleic acid molecular tests,and small molecule metabolites *** also provide future perspectives and current challenges for clinical translation and commercialization of these microfluidic technologies.

关键词： in vitro diagnostics microﬂuidic devices point of care

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MALE STERILITY 3 encodes a plant homeodomain-finger protein for male fertility in soybean

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Journal of Integrative Plant Biology 2022年第5期64卷 1076-1086页

作者： Jingjing Hou Weiwei Fan Ruirui Ma Bing Li Zhihui Yuan Wenxuan Huang Yueying Wu Quan Hu Chunjing Lin Xingqi Zhao Bao Peng Limei Zhao Chunbao Zhang Lianjun Sun State Key Laboratory of Agrobiotechnology Beijing Key Laboratory for Crop Genetic Improvementand College of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China Soybean Research Institute the National Engineering Research Center for SoybeanJilin Academy of Agricultural SciencesChangchun 130033China Jiamusi Branch of Heilongjiang Academy of Agricultural Sciences Jiamusi 154007China

Male-sterile plants are used in hybrid breeding to improve yield in soybean(Glycine max(L.)Merr.).Developing the capability to alter fertility under different environmental conditions could broaden germplasm resources and simplify hybrid ***,molecular mechanisms potentially underlying such a system in soybean were ***,using positional cloning,we identified a gene,MALE STERILITY 3(MS3),which encodes a nuclear-localized protein containing a plant homeodomain(PHD)-finger domain.A spontaneous mutation in ms3 causing premature termination of MS3 translation and partial loss of the *** analysis indicated that MS3 knockout resulted in nonfunctional pollen and no selfpollinated pods,and RNA-seq analysis revealed that MS3 affects the expression of genes associated with carbohydrate ***,the fertility of mutant ms3 can restore under longd *** mutant could thus be used to create a new,more stable photoperiod-sensitive genic male sterility line for two-line hybrid seed production,with significant impact on hybrid breeding and production.

关键词： fertility restoration male sterililty PHD-finger photoperiod soybean

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