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Microbial fermentation of blue honeysuckle(Lonicera caerulea L.)polyphenol promotes insulin secretion and intervenes in obesity by promoting short-chain fatty acids production

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Food Science and Human Wellness 2025年第4期14卷 1449-1468页

作者： Fangyi Pei Xinbo Cao Yanxin Ren Liangyang Mao Yueyi Liu Jingping Ge Wenxiang Ping Engineering Research Center of Agricultural Microbiology Technology Ministry of Education&Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region&Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region&Key Laboratory of MicrobiologyCollege of Heilongjiang Province&School of Life SciencesHeilongjiang UniversityHarbin 150080China Office of Academic Research Qiqihar Medical UniversityQiqihar 161006China

During obesity treatment,clinicians have been burdened by the ever-increasing number of patients and the side effects of drug *** new plant-derived active ingredients as an intervention for obesity has become a research *** this study,Plackett-Burman and Central Composite Design experiments,plant-wide target metabolomics technology,simulated human colon fermentation test,and cell experiment in vitro were successively used to investigate the effects of fermented and unfermented polyphenols(FP and UP,respectively)of blue honeysuckle on *** the optimization of the response surface method,polyphenol content((1878.08±14.53)mg/100 g)increased by 2.63 times after Lactobacillus rhamnosus 6224 and Saccharomyces cerevisiae W5 mixed *** were 23 significantly different metabolites were observed in FP and *** them,7 different metabolites were significantly and positively correlated with short-chain fatty acids(SCFAs),whereas negatively correlated with lipopolysaccharides(LPS).The contents of these 7 metabolites were significantly increased in ***,the effects of FP and UP on the SCFA biosynthesis pathway were *** experiments revealed that polyphenols can significantly promote the secretion of gastrointestinal endogenous hormones and insulin and inhibit interleukin-6 *** regression analysis revealed SCFAs had a positive regulatory effect on glycolipid metabolism,whereas LPS had a negative ***,these crucial findings provide an economical and simple method for obtaining blue honeysuckle polyphenols and laying a theoretical foundation for its application in the therapeutic intervention against obesity.

关键词： Obesity Blue honeysuckle Polyphenol Short-chain fatty acids In vitro fecal fermentation

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Development of an Agrobacterium tumefaciens-mediated transformation system for somatic embryos and transcriptome analysis of LcMYB1's inhibitory effect on somatic embryogenesis in Litchi chinensis

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Journal of Integrative Agriculture 2025年第2期24卷 594-609页

作者： Yaqi Qin Bo Zhang Xueliang Luo Shiqian Wang Jiaxin Fu Zhike Zhang Yonghua Qin Jietang Zhao Guibing Hu State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(South China) Ministry of Agriculture and Rural Affairs/Guangdong Litchi Engineering Research CenterCollege of HorticultureSouth China Agricultural UniversityGuangzhou 510642China

Litchi has great economic significance as a global fruit ***,the advancement of litchi functional genomics has encountered substantial obstacles due to its recalcitrance to stable ***,we present an efficacious Agrobacterium tumefaciens-mediated transformation system in somatic embryos of‘Heiye'*** system was developed through the optimization of key variables encompassing explant selection,*** strain delineation,bacterium concentration,infection duration,and infection *** subsequent validation of the transformation technique in litchi was realized through the ectopic expression of LcMYB1,resulting in the generation of transgenic ***,the differentiation of transgenic calli into somatic embryos encountered substantial *** delineate the intricate molecular underpinnings of LcMYB1's inhibitory role in somatic embryo induction,a comprehensive transcriptome analysis was conducted that encompassed embryogenic calli(C),globular embryos(G),and transgenic calli(TC).A total of 1,166 common differentially expressed genes(DEGs)were identified between C-vs.-G and C-vs.-*** Ontology(GO)annotation and Kyoto Encyclopedia of genes and Genomes(KEGG)pathway analysis revealed that these common DEGs were mostly related to plant hormone signal transduction ***,RT-qPCR corroborated the pronounced down-regulation of numerous genes that are associated with somatic embryo induction within the transgenic *** development of this transformation system provides valuable support for functional genomics research in litchi.

关键词： Litchi chinensis A.tumefaciens transformation LcMYB1 somatic embryo

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Genome-wide identification and characterization of the PbrATG family in Pyrus bretschneideri and functional analysis of PbrATG1a in response to Botryosphaeria dothidea

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Horticultural Plant Journal 2024年第2期10卷 327-340页

作者： Yun Wang Xun Sun Zhenwu Zhang Zhihua Xie Kaijie Qi Shaoling Zhang Center of Pear Engineering Technology Research State Key Laboratory of Crop Genetics and Germplasm EnhancementCollege of HorticultureNanjing Agricultural UniversityNanjingJiangsu 210095China

The pear is an economic fruit that is widely planted around the world and is loved by people for its rich nutritional value. Autophagy is a self-protection mechanism in eukaryotes, and its occurrence often accompanied by the degradation of damaged substances in cells and the recycling of nutrients. Autophagy is one of the mechanisms through which plants respond to environmental stress and plays an important role in plant development and stress resistance. Functional studies of autophagy-related genes (ATGs) have been performed on a variety of plant species, but little information is available on the ATG family in pear (Pyrus bretschneideri Rehd). Therefore, we analyzed the evolutionary dynamics and performed a genome-wide characterization of the PbrATG gene family. A total of 28 PbrATG members were *** analysis showed that PbrATGs were more closely related to ATGs of European pear and apple. Evolutionary analysis revealed that whole-genome duplication (WGD) and dispersed duplication events were the main driving forces of PbrATG family *** analysis of different pear tissues showed that all the genes were expressed in different pear tissues, and different PbrATGs are expressed at different times and in different locations. Moreover, all PbrATGs also responded to different abiotic stresses, especially salt and drought stress, which elicited the highest expression levels. Pear seedlings were subsequently infected with Botryosphaeria dothidea (***). The results showed that different PbrATGs had different expression patterns at different infection stages. According to the gene expression data, PbrATG1a was selected as a key autophagy gene for further analysis. Silencing of PbrATG1a reduced the resistance of pear to B. dothidea, which resulted in increased lesions, reactive oxygen species (ROS) contents, antioxidant enzyme activity, and gene expression levels in the silenced pear seedlings after B. dothidea inoculation. In this s

关键词： Autophagy Pear PbrATGs Abiotic stress Botryosphaeria dothidea PbrATG1a

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Titanium dioxide E171 consumption exacerbates Listeria monocytogenes infection in mice

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Food Quality and Safety 2024年第1期8卷 68-80页

作者：滕跃王瑷琳赵东云李国鹏刘泷泽邹悦夏效东 State Key Laboratory of Marine Food Processing and Safety Control National Engineering Research Center of SeafoodSchool of Food Science and TechnologyDalian Polytechnic UniversityDalianChina

The food additive titanium dioxide is commonly utilized to enhance the appearance and flavor of food ***,this substance has been linked to gastrointestinal *** aim of this study was to investigate the impact of dietary exposure of titanium dioxide E171 on Listeria monocytogenes infection in *** were mainly divided into the control,LM group(*** infection),and E171+LM groups(E171 supplementation before *** infection).Pre-exposure to E171 resulted in increased bacterial counts in the liver,spleen,ileum,colon,mesenteric lymph nodes,and feces of mice after *** ***,E171 exposure increased the levels of pro-inflammatory cytokines while attenuating the levels of anti-inflammatory cytokines in mice infected with ***,mice in the E171+LM group exhibited considerably more severe colonic inflammation and worse intestinal barrier function than mice in the LM *** 16S rRNA gene sequencing revealed a shift in the composition of the gut microbiota of mice in the E171+LM group,characterized by a decrease in the relative abundance of Firmicutes and a decrease in the Firmicutes-to-Bacteroidetes *** levels of acetate,butyrate,and isobutyrate were markedly decreased within the cecum of mice in the E171+LM group in comparison to mice in the LM *** conclusion,these results suggest that E171 exposure could exacerbate *** infection in mice,which may provide useful information for future risk assessment of this commonly used food additive.

关键词： E171 L.monocytogenes intestinal barrier function inflammatory responses gut microbiota.

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The use of melittin to enhance transgene expression mediated by recombinant adeno-associated virus serotype 2 vectors both in vitro and in vivo

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Journal of Integrative Medicine 2023年第1期21卷 106-116页

作者： Yi-lin Xie Ji-yao Wang Yun He Xiao-min Yu Qing-yun Zheng Chen Ling Xi-lin Feng Li-qing Zhu State Key Laboratory of Genetic Engineering and Engineering Research Center of Gene Technology(Ministry of Education) School of Life SciencesZhongshan HospitalFudan UniversityShanghai 200438China Department of Clinical Laboratory the First Affiliated Hospital of Wenzhou Medical UniversityWenzhou 325000Zhejiang ProvinceChina Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province Wenzhou 325000Zhejiang ProvinceChina Yantai Fuheng Biological Technology Co. Ltd.Yantai 264006Shandong ProvinceChin

Objective: Melittin, a cell-penetrating peptide, improves the efficiency of many non-viral gene delivery vectors, yet its application in viral vectors has not been well studied. The non-pathogenic recombinant adeno-associated virus(rAAV) vector is an ideal in vivo gene delivery vector. However, its full potential will only be achieved after improvement of its transduction efficiency. To improve the transduction efficiency of rAAV2 vectors, we attempted to develop a melittin-based r AAV2 vector delivery ***: The melittin peptide was inserted into the rAAV2 capsid either in the loop Ⅷ of all viral proteins(VPs) or at the N terminus of VP2. Various r AAV2-gfp or-fluc vectors were subjected to quantitative real-time polymerase chain reaction and Western blot assays to determine their titers and integrity of capsid proteins, respectively. Alternatively, the vectors based on wild-type capsid were pre-incubated with melittin, followed by transduction of cultured cells or tail vein administration of the mixture to C57BL/6 and BALB/c nude mice. In vivo bioluminescence imaging was performed to evaluate the transgene ***: rAAV2 vectors with melittin peptide inserted in the loop Ⅷ of VPs had low transduction efficiency, probably due to dramatically reduced ability to bind to the target cells. Fusing the melittin peptide at the N-terminus of VP2 produced vectors without the VP2 subunit. Interestingly, among the commonly used rAAV vectors, pre-incubation of r AAV2 and rAAV6 vectors with melittin significantly enhanced their transduction efficiency in HEK293 and Huh7 cells in vitro. Melittin also had the ability to increase the rAAV2-mediated transgene expression in mouse liver in vivo. Mechanistically, melittin did not change the vector-receptor interaction. Moreover, cell counting kit-8 assays of cultured cells and serum transaminase levels indicated melittin had little ***: Pre-incubation with melittin, but not insertion of melitt

关键词： TAAV Melittin Capsid engineering Co-administration Transduction efficiency

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Establishment of somatic embryogenesis regeneration system and transcriptome analysis of early somatic embryogenesis in litchi

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Horticultural Plant Journal 2025年第2期11卷 535-547页

作者： Yaqi Qin Bo Zhang Shiqian Wang Wuyan Guo Zhike Zhang Yonghua Qin Jietang Zhao Guibing Hu State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources/Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(South China) Ministry of Agriculture and Rural Affairs/Guangdong Litchi Engineering Research CenterCollege of HorticultureSouth China Agricultural UniversityGuangzhouGuangdong510642China

Litchi chinensis *** an important economic fruit tree in tropical and subtropical ***,the efficiency of plant regeneration via somatic embryogenesis in litchi is typically low due to the poor conversion of embryos to *** purpose of this study was to establish a regeneration system via somatic embryogenesis from immature embryos explants in‘Heiye'cultivar of *** results demonstrated that MS medium supplemented with 2.0 mg L^(-1)2,4-D was optimal for callus *** somatic embryo(SE)induction,MS medium containing0.5 g L^(-1) activated charcoal(AC)was the most effective,while the use of zeatin(ZT)and thidiazuron(TDZ)resulted in abnormal somatic *** rooting and regeneration rate of 2.15%and 17.5%,respectively,were achieved using MS medium supplemented with 0.5 g L^(-1) ***,transcriptome analysis was performed on embryogenic callus(EC),globular embryo(GE),and heart embryo(HE)to explore the molecular mechanisms of early somatic embryogenesis.2,587 common differentially expressed genes(DEGs)between EC_vs_GE and EC_vs_HE were identified,and the expression patterns of these common DEGs were separated into twelve major *** annotation and KEGG pathway analysis revealed that these common DEGs were implicated in plant hormone signal transduction,auxin-activated signaling pathway,and other biological ***,differentially expressed transcription factors were identified,and the function of LcBBM2 which is specifically highly expressed during early somatic embryogenesis was *** of LcBBM2 in tomato promotes callus and shoot ***,this study can provide a theoretical basis and technical support for genetic breeding improvement of litchi.

关键词： Litchi chinensis Somatic embryogenesis Transcriptome LcBBM2

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Deciphering the intricate hierarchical gene regulatory network:unraveling multi-level regulation and modifications driving secondary cell wall formation

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园艺研究（英文） 2024年第2期11卷 299-321页

作者： Zhigang Wei Hairong Wei Engineering Research Center of Agricultural Microbiology Technology Ministhry of Education & Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region & Key Laboratory of Molecular BiologyCollege of Heilongjiang Province & School of Life SciencesHeilongjiang UniversityHarbin 150080China College of Forest Resources and Environmental Science Michigan Technological UniversityHoughtonMI 49931USA

Wood quality is predominantly determined by the amount and the composition of secondary cell walls(SCWs).Consequently,unraveling the molecular regulatory mechanisms governing SCW formation is of paramount importance for genetic engineering aimed at enhancing wood *** SCW formation is known to be governed by a hierarchical gene regulatory network(HGRN),our understanding of how a HGRN operates and regulates the formation of heterogeneous SCWs for plant development and adaption to ever-changing environment remains *** this review,we examined the HGRNs governing SCW formation and highlighted the significant key differences between herbaceous Arabidopsis and woody plant *** clarified many confusions in existing literatures regarding the HGRNs and their orthologous gene names and ***,we revealed many network motifs including feed-forward loops,feed-back loops,and negative and positive autoregulation in the *** also conducted a thorough review of post-transcriptional and post-translational aspects,protein-protein interactions,and epigenetic modifications of the ***,we summarized how the HGRNs respond to environmental factors and cues,influencing SCW biosynthesis through regulatory cascades,including many regulatory chains,wiring regulations,and network ***,we highlighted the future research directions for gaining a further understanding of molecular regulatory mechanisms underlying SCW formation.

关键词： multi-level secondary network cell wall gene deciphering driving formation hierarchical

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TATA-box binding protein-associated factor 2 regulates grain size in rice

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The Crop Journal 2023年第2期11卷 438-446页

作者： Ling Jiang Ning Jiang Zejun Hu Xuejun Sun Xian Xiang Yahui Liu Mingwei Wu Chunming Liu Xiaojin Luo Key Laboratory of Plant Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China College of Life Sciences University of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of Genetic Engineering and MOE Engineering Research Center of Gene Technology School of Life SciencesFudan UniversityShanghai 200438China School of Advanced Agricultural Sciences Peking UniversityBeijing 100871China

Grain size,characterized by a combination of grain length,width,and thickness,is one of the major determinants of yield in *** present study identified TATA-box binding protein-associated factor 2(TAF2)as an essential component regulating transcription and determining grain size in ***-based cloning showed that a G/T substitution in TAF2 resulted in a naturally occurring mutant called reduced grain size and plant height 1(rgh1).The mutants,with weak edited rgh1 alleles,exhibited a small grain phenotype with reduced grain length and width,while the severe knockout mutant(rgh1-2s)was dwarf and completely *** test performed between rgh1 and several edited alleles confirmed that the mutation in TAF2 caused the rgh1 *** staining showed that TAF2 was mainly expressed in the vascular bundles of roots,stems,leaves,and *** cytological analysis revealed that reduced cell division in the glumes resulted in the small grain phenotype of *** RNA-sequencing detected altered expression of genes involved in the basic biological processes in rgh1 *** findings provide novel insights into the TAF2-mediated genetic mechanism regulating grain size in rice.

关键词： Grain size TAF2 RGH1 Cell division Rice

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Rice FLOURY ENDOSPERM22, encoding a pentatricopeptide repeat protein, is involved in both mitochondrial RNA splicing and editing and is crucial for endosperm development

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Journal of Integrative Plant Biology 2023年第3期65卷 755-771页

作者： Hang Yang Yunlong Wang Yunlu Tian Xuan Teng Zehui Lv Jie Lei Erchao Duan Hui Dong Xue Yang Yuanyan Zhang Yinglun Sun Xiaoli Chen Xiuhao Bao Rongbo Chen Chuanwei Gu Yipeng Zhang Xiaokang Jiang Wenyu Ma Pengcheng Zhang Yi Ji Yu Zhang Yihua Wang Jianmin Wan State Key Laboratory for Crop Genetics and Germplasm Enhancement Jiangsu Plant Gene Engineering Research CenterNanjing Agricultural UniversityNanjing 210095China National Key Facility for Crop Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China

Most of the reported P-type pentatricopeptide repeat(PPR) proteins play roles in organelle RNA stabilization and splicing. However, P-type PPRs involved in both RNA splicing and editing have rarely been reported, and their underlying mechanism remains largely unknown. Here, we report a rice floury endosperm22(flo22) mutant with delayed amyloplast development in endosperm cells. Map-based cloning and complementation tests demonstrated that FLO22 encodes a mitochondrion-localized P-type PPR *** of FLO22 resulting in defective transsplicing of mitochondrial nad1 intron 1 and perhaps causing instability of mature transcripts affected assembly and activity of complex Ⅰ, and mitochondrial morphology and function. RNA-seq analysis showed that expression levels of many genes involved in starch and sucrose metabolism were significantly down-regulated in the flo22mutant compared with the wild type, whereas genes related to oxidative phosphorylation and the tricarboxylic acid cycle were significantly upregulated. In addition to involvement in splicing as a P-type PPR protein, we found that FLO22 interacted with DYW3, a DYW-type PPR protein, and they may function synergistically in mitochondrial RNA editing. The present work indicated that FLO22 plays an important role in endosperm development and plant growth by participating in nad1 maturation and multi-site editing of mitochondrial messager RNA.

关键词： endosperm development Oryza sativa PPR proteins RNA editing RNA splicing

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Multi-omics analysis reveals a novel NFE2L3 variant impairing choroidal vasculature development in high myopia and myopic maculopathy

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国家科学评论（英文版） 2025年第2期12卷 5-8页

作者： Jiangnan He Luyao Ye Hannan Xu Huanjie Yang Juan Shen Menghan Li Shijun Weng Dongyue Jiao Chen Chu Qijun Liao Haidong Zou Jianfeng Zhu Chenji Wang Xun Xu Department of Preventative Ophthalmology Shanghai Eye Diseases Prevention &Treatment Center/Shanghai Eye HospitalSchool of MedicineTongji UniversityNational Clinical Research Center for Eye DiseasesShanghai Engineering Research Center of Precise Diagnosis and Treatment of Eye DiseasesChina Department of Ophthalmology Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai Clinical Research Center for Eye DiseasesShanghai Key Clinical SpecialtyShanghai Key Laboratory of Ocular Fundus DiseasesShanghai Engineering Center for Visual Science and PhotomedicineChina Department of Ophthalmology The Affiliated People's Hospital of Ningbo UniversityChina Department of Ophthalmology Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai Clinical Research Center for Eye DiseasesShanghai Key Clinical SpecialtyShanghai Key Laboratory of Ocular Fundus DiseasesShanghai Engineering Center for Visual Science and PhotomedicineChina BGI Research China College of Life Sciences University of Chinese Academy of SciencesChina BGI Research China Institute of Metagenomics Qingdao-Europe Advanced Institute for Life SciencesBGI ResearchChina State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Institutes of Brain ScienceFudan UniversityChina State Key Laboratory of Genetic Engineering MOE Engineering Research Center of Gene TechnologyShanghai Engineering Research Center of Industrial MicroorganismsSchool of Life SciencesFudan UniversityChina BGI Research China Department of Preventative Ophthalmology Shanghai Eye Diseases Prevention &Treatment Center/Shanghai Eye HospitalSchool of MedicineTongji UniversityNational Clinical Research Center for Eye DiseasesShanghai Engineering Research Center of Precise Diagnosis and Treatment of Eye DiseasesChina Department of Ophthalmology Shanghai General HospitalShanghai Jiao Tong University School of MedicineShanghai Clinical Research Center for Eye DiseasesShanghai Key Clinical Spec

Myopic maculopathy(MM)is a severe complication of high myopia,character-ized by degenerative lesions in the mac-ular retina,choroid and sclera[1].The pooled prevalence of MM is estimated to be 2.1％in the world popula... 详细信息

关键词： development analysis variant novel high choroidal impairing maculopathy multi-omics myopia

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