检索结果-内蒙古大学图书馆

A growth-regulating factor 7(GRF7)-mediated gene regulatory network promotes leaf growth and expansion in sugarcane

The Crop Journal 2024年第2期12卷 422-431页

作者： Qiaoyu Wang Yihan Li Dadong Lin Xiaoxi Feng Yongjun Wang Tianyou Wang Hongyan Ding Jisen Zhang State Key Lab for Conservation and Utilization of Subtropical AgroBiological Resources and Guangxi Key Lab for Saccharum Biology Guangxi UniversityNanning 530005GuangxiChina Center for Genomics and Biotechnology/Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology/Key Laboratory of Saccharum Biology and Genetic Breeding/National Engineering Research Center for Saccharum Fujian Agriculture and Forestry UniversityFuzhou 350002FujianChina

Knowledge of the function of growth-regulating factors(GRFs)in sugarcane(Saccharum officinarum and ***)growth and development could assist breeders in selecting desirable plant ***,limited information about GRFs is available in Saccharum due to their *** this study,22 GRFs were identified in the two species and their conserved domains,gene structures,chromosome location,and synteny were ***7 expression varied among tissues and responded to diurnal ***7-YFP was localized preferentially in the nucleus and appears to act as a transcriptional ***7 positively regulated the size and length of rice leaves,possibly by regulating cell size and plant *** seven potential transcription factors binding to the SsGRF7 promoter in ***,four showed positive expression patterns,and two showed negative expression patterns relative to SsGRF7.

关键词： Expression analysis Growth-regulating factor Leaf development Sugarcane Transcription factors

来源：评论

学校读者我要写书评

暂无评论

Preparation and Characterization of pH-Responsive Charge Reversal Nanocomposite for miRNA Delivery

引用

Journal of Wuhan University of technology(Materials Science) 2024年第4期39卷 1048-1052页

作者：余丹 YE Liyuan LI Binbin MOU Fangzhi 殷义霞 State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of TechnologyWuhan 430070China Hainan Institute Wuhan University of TechnologySanya 572000China Biomedical Materials and Engineering Research Center of Hubei Province Wuhan University of TechnologyWuhan 430070China

pH-responsive charge reversal loaded miRNA nanocomposite was prepared by electrostatic *** morphology,particle size and zeta potential of the nanocomposites were analyzed by transmission electron microscopy and dynamic light *** synthesis of the polymer was analyzed by^(1)*** zeta-potential changes and cellular uptake effects of the nanocomplexes under different pH environments were *** experimental results show that the surface morphology of the nanocomposite is spherical,and the average particle size is about 135 *** the pH value of the solution gradually decreases,the surface charge of the nanocomposite reverses from negative charge to positive charge(from-9.4 to+17.1 mV).Cellular uptake mediated by pH-responsive nanocomposite is selective for tumor cells,and the cellular uptake effect in tumor cells at pH 6.5 was approximately 3 times higher than that at pH *** pH responsive charge reversal nanocomposite has promising application prospects for gene delivery in the weak acid environment of tumors.

关键词： charge conversion siRNA delivery pH responsive cancer therapy

来源：评论

学校读者我要写书评

暂无评论

Fast,simple,efficient Agrobacterium rhizogenes-mediated transformation system to non-heading Chinese cabbage with transgenic roots

引用

Horticultural Plant Journal 2024年第2期10卷 450-460页

作者： Huiyu Wang Yushan Zheng Qian Zhou Ying Li Tongkun Liu Xilin Hou State Key Laboratory of Crop Genetics&Germplasm Enhancement Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(East China)Ministry of Agriculture and Rural Affairs of ChinaEngineering Research Center of Germplasm Enhancement and Utilization of Horticultural CropsMinistry of Education of ChinaNanjing Agricultural UniversityNanjingJiangsu 210095China Nanjing Suman Plasma Engineering Research Institute Nanjing Agricultural UniversityNanjingJiangsu 210095China

Non-heading Chinese cabbage, a variety of Brassica campestris, is an important vegetable crop in the Yangtze River Basin of China. However,the immaturity of its stable transformation system and its low transformation efficiency limit gene function research on non-heading Chinese cabbage. Agrobacterium rhizogenes-mediated(ARM) transgenic technology is a rapid and effective transformation method that has not yet been established for non-heading Chinese cabbage plants. Here, we optimized conventional ARM approaches(one-step and two-step transformation methods) suitable for living non-heading Chinese cabbage plants in nonsterile environments. Transgenic roots in composite non-heading Chinese cabbage plants were identified using phenotypic detection, fluorescence observation, and PCR analysis. The transformation efficiency of a two-step method on four five-day-old non-heading Chinese cabbage seedlings(Suzhouqing, Huangmeigui, Wuyueman, and Sijiu Caixin) was 43.33%-51.09%, whereas using the stout hypocotyl resulted in a transformation efficiency of 54.88% for the 30-day-old Sijiu *** one-step method outperformed the two-step method;the transformation efficiency of different varieties was above 60%, and both methods can be used to obtain transgenic roots for functional studies within one month. Finally, optimized ARM transformation methods can easily,quickly, and effectively produce composite non-heading Chinese cabbage plants with transgenic roots, providing a reliable foundation for gene function research and non-heading Chinese cabbage genetic improvement breeding.

关键词： Agrobacterium rhizogenes Non-heading Chinese cabbage Transgenic roots Composite plant Transformation efficiency

来源：评论

学校读者我要写书评

暂无评论

Insertion sequence transposition activates antimycobacteriophage immunity through an Isr2-silenced lipid metabolism gene island

引用

mLife 2024年第1期3卷 87-100页

作者： Yakun Li Yuyun Wei Xiao Guo Xiaohui Li Lining Lu Lihua Hu Zheng-Guo He State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangxi Research Center for Microbial and Enzyme Engineering TechnologyCollege of Life Scienceand TechnologyGuangxi UniversityNanningChina

Insertion sequences(ISs)exist widely in bacterial genomes,but their roles in the evolution of bacterial antiphage defense remain to be ***,we report that,under the pressure of phage infection,the IS1o96 transposition of Mycobacterium smegmatis into the Isr2 gene can occur at high frequencies,which endows the mutant mycobacterium with a broad-spectrum antiphage ***2 functions as a negative regulator and directly silences expression of a gene island composed of 11 lipid metabolism-related *** complete or partial loss of the gene island leads to a significant decrease of bacteriophage adsorption to the mycobacterium,thus defending against phage ***,a phage that has evolved mutations in two tail-filament genes can re-escape from the Isr2 inactivation-triggered host *** study uncovered a new signaling pathway for activating antimycobacteriophage immunity by Is transposition and provided insight into the natural evolution of bacterial antiphage defense.

关键词： antiphage defense bacteriophage gene island insertion sequence mycobacteria

来源：评论

学校读者我要写书评

暂无评论

Glibenclamide targets MDH2 to relieve aging phenotypes through metabolism-regulated epigenetic modification

引用

Signal Transduction and Targeted Therapy 2025年第3期10卷 1652-1666页

作者： Zhifan Mao Wenwen Liu Rong Zou Ling Sun Shuman Huang Lingyu Wu Liru Chen Jiale Wu Shijie Lu Zhouzhi Song Xie Li Yunyuan Huang Yong Rao Yi-You Huang Baoli Li Zelan Hu Jian Li State Key Laboratory of Bioreactor Engineering Shanghai Frontiers Science Center of Optogenetic Techniques for Cell MetabolismFrontiers Science Center for Materialbiology and Dynamic ChemistryShanghai Key Laboratory of New Drug DesignSchool of PharmacyEast China University of Science and TechnologyShanghai 200237China Key Laboratory of Tropical Biological Resources of Ministry of Education and Hainan Engineering Research Center for Drug Screening and EvaluationSchool of Pharmaceutical SciencesHainan UniversityHaikou 570228China Hubei Key Laboratory of Genetic Regulation and Integrative Biology School of Life SciencesCentral China Normal UniversityWuhanChina Key Laboratory of Xinjiang Phytomedicine Resource and Utilization Ministry of EducationSchool of PharmacyShihezi UniversityShihezi 832003China

Mitochondrial metabolism-regulated epigenetic modification is a driving force of aging and a promising target for therapeutic *** malate dehydrogenase(MDH2),an enzyme in the TCA cycle,was identified as an anti-aging target through activity-based protein profiling in present *** expression level of MDH2 was positively correlated with the cellular senescence in Mdh2 knockdown or overexpression ***(Gli),a classic anti-glycemic drug,was found to inhibit the activity of MDH2 and relieve fibroblast senescence in an MDH2-dependent *** anti-aging effects of Gli were also further validated in vivo,as it extended the lifespan and reduced the frailty index of naturally aged *** specific Mdh2 knockdown eliminated Gli’s beneficial effects in naturally aged mice,reducing p^(16INK4a) expression and hepatic ***,MDH2 inhibition or knockdown disrupted central carbon metabolism,then enhanced the methionine cycle flux,and subsequently promoted histone ***,the tri-methylation of H3K27,identified as a crucial methylation site in reversing cellular senescence,was significantly elevated in hepatic tissues of naturally aged mice with Mdh2 *** together,these findings reveal that MDH2 inhibition or knockdown delays the aging process through metabolic-epigenetic *** research not only identified MDH2 as a potential therapeutic target and Gli as a lead compound for anti-aging drug development,but also shed light on the intricate interplay of metabolism and epigenetic modifications in aging.

关键词： metabolism glibenclamide cellular senescence MDH therapeutic interventionmitochondrial malate dehydrogenase mdh epigenetic modification aging inhibit activ

来源：评论

学校读者我要写书评

暂无评论

BcWRKY25-BcWRKY33A-BcLRP1/BcCOW1 module promotes root development for improved salt tolerance in Bok choy

引用

园艺研究（英文） 2025年第1期12卷 137-148页

作者： Huiyu Wang Yushan Zheng Meiyun Wang Wusheng Liu Ying Li Dong Xiao Tongkun Liu Xilin Hou State Key Laboratory of Crop Genetics & Germplasm Enhancement Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(East China)Ministry of Agriculture and Rural Affairs of ChinaEngineering Research Center of Germplasm Enhancement and Utilization of Horticultural CropsMinistry of Education of ChinaNanjing Agricultural UniversityNo.1 Weigang RoadXuanwu DistrictNanjing 210095China Department of Plant Science and Technology Beijing Key Laboratory of New Technology in Agricultural ApplicationNational Demonstration Center for Experimental Plant Production EducationBeijing University of AgricultureNo.7 Beinong RoadChangping DistrictBeijing 102206China State Key Laboratory of Crop Genetics & Germplasm Enhancement Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(East China)Ministry of Agriculture and Rural Affairs of ChinaEngineering Research Center of Germplasm Enhancement and Utilization of Horticultural CropsMinistry of Education of ChinaNanjing Agricultural UniversityNo.1 Weigang RoadXuanwu DistrictNanjing 210095China Department of Horticultural Science North Carolina State University2101 Hillsborough StreetRaleighNorth Carolina 27607USA State Key Laboratory of Crop Genetics & Germplasm Enhancement Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(East China)Ministry of Agriculture and Rural Affairs of ChinaEngineering Research Center of Germplasm Enhancement and Utilization of Horticultural CropsMinistry of Education of ChinaNanjing Agricultural UniversityNo.1 Weigang RoadXuanwu DistrictNanjing 210095China Nanjing Suman Plasma Engineering Research Institute Nanjing Agricultural UniversityNo.7 Yinian RoadJiangning DistrictNanjing 210095China

Root development is a complex process involving phytohormones and transcription *** previous research has demonstrated that BcWRKY33A is significantly expressed in Bok choy roots under salt stress,and heterologous expression of BcWRKY33A increases salt tolerance and promotes root development in transgenic ***,the precise molecular mechanisms by which BcWRKY33A governs root development remain ***,we investigated the role of BcWRKY33A in both root elongation and root hair formation in transgenic Bok choy *** data indicated that overexpression of BcWRKY33A stimulated root growth and stabilized root hair morphology,while silencing BcWRKY33A prevented primary root elongation and resulted in abnormal root hairs ***,our research uncovered that BcWRKY33A directly binds to the promoters of BcLRP1 and BcCOW1,leading to an upregulation of their *** transgenic Bok choy roots,increased BcLRP1 and BcCOW1 transcript levels improved primary root elongation and root hair formation,***,we pinpointed BcWRKY25 as a NaCl-responsive gene that directly stimulates the expression of BcWRKY33A in response to salt *** results shed light on the regulatory mechanisms governing root development by BcWRKY25-BcWRKY33A-BcLRP1/BcCOW1 module and propose potential strategies for improving salt tolerance in Bok choy.

关键词： development tolerance improved module salt root bccow1 bclrp bcwrky choy

来源：评论

学校读者我要写书评

暂无评论

Activation ofγ-globin expression by a common variant disrupting IKAROS-binding motif inβ-thalassemia

引用

Journal of genetics and Genomics 2025年第2期52卷 157-167页

作者： Hualei Luo Jueheng Wang Lang Qin Xinhua Zhang Hailiang Liu Chao Niu Mengyang Song Congwen Shao Peng Xu Miao Yu Haokun Zhang Yuhua Ye Xiangmin Xu Innovation Center for Diagnostics and Treatment of Thalassemia Nanfang HospitalSouthern Medical UniversityGuangzhouGuangdong 510515China Department of Medical Genetics School of Basic Medical SciencesSouthern Medical UniversityGuangzhouGuangdong 510515China State Key Laboratory of Genetic Engineering School of Life SciencesFudan UniversityShanghai 201114China Department of Pediatrics 923rd Hospital of the People's Liberation ArmyNanningGuangxi 530021China Cyrus Tang Medical Institute National Clinical Research Centre for Hematologic DiseasesCollaborative Innovation Centre of HematologyState Key Laboratory of Radiation Medicine and ProtectionSoochow University SuzhouSuzhouJiangsu 215031China State Key Laboratory of Genetic Engineering MOE Engineering Research Center of Gene TechnologySchool of Life SciencesFudan UniversityShanghai 200438China

Programmed silencing ofγ-globin genes in adult erythropoiesis is mediated by several chromatin remodeling complexes,which determine the stage-specific genome architecture in this *** of cis-or trans-acting mutations contributing to the diverse extent of fetal hemoglobin(Hb F)might illustrate the underlying mechanism ofγ-β-globin ***,we recruit a cohort of 1142β-thalassemia patients and dissect the natural variants in the wholeβ-globin gene cluster through a targeted next-generation sequencing panel.A previously unreported SNP rs7948668,predicted to disrupt the binding motif of IKAROS as a key component of chromatin remodeling complexes,is identified to be significantly associated with higher levels of Hb F and age at ***-editing on this SNP leads to the elevation of Hb F in both HUDEP-2 and primary CD34+cells while the extent of elevation is amplified in the context ofβ-thalassemia mutations,indicating epistasis effects of the SNP in the regulation of Hb ***,we perform ChIP-qPCR and 4C assays to prove that this variant disrupts the binding motif of IKAROS,leading to enhanced competitiveness of HBG promoters to locus control *** study highlights the significance of common regulatory SNPs and provides potential targets for treatingβ-hemoglobinopathy.

关键词： β-thalassemia Fetal hemoglobin Single nucleotidepolymorphism IKAROS Locus control region

来源：评论

学校读者我要写书评

暂无评论

Tetrapyrrole biosynthesis pathway regulates plastid-to-nucleus signaling by controlling plastid gene expression in plants

引用

Plant Communications 2023年第1期4卷 1-12页

作者： Yunlong Wang Yihua Wang Xiaopin Zhu Yulong Ren Hui Dong Erchao Duan Xuan Teng Huanhuan Zhao Rongbo Chen Xiaoli Chen Jie Lei Hang Yang Yunlu Tian Liangming Chen Xi Liu Shijia Liu Ling Jiang Haiyang Wang Jianmin Wan State Key Laboratory for Crop Genetics and Germplasm Enhancement Jiangsu Plant Gene Engineering Research CenterNanjing Agricultural UniversityNanjing 210095P.R.China National Key Facility for Crop Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081P.R.China

Plastid-to-nucleus retrograde signaling coordinates nuclear gene expression with chloroplast developmental status and is essential for the photoautotrophic lifestyle of *** studies have established that tetrapyrrole biosynthesis(TPB)and plastid gene expression(PGE)play essential roles in plastid retrograde signaling during early chloroplast biogenesis;however,their functional relationship remains *** this study,we generated a series of rice TPB-related gun(genome uncoupled)mutants and systematically analyzed their effects on nuclear and plastid gene expression under normal conditions or when subjected to treatments with norflurazon(NF;a noncompetitive inhibitor of carotenoid biosynthesis)and/or lincomycin(Lin;a specific inhibitor of plastid translation).We show that under NF treatment,expression of plastid-encoded polymerase(PEP)-transcribed genes is significantly reduced in the wild type but is derepressed in the TPB-related gun *** further demonstrate that the derepressed expression of PEPtranscribed genes may be caused by increased expression of the PEP core subunit and nuclear-encoded sigma factors and by elevated copy numbers of plastid genome per haploid *** addition,we show that expression of photosynthesis-associated nuclear genes(PhANGs)and PEP-transcribed genes is correlated in the rice TPB-related gun mutants,with or without NF or Lin treatment.A similar correlation between PhANGs and PGE is also observed in the Arabidopsis gun4 and gun5 ***,we show that increased expression of PEP-transcribed plastid genes is necessary for the gun phenotype in NF-treated TPB-related gun ***,we provide evidence that these TPB-related GUN genes act upstream of GUN1 in the regulation of retrograde *** together,our results suggest that the TPB-related GUN genes control retrograde plastid signaling by regulating the PGE-dependent retrograde signaling pathway.

关键词： tetrapyrrole biosynthesis plastid gene expression plastid retrograde signal

来源：评论

学校读者我要写书评

暂无评论

DNA methylation-mediated ROS production contributes to seed abortion in litchi

引用

Molecular Horticulture 2024年第1期4卷 430-444页

作者： Hanhan Xie Yedan Zheng Mengyue Xue Yulian Huang Dawei Qian Minglei Zhao Jianguo Li State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources Guangdong Laboratory for Lingnan Modern AgricultureKey Laboratory of Biology and Genetic Improvement of Horticultural Crops(South China)Ministry of Agriculture and Rural AffairsGuangdong Litchi Engineering Research CenterCollege of HorticultureSouth China Agricultural UniversityGuangzhou 510642China

Although there is increasing evidence suggesting that DNA methylation regulates seed development,the underlying mechanisms remain poorly ***,we aimed to shed light on this by conducting whole-genome bisulfite sequencing using seeds from the large-seeded cultivar’HZ’and the abortive-seeded cultivar’NMC’.Our analysis revealed that the’HZ’seeds exhibited a hypermethylation level compared to the’NMC’***,we found that the genes associated with differentially methylated regions(DMRs)and differentially expressed genes(DEGs)were mainly enriched in the reactive oxygen species(ROS)metabolic *** investigate this further,we conducted nitroblue tetrazolium(NBT)and 2,7-Dichlorodihydrofluorescein(DCF)staining,which demonstrated a significantly higher amount of ROS in the’NMC’seeds compared to the’HZ’***,we identified that the gene LcGPX6,involved in ROS scavenging,exhibited hypermethylation levels and parallelly lower expression levels in’NMC’seeds compared to’HZ’***,the ectopic expression of LcGPX6 in Arabidopsis enhanced ROS scaveng-ing and resulted in lower seed ***,we suggest that DNA methylation-mediated ROS production plays a significant role in seed development in litchi,during which hypermethylation levels of LcGPX6 might repress its expression,resulting in the accumulation of excessive ROS and ultimately leading to seed abortion.

关键词： Seed abortion DNA methylation ROS

来源：评论

学校读者我要写书评

暂无评论

Intracellularly synthesized ssDNA for continuous genome engineering

引用

Trends in Biotechnology 2025年第6期43卷 1356-1370页

作者： Liu, Wenqian Pan, Yingjia Zhang, Yu Dong, Chang Huang, Lei Lian, Jiazhang Key Laboratory of Biomass Chemical Engineering of Ministry of Education and State Key Laboratory of Biobased Transportation Fuel Technology College of Chemical and Biological Engineering Zhejiang University Hangzhou310027 China BGI Research Hangzhou310030 China ZJU-Hangzhou Global Scientific and Technological Innovation Center Zhejiang University Hangzhou310000 China

Despite the prevalence of genome editing tools, there are still some limitations in dynamic and continuous genome editing. In vivo single-stranded DNA (ssDNA)-mediated genome mutation has emerged as a valuable and promising approach for continuous genome editing. In this review, we summarize the various types of intracellular ssDNA production systems and notable achievements in genome engineering in both prokaryotic and eukaryotic cells. We also review progress in the development of applications based on retron-based systems, which have demonstrated significant potential in molecular recording, multiplex genome editing, high-throughput functional variant screening, and gene-specific continuous in vivo evolution. Furthermore, we discuss the major challenges of ssDNA-mediated continuous genome editing and its prospects for future applications. © 2024 Elsevier Ltd

关键词： Genome

来源：评论

学校读者我要写书评

暂无评论

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案：

请选择收藏分类：

通借通还

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：