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Single Cell Protein Detection by Digital Quantification of Amplified Single Molecules

Single Cell Protein Detection by Digital Quantification of A...

第九届全国微全分析系统学术会议、第四届全国微纳尺度生物分离分析学术会议、2014国际微流控芯片与微纳尺度生物分离分析学术会议

作者： Qiangyuan Zhu Lin Qiu Yanan Xu Congcong Ma Wei Jin Qinhan Jin Ying Mu Research Center for Analytical Instrumentation Institute of Cyber-Systems and ControlState Key Laboratory of Industrial Control TechnologyZhejiang UniversityHangzhou 310058China

Very low abundance protein molecular detection,especially for early diagnosis of cancer markers detection is extremely difficult,with the development of single-cell omics in the recent years,there is no a better approach for precise quantification of protein molecules within a single *** intend to develop an novel digital proximity ligation assay chip for single protein molecule detection,which integrates the proximity ligation assay for protein detection with the self-priming compartmentalization digital PCR chip to develop an absolute quantitative method for single protein molecule counting-digital proximity ligation *** method can provide a new tool for single molecule proteins detection of low abundance proteins and absolute quantification of single cell protein molecule *** the advantages of high detection sensitivity,strong specificity,low sample consumption,simple operation,testing equipment common and so on,the digital PLA method not only can detect trace amounts of tumor protein markers in the serum with tiny nonspecific detection,and the number of protein molecules in a single cell by single molecule *** to the existing single molecule enzyme-linked immunoassays detection technology,it is more simple,less sample consumption,better sensitivity and *** the early diagnosis of cancer,the occurrence and development of tumor,drug screening,single cell analysis and identification of cancer stem cells,digital PLA will have important application value and practical value.

关键词： single protein molecule digital PCR single cell Proximity ligation Assay

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New trends in nonlinear control systems and applications

Abstract and Applied Analysis

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Abstract and Applied Analysis 2015年第1期2015卷

作者： Liu, Zhitao Huang, Deqing Xing, Yifan Zhang, Chuanke Wu, Zhengguang Ji, Xiaofu State Key Laboratory of Industrial Control Technology Institute of Cyber-Systems and Control Zhejiang University Hangzhou 310027 China Department of Aeronautics Imperial College London London SW7 2AZ United Kingdom Frick Chemistry Laboratory Princeton University Princeton 08544 NJ United States Department of Electrical Engineering and Electronics University of Liverpool Liverpool L69 3GJ United Kingdom Department of Electrical Engineering School of Electrical and Information Engineering Jiangsu University Zhenjiang 212013 China

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A Partial-state Observer for a Class of MIMO Nonlinear systems

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IFAC Proceedings Volumes 2014年第3期47卷 3676-3681页

作者： Lei Wang Alberto Isidori Hongye Su Weihua Xu State Key Laboratory of Industrial Control Technology Institute of Cyber-Systems and Control Zhejiang University 310027 Hangzhou P.R.China Department of Computer Control and Management Engineering University of Rome “La Sapienza” Rome Italy

In this paper, we consider input-affine invertible MIMO nonlinear systems which can be transformed into a special normal form by means of the structure algorithm. The normal form highlights a partial state, a subset of state variables, which plays in this setting a role similar to that of the outputs and its derivatives in a SISO system. It is shown that, if a system in this class can be asymptotically stabilized by means of a static feedback from that partial state, then semiglobal stabilization can be achieved via dynamic feedback driven by the output of the system. The dynamic feedback in question is based a (non–trivial) extension to MIMO systems of the standard high–gain observer.

关键词： MIMO nonlinear systems structure algorithm normal forms minimum-phase systems nonlinear observers

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Improving the real-time performance of Ethernet for plant automation(EPA) based industrial networks

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Journal of Zhejiang University-Science C(Computers and Electronics) 2013年第6期14卷 433-448页

作者： Li LU Dong-qin FENG Jian CHU Institute of Cyber-Systems and Control Zhejiang University State Key Laboratory of Industrial Control Technology Zhejiang University

Real-time Ethernet(RTE) control systems with critical real-time requirements are called fast real-time(FRT) *** improve the real-time performance of Ethernet for plant automation(EPA),we propose an EPA-FRT *** minimum macrocycle of EPA networks is reduced by redefining the EPA network frame format,and the synchronization process is modified to acquire higher accuracy.A multi-segmented topology with a scheduling scheme is introduced to increase effective bandwidth utilization and reduce protocol overheads,and thus to shorten the communication cycle *** analysis and practical tests on a prototype system show the effectiveness of the proposed scheme,which achieves the best performance at small periodic payload in large scale systems.

关键词： Ethernet for plant automation （EPA） Fast real-time （FRT） system Real-time Ethernet （RTE） Scheduling scheme171 ~ ~l ~nI~ ]Ui^umanf onrla＊ A ~｜ F numh~＊ TDg02

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Spatial Batch Optimal Design Based on Self-Learning Gaussian Process Models for LPCVD Processes

Spatial Batch Optimal Design Based on Self-Learning Gaussian...

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第25届中国过程控制会议

作者： Pei Sun Junghui Chen Lei Xie Xing Cai Shan Lu State Laboratory of Industrial Control Technology Institute of Cyber-Systems and Control Zhejiang University Department of Chemical Engineering Chung-Yuan Christian University

Low pressure chemical vapor deposition(LPCVD)is one of the most important processes during semiconductor ***,experience based control is often used to design this *** few data of thin film thickness on wafers can be available after a long-depositing-time ***,the spatial distribution of temperature makes it hard to control product quality on the multiple *** this work,an optimal design strategy based on the Gaussian process model(GPM)is proposed to control this kind of spatial batch *** the conventional model based design,GPM built up from the data of wafer thicknesses in previous *** it will be employed to predict the thickness of all the wafers on different spatial ***,the predictive uncertainties provided by GPM are also taken into consideration to guide the optimal design of manipulated variables so that the controlling can be more *** effectiveness of the proposed strategy is successfully demonstrated in an LPCVD process.

关键词： LPCVD,Spatial Distribution,Gaussian Process Model,Optimal Design

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Robust Adaptive Backstepping control of Second-order Nonlinear systems with Non-triangular Structure Uncertainties

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IFAC Proceedings Volumes 2014年第3期47卷 10814-10819页

作者： Jianping Cai Changyun Wen Hongye Su Zhitao Liu The State Key Laboratory of Industrial Control Technology Institute of Cyber-Systems and Control Zhejiang University Hangzhou 310027 P.R.China School of Electrical and Electronic Engineering Nanyang Technological University Nanyang Avenue Singapore 639798

In this paper, we consider a class of second-order uncertain nonlinear systems. Besides parametric uncertainties, a non-parametric uncertainty may exist in every state equation or channel with its effects bounded by a known function. Such a bounding function is allowed to depend on all system states which means that the actual system does not need to meet the triangular structure. Therefore the currently available backstepping technique cannot be used to design controllers. To overcome such difficulty, a new backatepping-based robust adaptive control scheme is proposed. It is shown that the proposed scheme can ensure all signals in the closed-loop system bounded.

关键词： Backstepping Adaptive control Nonlinear system Uncertainties Non-triangular structure

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A green wave band based method for urban arterial signal control

A green wave band based method for urban arterial signal con...

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IEEE International Conference on Networking, Sensing and control

作者： Bao-Lin Ye Weimin Wu Xuanhao Zhou Weijie Mao Yi-Sheng Huang The State Key Laboratory of Industrial Control Technology & Institute of Cyber-Systems and Control Zhejiang University Hangzhou China Department of Electrical Engineering National Ilan University Taiwan China

Optimal signal timing is an efficient and effective method to mitigate traffic congestion in urban road traffic networks. In this paper, we propose a new method for signal-timing optimization of urban arterial roads. The main idea to the method is to design a bi-direction green wave band for arterial roads. In order to reduce delay and stops, an arterial road signal coordination approach is developed. In addition, the arterial signal coordination approach has been expanded to deal with the problem of coordination for urban traffic networks coordination control. Finally, simulation experiments are given to illustrate the effectiveness of the proposed method.

关键词： Artificial intelligence Vehicles Silicon

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An efficient mathematical model for a generalized production process

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AIP Conference Proceedings 2015年第1期1648卷

作者： Wei Weng Cheng Chen Gang Rong Shigeru Fujimura aGraduate School of Information Production and Systems Waseda University 2-7 Hibikino Wakamatsu-ku Kitakyushu Fukuoka 808-0135 Japan bState Key Laboratory of Industrial Control Technology Institute of Cyber-systems and Control Zhejiang University Hangzhou China 310027

This study proposes an efficient mixed integer linear programming (MILP) model for a generalized job shop production process. In the process, a workstation contains one or multiple machines and each job visits some of the workstations in a specific sequence. It is allowed that a job visits the same workstation more than once. Although some similar processes were modeled by MILP in previous studies, the models are unable to solve problems that involve more than ten jobs due to high computational complexity. Our proposed model outperforms the best model that is identified among 23 research papers with regard to computational complexity. Simulation results show that our model is able to solve a problem with a dozen of jobs, which is classified as a large-scale problem in the literature.

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Global asymptotic stability for a class of neural networks with time-varying delays

Global asymptotic stability for a class of neural networks w...

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2014中国制导、导航与控制学术会议

作者： Yingxin Guo Chao Xu School of Mathematical Sciences Qufu Normal UniversityQufuShandong 273165P.R.China State Key Laboratory of Industrial Control TechnologyInstitute of Cyber-Systems and ControlZhejiang UniversityHangzhouZhejiang310027P.R.China State Key Laboratory of Industrial Control Technology Institute of Cyber-Systems and ControlZhejiang UniversityHangzhouZhejiang310027P.R.China

ISBN: (纸本)9781479946983

The paper deals with the globally asymptotically stability of dynamical neural networks with time-varying *** sufficient conditions for the globally asymptotically stable of the neural networks are obtained by Lyapunov-Razumikhin ***,we discuss the stability conditions which do not require the activation functions to be differential,bounded,or monotone *** examples are also applied to illustrate the efficiency of the results.

关键词： Neural networks Lyapunov functionals Lyapunov-Razumikhin technique Global asymptotic stability

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Exponential Stabilization of an Unstable Parabolic PDE System Using Boundary Optimal control

Exponential Stabilization of an Unstable Parabolic PDE Syste...

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第三十三届中国控制会议

作者： Huachen Jiang Chao Xu Department of Mathematics Zhejiang University Lab of Information and Optimization Technology Ningbo Institute of Technology State Key Lab of Industrial Control Technology Institute of Cyber-Systems & Control Zhejiang University

ISBN: (纸本)9781479947249

In this paper, we consider an unstable linear parabolic diffusion-reaction PDE system with an actuator at the right boundary via the Dirichlet condition. We begin to derive the first order necessary conditions for the open loop finite-time horizon problem using the classical variational approach. Then, we discuss the the state-feedback problem, and obtain the feedback law by formulating the Riccati partial differential equation(PDE). Finally, with the feedback law, we could prove the stability for the state-feedback problem exponential stability with any given decay rate in the time domain without solving the Riccati PDE.

关键词： Boundary Actuation,Unstable PDE System,Open Loop control,Closed Loop Stability

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