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A relatively high zoonotic trematode prevalence in Orientogalba ollula and the developmental characteristics of isolated trematodes by experimental infection in the animal model

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Infectious Diseases of Poverty 2022年第4期11卷 72-80页

作者： Jian Li Yijing Ren Lei Yang Jiani Guo Haiying Chen Jiani Liu Haoqiang Tian Qingan Zhou Weiyi Huang Wei Hu Xinyu Feng Basic Medical College Guangxi Traditional Chinese Medical UniversityNanning 530005GuangxiChina College of Life Sciences Inner Mongolia UniversityHohhot 010070China College of Animal Science and Technology Guangxi UniversityNanning 530005GuangxiChina National Institute of Parasitic Diseases Chinese Center for Disease Control and PreventionKey Laboratory of Parasite and Vector Biology of China Ministry of HealthWHO Collaborating Centre for Tropical DiseasesJoint Research Laboratory of Genetics and Ecology On Parasite-Host InteractionChinese Center for Disease Control and PreventionFudan University200025 ShanghaiChina Department of Infectious Diseases Huashan HospitalState Key Laboratory of Genetic EngineeringMinistry of Education Key Laboratory for Biodiversity Science and Ecological EngineeringMinistry of Education Key Laboratory of Contemporary AnthropologySchool of Life SciencesFudan UniversityShanghai 200438China School of Global Health Chinese Center for Tropical Diseases ResearchShanghai Jiao Tong University School of MedicineShanghai 20025China One Health Center Shanghai Jiao Tong University-The University of EdinburghShanghai 20025China

Background:Food-borne parasitic diseases decrease food safety and threaten public *** snail species is an intermediate host for numerous human parasitic *** ollula has been reported as intermediate hosts of many zoonotic ***,we investigated the prevalence of zoonotic trematodes *** in Guangxi,China,and assessed their zoonotic ***:Snails were collected from 54 sites in 9 cities throughout *** snail and trematode larvae species were determined by combining morphological characteristics and molecular *** trematodes prevalence and constituent ratio were calculated and compared among different habitat *** trees of the trematode species were constructed using the neighbor-joining method with nuclear internal transcribed spacer 2(ITS2)*** developmental cycles of the isolated trematodes were examined by experimental infection in *** developmental characteristics ofEchinostoma revolutum was recorded by dissecting infected ducklings from 1-day post infection(dpi)to 10 ***:The overall prevalence of trematode larvae was 22.1%(1818/8238)*** from 11 sample *** together with molecular identification,showed ***,Australapatemon sp.,Hypoderaeum conoideum,Pharyngostomum cordatum,andEchinostoma ***,with the highest infection rate ***(13.0%).However,noFasciola larvae were *** trematodes prevalence and constituent ratio varied in two sub-biotypes(P<0.01).A neighbor-joining tree analysis of ITS2 sequences resulted in distinct monophyletic clades supported by sequences from isolated larvae with high bootstrap *** exposed *** infected withEchinostoma sp.,***,*** larvae were successfully *** animal model forEchinostoma revolutum was successfully *** matured from larvae to adult at 10 dpi in the intestine of the duck,and the devel

关键词： Orientogalba ollula Zoonotic trematode Intermediate host Prevalence ITS2

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Study on the Structure Regulation and Electrochemical Properties of Imidazole Based-Mofs by Small Molecules

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SSRN 2022年

作者： Wang, Xiao-Feng Zhao, Ji-Yang Jia, Meng-Qi Zhang, Xiu Du Xu, Xiao-Bing Cheng, Jiong-Jia Wang, Ying Liu, Guang-Xiang Chen, Kai College of Environmental Science Nanjing Xiaozhuang University Nanjing211171 China College of Chemistry and Materials Science Key Laboratory of Functional Molecular Solids Ministry of Education Anhui Laboratory of Molecule-Based Materials Anhui Key Laboratory of Functional Molecular Solids Anhui Normal University 189 Jiuhua Southern Road Wuhu241002 China Collaborative Innovation Center of Atmospheric Environment and Equipment Technology Jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control School of Environmental Science and Engineering Nanjing University of Information Science & Technology Nanjing210044 China

In this work, four novel metal-organic frameworks (MOFs) were synthesized by the solvothermal reaction of the transition metal salts Co(NO3)2with the mixed organic ligands 4,4’-sulfonyldibenzoic acid (H2SA) and 1,4-di(1H-imidazol-1-yl)-benzene (DIB) with the formula of [Co(DIB)0.5(SA)]•(DIB)0.5 (1), [Co(DIB)0.5(SA)] (2), [Co0.5(DIB)(HSA)]•(C10H8)0.5 (3) and [Co0.5(DIB)(HSA)]•(CH3CN) (4). Structural analysis manifest that complex 1 are assembled by the 2D Co-SA-DIB coordination networks constructed from the binuclear cobalt paddle-wheel secondary building units, while complex 3 are built from the 2D Co-DIB coordination networks based on the mononuclear cobalt center with the partially deprotonated HAS- ligand suspended on the two sides of the Co-DIB networks via the coordination between carboxylate and Co2+ cations. Furthermore, free DIB and naphthalene molecules function as the guest molecules to fill the pores of frameworks of 1 and 3 respectively. More impressively, by just changing the used solvent, the free guest molecules DIB and naphthalene could be removed from the pores and two MOFs, namely 2 and 4 with identical framework architecture to 1 and 3, respectively, could be obtained. In addition, the electrochemical properties of complexes 1-3 were checked as well and the results indicate that complex 3 exhibited the best electrocatalytic performance for the hydrogen evolution reaction (HER). © 2022, The Authors. All rights reserved.

关键词： Naphthalene

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Differentiated Strategies of Animal-Derived and Plant-Derived Biochar to Reduce Nitrogen Loss During Paper Mill Sludge Composting

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SSRN 2022年

作者： Liu, Xiaoming Wang, Yueqiang Zhou, Shaoqi Cui, Peng Wang, Weiwu Huang, Wenfeng Yu, Zhen Zhou, Shungui National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China Guangdong Key Laboratory of Integrated Agroenvironmental Pollution Control and Management Institute of Eco-environmental and Soil Science Guangdong Academy of Sciences Guangzhou510650 China College of Resources and Environmental Engineering Guizhou University Guizhou 550025 China Guangdong Provincial Engineering and Technology Research Center for Agricultural Land Pollution Prevention and Control Zhongkai University of Agriculture and Engineering Guangzhou510225 China Fujian Provincial Key Laboratory of Soil Environmental Health and Regulation College of Resources and Environment Fujian Agriculture and Forestry University Fuzhou350002 China

Biochar is widely used to increase nitrogen (N) retention during composting. However, the differences in the regulation of N cycling between animal-derived biochar and plant-derived biochar remain unclear. In this study, three treatments were established, including CK (no biochar), ABC (spiked animal-derived biochar), and PBC (spiked plant-derived biochar). Results showed that N loss was reduced by 24.4% and 35.5% in ABC and PBC, respectively, compared with CK. Moreover, the contents of acid-insoluble-N (AIN) in ABC and bioavailable organic N (BON) in PBC were higher than in CK. The protease activity and bacterial communities associated with organic N degradation increased in ABC, which contributed to the generation of humic N-containing precursors. Decreased urease activity and enhanced N-fixation were found in PBC. Structural equation model confirmed that BON retention and AIN formation were the dominant strategies for animal-derived and plant-derived biochar, respectively. The findings provided multiple pathways to produce N-enriched compost products. © 2022, The Authors. All rights reserved.

关键词： Animals

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Degree of Urbanization and Vegetation Type Shape Soil Biodiversity in City Parks

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SSRN 2023年

作者： Yao, Haifeng Li, Zhipeng Geisen, Stefan Qiao, Zhihong Breed, Martin F. Sun, Xin Key Laboratory of Urban Environment and Health Ningbo Observation and Research Station Fujian Key Laboratory of Watershed Ecology Institute of Urban Environment Chinese Academy of Sciences Xiamen361021 China University of Chinese Academy of Sciences Beijing100049 China Zhejiang Key Laboratory of Urban Environmental Processes and Pollution Control CAS Haixi Industrial Technology Innovation Center in Beilun Ningbo315830 China Key Laboratory of Wetland Ecology and Environment Northeast Institute of Geography and Agroecology Chinese Academy of Sciences Changchun130102 China Laboratory of Nematology Department of Plant Sciences Wageningen University & Research Wageningen6700 ES Netherlands College of Science and Engineering Flinders University Bedford ParkSA5042 Australia

Urbanization negatively impacts aboveground biodiversity, such as bird and insect communities. City parks can reduce these negative impacts by providing important habitat. However, it remains poorly understood how the degree of urbanization and vegetation types within city parks (e.g., lawns, woodland) impact soil biodiversity. Here we investigated the impact of the degree of urbanization (urban vs. suburban) and vegetation type (lawn, shrub-lawn, tree-lawn and tree-shrub mixtures) on soil biodiversity in parkland systems. We used eDNA metabarcoding to characterize soil biodiversity of bacteria, fungi, protists, nematodes, meso- and macrofauna across park vegetation types in urban and suburban regions in Xiamen, China. We observed a strong effect of the degree of urbanization on the richness of different soil biota groups, with higher species richness of protists and meso/macrofauna in urban compared to suburban areas, while the richness of bacteria and fungi did not differ, and the difference of nematode richness depended on vegetation type. At the functional level, increased degree of urbanization associated with greater species richness of bacterivores, plant pathogens and animal parasites. These urbanization effects were at least partly modulated by higher soil phosphorous levels in urban compared to suburban sites. Also, the vegetation type impacted soil biodiversity, particularly fungal richness, with the richness of pathogenic and saprotrophic fungi increasing from lawn to tree-shrub mixtures. Tree-shrub mixtures also had the highest connectedness between biotas and lowest variation in the soil community structure. Overall, we show that soil biodiversity is strongly linked to the degree of urbanization, with overall richness increasing with urbanization, especially in bacterivores, plant pathogens and animal parasites. Targeted management of vegetation types in urban areas should provide a useful way to help mitigate the negative effect of urbanization on soi

关键词： Bacteria

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Multi-Objective Optimization of Integrated Lithium-Ion Battery Thermal Management System

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SSRN 2022年

作者： Shi, Hong Liu, Meinan Li, Yonghao Wang, Shuheng Qiu, Chenghui Cheng, Mengmeng Yuan, Jie Yang, Kaijie Kang, Chuanzhi College of Energy & Power Engineering Jiangsu University of Science and Technology 2 Mengxi Jingkou Zhenjiang212003 China Key Laboratory of Aircraft Environment Control and Life Support MIIT Nanjing University of Aeronautics & Astronautics 29 Yudao Street Nanjing210016 China

The heat generation rate of lithium-ion battery increases dramatically with the increase in the charge/discharge rate of the battery. Battery thermal management system (BTMS) based on phase change materials (PCM) are demonstrating great performance in the field of high heat flux heat dissipation. However, traditional hybrid PCM modules focus too much on their thermal performance and ignore the weight issue, which greatly reduces their wide application. In this paper, a BTMS integrated air-cooling channels, fins and PCM is proposed to improve the thermal performance of the battery. Based on the entropy weight-TOPSIS method, the balance of battery cells temperature uniformity and lightweight of lithium batteries is studied and the parameters of the optimal BTMS are obtained. The results demonstrate that the thermal performance of the battery is enhanced as the increase of PCM volume, but the weight of the system also increases significantly. When the height of the fins increases, the reasonable utilization of PCM increases and the PCM melts more evenly. Entropy weight-TOPSIS can help us to find the optimal solution between thermal performance and lightweight design. After the TOPSIS algorithm search, the optimal structural solution corresponding to the PCM thickness ( d ) = 7.5 mm and the fin height ( h ) = 65 mm has the smallest Euclidean distance Si+ from the positive ideal solution, and the largest Euclidean distance Si- from the negative ideal solution, thus it obtains a higher Ci and the highest priority. The corresponding maximum temperature ( Tmax), temperature difference (∆T) and weight (W) are 319.29 K, 0.0135 K and 5.13 kg respectively. © 2022, The Authors. All rights reserved.

关键词： Multiobjective optimization

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MQP: Mutants Quality Prediction for Cost-Effective Mutation Testing

MQP: Mutants Quality Prediction for Cost-Effective Mutation ...

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IEEE International Conference on Software Quality, Reliability and Security Companion (QRS-C)

作者： Xingya Wang Shiyu Zhang Fangxiao Liu Lichao Feng Zhihong Zhao School of Computer Science and Technology Nanjing Tech University Nanjing China Command and Control Engineering College Army Engineering University of PLA Nanjing China State Key Laboratory for Novel Software Technology Nanjing University Nanjing China

ISBN: (纸本)9781665478373

Mutation testing simulates typical software defects by executing mutation operations. It can effectively measure the defect detection capabilities of a test-suite. However, in the mutation testing, we must generate, compile, and execute plenty of mutants, which causes substantial computing costs. Mutants selection can reduce the number of mutants but cannot avoid compiling and executing all mutants. Therefore, mutation testing suffers an efficiency problem, making it hard to use in practice. In this paper, we propose a Mutants Quality Prediction method, MQP, which relies on the static features of the source program, test-suite, and mutants to predict the quality of a test-suite. MQP avoids compiling and executing all mutants during mutants selection and thus can save expensive computing costs. Then, we use MQP as the fitness function and select the evolutionary optimization strategy, Genetic Algorithm, to generate a high-quality mutant subset. We empirically studied MQP on nine widely used items. The experimental results show that MQP achieves a good predictive effect. Its accuracy rate can reach 98.30%, while the average absolute error is only 0.74%. Moreover, the reduction efficiency of the MQP-based mutation testing is about 20 times that of the random-based selective mutation testing.

关键词： Software testing Costs Static analysis Software quality Predictive models Software reliability Security

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Identification of the Groundwater Hydrodynamic and Hydrochemical Spatiotemporal Coupling Response of Multi-Aquifer Systems Under the Disturbance of Deep and Special Thick Coal Seam Mining

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SSRN 2023年

作者： Zhan, Hao Liu, Shouqiang Wu, Qiang Liu, Weitao Shi, Lihu Liu, Dong Gao, Shuai National Engineering Research Center of Coal Mine Water Hazard Controlling China University of Mining and Technology Beijing100083 China College of Geoscience and Surveying Engineering China University of Mining and Technology Beijing100083 China Key Laboratory of Mine Water Control and Resource Utilization State Administration of Mine Safety Supervision Beijing100083 China Inner Mongolia Research Institute Inner Mongolia Ordos017000 China Shandong University of Science and Technology China CNACG Ecological Environment Technology Co. Ltd. Beijing100067 China

The groundwater hydrodynamic and hydrochemical process of the multi-aquifer system will experience complicated and serious influence under deep coal mining disturbance. The study of the groundwater hydrodynamic and hydrochemical spatiotemporal coupling response of multi-aquifer systems under the disturbance of deep and special thick coal seam mining in ecologically fragile western mining areas is of great significance for the safe mining of coal resources and ecological environment protection. In this study, the hydrochemical analysis data composed of 218 groundwater samples from Tangjiahui coalfield, Northwest China with 1526 measurements and a 7-year (2016-2021) sampling period were collected for studying the hydrogeochemical spatiotemporal evolution process and control mechanism of the multi-aquifer system using hierarchical cluster analysis, ion-ratio method, saturation index and multidimensional statistical analysis. In addition, wavelet analysis and cross-wavelet coherence analysis were applied to quantitatively recognize the spatiotemporal variation characteristics of hydrodynamic information and analyze the coherence relationships between time series. The results demonstrate that the hydrochemical characteristics exhibit significant spatial differences, while the temporal variation of hydrochemical characteristics in the Permian Shanxi Formation fractured sandstone aquifer (PSFFA), mine water (MW), and Ordovician karst limestone aquifer (OKA) is not significant. The water-rock interaction is the predominant control mechanism for the spatial evolution of hydrogeochemistry in the study area. Moreover, the large-scale mining of deep coal seams controls the type and degree of water-rock interactions by damaging the structure of aquifers and altering the hydrodynamic conditions of groundwater. The period from 2016 to 2021 exhibits multi-time scale characteristics in time series of precipitation, mine water inrush, and the water level of PSFFA and OKA. The mine wa

关键词： Time series

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The continuous increased stability of sediment dissolved organic matter implies ecosystem degradation of lakes in the cold and arid regions

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science of the Total Environment 2024年 947卷 174384页

作者： Guo, Ying Liu, XiaoFei Dong, Yue Ni, Zhaokui Zhou, Chunyang Chen, Chao Wang, Shengrui Chen, Qiuying Yan, Yanting Guangdong-Hong Kong Joint Laboratory for Water Security Center for Water Research Advanced Institute of Natural Sciences Beijing Normal University at Zhuhai Zhuhai519087 China College of Water Sciences Beijing Normal University Beijing100875 China Engineering Research Center of Ministry of Education on Groundwater Pollution Control and Remediation Beijing100875 China Instrumentation and Service Center for Science and Technology Beijing Normal University at Zhuhai Zhuhai519087 China Yunnan Key Laboratory of Pollution Process and Management of Plateau Lake Watershed Yunnan Province Kunming650034 China North China Institute of Science and Technology School of Chemical Safety Langfang101601 China

The characteristics of lake dissolved organic matter (DOM) pool and lake ecosystem interact, and studying the responses between sediment DOM characteristics and lake ecosystem changes may shed light on the inherent connection between ecosystem evolution and carbon biogeochemical cycles. Lakes in cold and arid regions are sensitive to changes and accumulate large amounts of carbon as DOM, which may provide a window into more explicit relationships between ecosystem evolution and changes in sediment DOM characteristics in time dimension. However, considerable blind spots exist in the responses between the sediment DOM and ecosystem evolution on time scale and the underlying mechanisms. In this study, multiple approaches were combined to investigate the relationship between the variation trend of sediment DOM characteristics and the evolution of fragile lake ecosystems across three different lake ecosystems in cold and arid regions of China. A strong positive relationship between sediment DOM stabilities, especially humification, and ecosystem degradation was found, consistent for the three lakes. Ultra-high-resolution mass spectrometry and structural equation modeling revealed that the changes of ecosystems affected sediment DOM stability through direct pathways (0.24), such as the contents of terrestrial DOM in lake DOM pool, and indirect pathways, including algae-mediated (0.43) and salinity-mediated pathways (0.22), which all increased the contents of refractory DOM in the lake DOM pool and sediments. Based on the fact that DOM stability changes could act on the ecosystem in turn, a possible positive feedback mechanism between ecosystem degradation and increased DOM stability was further inferred. These results suggested that the continuous increased stability of sediment DOM in may implies ecosystem degradation of lakes in the cold and arid regions. This study provides a new perspective for recognizing ecosystem evolution through sediment DOM and improves the unde

关键词： Lakes

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Mil-101(Fe)@Zn/Co-Zifs Metal–Organic Frameworks are Effective Photo-Fenton Catalysts for Rhb Removal

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SSRN 2022年

作者： Li, Yucheng Wang, Xiangyu Duan, Zhengyin Yu, Dehai Wang, Qiang Ji, Dandan Liu, Wenxia State Key Laboratory of Biobased Material and Green Papermaking Qilu University of Technology Shandong Academy of Sciences Jinan250353 China Guangxi Key Laboratory of Clean Pulp & Papermaking and Pollution Control College of Light Industry and Food Engineering Guangxi University Nanning530004 China School of Environmental Science and Engineering Qilu University of Technology Shandong Academy of Sciences Jinan250353 China National Forestry and Grassland Administration Key Laboratory of Plant Fiber Functional Materials Fujian Fuzhou350108 China

Treating dye-contaminated wastewater using the visible light–driven photo-Fenton reaction is beneficial to environmental remediation. This paper reports the synthesis of a novel, highly effective photo-Fenton catalyst, the MIL-101(Fe)@Zn/Co-ZIFs composite, by a facile internal-growth method. The composite, which had an MOF-in-MOF structure, activated H2O2 to decompose RhB, a polluting-dye model, under visible-light illumination. Via the Z-scheme, the transfer of the electron–hole pair was synergistically accelerated by the reaction centers of the composite (the MIL-101(Fe) shell and Zn/Co-ZIFs core), which enhanced H2O2 activation. The generated •OH, •O2−, and 1O2 effectively decomposed RhB. The composite’s catalytic performance was significantly enhanced by the electronic hybridization of its Fe3+, Co2+, and Zn2+ metal centers. Thus, the composite demonstrated a remarkable capability to remove RhB. This study provides a novel method to construct an MIL-101(Fe)@Zn/Co-ZIFs catalyst with dual reaction centers that enhance the transfer of electron–hole pairs. Our findings inspire the application of MOF-based photo-Fenton catalysts to wastewater treatment and environmental remediation. © 2022, The Authors. All rights reserved.

关键词： Catalysts

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Ultra-fast detoxification pathways of anti-cancer drug (Gefitinib) via high-entropy engineering driving catalysis

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Water Research 2025年 284卷 123957页

作者： Bai, Yuhe Tao, Qiangqiang Wang, Li Lu, Shuai Zhang, Aixin Li, Kang Li, Xuan He, Kuang Hu, Ning College of Resources and Environmental Engineering Wuhan University of Science and Technology Hubei Wuhan 430081 China Key Laboratory of Ultra-fast Catalytic Reaction of Hubei Provincial Engineering Research Center for Organic Wastewater Treatment Hubei Wuhan 430081 China Center of Green Control and Remediation Technologies of Environmental Pollution Hubei Wuhan 430081 China South China Institute of Environmental Sciences Guangdong Province Guangzhou 510655 China

Gefitinib (GEF), a commonly detected anticancer drug in medical wastewater, also serves as a persistent organic pollutant in aquatic ecosystems. The complex chemical structure of GEF renders it resistant to microbial and enzymatic degradation. A high-entropy oxide catalyst (FeCoNiMnCu)Ox/13X (FCNMC/13X) was synthesized to generate reactive oxygen species (ROS) for GEF degradation. At 120 °C, FCNMC/13X achieved a 99.48 % GEF degradation rate within 120 s. At ambient temperature (35 °C), it reached 94 % degradation within 30 min. The catalytic degradation activity is primarily driven by ROS, with ·OH as the dominant species. The degradation pathway of GEF and the biotoxicity of its intermediate products were proposed. Finally, density functional theory (DFT) calculations revealed the presence of free electrons in the high-entropy catalyst, demonstrating exceptional electron transfer properties that enhance adsorption and dissociation on the catalyst surface. The catalyst exhibits high efficiency in environmental remediation at elevated temperatures and effective drug detoxification at ambient temperature, underscoring its broad applicability in both environmental and biomedical applications. © 2025 Elsevier Ltd

关键词： Anti-cancer drugs Density functional theory (DFT) Detoxification High entropy Ultra-fast catalytic

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