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ACS Electrochemistry 2024年第1期1卷 63-72页

作者： Shenglin Liu Menglin Wu Chaozhong Wang Yihang Li Tong Liu Jiupai Ni John Irvine Chengsheng Ni College of Resources and Environment Southwest University Chongqing 400715 China；Key Laboratory of Energy Thermal Conversion and Control of Ministry of Education School of Energy and Environment Southeast University Nanjing 211189 Jiangsu China College of Resources and Environment Southwest University Chongqing 400715 China；Institute of Energy Hefei Comprehensive National Science Center Hefei Anhui 230031 P. R. China College of Resources and Environment Southwest University Chongqing 400715 China Interdisciplinary Research Center of Smart Sensors Academy of Advanced Interdisciplinary Research Xidian University Xi’an 710071 P. R. China Key Laboratory of Green Chemical Process of Ministry of Education Key Laboratory of Novel Reactor and Green Chemical Technology of Hubei Province School of Chemical Engineering and Pharmacy Wuhan Institute of Technology Wuhan 430205 China College of Resources and Environment Southwest University Chongqing 400715 China；School of Chemistry University of St Andrews St Andrews KY16 9ST United Kingdom

Sr or Ca doping on the Ba site of layered perovskites is reported to increase oxygen reduction reaction catalytic activity, but the underlying mechanism of this phenomenon for the protonic cell is not clear. In this research, Ca containing La0.5(Ba0.5–xCax)FeO3-δ(0≤x≤0.3) perovskites are explored as cathodes for proton-conducting direct ammonia fuel cells. La0.5Ba0.5FeO3-δ(LBF) has mixed cubic and tetragonal phases with cation ordering, and the Ca doping generally increases the oxide-ion conductivity because it could increase the stability of random cation distribution on the A site and facilitate the formation of oxide ion vacancy in the layer according to the density-function theory (DFT) calculation. The surface exchange coefficient (2.00 × 10–5cm s–1) and the chemical diffusion coefficient (4.01 × 10-10cm2s–1) are enhanced to 8.56 × 10–5cm s–1and 7.37 × 10–9cm2s–1, respectively, when the Ca content increased from 0 to 30%. For electrochemical tests, the single cell with a cathode of cubic La0.5(Ba0.45Ca0.05)FeO3−δbased on BaZr0.1Ce0.7Y0.2O3(BZCY) electrolyte shows a polarization resistance of 0.058 Ω cm2at 700 °C, imparting a peak power density of 682 mW cm–2under NH3fuel. The Ca2+doping abovex= 0.05 would decrease the cell output or stability for continuous operation in fuel-cell mode because of the decreased protonic conductivity that could localize the site for water production. This research highlights the effect of isovalent Ca2+doping on the Ba2+site and demonstrates the possibility for a proton conducting cathode for direct ammonia fuel cell.

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Effects of Deletion of Siderophore Biosynthase Gene in Pseudomonas fragi on Quorum Sensing and Spoliage Ability

SSRN

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SSRN 2022年

作者： Cui, Fangchao Wang, Qianqian Tan, Xiqian Wang, Dangfeng Li, Jianrong Li, Tingting Liu, Jiayi College of Food Science and Technology Bohai University National & Local Joint Engineering Research Center of Storage Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products Liaoning Jinzhou121013 China Key Laboratory of Biotechnology and Bioresources Utilization Dalian Minzu University Ministry of Education Liaoning Dalian116029 China College of Food Science and Technology Jiangnan University Jiangsu Wuxi214122 China

Siderophore is an important spoilage factor in the spoilage process of Pseudomonas fragi, the dominant spoilage bacterium of aquatic products, and its secretion is involved in the quorum sensing (QS). In this study, P. fragi (MS-10) which separated from spoilage tuna were used as test strain. Then, a mutant strain with deletion of this siderophore biosynthesis gene was constructed and explored the effects of its on growth tolerance, QS and spoilage potential of P. fragi. Results showed that the deletion of the siderophores biosynthesis gene slowed the growth rate of the strain. The apoptosis rate increased by 27.7% compared to the wild strain at 4 °C for 48 h. Biofilm formation, extracellular protease expression, and signal molecule production were all significantly lower in the mutant strain than in the wild strain. According the total viable count and histamine showed that the tuna sterile fish block inoculated with the wild strain exceeded acceptable standards by 5 days and were completely spoiled by 7 days, whereas the mutant strain exceeded acceptable standards by 6 days and were completely spoiled by 9 days. The pH, texture and other indicators showed that the variation range of the mutant strain group was smaller than that of wild strain group. The deletion of the siderophores biosynthesis gene reduced the spoilage ability of P. fragi. Based on the results, the development of novel preservation agents targeting the control of siderophores biosynthesis gene could be a new idea for the preservation of aquatic products. © 2022, The Authors. All rights reserved.

关键词： Bacteria

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Chromosome-level genomes of seeded and seedless date plum based on third-generation DNA sequencing and Hi-C analysis

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Forestry Research 2021年第1期1卷 77-85页

作者： Weitao Mao Guoxin Yao Shangde Wang Lei Zhou Guosong Chen Ningguang Dong Guanglong Hu Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China) Ministry of AgricultureBeijing Engineering Research Center for Deciduous Fruit TreesBeijing Academy of Forestry and Pomology SciencesBeijing 100093China Hubei Key Laboratory of Quality Control of Characteristic Fruits and Vegetables College of Life Science and TechnologyHubei Engineering UniversityXiaogan 432000China School of Life Science Hubei UniversityWuhan 430062China Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement Food Crops InstituteHubei Academy of Agricultural SciencesWuhan 430072China Beijing XinTaoYuan Commerce&Trading Co. Ltd.Beijing 101215China

Diospyros lotus L.(Date plum)is an important tree species that produces fruit with a high nutritional *** accurate chromosomal assembly of a species facilitates research on chromosomal evolution and functional gene *** this study,we assembled the first chromosome-level genomes of seeded and seedless *** using Illumina short reads,PacBio long reads,and Hi-C *** assembled genomes comprising 15 chromosomes were 617.66 and 647.31 Mb in size,with a scaffold N50 of 40.72 and 42.67 Mb for the seedless and seeded ***,respectively.A BUSCO analysis revealed that the seedless and seeded *** genomes were 91.53% and 91.60%complete,***,20,689(95.4%)and 22,844(98.5%)protein-coding genes in the seedless and seeded *** genomes were annotated,*** of the chromosomes between genomes revealed inversions and translocations on chromosome 8 and inversions on chromosome *** identified 490 and 424 gene families that expanded in the seedless and seeded ***,*** enriched pathways among these gene families included the estrogen signaling pathway,the MAPK signaling pathway,and biosynthetic pathways for flavonoids,monoterpenoids,and ***,we constructed the first Diospyros genome database(http://***).On the basis of our data,we developed the first high-quality annotated *** reference genomes,which will be useful for genomic studies on persimmon and for clarifying the molecular mechanisms underlying important *** between the seeded and seedless *** genomes may also elucidate the molecular basis of seedlessness.

关键词： BUS database analysis

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Functional Magnetic Nanoparticles Combined with Molecular Dynamics technology to Screen Quorum Sensing Inhibitors from Natural Substances: Accuracy, Efficiency and High Throughput

SSRN

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SSRN 2022年

作者： Cui, Fangchao Xi, Liqing Wang, Dangfeng Tan, Xiqian Li, Jianrong Li, Tingting College of Food Science and Technology Bohai University National & Local Joint Engineering Research Center of Storage Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products Liaoning Jinzhou121013 China Key Laboratory of Biotechnology and Bioresources Utilization Dalian Minzu University Ministry of Education Liaoning Dalian116029 China College of Food Science and Technology Jiangnan University Jiangsu Wuxi214122 China

Effective quorum sensing inhibitor (QSI) is an important way to solve microbial pollution. The traditional QSI screening method is time-consuming and low accuracy. In order to solve these problems, a more efficient and accurate method, ligand fishing bound HPLC-MS coupled molecular docking technology, was developed. In this study，Fe3O4@PS-ANTA-Ni2+ magnetic nanoparticles (MNPs) were synthesized in the first stage, with 97.4% capture of the HalR (His-Tag) protein. Subsequently, MNPs with fixed protein was used to fish for the active substances in laurel extract. After the active substances were identified by HPLC-MS, QSI was accurately located by molecular docking. Phytol was selected as a potential QSI and put into subsequent validation experiments. The results showed that the sub inhibitory concentration was 0.6 μL/mL, it has better inhibitory effect on swarming, extracellular protease, and violacein capacity of Hafnia alvei;among them, 51.1% and 51.8% inhibition of swimming and biofilm capacity, respectively. In summary, the method established in this study has the advantages of high efficiency, accuracy and high throughput, which realizes the screening of effective QSI from complex natural extracts. To a certain extent, it solves the problems caused by microbial pollution. © 2022, The Authors. All rights reserved.

关键词： Efficiency

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Robust Inertial-aided Underwater Localization based on Imaging Sonar keyframes

arXiv

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arXiv 2021年

作者： Xu, Yang Zheng, Ronghao Zhang, Senlin Liu, Meiqin The College of Electrical Engineering Zhejiang University Hangzhou310027 China State Key Laboratory of Industrial Control Technology Zhejiang University Hangzhou310027 China The Institute of Artificial Intelligence and Robotics Xi'an Jiaotong University Xi’an710049 China

This article focuses on the feature-based underwater localization and navigation for autonomous underwater vehicles (AUVs) using 2D imaging sonar measurements. The sparsity of underwater acoustic features and the loss of elevation angle in sonar images may introduce wrong feature matches or insufficient features for optimization-based underwater localization (i.e. under-constrained/degeneracy cases). This motivates us to propose a novel inertial-aided sliding window optimization framework to improve the estimation accuracy and the robustness to front-end outliers. Concretely, we first discriminate under-constrained/well-constrained sonar frames and define sonar keyframes (SKFs) based on the Jacobian matrix derived from odometry and sonar measurements. To utilize the past well-constrained SKFs mostly, we design a size-adjustable windowed back-end optimization scheme based on singular values. We also prove that the landmark triangulation failure (navigation problem) caused by sonar motion can be solved in 2D scenes. Comparative simulation and evaluation on a public dataset show the proposed method outperforms the existing ones in pose estimation and robustness even without loop closure, and also ensure the real-time performance for online applications. Copyright © 2021, The Authors. All rights reserved.

关键词： Sonar

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Real‐time superresolution interferometric measurement enabled by structured nonlinear optics

arXiv

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arXiv 2022年

作者： Zhang, Xin-Yu Wu, Hai-Jun Yu, Bing-Shi Rosales-Guzmán, Carmelo Zhu, Zhi-Han Hu, Xiao-Peng Shi, Bao-Sen Zhu, Shi-Ning Wang Da‐Heng Center HLJ Key Laboratory of Quantum Control Harbin University of Science and Technology Harbin150080 China National Laboratory of Solid‐State Microstructures College of Engineering and Applied Sciences Nanjing University Nanjing210093 China Centro de Investigaciones en Óptica A.C. Loma del Bosque 115 Colonia Lomas del Campestre Gto. León37150 Mexico CAS Key Laboratory of Quantum Information University of Science and Technology of China Hefei230026 China

Optical interferometers are pillars of modern precision metrology, but their resolution is limited by the wavelength of the light source, which cannot be infinitely reduced. Magically, this limitation can be circumvented by using an entangled multiphoton source because interference produced by an N-photon amplitude features a reduced de Broglie wavelength λ/N. However, the extremely low efficiency in multiphoton state generation and coincidence counts actually negates the potential of using multiphoton states in practical measurements. Here, we demonstrate a novel interferometric technique based on structured nonlinear optics, i.e., parametric upconversion of a structured beam, capable of superresolution measurement in real time. The main principle relies in that the orbital angular momentum (OAM) state and associated intramodal phase within the structured beam are both continuously multiplied in cascading upconversion to mimic the superresolved phase evolution of a multiphoton amplitude. Owing to the use of bright sensing beams and OAM mode projection, up to a 12-photon de Broglie wavelength with almost perfect visibility is observed in real time and, importantly, by using only a low-cost detector. Our results open the door to real-time superresolution interferometric metrology and provide a promising way toward multiphoton superiority in practical applications. © 2022, CC BY.

关键词： Nonlinear optics

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An activatable aptamer probe Q2c for detection of grouper iridovirus

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Aquaculture 2025年 609卷

作者： Liu, Mingzhu Huang, Jing Huang, Lin Qin, Qiwei Chang, Yanlei Shi, Lei Sun, Yun Zhou, Yongcan Wang, Shaowen Han, Shuyu Yu, Qing Li, Pengfei Guangxi Academy of Marine Sciences Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture Guangxi Engineering Research Center for Fishery Major Diseases Control and Efffcient Healthy Breeding Industrial Technology (GERCFT) Guangxi Academy of Sciences Nanning China College of Marine Sciences South China Agricultural University China Guangzhou Double Helix Gene Technology Co. Ltd Guangzhou China Sanya Institute of Breeding and Multiplication School of Marine Biology and Fisheries Collaborative Innovation Center of Marine Science and Technology Hainan University China Guangxi Fisheries Technology Extension Station Nanning China

Singapore grouper iridovirus (SGIV) severely impacts the health of marine fish species such as grouper, leading to high rates of mortality and significant economic losses. Effective monitoring, prevention, and nutritional management are crucial for controlling the disease. In recent years, activatable aptamers have emerged as promising biological probes, demonstrating great potential in the specific recognition and detection of viruses. In this study, the application and advantages of a target-activatable aptamer probe (TAA-Q2c2) in the identification of SGIV were investigated. Firstly, the specificity of TAA-Q2c2 was established for SGIV. Then, the optimal experimental conditions were determined to be a working concentration of 500 nM, an incubation temperature of 4–28 °C, and an incubation time of 1 min. The detection sensitivity was comparable to that of RT-qPCR at approximately 10³ SGIV-infected cells/mL;however, the activatable probe is more suitable for aquaculture field applications, and detection can be completed in less than two hours. In conclusion, TAA-Q2c2 enables the rapid and specific detection of SGIV, and is a promising new tool for the early detection of virus infection in aquaculture. © 2024

关键词： Activatable probe Aptamer Grouper iris virus Rapid detection

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Unveiling the Unexpected C-C Dissociation Capacity and Intrinsic Mechanism of Undercoordinated Pd Cluster/Ceo2 for the Efficient and Stable Catalytic Mineralization of Propane

SSRN

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SSRN 2022年

作者： Xia, Lianghui Jian, Yanfei Wang, Jingjing Wang, Yao Chai, Shouning Liu, Yujie Li, Lu Yu, Yanke Albilali, Reem He, Chi State Key Laboratory of Multiphase Flow in Power Engineering School of Energy and Power Engineering Xi'an Jiaotong University Shaanxi Xi’an710049 China Department of Chemistry College of Science Imam Abdulrahman Bin Faisal University P.O. Box 1982 Dammam31441 Saudi Arabia National Engineering Laboratory for VOCs Pollution Control Material & Technology University of Chinese Academy of Sciences Beijing101408 China

Light alkanes extensively presented in industrial exhausts have led tremendous harm to the atmospheric environment and human health. However, their stable molecule structure and inferior adsorption and activation efficiency make them as one category of the most intractable pollutants to be efficient controlled. Herein, we developed a Pd single cluster/CeO2 catalyst (Pd/CeO2-SCC-R) by a scalable deposition precipitation strategy, which demonstrates unexpected activity (propane total destructed below 300 oC) and thermal stability (even dramatically treated at 800 oC for multiple cycles) attributed to the strong metal-support interaction (SMSI) and unsaturated Pd metal sites caused by the shorter Pd-O distance. Mechanism studies determine that the Pd/CeO2-SCC-R possesses highly-efficient C-C cleavage ability owing to the persistent formation of large amounts of oxygen vacancies and low-coordinated Pd species, and the addition of H2O intrinsically accelerates propane activation and intermediate mineralization attributed to suppression of by-products by OH species. In comparison, the Pd single atom/CeO2 catalyst is preferentially for C-H bonds cleavage and inactive for C-C bonds cracking due to the saturated Pd sites anchored to the oxygen of supports directly, which generates more non-oxidation by-products as propyne and propylene, inhibiting the continuous decomposition of propane due to the retardatory oxidation of pyruvate species. The present work sheds critical insight to the production of fulfilling products through the directional cleavage of C-C/C-H bonds in the activation of light alkane molecules. © 2022, The Authors. All rights reserved.

关键词： Oxygen vacancies

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Screening of Xanthine Oxidase Inhibitory Peptides by Ligand Fishing Combined with Molecular Docking technology: High-Value Utilization of By-Products from Scophthalmus maximus

SSRN

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SSRN 2022年

作者： Cui, Fangchao Xi, Liqing Tan, Xiqian Wang, Dangfeng Li, Jianrong Zhao, Guiqin Li, Tingting College of Food Science and Technology Bohai University National & Local Joint Engineering Research Center of Storage Processing and Safety Control Technology for Fresh Agricultural and Aquatic Products Liaoning Jinzhou121013 China Key Laboratory of Biotechnology and Bioresources Utilization Dalian Minzu University Ministry of Education Liaoning Dalian116029 China College of Food Science and Technology Jiangnan University Jiangsu Wuxi214122 China

Gout, as the second major metabolic disease after cardiovascular disease. Peptides have the advantages of natural and no side effects in the treatment and prevention of gout. In this paper, xanthine oxidase (XOD) inhibitory peptides were screened from Scophthalmus maximus protein by ligand fishing technique. The specific results are as follows: Immobilization of xanthine oxidase 25.564 μg/mg by modified magnetic beads, and can be reused four times. Three decapeptides (FSLVHYAGTV, FTNEKLQQFF and WDDMEKIWHH) were screened, and the IC50 were 3.741, 4.046, 2.203 mg/mL. Circular dichroism and three-dimensional fluorescence spectra showed that the inhibitory peptide would change the helical structure of XOD and the internal hydrophobic environment. Molecular docking showed that the inhibitory effect of inhibitory peptides depends on the number of special amino acid residues in the active center of XOD enzyme. In conclusion, this study provides a method for screening anti gout peptides, which not only solve the problem of treating gout, but also realize the high-value utilization of marine products, with the effect of killing two birds with one stone. In addition, this method has strong application value because of its low cost and simple operation. © 2022, The Authors. All rights reserved.

关键词： Peptides

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A privacy preserving distributed controller for the general formation of multi-agent systems in port-Hamiltonian form

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Automatica 2025年 179卷

作者： Jingyi Zhao Yongxin Wu Yuhu Wu Yann Le Gorrec The Key Laboratory of Intelligent Control and Optimization for Industrial Equipment of Ministry of Education and the School of Control Science and Engineering Dalian University of Technology Dalian 116024 China Université Marie et Louis Pasteur SUPMICROTECH CNRS institut FEMTO-ST F-25000 Besançon France

This paper considers the general formation control problem under the port-Hamiltonian framework and proposes a method to meet the requirements of general formation control while protecting agent privacy. First, the general formation control problem is expressed as an optimization problem whose solution satisfies the requirements of the general formation. To protect the sensitive data of each agent, a distributed controller is designed through the desired general formation output dynamic, which still maintains a port-Hamiltonian form, making the Hamiltonian function the natural choice for the Lyapunov function candidate. It is then shown that the designed system converges exponentially to the global optimum of the optimization problem. Finally, simulations on an application case, namely underactuated unmanned surface vehicles with different parameters are provided to verify the effectiveness of the proposed method.

关键词： Distributed control General formation control Multi-agent systems Port-Hamiltonian systems Privacy preserving

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