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Gold Nanoparticles Based Colorimetric Detection of Target DNA After Loop-mediated Isothermal Amplification

Chemical Research in Chinese Universities 2013年第3期29卷 424-428页

作者： ZHOU Chao MU Ying YANG Meng-chao WU Qing-qing XU Wei ZHANG Ying JIN Wei SONG Qi WU Zhong-yu JIN Qin-han Research Center for Analytical Instrumentation Institute of Cyber-Systems and Control State Key Laboratory of Industrial Control Technology Zhejiang University Hangzhou 310058 P. R. China Central Laboratory Affiliated Hospital of Guiyang Medieal College Guiyang 550004 P. R. China School of Mathematics and Science Shijiazhuang University of Economics Shijiazhuang 050031 P. R. China

We have developed a rapid, simple and label-free colorimetric method for the identification of target DNA It is based on loop-mediated isothermal amplification（LAMP）. Plain gold nanoparticles（AuNPs） are used to indicate the occurrence of LAME The amplified product is mixed with AuNPs in an optimized ratio, at which the deoxyribonucleotides（dNTPs） bind to the AuNPs via ligand-metal interactions and thus enhance AuNPs stability. If a target DNA is amplified, the dramatic reduction of the dNTPs leads to the aggregation of AuNPs and a color change from red to blue. The success of the method strongly depends on the ionic strength of the solution and the initial concentration of dNTPs. Unlike other methods for the identification of isothermal products, this method is simple and can be readily applied on site where instrumentation is inadequate or even lacking.

关键词： Label-free Gold nanoparticle Loop-mediated isothermal amplification Naked eyes

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Index Modulated OFDM with Interleaved Grouping for V2X Communications

Index Modulated OFDM with Interleaved Grouping for V2X Commu...

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17th International IEEE Conference on Intelligent Transportation systems & The Asia-Pacific Council on systems Engineering Conference 2014

作者： Xiang Cheng Miaowen Wen Liuqing Yang Yuke Li School of Electronics Engineering and Computer Science Peking University National Mobile Communications Research Laboratory Southeast University School of Electronic and Information Engineering South China University of Technology State Key Laboratory of Management and Control for Complex Systems Institute of AutomationChinese Academy of Sciences

The rapid penetration of intelligent transportation systems（ITS） into the conventional transportation infrastructure urgently calls for high spectral efficiency high reliability communication technology for vehicle-to-vehicle and vehicle-to-infrastructure（V2X） *** orthogonal frequency division multiplexing（OFDM） is widely considered as a promising candidate for such applications,in this paper we propose a novel variation of OFDM for improved spectral efficiency as well as enhanced reliability in V2 X channels with correlated frequency-selective fading and inevitable Doppler *** proposed scheme is built upon a recently emerging technique termed as index modulated（IM-）***,different from the existing localized subcarrier grouping,we propose interleaved subcarrier *** then carry out analytical and simulated comparisons to demonstrate the merits of this new scheme in terms of both the bit error rate（BER）performance and the maximum achievable rate（MAR） of the overall system,in V2 X channels.

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Determination of soil nitrogen and organic carbon from near-infrared reflectance with variable selection

Determination of soil nitrogen and organic carbon from near-...

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American Society of Agricultural and Biological Engineers Annual International Meeting 2013

作者： Jia, Shengyao Yang, Xianglong Zhang, Jianming Li, Guang School of Biosystems Engineering and Food Science Zhejiang University Hangzhou 310058 China Key Laboratory of Equipment and Informatization in Environment Controlled Agriculture Ministry of Agriculture Hangzhou 310058 China State Key Laboratory of Industrial Control Technology Institute of Cyber-Systems and Control Zhejiang University Hangzhou 310027 China

ISBN: (纸本)9781627486651

The aims of this work were: to select informative variables for modeling near-infrared spectra to soil nitrogen (N) and organic carbon (OC) and to provide interpretation for the selected variables. The dataset that consisted of 225 soil samples was spilt into calibration set, validation set and prediction set by Knennard-Stone algorithm. Spectra in calibration set were conducted for variable selection by the method of Monte Carlo uninformative variable elimination (MC-UVE) and successive projections algorithm (SPA). Partial least squares regression (PLSR) and multiple linear regression (MLR) were used to construct calibration models for each property based on the selected variables. The proposed method MC-UVE-PLSR achieved the optimal performance for soil N and OC comparing with full-spectrum PLSR and SPA-MLR. The coefficients of determination (R2), residual prediction deviation (RPD) were respectively 0.86, 0.87, and 2.8, 2.7 for N and OC. The results indicate that MC-UVE is an effective tool for spectral variable selection, and is able to promote model prediction accuracy and efficiency. Analysis of the feature variables show that some of the selected variables of soil N and OC are directly related to their functional groups, while some influence the results by measuring the correlation constituents of N and OC. It is also suggested to adopt its own spectral response for the prediction of soil N instead of calculation by the autocorrelation of soil OC.

关键词： Organic carbon

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Biped walking on level ground with torso using only one actuator

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Science China(Information Sciences) 2013年第11期56卷 256-264页

作者： FENG Shuai Al Yahmadi Amur S. SUN ZengQi State Key Laboratory of Intelligent Technology and Systems Tsinghua National Laboratory for Information Science and Technology Department of Computer Science and TechnologyTsinghua University Beijing Institute of Control Engineering Department of Mechanical and Industrial Engineering College of Engineering Sultan Qaboos UniversityAl-Khod 123 P.O. Box 33Sultanate of Oman

It is well known that a biped robot needs actuators to walk stably on level *** now,a biped robot with torso has needed at least two actuators to achieve *** it be possible for this kind of robot to walk on level ground with only one actuator?This paper responds in the afrmative and proposes a simple control strategy for a planar biped robot with *** this control method,there is only one low gain proportional-derivative(PD)controller between the torso and the stance leg,while the swing leg remains totally *** PD controller utilizes states of both the torso(angle and angular velocity)and the stance *** numerical simulations show that,by adopting this controller,a planar biped robot with torso can walk stably on level ground,and that the robot can walk with a wide range of speeds and high energy efciency by changing the control *** period-one gaits,one of which is stable while the other three are unstable,are found by *** to the literature surveyed,we are the first to have a torso only driven biped robot walk stably on level ground.

关键词： biped robot torso control passive dynamic walking PD controller

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Filter design of delayed nonlinear discrete-time Markovian neural networks systems with missing measurements

Filter design of delayed nonlinear discrete-time Markovian n...

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Chinese control Conference (CCC)

作者： Weihua Xu Yang Zhu Qihui Duan Lei Wang State Key Laboratory of Industrial Control Technology Institute of Cyber-Systems and Control Zhejiang University Hangzhou China

ISBN: (纸本)9781479900305

This paper presents a L 2 -L ∞ filtering scheme for nonlinear discrete-time Markovian jumping neural networks with time delay under missing output measurements. By constructing appropriate Lyapunov-Krasovskii functional and utilizing some linear matrix inequality techniques, the mean square stability of the stochastic estimation error systems is guaranteed and a sufficient condition is established to ensure the given L 2 -L ∞ filtering performance. What's more, we provide the design approach of the filter when the delayed states in output measurements are involved or output measurements of the systems can be fully obtained. The gain matrix of the filter can be derived from the solution of a set of linear matrix inequalities. Finally, the simulation proves the availability of the proposed approach.

关键词： Electronic mail Linear matrix inequalities Neural networks Delay effects Time measurement Laboratories industrial control

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Exponential Stabilization of Unstable Diffusion-reaction PDEs with Mixed Boundary Condition

Exponential Stabilization of Unstable Diffusion-reaction PDE...

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第三十二届中国控制会议

作者： Zhigang Ren Chao Xu Jian Chu Department of Control Science and Engineering Zhejiang University The State Key Laboratory of Industrial Control Technology Institute of Cyber-Systems and Control

ISBN: (纸本)9789881563835

In this paper,both the optimal control problems and the stability for the state-feedback of an unstable linear parabolic diffnsion-reaction partical differential equations(PDEs) system with an actuator at the right-hand-side boundary are considered. Using the weak variation approach,we firstly derive the first order necessary conditions for the open loop finite-time horizon ***,we discuss the state-feedback problem of the open loop PDEs system,and get the result of the feedback law through solving a Riccati partial differential ***,with the feedback law,we prove out the exponential stability for the state-feedback problem that is very innovative and instructive by using the lyapunov method.

关键词： Optimal control PDEs Exponential Stability Lagrangian Riccati PDE

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Software Maintenance Optimization Based on Stackelberg Game Methods

Software Maintenance Optimization Based on Stackelberg Game ...

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IEEE International Conference on Software Reliability Engineering Workshops (ISSRE Wksp)

作者： Jing Zhao Yan-Bin Wang Gao-Rong Ning Cheng-Hong Wang Kishor S. Trivedi K-Y Cai Zhen-Yu Zhang Department of Computer Science and Technology Harbin Engineering University China Department of Industrial engineering Harbin Institute of Technology China Department of Automatic Control Beihang University China Department of Electrical and Computer Engineering Duke University USA The State Key Laboratory of Computer Science Chines Academy of Sciences China

Application servers (AS) of virtualized platform may suffer from software aging problem. In this paper, we first formulate the system model including three virtual machines. Two of them act as the main servers, and the third machine acts as the backup node. The motivation of our formulated model is that the relationship between the service provider and the service maintainer is collaborative as well as having different goals between them, the service provider as a leader wants to maximize his system availability, while the service maintainer wants to minimize his maintenance cost. Thus, the problem of maximizing availability and minimizing cost between the service provider and service maintainer is Stackelberg game based. Next, we assume that the AS degradation is caused by resource consumption due to memory leaks for the AS on the active VMs, and we present the system degradation states based on Markov renewal processes. We give the analytical definitions of threshold levels for R alert at each VM, which are used to determine the optimal rejuvenation schedules. In addition, we obtain the steady-state availability expressions for the system and the mean maintenance cost. Finally, we give the Stackelberg strategy with the open-loop information and the solutions for the game theory by a numerical illustration.

关键词： Software Aging Availability Games Maintenance engineering Degradation Computer crashes

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Notes on Sampled-data design for robust control of a single qubit

Notes on Sampled-data design for robust control of a single ...

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3rd IFAC Conference on Intelligent control and Automation Science, ICONS 2013

作者： Wang, Yuanlong Chen, Chunlin Dong, Daoyi State Key Laboratory of Industrial Control Technology Institute of Cyber-Systems and Control Zhejiang University Hangzhou 310027 China Department of Control and System Engineering Nanjing University Nanjing 210093 China School of Engineering and Information Technology University of New South Wales Australian Defence Force Academy Canberra ACT 2600 Australia

ISBN: (纸本)9783902823458

This paper presents several notes on the robust control method proposed in [Dong et al (2013), Sampled-data design for robust control of a single qubit. IEEE Transactions on Automatic control, in press] and improves the original sampling periods so that this control method can be better and more furthermore easily realized. For the case of amplitude damping decoherence, a larger sampling period is presented when the upper bound of the probability of failure is small enough. For the case of phase damping decoherence, a larger sampling period is given when the lower bound of the target coherence is large enough. Furthermore, we provide improved sampling periods for both of the above two cases under the same assumption as that in [Dong et al (2013), Sampled-data design for robust control of a single qubit. IEEE Transactions on Automatic control, in press]. Copyright © 2013 IFAC.

关键词： Robust control

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Unmanned Aerial Vehicles (UAV) Heading Optimal Tracking control Using Online Kernel-based HDP Algorithm

Unmanned Aerial Vehicles (UAV) Heading Optimal Tracking Cont...

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International Joint Conference on Neural Networks

作者： Fuxiao Tan Derong Liu Xinping Guan Bin Luo School of Computer and Information Fuyang Teachers College State Key Laboratory of Management and Control for Complex Systems Institute of Automation Chinese Academy of Sciences School of Electronic Information and Electrical Engineering Shanghai Jiao Tong University School of Computer Science and Technology Anhui University

ISBN: (纸本)9781479914821

UAV can work in places that are dangerous, or not easy to reach for humans. However, due to active control and operating difficulties, it is still a challenge to develop fully autonomous flight in complex environments. This paper applies a novel heuristic dynamic programming for the UAV heading optimal tracking controller design, using kernel-based heuristic dynamic programming (KHDP). Kernel-based HDP is developed by integrating kernel methods and approximately linear dependence (ALD) analysis with the critic learning of HDP algorithm. Compared with conventional HDP where neural networks are widely used and their features were manually designed, the proposed algorithm can obtain better generalization capability and learning efficiency through applying the sparse kernel machine into the critic learning process of HDP algorithm. Simulation and experimental results of UAV heading optimal tracking control problems demonstrate the effectiveness of the proposed kernel-based HDP algorithm.

关键词： Kernel Algorithm design and analysis Heuristic algorithms Equations Vectors Approximation algorithms Mathematical model

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A soft constraint adjustment method based on multi-parametric programming in steady-state target calculation for model predictive control

A soft constraint adjustment method based on multi-parametri...

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Chinese control Conference (CCC)

作者： Xiaozhong Lin Lei Xie Hongye Su Institute of Cyber-Systems & Control State Key Laboratory of Industrial Control Technology Zhejiang University Hangzhou 310027 China

In order to track the result of local economic optimization, steady-state target calculation for Model Predictive control should be implemented. When steady-state target calculation for Model Predictive control becomes infeasible, soft constraints adjustment is involved to make it feasible. The traditional method does not consider that different soft constraints adjustment will affect final economic objective achievement, It is not guaranteed to achieve the optimal economic objective through soft constrains adjustment by choosing different weighted coefficient according to experience, a multi-parametric programming algorithm is proposed to divide the variation range of coefficients of the soft constraint into different regions. It can determine the most suitable soft constraints adjustment to achieve optimal economic objective by comparing economic objective value in the different region. Simulation results of typical control model of a heavy oil fractionator proposed by Royal Dutch/Shell Group showed the effectivity of the proposed algorithm.

关键词： Steady-state Predictive control Economics Optimization Programming Laboratories

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