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Sex Difference in Histopathological and Steroidogenesis Metabolism of Zebrafish After Exposure to Spiromesifen

Environmental Toxicology 2025年第4期40卷 598-607页

作者： Zhang, Jie Yang, Yang Fan, Yongmei Yu, Wang Qian, Le Duan, Manman Zhao, Wentian Chen, Xiangguang Song, Wanhui Li, Xuefeng Wang, Chengju Innovation Center of Pesticide Research Department of Applied Chemistry College of Sciences China Agricultural University Beijing China Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests Ministry of Education School of Tropical Agriculture and Forestry Hainan University Haikou China State Key Laboratory for Biology of Plant Disease and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing China College of Horticulture and Plant Protection Henan University of Science and Technology Henan China Institute of Rural Revitalization Dezhou University Shandong Dezhou China Institute of Eco-Environmental and Soil Sciences Guangdong Academy of Sciences Guangzhou China

Spiromesifen (SPM) is widely used for orchard mites and white fly control. The ecotoxicological data suggested that SPM is highly toxic to fish, but the information about its toxic effect on zebrafish is still obscure. In this study, adult zebrafish were exposed to SPM for 21 days. The plasma sex steroid hormone levels reflected the ratio of 17β-estradiol (E2) to testosterone (T) (E2/T) was significantly increased at 0.50 μg/L of SPM in male fish (2.4-fold, p = 0.049). Following 21 days' post exposure, distinct pathological changes were noted in gonad, males were more sensitive than female, which showed the interstitial connective tissue hyperplasia and widener in testis at 15 μg/L of SPM. In male fish, the relative percentage of spermatozoa was 13% decreased at 30 μg/L of SPM (p = 0.041). Which suggest SPM potential role in disrupting male gonad development. qRT-PCR results suggest that expression of follicle stimulating hormone receptor (fshr) was significantly down regulated in female zebrafish (0.29 fold of control, p = 0.010). Variable importance of projection (VIP) scores indicate the most important features separate in female and male. The different response of steroid level towards SPM between male and female zebrafish may due to the distinct regulation of key genes related in steroidogenesis and metabolism. This study for the first time connects the biochemical and histological to reveal the adverse effects of SPM on adult zebrafish in a sex dependent manner. © 2024 Wiley Periodicals LLC.

关键词： Invertebrates

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On using an aerosol thermodynamic model to calculate aerosol acidity of coarse particles

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Journal of Environmental Sciences 2025年第2期148卷 46-56页

作者： Zhengyang Fang Shuwei Dong Chengpeng Huang Shiguo Jia Fu Wang Haoming Liu He Meng Lan Luo Yizhu Chen Huanhuan Zhang Rui Li Yujiao Zhu Mingjin Tang State Key Laboratory of Organic Geochemistry Guangdong Key Laboratory of Environmental Protection and Resources UtilizationGuangzhou Institute of GeochemistryChinese Academy of SciencesGuangzhou 510640China College of Earth and Planetary Sciences University of Chinese Academy of SciencesBeijing 101408China Longhua Center for Disease Control and Prevention of Shenzhen Shenzhen 518109China School of Atmospheric Sciences and Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai) Sun Yat-sen UniversityZhuhai 519082China Institute for Environmental and Climate Research Jinan UniversityGuangzhou 510632China Qingdao Eco-environment Monitoring Center of Shandong Province Qingdao 266003China Environment Research Institute Shandong UniversityQingdao 266237China

Thermodynamic modeling is still themostwidely usedmethod to characterize aerosol acidity,a critical physicochemical property of atmospheric ***,it remains unclear whether gas-aerosol partitioning should be incorporated when thermodynamicmodels are employed to estimate the acidity of coarse *** this work,field measurements were conducted at a coastal city in northern China across three seasons,and covered wide ranges of temperature,relative humidity and NH_(3) *** examined the performance of different modes of ISORROPIA-II(a widely used aerosol thermodynamic model)in estimating aerosol acidity of coarse and fine *** M0 mode,which incorporates gas-phase data and runs the model in the forward mode,provided reasonable estimation of aerosol acidity for coarse and fine *** to M0,the M1 mode,which runs the model in the forward mode but does not include gas-phase data,may capture the general trend of aerosol acidity but underestimates pH for both coarse and fine particles;M2,which runs the model in the reverse mode,results in large errors in estimated aerosol pH for both coarse and fine particles and should not be used for aerosol acidity ***,M1 significantly underestimates liquid water contents for both fine and coarse particles,while M2 provides reliable estimation of liquid water *** summary,our work highlights the importance of incorporating gas-aerosol partitioning when estimating coarse particle acidity,and thus may help improve our understanding of acidity of coarse particles.

关键词： Aerosol acidity Coarse particles Thermodynamic model Aerosol liquid water ISORROPIA-II Gas-aerosol partitioning

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Discontinuous Modulation and control Strategy for Single-Phase LC Inverter

Discontinuous Modulation and Control Strategy for Single-Pha...

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International Conference of Electrical, Electronic and Networked Energy Systems, EENES 2024

作者： Xu, Wei Song, Shengli Long, Xuehan Miao, Yiru Tang, Mengyang Chongqing Sub-Bureau of Southwest China Regional Air Traffic Management Bureau CAAC Chongqing401120 China Chongqing Airport Group Company Chongqing400044 China State Key Laboratory of Power Transmission Equipment Technology Chongqing University Chongqing400044 China Key Laboratory of Smart Grid and Intelligent Control in Nanchong Southwest Petroleum University Nanchong637001 China

ISBN: (纸本)9789819620791

In order to reduce the switching loss of the single-phase inverter, improve the efficiency and power density, a discontinuous PWM modulation strategy based on the unified modulation method is proposed for the single-phase full bridge LC inverter. The differential mode modulation signal between a-phase and b-phase is constant, the common mode modulation signal is adjusted and injected into the modulation signals of a-phase and b-phase, the switching devices of a-phase and b-phase are in inactive state alternating, so the switching loss can be reduced. Then, the mathematical model of the single-phase full bridge LC inverter is established, the parameter design method of the single loop proportional resonant controller is studied according to the amplitude-frequency characteristic curve, the output voltage can track the reference signal without error under the disturbances of input voltage and load. Finally, the experiment is carried out, which shows that the switching loss can be reduced by the proposed discontinuous PWM modulation, and high quality and precision voltage output can be obtained by the single loop proportional resonant controller. © Beijing Paike Culture Commu. Co., Ltd. 2025.

关键词： Pulse width modulation

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Health of plateau soil environment: Corresponding relationship of heavy metals in different land use/cover types (LULCC)

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Science of the Total Environment 2025年 973卷 179162页

作者： Fu, Zhenghui Liu, Yong Jiang, Xia Guo, Huaicheng Wang, Shuhang Li, Zheng State Key Laboratory of Environmental Criteria and Risk Assessment National Engineering Laboratory for Lake Pollution Control and Ecological Restoration State Environmental Protection Key Laboratory for Lake Pollution Control Chinese Research Academy of Environmental Sciences Beijing100012 China College of Environmental Sciences and Engineering Peking University Beijing100871 China

The study of land use and land cover change (LULCC) in the Qinghai Tibet Plateau is an important part of regional land science and global change science research. Due to their sensitive response to climate change, plateau lakes undergo changes in their surrounding land use types and soil physicochemical properties, which in turn affect soil environmental health. In the context of global climate change, more scholars are focusing on the relationship between climate change and the transformation of land use types, while research on the response relationship between land use types and pollutant accumulation at the end of the entire system is scarce. This study systematically analyzed the bioavailability and migration dynamics of heavy metals in soils of different land use types in the Qinghai Tibet Plateau watershed, providing solid data support for ecological protection and high-quality development of the plateau watershed, and offering new research ideas for water environment security in the Qinghai Tibet Plateau under the background of climate change. © 2025

关键词： Lakes

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Experimental research on enhanced the microfine oil droplets separation using hydrocyclone coupled with fiber coalescence

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Chinese Journal of Chemical Engineering 2025年 82卷 15-24页

作者： Zhang, Lian Lu, Zhaojin Ma, Likun Bai, Zhishan State Environmental Protection Key Laboratory of Environmental Risk Assessment and Control on Chemical Process School of Mechanical and Power Engineering East China University of Science and Technology Shanghai200237 China

The limitations of swirl separation in removing microfine oil droplets in water have driven the development of hydrocyclone technology coupled with multiphase or multifield techniques. To enhance microfine oil droplets separation, a novel hydrocyclone separation coupled with fiber coalescence (HCCFC) was designed. The interaction between fiber balls and oil droplets inside the hydrocyclone, including droplet coalescence and breakage, was investigated. The influence of different operating parameters on separation efficiency was discussed. The results showed that fiber balls promoted oil droplet coalescence when the inlet droplet size (D43) was below 22.37 μm but caused droplet breakage above this threshold. The coalescence performance of HCCFC improved with increasing inlet oil content but declined beyond 450 mg·L−1. Separation experiments confirmed that HCCFC outperformed conventional hydrocyclone, with separation efficiency increasing by 2.9% to 20.0%. As the fiber ball content and inlet flow rate increased, the separation efficiency showed a trend of first increasing and then decreasing. Additionally, HCCFC's separation efficiency varied with inlet oil droplet size distribution, showing the most significant enhancement when D43 was 22.37 μm, where separation efficiency increased by 14.4%. These findings offer insights into the development and application of multiphase coupled with hydrocyclone technology. © 2025 Chemical Industry and Engineering Society of China, and Chemical Industry Press Co., Ltd.

关键词： Chemical industry

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Hydrochemistry characteristics and genesis of shallow groundwater in diverse industrial agglomeration areas in typical alluvial plain of the Yellow River

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Science of the Total Environment 2025年 958卷 177764页

作者： Zhang, Fan Yuan, Ying Xi, Beidou Lu, Haojie Jiang, Yu Hui, Kunlong Tan, Wenbing Wang, Hui Meng, Fanhua State Key Laboratory of Environmental Criteria and Risk Assessment Chinese Research Academy of Environmental Sciences Beijing100012 China State Environmental Protection Key Laboratory of Simulation and Control of Groundwater Pollution Chinese Research Academy of Environmental Sciences Beijing100012 China

The groundwater environment in the middle and upper reaches of the Yellow River in China has attracted extensive attention. But, the hydrochemistry characteristics and genesis of shallow groundwater in diverse industrial agglomeration areas in typical alluvial plain of the Yellow River are still unknown. In this study, geochemical analysis methods, the positive matrix factorisation model, and the geodetector model were used to multidimensional analyze. The results showed that 78 % of the groundwater samples had high-alkalinity-risk and high-salinity-risk, and the water quality was poor and unsuitable for irrigation. "Very poor" and "undrinkable" samples accounted for 26.31 %. The weathering of calcium magnesium minerals and the dissolution of soluble sulfate were important sources of the main chemical components of the groundwater. However, evaporation and concentration gradually dominated from the inclined plain of the piedmont to the alluvial plain of the Yellow River. Domestic and agricultural activities were important sources of nitrogen species (NO3−, NO2−, and NH4+). The presence of Al, Mn, Zn, and Pb was caused by the production activities of various enterprises. The pollution contributors of natural sources, domestic and agricultural activities, industrial production and other sources were 29.49 %, 17.05 %, 31.41 %, and 22.05 %, respectively. Groundwater velocity and enterprise type were the main factors driving groundwater pollution by these four metal substances. The interaction between enterprise type and groundwater velocity had the greatest influence on the concentrations of Al, Mn, and Zn. Moreover, the interaction between enterprise type and the distance from enterprise had the greatest influence on Pb levels. Overall, groundwater pollution in the study area was the result of both natural and human factors. The findings of this study can offer valuable insights and theoretical support for preventing groundwater pollution in other industrial agglomerat

关键词： Groundwater geochemistry

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Surface water and groundwater interactions contributed the seasonal NO3− and NH4+ sources and transformations in a hilly region, China

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Journal of Environmental Management 2025年 387卷 125750页

作者： Wang, Jun Niu, Yuan Li, Chang Liu, Zian Jiang, Xia Wu, Yali State Key Laboratory of Environmental Criteria and Risk Assessment National Engineering Laboratory for Lake Pollution Control and Ecological Restoration Beijing100012 China State Environmental Protection Key Laboratory for Lake Pollution Control Chinese Research Academy of Environmental Sciences Beijing100012 China College of Resources and Environmental Henan Agricultural University Zhengzhou450046 China

Comprehensive understanding of nitrogen (N) sources and transformations under the interactions of variable water cycle processes were of great significance for formulating nitrogen mitigation strategies in aquatic systems. Multiple stable isotopes (δ15N-NO3-, δ18O-NO3-, δ15N-NH4+, δ2H-H2O, and δ18O-H2O) and hydrochemical characteristics in surface water (SW) and groundwater (GW) were to reveal seasonal NO3− and NH4+ sources and transformations in a hilly region with frequent vertical movements in this study. The δ2H-H2O, δ18O-H2O, and hydrochemical analysis highlighted surface water and groundwater interactions. The correlation between δ15N-NO3- and d-excess demonstrated soil leaching. Nitrification was the predominant nitrogen transformation process in the watershed during both seasons although denitrification occurred in certain points. Primary source of NO3− in SW was soil nitrogen (SN;34.5 ± 14.9 % in the dry season and 76.4 ± 8.1 % in the wet season). The contributions of various sources to NH4+ in SW were relatively equal during the dry season, while GW (30.5 ± 19.3 %) and manure and sewage (M&S) (29.0 ± 15.2 %) were the primary sources during the wet season. This study proved that groundwater was a significant source for NO3− and NH4+ input when utilizing source-track models, especially in hilly regions. These findings improve input sources for mixture models and identify the sources and transformations of water cycling and N biogeochemical processes. © 2025

关键词： Water analysis

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Uncoupling of nutrient metabolism and cellular redox by cytosolic routing of the mitochondrial G-3-P dehydrogenase Gpd2 causes loss of conidiation and pathogenicity in Pyricularia oryzae

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Journal of Integrative Agriculture 2025年第2期24卷 638-654页

作者： Wenqin Fang Yonghe Hong Tengsheng Zhou Yangdou Wei Lili Lin Zonghua Wang Xiaohan Zhu State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops College of Plant ProtectionFujian Agriculture and Forestry UniversityFuzhou 350002China Department of Biology College of Arts and ScienceUniversity of SaskatchewanSaskatoonSK S7N5E2Canada Fuzhou Institute of Oceanography Minjiang UniversityFuzhou 350108China Department of Plant Science University of ManitobaWinnipegMB R3T2N2Canada

Oxidation of self-stored carbohydrates and lipids provides the energy for the rapid morphogenetic transformation during asexual and infection-related development in Pyricularia oryzae,which results in intracellular accumulation of reducing equivalents NADH and FADH_(2),requiring a cytosolic shuttling machinery towards *** previous studies identified the mitochondrial D-lactate dehydrogenase MoDld1 as a regulator to channel the metabolite flow in conjunction with redox ***,the regulator(s)facilitating the cytosolic redox balance and the importance in propelling nutrient metabolite flow remain *** G-3-P shuttle is a conserved machinery transporting the cytosolic reducing power to *** ***,the mitochondrial G-3-P dehydrogenase Gpd2 was required for cellular NAD^(+)/NADH balance and fungal *** this study,we relocate the mitochondrial G-3-P dehydrogenase Gpd2 to the cytosol for disturbing cytosolic redox *** results showed overexpression of cytosolic gpd2^(Δmts)without the mitochondrial targeted signal(MTS)driven by Ribosomal protein 27 promoter(PR27)exerted conflicting regulation of cellular oxidoreductase activities compared to theΔModld1 deletion mutant by RNA-seq and prevented the conidiation and pathogenicity of ***,overexpression of gpd2^(Δmts)caused defects in glycogen and lipid mobilization underlying asexual and infectious structural development associated with decreased cellular NADH production and weakened anti-oxidation ***-seq and non-targeted metabolic profiling revealed down-regulated transcriptional activities of carbohydrate metabolism and lower abundance of fatty acids and secondary metabolites in RP27:gpd2^(Δmts).Thus,our studies indicate the essential role of cytosolic redox control in nutrient metabolism fueling the asexual and infection-related development in ***.

关键词： Pyricularia oryzae mitochondrial G-3-P dehydrogenase NAD+/NADH balance fungal virulence mitochondrial targeted signal

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Ecological Mechanisms of Carbon Sequestration in Vegetated Coastal Wetland Ecosystem: Exploring the Roles of Biodiversity and Environmental Changes

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Journal of Marine Environmental Engineering 2025年第1期12卷 35-47页

作者： Liu, Lele Du, Ning Eller, Franziska Ye, Siyuan Li, Xiaowen Wei, Jifang Guo, Yaolin Brix, Hans Guo, Weihua Key Laboratory of Ecological Prewarning Protection and Restoration of Bohai Sea Ministry of Natural Resources School of Life Sciences Shandong University 72 Binhai Road Qingdao266237 China Department of Biology Aarhus University Ole Worms Alle 1 Aarhus8000 Denmark Key Laboratory of Coastal Wetland Biogeosciences Qingdao Institute of Marine Geology China Geological Survey Qingdao266000 China School of Environment State Key Joint Laboratory of Environment Simulation and Pollution Control Beijing Normal University Beijing100875 China North China Sea Ecological Center of the Ministry of Natural Resources Qingdao266000 China Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering National Observations and Research Station for Wetland Ecosystems of the Yangtze Estuary Institute of Biodiversity Science and Institute of EcoChongming School of Life Sciences Fudan University Shanghai200433 China

Vegetated coastal wetlands, also called blue carbon ecosystems, are significant carbon sinks that play a vital role in global climate change mitigation. Understanding the ecological mechanisms of carbon sequestration in these ecosystems is essential to effectively manage and conserve them. This review focuses on macroecological mechanisms of carbon transfer and microecological mechanisms of carbon stabilization in vegetated coastal wetland ecosystems. Macroecological mechanisms include the horizontal and vertical carbon transport coupled to plant ecophysiology and community diversity, while microecological mechanisms include processes related to soil organic matter dynamics, such as decomposition, stabilization, and mineralization. key scientific issues surrounding these mechanisms include transformation between carbon sink and source, stabilization mechanisms of organic carbon, and environmental effects at multiple time scales. A comprehensive research framework is also presented, which integrates spatial-temporal pattern, macroecological mechanism, microecological mechanism, and ecological engineering. Addressing these key scientific questions within the proposed framework can provide valuable insights into the significant role of biodiversity in blue carbon ecosystems, as well as significant implications for management and conservation of vegetated coastal wetlands under global climate changes. © 2025 Old City Publishing, Inc.

关键词： Ecosystems

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The phytoplasma(Candidatus Phytoplasma arecae)is the crucial pathogen to cause areca palm yellow leaf disease

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Science Bulletin 2025年第6期70卷 847-851页

作者： Shuangwei Song Qian Wang Liangxiao Huo Liqiang Xie Jiaqi Chen Hongguang Cui Zhaoji Dai Jinrui Kang Yi Li Wei Guo Jinfeng Chen Le Kang Xiaoming Zhang State Key Laboratory of Integrated Management of Pest Insects and Rodents Institute of ZoologyChinese Academy of SciencesBeijing 100101China College of Life Science University of Chinese Academy of SciencesBeijing 100049China College of Life Sciences Hebei UniversityBaoding 071002China Hainan Seed Industry Laboratory Sanya 572025China Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests(Ministry of Education) School of Tropical Agriculture and ForestryHainan UniversityHaikou 570228China The State Key Laboratory of Protein and Plant Gene Research School of Life SciencesPeking UniversityBeijing 100871China State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops Vector-borne Virus Research CenterCollege of Plant ProtectionFujian Agriculture and Forestry UniversityFuzhou 350002China

The areca palm(Areca catechu L.)is a vital tropical forest species widely utilized for vegetation restoration,landscaping,and greening *** nuts hold significant importance in traditional medicine,serving as remedies for various ailments such as parasitic infections,digestive disorders,and depression[1–3].Areca palm yellow leaf disease(AYLD)poses a serious threat to areca palm growth.

关键词： Areca palm yellow leaf disease Candidatus phytoplasma arecae traditional medicineserving phytoplasma areca palm areca palm areca parasitic infectionsdigestive disordersand Areca catechu

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