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A deep learning-based image analysis for assessing the extent of abduction in abducens nerve palsy patients before and after strabismus surgery

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Advances in Ophthalmology Practice and Research 2024年第4期4卷 202-208页

作者： Ziying Zhou Shengqiang Shi Xiajing Tang Zhaoyang Xu Juan Ye Xingru Huang Lixia Lou Eye Center The Second Affiliated Hospital of Zhejiang University School of MedicineZhejiang Provincial Key Laboratory of OphthalmologyZhejiang Provincial Clinical Research Center for Eye DiseasesZhejiang Provincial Engineering Institute on Eye DiseasesHangzhouChina Hangzhou Dianzi University HangzhouChina Wellcome-MRC Cambridge Stem Cell Institute University of CambridgeCambridgeUnited Kingdom School of Electronic Engineering and Computer Science Queen Mary University of LondonLondonUnited Kingdom

Purpose:This study aimed to propose a novel deep learning-based approach to assess the extent of abduction in patients with abducens nerve palsy before and after strabismus ***:This study included 13 patients who were diagnosed with abducens nerve palsy and underwent strabismus surgery in a tertiary *** of primary,dextroversion and levoversion position were collected before and after strabismus *** eye location and eye segmentation network were trained via recurrent residual convolutional neural networks with attention gate connection based on U-Net(R2AU-Net).Facial images of abducens nerve palsy patients were used as the test set and parameters were measured automatically based on the masked *** abduction also was measured manually,and relative abduction was *** between manual and automatic measurements,as well as repeated automatic measurements were *** and postoperative results were ***:The intraclass correlation coefficients(ICCs)between manual and automatic measurements of absolute abduction ranged from 0.985 to 0.992(P<0.001),and the bias ranged from-0.25 mm to-0.05 *** ICCs between two repeated automatic measurements ranged from 0.994 to 0.997(P<0.001),and the bias ranged from-0.11 mm to 0.05 *** strabismus surgery,absolute abduction of affected eye increased from 2.18±1.40 mm to 3.36±1.93 mm(P<0.05).The relative abduction was improved in 76.9%patients(10/13)after surgery(P<0.01).Conclusions:This image analysis technique demonstrated excellent accuracy and repeatability for automatic measurements of ocular abduction,which has promising application prospects in objectively assessing surgical outcomes in patients with abducens nerve palsy.

关键词： Abducens nerve palsy Deep learning Automatic image analysis Surgery

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A single-nucleus transcriptomic atlas of primate testicular aging reveals exhaustion of the spermatogonial stem cell reservoir and loss of Sertoli cell homeostasis

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Protein & cell 2023年第12期14卷 888-907页

作者： Daoyuan Huang Yuesheng Zuo Chen Zhang Guoqiang Sun Ying Jing Jinghui Lei Shuai Ma Shuhui Sun Huifen Lu Yusheng Cai Weiqi Zhang Fei Gao Andy Peng Xiang Juan Carlos Izpisua Belmonte Guang-Hui Liu Jing Qu Si Wang Advanced Innovation Center for Human Brain Protection National Clinical Research Center for Geriatric DisordersXuanwu Hospital Capital Medical UniversityBeijing100053China State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Aging Translational Medicine Center International Center for Aging and CancerBeijing Municipal Geriatric Medical Research CenterXuanwu HospitalCapital Medical UniversityBeijing 100053China State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Beijing Institute for Stem Cell and Regenerative Medicine Beijing 100101China CAS Key Laboratory of Genomic and Precision Medicine Beijing Institute of GenomicsChinese Academy of SciencesBeijing 100101China Institute for Stemcell and Regeneration CAsBeijing100101China China National Center for Bioinformation Beijing 100101China The Fifth People's Hospital of Chongqing Chongqing 400062China 11Sino-Danish College University of Chinese Academy of SciencesBeijing 101408China Sino-Danish Center for Education and Research Beijing 101408China Center for Stem Cell Biology and Tissue Engineering Key Laboratory for Stem Cells and Tissue EngineeringMinistry of EducationSun Yat-sen UniversityGuangzhou510000China Department of Biochemistry Zhongshan School of MedicineSun Yat-sen UniversityGuangzhou 510000China Altos Labs Inc.San DiegoCA 92121USA and Aging Biomarker Consortium China

The testis is pivotal for male reproduction,and its progressive functional decline in aging is associated with ***,the regulatory mechanism underlying primate testicular aging remains largely ***,we resolve the aging-related cellular and molecular alterations of primate testicular aging by establishing a single-nucleus transcriptomic ***-expression patterns along the spermatogenesis trajectory revealed molecular programs associated with attrition of spermatogonial stem cell reservoir,disturbed meiosis and impaired spermiogenesis along the sequential ***,Sertoli cell was identified as the cell type most susceptible to aging,given its deeply perturbed age-associated transcriptional ***,downregulation of the transcription factor Wilms'Tumor 1(WTi),essential for Sertoli cell homeostasis,was associated with accelerated cellular senescence,disrupted tight junctions,and a compromised cell identity signature,which altogether may help create a hostile microenvironment for ***,our study depicts in-depth transcriptomic traits of non-human primate(NHP)testicular aging at single-cell resolution,providing potential diagnostic biomarkers and targets for therapeutic interventions against testicular aging and age-related male reproductive diseases.

关键词： single-nucleus RNA sequencing primate testis aging WT1

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Enhancement of SARS-CoV-2 vaccine-induced immunity by a Toll-like receptor 7 agonist adjuvant

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Signal Transduction and Targeted Therapy 2023年第6期8卷 2481-2484页

作者： Gen Li Meixing Yu Qiong Ke Jing Sun Yanwen Peng Chuanfeng Xiong Olivia Monteiro COVID-Infection and Immunity Investigation Group Jincun Zhao Andy P.Xiang Guangzhou Women and Children’s Medical Center Guangzhou Medical UniversityGuangzhouChina Center for Stem Cell Biology and Tissue Engineering Key Laboratory for Stem Cells and Tissue EngineeringMinistry of EducationSun Yat-sen UniversityGuangzhouChina State Key Laboratory of Respiratory Disease National Clinical Research Center for Respiratory DiseaseGuangzhou Institute of Respiratory HealthFirst Affiliated HospitalGuangzhou Medical UniversityGuangzhouChina Center for Biomedicine and Innovations Faculty of MedicineMacao University of Science and TechnologyMacaoChina 不详

Dear Editor,Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)is an enveloped positive-sense single-stranded RNA(ssRNA)virus responsible for the global COVID-19 *** vaccines for SARS-CoV-2 have been developed and applied worldwide,research on vaccine adjuvant is *** infections normally elicit a range of innate immune responses through the activation of pattern recognition receptors(PRRs).

关键词： immunity vaccine pattern

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FOXP4 promotes proliferation of human spermatogonial stem cells

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Asian Journal of Andrology 2023年第3期25卷 322-330页

作者： Shu-Wei Luo Le Tang Dai Zhou Hao Bo Li-Qing Fan Reproductive Medicine Center Hunan Provincial Maternal and Child Health Care HospitalChangsha 410000China NHC Key Laboratory of Birth Defect for Research and Prevention Hunan Provincial Maternal and Child Health Care HospitalChangsha 410000China Institute of Reproduction and Stem Cell Engineering School of Basic Medicine ScienceCentral South UniversityChangsha 410000China Reproductive and Genetic Hospital of CITIC-Xiangya Changsha 410000China

Continuous self-renewal and differentiation of spermatogonial stem cells(SSCs)is vital for maintenance of adult *** several spermatogonial stem cell regulators have been extensively investigated in rodents,regulatory mechanisms of human SSC self-renewal and differentiation have not been fully *** analyzed single-cell sequencing data from the human testis and found that forkhead box P4(FOXP4)expression gradually increased with development of *** analysis of its expression patterns in human testicular tissues revealed that FOXP4 specifically marks a subset of spermatogonia with stem cell *** inactivation of FOXP4 in human SSC lines suppressed SSC proliferation and significantly activated ***4 expressions were markedly suppressed in tissues with dysregulated *** findings imply that FOXP4 is involved in human SSC proliferation,which will help elucidate on the mechanisms controlling the fate decisions in human SSCs.

关键词： forkhead box P4 human proliferation spermatogonial stem cells testis

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Human CD56+CD39+ dNK cells support fetal survival through controlling trophoblastic cell fate:immune mechanisms of recurrent early pregnancy loss

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National Science Review 2024年第6期11卷 129-145页

作者： Wentong Jia Liyang Ma Xin Yu Feiyang Wang Qian Yang Xiaoye Wang Mengjie Fan Yan Gu Ran Meng Jian Wang Yuxia Li Rong Li Xuan Shao Yan-Ling Wang State Key Laboratory of Stem cell and Reproductive Biology Key Laboratory of Organ Regeneration and ReconstructionInstitute of ZoologyChinese Academy of Sciences Beijing Institute for Stem Cell and Regenerative Medicine University of the Chinese Academy of Sciences NHC Key Lab of Reproduction Regulation Shanghai Engineering Research Center of Reproductive Health Drug and Devices Shanghai Institute for Biomedical and Pharmaceutical Technologies National Clinical Center for Obstetrics and Gynecology Peking University Third Hospital Department of Family Planning The Second Hospital of Tianjin Medical University Department of Prenatal Screening Haidian Maternal and Child Health Hospital

Decidual natural killer（dNK） cells are the most abundant immune cells at the maternal-fetal interface during early pregnancy in both mice and humans, and emerging single-cell transcriptomic studies have uncovered various human dNK subsets that are disrupted in patients experiencing recurrent early pregnancy loss（RPL） at early gestational stage, suggesting a connection between abnormal proportions or characteristics of dNK subsets and RPL pathogenesis. However, the functional mechanisms underlying this association remain unclear. Here, we established a mouse model by adoptively transferring human dNK cells into pregnant NOG（NOD/Shi-scid/IL-2Rγnull） mice, where human dNK cells predominantly homed into the uteri of recipients. Using this model, we observed a strong correlation between the properties of human dNK cells and pregnancy outcome. The transfer of dNK cells from RPL patients（dNK-RPL）remarkably worsened early pregnancy loss and impaired placental trophoblast cell differentiation in the recipients. These adverse effects were effectively reversed by transferring CD56+CD39+dNK *** studies revealed that CD56+CD39+dNK subset facilitates early differentiation of mouse trophoblast stem cells（m TSCs） towards both invasive and syncytial pathways through secreting macrophage colony-stimulating factor（M-CSF）. Administration of recombinant M-CSF to NOG mice transferred with dNK-RPL efficiently rescued the exacerbated pregnancy outcomes and fetal/placental development. Collectively, this study established a novel humanized mouse model featuring functional human dNK cells homing into the uteri of recipients and uncovered the pivotal role of M-CSF in fetal-supporting function of CD56+CD39+dNK cells during early pregnancy, highlighting that M-CSF may be a previously unappreciated therapeutic target for intervening RPL.

关键词： recurrent pregnancy loss CD56+CD39+ dNK subset NOG mice adoptive transfer placental trophoblast cell fate M-CSF

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Ganoboninketal C from Ganoderma boninense improves the efficacy of CDDP-based chemotherapy through inhibiting translesion DNA synthesis

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Food Science and Human Wellness 2024年第5期13卷 2982-2992页

作者： Xiaolu Ma Fei Yang Ke Ma Hongyan Shen Junjie Han Kai Wang Yeran Yang Jiawei Zhu Ruiyuan An Qilin Wang Tie-Shan Tang Bo Zhou Hongwei Liu Caixia Guo College of Biomedical Engineering Taiyuan University of TechnologyTaiyuan 030024China CAS Key Laboratory of Genomics and Precision Medicine Beijing Institute of Genomics/China National Center for BioinformationUniversity of Chinese Academy of SciencesChinese Academy of SciencesBeijing 100101China State Key Laboratory of Membrane Biology Institute of ZoologyUniversity of Chinese Academy of SciencesChinese Academy of SciencesBeijing 100101China Institute of Microbiology University of Chinese Academy of SciencesChinese Academy of SciencesBeijing 100101China Beijing Institute for Stem Cell and Regenerative Medicine Beijing 100101China University of Chinese Academy of Sciences Chinese Academy of SciencesBeijing 100101China

Translesion DNA synthesis(TLS)can bypass DNA lesions caused by chemotherapeutic drugs,which usually result in drug *** its key role in mutagenesis and cell survival after DNA damage,inhibition of the TLS pathway has emerged as a potential target for improving the efficacy of DNA-damaging agents such as cisplatin(CDDP),a widely used anticancer ***,few suitable natural TLS inhibitors have been ***,we found that a triterpenoid compound Ganoboninketal C(26-3)from Ganoderma boninense,a traditional Chinese medicine,can impair CDDP-induced TLS polymerase eta(Polη)focus formation,PCNA monoubiquitination as well as ***,26-3 can significantly sensitize tumor cells to CDDP killing and reduce the proportion of cancer stem cells in AGS and promote apoptosis after CDDP ***,26-3 can also sensitize tumor cells to Gefitinib ***,through RNA-seq analysis,we found that 26-3 could abrogate the CDDP-induced upregulation of Polηand PIDD(p53-induced protein with a death domain),2 known factors promoting TLS ***,we found that activating transcription factor 3 is a potential novel TLS *** together,we have identified a natural TLS inhibitor 26-3,which can be potentially used as an adjuvant to improve clinical efficacy.

关键词： Ganoderma boninense Ganoboninketal C Cisplatin chemotherapy Translesion DNA synthesis

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A long-ignored skeletal tissue filled with oil

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Science (New York, N.Y.) 2025年第6730期387卷 136-137页

作者： Viviana Hermosilla Aguayo Licia Selleri Program in Craniofacial Biology Department of Orofacial Sciences Department of Anatomy Institute for Human Genetics Eli and Edythe Broad Center of Regeneration Medicine and Stem Cell Research University of California San Francisco San Francisco CA USA.

Lipid-rich cartilage points to nonmetabolic functions of lipid vacuoles in mammals.

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Gut microbiota plays pivotal roles in benign and malignant hematopoiesis

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Blood Science 2024年第4期6卷 13-21页

作者： Yuxuan Li Biao Zhang Lingli Jiang Tao Cheng Hui Cheng Pengxu Qian State Key Laboratory of Experimental Hematology National Clinical Research Center for Blood DiseasesHaihe Laboratory of Cell EcosystemInstitute of Hematology&Blood Diseases HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeTianjin 300020China Tianjin Institutes of Health Science Center for Stem Cell MedicineChinese Academy of Medical SciencesDepartment of Stem Cell&Regenerative MedicinePeking Union Medical CollegeTianjin 300020China Center for Stem Cell and Regenerative Medicine and Bone Marrow Transplantation Center of the First Affiliated Hospital Zhejiang University School of MedicineHangzhou 310058China Liangzhu Laboratory Zhejiang University1369 West Wenyi RoadHangzhou 311121China Institute of Hematology Zhejiang University&Zhejiang Engineering Laboratory for Stem Cell and ImmunotherapyHangzhou 310058China

Accumulated evidence emerges that dynamic changes in human gut microbiota and microbial metabolites can alter the ecological balance of symbiotic *** gut microbiota plays a role in various diseases through different *** and more attention has been paid to the effects that human microbiota extends beyond the *** review summarized the current understanding of the roles that gut microbiota plays in hematopoietic regulation and the occurrence and development of benign and malignant hematologic *** progress of the application of microbiota in treatment was discussed in order to provide new insights into clinical diagnosis and treatment in the future.

关键词： Fecal microbiota transplantation Graft-versus-host disease Gut microbiota Hematologic diseases Hematopoietic stem cells

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engineering of Small Drug Molecule/Anionic Polysaccharide Interactions for Development of Physical Drug-Delivery Systems with High Loading Capacity and Tunable Release Profile

SSRN

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SSRN 2023年

作者： Daemi, Hamed Mohammadian-Sabet, Fariba Sobhkhiz, Zahra Nazemi, Zahra Shayesteh, Alireza Angji, Mahmoud Torabi Baharvand, Hossein Department of Cell Engineering Cell Science Research Center Royan Institute for Stem Cell Biology and Technology ACECR Tehran Iran Chemical Company Tehran Iran School of Chemistry College of Science University of Tehran Tehran Iran Department of Chemistry State University of New York at Oswego OswegoNY13126 United States School of Chemical Engineering College of Engineering University of Tehran Tehran Iran Royan Institute for Stem Cell Biology and Technology Iran

Research on stimuli-responsive drug delivery systems (SDDS) is increasingly focusing on more efficient loading and controlled release of pharmaceuticals by modifying physical interactions. Here, we used the "hard and soft acids and bases" (HSAB) theory to develop a new approach that can be employed to design SDDS with high drug loading capacity and highly tunable drug release profile. We will show that sulfated alginate as an anionic soft binder have higher affinity toward cationic soft drugs compared to the hard alginate binder. Surprisingly, the maximum adsorption capacity of the sulfated anionic binder toward cationic malachite green drug (based on Langmuir model) reached up to 1248.13 mg.g-1 in a short time (30 min), which was significantly higher than previously reported polysaccharide binders. Density functional theory calculations revealed that the sulfate anion with a hardness of 3.38 eV is softer than the carboxylate anion, and binds more strongly to the soft malachite green drug. Furthermore, the encapsulation efficiency of tetracycline hydrochloride entrapped by the soft sulfated alginate was 36%, while approximately no complex was formed when alginate was used as the hard binder. © 2023, The Authors. All rights reserved.

关键词： Density functional theory

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Jellyfish Tentacle‑Inspired Hydrogel Microfibers Implanted with Discrete Structural Color Microsphere Tactile Sensing Units

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Advanced Fiber Materials 2022年第5期4卷 1209-1218页

作者： Jiahui Guo Han Zhang Hui Zhang Hanxu Chen Zhuxiao Gu Dagan Zhang Yuanjin Zhao Department of Rheumatology and Immunology Nanjing Drum Tower HospitalSchool of Biological Science and Medical EngineeringSoutheast UniversityNanjing 210096China Oujiang Laboratory(Zhejiang Lab for Regenerative Medicine Vision and Brain Health)Wenzhou InstituteUniversity of Chinese Academy of SciencesWenzhou 325001ZhejiangChina Institute for Stem Cell and Regeneration Chinese Academy of ScienceBeijing 100101China

Tactile sensors with distinctive ability to imitate skins have attracted considerable attention from researches for applications in a variety of sensing ***,inspired by the tentacles of jellyfish,biomimetic hydrogel microfibers were fabricated to be implanted with discrete structural color microsphere units for spatial tactile *** employing a microfluidic spinning technology,the generated microfibers were with high microsphere encapsulation features and controllable morphologies because of the density match of microspheres and the pre-hydrogel *** addition,benefitting from the easy manipulation of the microfluidics,microfibers implanted with different structural color microspheres could also be *** was demonstrated that the resultant microfibers would show synchronous shifts of photonic bandgaps as well as structural color when a local force like pressure or tension was applied to the microsphere *** on the localization of finger bending experiments,the practical values of the bioinspired microfibers have also been proved as spatial tactile ***,it is believed that the proposed bioinspired hydrogel microfibers are greatly significant in diverse sensing application fields.

关键词： Bioinspired Microfluidics Microfiber Structural color Tactile sensor

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