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Meta-Analysis of the Expansion in the Field of Structural Biology of ABC Transporters

BioDesign Research 2022年第1期2022卷 124-135页

作者： Soomi Kim Teena Bajaj Cole Chabon Eric Tablante Tatyana Kulchinskaya Tae Seok Moon Ruchika Bajaj Stem Cell Technology Certificate Program City College of San FranciscoUSA Biotechnology Certificate Program City College of San FranciscoUSA Comparative Biochemistry Program University of California BerkeleyUSA Department of Energy Environmental and Chemical EngineeringWashington University in St.LouisUSA Department of Bioengineering and Therapeutics Sciences University of California San FranciscoUSA

ABC transporters are molecular machines which power the solute transport using ATP *** structural biology of ABC transporters has been exploding for the last few years,and this study explores timelines and trends for various attributes such as structural tools,resolution,fold,sources,and group *** study also evidences the significance of mammalian expression systems,advancements in structural biology tools,and the developing interest of group leaders across the world in the remarkably progressing *** field started in 2002 and bloomed in 2016,and COVID years were really productive to the ***,the study explores 337 structures of 58 unique ABC transporters deposited in the PDB database from which P-glycoprotein has the largest number of ***,62%of total structures are determined at the resolution of 3-4Åand 53%of structures belong to fold IV *** progressive advancements in the field,the field is shifting from prokaryotic to eukaryotic sources and X-ray crystallography to cryoelectron *** the nutshell,this study uniquely provides the detailed snapshot of the field of structural biology of ABC transporters with real-time data.

关键词： uniquely really shifting

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engineering biomimetic intestinal topological features in 3D tissue models: retrospects and prospects

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Bio-Design and Manufacturing 2021年第3期4卷 568-595页

作者： Tarun Agarwal Valentina Onesto Lallepak Lamboni Aafreen Ansari Tapas K.Maiti Pooyan Makvandi Massoud Vosough Guang Yang Department of Biotechnology Indian Institute of Technology KharagpurKharagpurWest Bengal 721302India Center for Advanced Biomaterials for Health Care Istituto Italiano di Tecnologia(IIT@CRIB)80125 NaplesItaly National Engineering Research Center for Nano-Medicine Department of Biomedical EngineeringCollege of Life Science and TechnologyHuazhong University of Science and Technology1037 Luoyu RoadWuhan 430074People’s Republic of China Laboratoire de Biologie Moléculaire Institut National d’Hygiène-Togo26 Rue NangbétoQuartier Administratif-PO.Box 1396LoméTogo Department of Biotechnology and Medical Engineering National Institute of Technology RourkelaRourkelaOrissa 769008India Institute for Polymers Composites and Biomaterials(IPCB)National Research Council(CNR)NaplesItaly Department of Stem Cells and Developmental Biology Cell Science Research CenterRoyan Institute for Stem Cell Biology and TechnologyACECRTehranIran Department of Regenerative Medicine Cell Science Research CentreRoyan Institute for Stem Cell Biology and TechnologyACECRTehranIran

Conventional 2D intestinal models cannot precisely recapitulate biomimetic features in vitro and thus are unsuitable for various pharmacokinetic applications,development of disease models,and understanding the host-microbiome ***,recently,efforts have been directed toward recreating in vitro models with intestine-associated unique 3D crypt-villus(for small intestine)or crypt-lumen(for large intestine)*** review comprehensively delineates the current advancements in this research area in terms of the different microfabrication technologies(photolithography,laser ablation,and 3D bioprinting)employed and the physiological relevance of the obtained models in mimicking the features of native intestinal tissue.A major thrust of the manuscript is also on highlighting the dynamic interplay between intestinal cells(both the stem cells and differentiated ones)and different biophysical,biochemical,and mechanobiological cues along with interaction with other cell types and intestinal microbiome,providing goals for the future developments in this *** article also manifests an outlook toward the application of induced pluripotent stem cells in the context of intestinal tissue *** a concluding note,challenges and prospects for clinical translation of 3D patterned intestinal tissue models have been discussed.

关键词： Intestine tissue models Microfabrication Biophysicochemical and biomechanical cues Coculture Induced pluripotent stem cells

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Human umbilical cord-derived mesenchymal stem cells promote repair of neonatal brain injury caused by hypoxia/ischemia in rats

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Neural Regeneration Research 2022年第11期17卷 2518-2525页

作者： Yang Jiao Yue-Tong Sun Nai-Fei Chen Li-Na Zhou Xin Guan Jia-Yi Wang Wen-Juan Wei Chao Han Xiao-Lei Jiang Ya-Chen Wang Wei Zou Jing Liu Stem Cell Clinical Research Center National Joint Engineering LaboratoryRegenerative Medicine CenterThe First Affiliated Hospital of Dalian Medical UniversityDalianLiaoning ProvinceChina Dalian Innovation Institute of Stem Cells and Precision Medicine DalianLiaoning ProvinceChina Department of Neurology The First Affiliated Hospital of Dalian Medical UniversityDalianLiaoning ProvinceChina College of Life Science Liaoning Normal UniversityDalianLiaoning ProvinceChina

Administration of human umbilical cord-derived mesenchymal stem cells(hUC-MSCs)is believed to be an effective method for treating neurodevelopmental disorde *** this study,we investigated the possibility of hUC-MSCs treatment of neonatal hypoxic/ischemic brain injury associated with maternal immune activation and the underlying *** established neonatal rat models of hypoxic/ischemic brain injury by exposing pregnant rats to lipopolysaccharide on day 16 or 17 of *** offspring were intranasally administe red hUC-MSCs on postnatal day *** found that polypyrimidine tract-binding protein-1(PTBP-1)participated in the regulation of lipopolysaccharide-induced maternal immune activation,which led to neonatal hypoxic/ischemic brain *** delive ry of hUC-MSCs inhibited PTBP-1 expression,alleviated neonatal brain injury-related inflammation,and regulated the number and function of glial fibrillary acidic protein-positive astrocytes,there by promoting plastic regeneration of neurons and im p roving brain *** findings suggest that hUC-MSCs can effectively promote the repair of neonatal hypoxic/ischemic brain injury related to maternal immune activation through inhibition of PTBP-1 expression and astrocyte activation.

关键词： developmental brain disease model disease-associated astrocytes intranasal administration lipopolysaccharide maternal immune activation neonatal brain injury neuroplasticity repair polypyrimidine tract-binding protein-1 stem cell therapy umbilical cord-derived mesenchymal stem cells

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Recent advancement of sonogenetics:A promising noninvasive cellular manipulation by ultrasound

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Genes & Diseases 2024年第5期11卷 163-182页

作者： Jin Tang Mingxuan Feng Dong Wang Liang Zhang Ke Yang Pediatric Research Institute Children’s Hospital of Chongqing Medical UniversityNational Clinical Research Center for Child Health and DisordersMinistry of Education Key Laboratory of Child Development and DisordersChina International Science and Technology Cooperation Base of Child Development and Critical DisordersChongqing Engineering Research Center of Stem Cell TherapyChongqing 400014China Department of Ultrasound The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Tongji Medical College Huazhong University of Science and TechnologyWuhanHubei 430074China

Recent advancements in biomedical research have underscored the importance of noninvasive cellular manipulation ***,a method that uses genetic engineering to produce ultrasound-sensitive proteins in target cells,is gaining prominence along with optogenetics,electrogenetics,and *** stimulation with ultrasound,these proteins trigger a cascade of cellular activities and *** traditional ultrasound modalities,sonogenetics offers enhanced spatial selectivity,improving precision and safety in disease *** technology broadens the scope of non-surgical interventions across a wide range of clinical research and therapeutic applications,including neuromodulation,oncologic treatments,stem cell therapy,and *** current literature predominantly emphasizes ultrasonic neuromodulation,this review offers a comprehensive exploration of *** discuss ultrasound properties,the specific ultrasound-sensitive proteins employed in sonogenetics,and the technique’s potential in managing conditions such as neurological disorders,cancer,and ophthalmic diseases,and in stem cell *** objective is to stimulate fresh perspectives for further research in this promising field.

关键词： Mechanosensitive channel Neuromodulation Sonogenetics Ultrasound Ultrasound-sensitive protein

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Collagen Targeting Bmscs-Derived Extracellular Vesicles Coupled with Decellularized Tendon Scaffold for Anterior Cruciate Ligament Reconstruction

SSRN

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SSRN 2024年

作者： Wang, Jie Cui, Jing Ning, Liang-Ju Zhang, Yan-Jing Xing, Fei Zhao, Lei-Lei Liu, Hui-Min Li, Xuan Zhu, Min Luo, Jing-Cong Xiang, Zhou Qin, Ting-Wu Department of Orthopedic Surgery and Orthopedic Research Institute Laboratory of Stem Cell and Tissue Engineering State Key Laboratory of Biotherapy West China Hospital Sichuan University Chengdu China

Functional reconstruction and ligament-bone integration was a current challenge in anterior cruciate ligament (ACL) reconstruction. In previous studies, decellularized tendon matrix material was considered as a promising material for tendon and ligament repair due to its easy availability, good biocompatibility and low immunogenicity. In addition, extracellular vesicles derived from bone marrow mesenchymal stem cells (BMSCS-EVs) have been repeatedly reported in recent years to play a crucial role in promoting bone regeneration and graft-bone integration, though their retention in vivo is low. This study designed a type I collagen (Col-I) targeting fusion peptide (TKKTLRTGGGGSCRHSQMTVTSRL) to engineer BMSCS-EVs. Further, the engineered BMSCS-EVs was coupled with decellularized porcine tendon sheets (DPTS) scaffold to construct a new bioactive scaffold DPTS@PEVs-DPTS-DPTS@PEVs for ACL reconstruction and its osseous integration. Our results confirmed that the bioactive scaffold improved the therapeutic concentration and duration of BMSCs-EVs in the graft-bone repair area. Moreover, the bioactive scaffold significantly promoted the adhesion, proliferation, migration and osteogenesis differentiation of BMSCs, significantly inhibit M1 but promote M2 type polarization of macrophages. In addition, the novel DPTS@PEVs-DPTS-DPTS@PEVs bioactive scaffold showed good graft-bone integration and biomechanical properties after ACL reconstruction in New Zealand white rabbits, the above advantages may provide a promising new idea for surgical reconstruction of ACL in clinic. © 2024, The Authors. All rights reserved.

关键词： stem cells

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Changing the public perception of human embryology ( vol 25, pg 1717, 2023)

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NATURE cell BIOLOGY 2023年第12期25卷 1884-1884页

作者： Rivron, Nicolas C. Martinez-Arias, Alfonso Sermon, Karen Mummery, Christine Scholer, Hans R. Wells, James Nichols, Jenny Hadjantonakis, Anna-Katerina Lancaster, Madeline A. Moris, Naomi Fu, Jianping Sturmey, Roger G. Niakan, Kathy Rossant, Janet Kato, Kazuto Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA) Vienna BioCenter (VBC) Vienna Austria Systems Bioengineering MELIS Universidad Pompeu Fabra and Catalan Institution for Research and Advanced Studies (ICREA) Barcelona Spain Research Group Reproduction and Genetics Vrije Universiteit Brussel Brussels Belgium European Society for Human Reproduction and Embryology (ESHRE) Strombeek-Bever Belgium Leiden University Medical Center Leiden the Netherlands Max Planck Institute for Molecular Biomedicine Münster Germany Center for Stem Cell and Organoid Medicine Cincinnati Children’s Hospital Medical Center Cincinnati OH USA Division of Developmental Biology Cincinnati Children’s Hospital Medical Center Cincinnati OH USA MRC Human Genetics Unit Institute of Genetics and Cancer The University of Edinburgh Crewe Road Edinburgh UK Developmental Biology Program Sloan Kettering Institute Memorial Sloan Kettering Cancer Center New York NY USA MRC Laboratory of Molecular Biology Cambridge UK The Francis Crick Institute London UK Department of Mechanical Engineering University of Michigan Ann Arbor MI USA Department of Biomedical Engineering University of Michigan Ann Arbor MI USA Department of Cell & Developmental Biology University of Michigan Medical School Ann Arbor MI USA Biomedical Institute for Multimorbidity Hull York Medical School University of Hull Hull UK Cambridge Reproduction University of Cambridge Cambridge UK The Centre for Trophoblast Research Department of Physiology Development and Neuroscience University of Cambridge Cambridge UK Human Embryo and Stem Cell Laboratory The Francis Crick Institute London UK Wellcome Trust–Medical Research Council Stem Cell Institute University of Cambridge Jeffrey Cheah Biomedical Centre Cambridge UK Epigenetics Programme Babraham Institute Cambridge UK The Hospital for Sick Children Toronto ON Canada Department of Biomedical Ethics and Public Policy Graduate School of Medicin

Human embryology is flourishing thanks to an impetus provided by embryo models formed from stem cells. These scientific advances require meticulous experimental work and a refined ethical framework, but also sensible ... 详细信息

关键词： Embryology Embryonic stem cells

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Mitochondria-specific near-infrared photoactivation of peroxynitrite upconversion luminescent nanogenerator for precision cancer gas therapy

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Acta Pharmaceutica Sinica B 2024年第1期14卷 378-391页

作者： Hui Yu Aliya Tiemuer Xufeng Yao Mingyuan Zuo Hai-Yan Wang Yi Liu Xiaoyuan Chen Department of Biomedical Engineering School of EngineeringChina Pharmaceutical UniversityNanjing 211198China School of Mechanical Engineering Southeast UniversityNanjing 211189China Departments of Diagnostic Radiology SurgeryChemical and Biomolecular Engineeringand Biomedical EngineeringYong Loo Lin School of Medicine and College of Design and EngineeringNational University of SingaporeSingapore 119074Singapore Clinical Imaging Research Centre Centre for Translational MedicineYong Loo Lin School of MedicineNational University of SingaporeSingapore 117599Singapore Nanomedicine Translational Research Program Yong Loo Lin School of MedicineNational University of SingaporeSingapore 117597Singapore Institute of Molecular and Cell Biology Agency for ScienceTechnologyand Research(A*STAR)ProteosSingapore 138673Singapore

Gas therapy is emerging as a highly promising therapeutic strategy for cancer ***,there are limitations,including the lack of targeted subcellular organelle accuracy and spatiotemporal release precision,associated with gas *** this study,we developed a series of photoactivatable nitric oxide(NO)donors NRh-R-NO(R=Me,Et,Bn,iPr,and Ph)based on an N-nitrosated upconversion luminescent rhodamine *** the irradiation of 808 nm light,only NRh-Ph-NO could effectively release NO and NRh-Ph with a significant turn-on frequency upconversion luminescence(FUCL)signal at 740 nm,ascribed to lower N-N bond dissociation *** also investigated the involved multistage near-infrared-controlled cascade release of gas therapy,including the NO released from NRh-Ph-NO along with one NRh-Ph molecule generation,the superoxide anion O_(2)^(⋅−)produced by the photodynamic therapy(PDT)effect of NRh-Ph,and highly toxic peroxynitrite anion(ONOO‒)generated from the co-existence of NO and O_(2)^(⋅−).After mild nano-modification,the nanogenerator(NRh-Ph-NO NPs)empowered with superior biocompatibility could target *** an 808 nm laser irradiation,NRh-Ph-NO NPs could induce NO/ROS to generate RNS,causing a decrease in the mitochondrial membrane potential and initiating apoptosis by caspase-3 activation,which further induced tumor immunogenic cell death(ICD).In vivo therapeutic results of NRh-Ph-NO NPs showed augmented RNS-potentiated gas therapy,demonstrating excellent biocompatibility and effective tumor inhibition guided by real-time FUCL ***,this versatile strategy defines the targeted RNS-mediated cancer therapy.

关键词： Near infrared Photo-triggered Mitochondrion Upconversion luminescent bioimaging Peroxynitrite Nanogenerator Gas therapy In vivo

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A novel strategy for treating acute liver failure:encapsulated proliferating human hepatocyte organoids

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Biomaterials Translational 2024年第4期5卷 444-446页

作者： Xiangying Meng Aihui Liu Oulayvanh Phangthavong Yi Sun Institute of Reproductive and Stem Cell Engineering School of Basic Medical ScienceCentral South UniversityChangshaHubei ProvinceChina Hunan Guangxiu Hospital Hunan Normal UniversityChangshaHubei ProvinceChina National Engineering and Research Center of Human Stem Cells ChangshaHubei ProvinceChina Key Laboratory of Stem Cells and Reproductive Engineering Ministry of HealthChangshaHubei ProvinceChina

Acute liver failure(ALF)is a short-term severe damage to hepatocytes caused by hepatotropic viruses,drug,*** accompanied by complications such as multi-organ failure and hepatic encephalopathy.1 The global incidence of ALF is estimated at over 1 million cases annually.2 Liver transplantation is currently the sole curative treatment for ALF,but due to the limitations of donor organ shortage,complications and long-term immunosuppression,hepatocytes transplantation has been proposed as an alternative strategy.3 However,the shortage of human primary liver cells(PHHs)and the difficulty in amplification in vitro hinder their clinical *** cells can provide an abundant source of cells for the treatment of ALF,but the hepatocytes derived from them have limitations in expansion,insufficient liver function and safety problems.

关键词： Acute limitations annually

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Structure-guided Redesign of OxyR-based Optogenetic Reporters for Sensitive Monitoring of Intracellular H2O2 Real-time

Structure-guided Redesign of OxyR-based Optogenetic Reporter...

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Optics and the Brain, BRAIN 2023 - Part of Optica Biophotonics Congress: Optics in the Life Sciences 2023

作者： Lee, Justin Daho Wang, Yihan Nguyen, Amanda Berndt, Andre Molecular Engineering Ph.D. Program University of Washington SeattleWA98195 United States Department of Bioengineering University of Washington SeattleWA98195 United States Institute for Stem Cell and Regenerative Medicine University of Washington SeattleWA98109 United States Department of Pharmacology Center for Neuroscience of Addiction Pain and Emotion University of Washington SeattleWA98195 United States

OxyR is a protein exhibiting exceptional sensitivity and specificity towards H2O2. However, OxyR-based optogenetic sensors do not closely recapitulate the native sensitivity and kinetics. Here, we discuss a novel desi... 详细信息

ISBN: (纸本)9781957171210

关键词： Diffusion Molecules Proteins Sensor performance Sensors Strain

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Novel Self-assembled Tellurium Nanoparticles And Their Effects In Myoblastoma cell Line

Journal of Applied Science and Engineering

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Journal of Applied Science and engineering 2025年第11期28卷 2265-2279页

作者： Chithamparam, Pandian Christyraj, Johnson Retnaraj Samuel Selvan Thiagamani, Senthil Muthu Kumar Sriariyanun, Malinee Muthukumaran, Azhaguchamy Department of Biotechnology Kalasalingam Academy of Research and Education Tamil Nadu Krishnankoil India Regeneration and Stem Cell Biology Lab Centre for Molecular and Nanomedical Sciences International Research Centre Sathyabama Institute of Science and Technology Tamil Nadu Chennai600119 India Department of Mechanical Engineering Kalasalingam Academy of Research and Education Anand Nagar Tamil Nadu Krishnankoil626126 India Department of Mechanical Engineering INTI International University Persiaran Perdana BBN Putra Nilai Negeri Sembilan Nilai71800 Malaysia UniversitiTeknologi Malaysia Skudai Johor Johor Bahru81310 Malaysia Biorefinery and Process Automation Engineering Center Department of Chemical and Process Engineering The Sirindhorn International Thai-German Graduate School of Engineering King Mongkut’s University of Technology North Bangkok Bangkok Thailand

Harnessing the distinct features of nanoparticles and nanomaterials for biomedical use has seen remarkable progress in recent years. Tellurium nanoparticles (TeNPs) have attracted attention due to their exceptional properties and potential uses in photoconductivity, nonlinear optics, piezoelectricity, and thermal conductivity applications. This study synthesized TeNPs through a novel heat reduction method using ascorbic acid as a reducing agent, followed by various characterization studies. Functional studies, such as the antibacterial activity, antioxidant activity and cytotoxicity assays, AO/EB staining, and live cell imaging were performed using TeNPs. The various concentrations of TeNPs (5 − 20 µg/ml) result in inhibition zones against *** in the 5-13 mm range and against *** in the 6-12 mm range. The produced TeNPs minimum inhibitory concentration (MIC) demonstrated that these nanoparticles had antibacterial properties against the tested gram-positive and gram-negative bacterial strains. Moreover, TeNPs exhibit strong antioxidant properties and demonstrate high toxicity in C2C12 myoblastoma cells. Notably, at a concentration of 5 µg/ml, TeNPs exhibit potential toxicity. Live cell imaging and cell proliferation assay with high and lower concentrations of TeNPs retards cell growth and proliferation of C2C12 myoblastoma cells compared to control. Based on the results, due to the increased toxicity nature of TeNPs, lower concentrations below 5 µg might be used in scaffolds to enhance cell proliferation for regenerative medicine and tissue engineering applications. © The Author(’s).

关键词： cell proliferation

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