作者：
In Sook KimJongKeun SongYun MiSongTae Hyung LeeTae Hyung ChoShin Saeng LimHui PanSung June KimSoon Jung HwangDental Research Institute
Seoul National UniversityBrain Korea 21 2nd Program for Craniomaxillofacial Life ScienceKorea School of Electrical Engineering and Computer ScienceSeoul National UniversitySeoulKorea Department of Maxillofacial Cell and Developmental BiologyDepartment of Oral and Maxillofacial SurgerySchool of DentistrySeoul National UniversityBrain Korea 21 2nd Program for Craniomaxillofacial Life ScienceSeoulKorea
Objective:Exogenous electrical stimulation has a range of biostimulatory *** work from our laboratory showed the VEGF induction and increased proliferation by biphasic electric current (BEC) in rat calvarial *** this ...
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Objective:Exogenous electrical stimulation has a range of biostimulatory *** work from our laboratory showed the VEGF induction and increased proliferation by biphasic electric current (BEC) in rat calvarial *** this study,we continued to investigate the response of human bone marrow mesenchymal
Homozygous human embryonic stemcells (hESCs) are thought to be better cell sources for hESC banking because their human leukocyte antigen (HLA) haplotype would strongly increase the degree of matching for certain pop...
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Homozygous human embryonic stemcells (hESCs) are thought to be better cell sources for hESC banking because their human leukocyte antigen (HLA) haplotype would strongly increase the degree of matching for certain populations with relatively smaller cohorts of cell lines. Homozygous hESCs can be generated from parthenogenetic embryos, but only heterozygous hESCs have been established using the current strategy to artificially activate the oocyte without second polar body extrusion. Here we report the first successful derivation of a human homozygous ESC line (chHES-32) from a one-pronuclear oocyte following routine in vitro fertilization treatment. cAHES-32 cells express common markers and genes with normal hESCs. They have been propagated in an undifferentiated state for more than a year (>P50) and have maintained a stable karyotype of 46, XX. When differentiated in vivo and in vitro, c/zHES-32 cells can form derivatives from all three embryonic germ layers. The almost undetectable expression of five paternally expressed imprinted genes and their HLA genotype identical to the oocyte donor indicated their parthenogenetic origin. Using genome-wide single-nucleotide polymorphism analysis and DNA fingerprinting, the homozygosity of c/zHES-32 cells was further confirmed. The results indicated that 'unwanted' one-pronuclear oocytes might be a potential source for human homozygous and parthenogenetic ESCs, and suggested an alternative strategy for obtaining homozygous hESC lines from parthenogenetic haploid oocytes.
Assuming homogenity of the underlying statistics, interspike interval probability density functions recorded from behaving macaque monkeys are determined with a special statistical fitting method. The nature of the pr...
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Assuming homogenity of the underlying statistics, interspike interval probability density functions recorded from behaving macaque monkeys are determined with a special statistical fitting method. The nature of the problem needed robust statistics approach, the results were validated with the Kolmogorov-Smirnov test. The results suggest that there is no single/universal interspike interval probability density function, but many, and the underlying statistics is definitely not Poissonian.
Background: Vertebral malformations contribute substantially to the pathophysiology of kyphosis and scoliosis, common health problems associated with back and neck pain, disability, cosmetic disfigurement and function...
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Microfluidic gradient generators can provide a more quantitative understanding of key developmental events. Critical to this aim is the ability to create stable, well-defined gradients without fluid flow that would ot...
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We have developed a direct conjugation method of ROD (Arg-Gly-Asp) into polylactide (PLA) using co-solvent system, which showed high reaction yield compared to one solvent system presumably due to good solubility of r...
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We have developed a direct conjugation method of ROD (Arg-Gly-Asp) into polylactide (PLA) using co-solvent system, which showed high reaction yield compared to one solvent system presumably due to good solubility of reactants. First, hydroxyl groups at the end of PLA were changed into carboxyl acid by ring opening reaction of succinic anhydride. For a coupling reaction with high solubility, ROD was dissolved in water and then PLA in dioxane was added into ROD solution in the presence of 1-(3-dimenthylaminopropyl)3-ethylcarbodiimide hydrochloride (EDC). The conjugation of ROD was confirmed by 1H-NMR, GPC and elementary analysis. The ROD conjugated PLA (RGD-PLA) on poly(lactide-co-glycolide) (PLGA) film significantly increased the number of attached cells compared to PLGA without RGD-PLA, while it did not change static contact angle of PLGA. This result demonstrated this conjugation method would be a useful tool to increase cell affinity of biomaterials without change in hydrophilic/hydrophobic property for tissue engineering applications.
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