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An eco-friendly preparation of cellulose nano crystals from oil palm empty fruit bunches

IOP Conference Series: Earth and Environmental Science 2018年第1期105卷

作者： Arie Wibowo Haroki Madani Hermawan Judawisastra Elvi Restiawaty Chrisella Lazarus Yogi W. Budhi Material Engineering Study Program Institut Teknologi Bandung Jl. Ganesha 10 Bandung Indonesia Chemical Engineering Study Program Institut Teknologi Bandung Jl. Ganesha 10 Bandung Indonesia Bioenergy and Chemurgy Engineering Institut Teknologi Bandung Jl. Ganesha 10 Bandung Indonesia

Cellulose Nanocrystals (CNCs) have attracted a lot of attention as one of the promising green nanomaterials because of their interesting properties such as low density, high mechanical properties, high surface area, high biocompatibility and low toxicity. However, sulphuric acids is generally used in conventional method to prepare CNCs which is highly corrosive and harmful to environment. Replacing sulphuric acid with Ammonium Persulfate (APS), which possess lower toxicity, are desirable from environment point of view. In this research, preparation of CNCs from Oil Palm Empty Fruit Bunches (OPEFB) as alternative raw materials and using APS is reported. Three kinds of method were carried out before applying APS solution: (i) without pre-treatment, (ii) with alkali pre-treatment and (iii) with alkali-chloride pre-treatment. White-colored sample with crystallinity reaching 78% (XRD result) and particle size ranging between 31-113 nm (DLS result) was obtained. Those results implied that there were potential existence of CNCs in the samples.

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Ferrous Ion and Medium Composition Effects on Acidogenic Phase in Biobutanol Production from Molasses

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Journal of Physics: Conference Series 2017年第1期877卷

作者： E Restiawaty D Grinanda Study Program of Bioenergy and Chemurgy Engineering Research Group of Chemical Engineering Process Design and Development Faculty of Industrial Technology Institut Teknologi Bandung Jln. Let. Jen. Purn. Dr. (HC) Mashudi No. 1 Sumedang West Java 45363 Indonesia Biosciences and Biotechnology Research Centre Institut Teknologi Bandung Jl. Ganesha 10 Bandung 40132 West Java Indonesia.

Clostridium acetobutylicum B530 has ability to convert sugar into biobutanol through two phases, i.e. acidogenic and solventogenic. This fermentation process is often hampered by high raw material cost and low product yield. In order to suppress the production cost, the molasses, a byproduct of sugar cane process production, was used as carbon source in this research. Molasses has nitrogen content in a small amount, thus could be negating the beef extract component, which is expected not to affect the growth of C. acetobutylicum B530 and also can reduce the production cost. In addition, a certain amount of Fe2+ (ferrous ion), a precursor in the formation of the enzyme ferredoxin, was added to the fermentation medium to contribute in the synthesis of acetyl-CoA, so that the formation of acidogenic products such as butyric acid and acetic acid is affected. This study aimed to investigate the effect of ferrous ion and the medium composition in acidogenic phase. The addition of 20 ppm FeSO4.7H2O in the fermentation medium without beef extract can increase the concentration of butyric acid by 20% at a temperature of 35°C, while acetic acid concentration decreased by 6%. According to those results, it is expected that the product selectivity of butanol will increase in solventogenic phase. In addition, the removal of beef extract in the fermentation medium does not affect the kinetics of growth of C. acetobutylicum B530.

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Comparison of Pretreatment Methods on Vetiver Leaves for Efficient Processes of Simultaneous Saccharification and Fermentation by Neurospora sp.

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Journal of Physics: Conference Series 2017年第1期877卷 012048-012048页

作者： Restiawaty, E Dewi, A Study Program of Bioenergy and Chemurgy Engineering Research Group of Chemical Engineering Process Design and Development Faculty of Industrial Technology Institut Teknologi Bandung Jln. Let. Jen. Purn. Dr. (HC) Mashudi No. 1 Sumedang West Java 45363 Indonesia Biosciences and Biotechnology Research Centre Institut Teknologi Bandung Jln. Ganesha 10 Bandung 40132 Indonesia Biosciences and Biotechnology Research Centre Institut Teknologi Bandung Jln. Ganesha 10 Bandung 40132 Indonesia

Lignocellulosic biomass is a potential raw material for bioethanol production. Neurospora sp. can be used to convert lignocellulosic biomass into bioethanol because of its ability to perform simultaneous saccharification and fermentation. However, lignin content, degree of polymerization, and crystallinity of cellulose contained in lignocellulosic biomass can inhibit cellulosic-biomass digestion by Neurospora sp, so that a suitable pretreatment method of lignocellulosic biomass is needed. The focus of this research was to investigate the suitable pretreatment method for vetiver leaves (Vetiveria zizanioides L. Nash) used as a raw material producing bioethanol in the process of simultaneous saccharification and fermentation (SSF) by Neurospora sp.. Vetiver plants obtained from Garut are deliberately cultivated to produce essential oils extracted from the roots of this plant. Since the vetiver leaves do not contain oil, some of harvested leaves are usually used for crafts and cattle feed, and the rest are burned. This study intended to look at other potential of vetiver leaves as a source of renewable energy. Pretreatments of the vetiver leaves were conducted using hot water, dilute acid, alkaline & dilute acid, and alkaline peroxide, in which each method was accompanied by thermal treatment. The results showed that the alkaline peroxide treatment is a suitable for vetiver leaves as indicated by the increase of cellulose content up to 65.1%, while the contents of hot water soluble, hemicellulose, lignin, and ash are 8.7%, 18.3%, 6.8%, and 1.1%, respectively. Using this pretreatment method, the vetiver leaves can be converted into bioethanol by SSF process using Neurospora sp. with a concentration of bioethanol of 6.7 g/L operated at room temperature.

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