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Protein identification and potential bioactive peptides from pumpkin (Cucurbita maxima) seeds

Food Science and Nutrition 2024年第8期12卷 5388-5402页

作者： Lin, Chu-Ti Tejano, Lhumen A. Panjaitan, Fenny Crista A. Permata, Vinny Nabila Surya Sevi, Tesalonika Chang, Yu-Wei Department of Food Science National Taiwan Ocean University Keelung Taiwan Institute of Fish Processing Technology College of Fisheries and Ocean Sciences University of the Philippines Visayas Iloilo Miagao Philippines Marine Products Processing Study Program Marine and Fisheries Polytechnic of Jembrana Bali Indonesia

Pumpkin is an economically important crop all over the world. Approximately, 18%–21% of pumpkins, consisting of peels and seeds by-products, are wasted during processing. In addition, the seeds are rich in protein and have the potency of bioactive peptide production. This study aims to recognize the proteins and investigate the potential bioactive peptides from pumpkin (Cucurbita maxima) seeds. Pumpkin seeds were subjected to hot air drying (HAD) at 55°C for 12 h and freeze-drying (FD) at −80°C for 54 h before they were powdered, analyzed, and precipitated by isoelectric point to obtain pumpkin seed protein isolates (PSPI). PSPI comprised 11S globulin subunit beta, 2S seed storage albumin, and chaperonin CPN60-1. To generate hydrolysate peptides, PSPI was hydrolyzed using papain, pepsin, and bromelain. FD group pepsin hydrolysates had the highest peptide content of 420.83 mg/g. ACE inhibition and DPP-IV inhibition activity were analyzed for each enzymatic hydrolysate. The pepsin hydrolyzed sample exhibited the highest ACE inhibition of 70.26%, and the papain hydrolyzed sample exhibited the highest DPP-IV inhibition of 30.51%. The simulated gastrointestinal digestion (SGID) conducted by pepsin and pancreatin increased ACE inhibitory activity from 76.93% to 78.34%, and DPP-IV inhibited activity increased from 58.62% to 77.13%. Pepsin and papain hydrolysates were fractionated using ultrafiltration to measure ACE and DPP-IV inhibition activity. The highest free radical scavenging abilities were exhibited by the <1 kDa hydrolysate fractions with 78.34% ACE inhibitory activities and 79.55% DPP-IV inhibitory activities. This research revealed that pumpkin seeds had the potency to produce bioactive peptides. © 2024 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC.

关键词： bioactive peptide enzymatic hydrolysis proteomics pumpkin seed

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The effect of using different polar solvents on the stability of thermal extraction phycocyanin from Spirulina platensis 2

The effect of using different polar solvents on the stabilit...

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2nd International Conference on fisheries and Marine Science, InCoFiMS 2019

作者： Irawati, D. Abdillah, A.A. Pramono, H. Sulmartiwi, L. Technology of Fisheries Processing Study Program Faculty of Fisheries and Marine Universitas Airlangga Surabaya60115 Indonesia Department of Marine Faculty of Fisheries and Marine Universitas Airlangga Surabaya60115 Indonesia

Spirulina platensis is one type of blue-green autotrophic microalgae which is included in the cyanobacteria class. Spirulina platensis microscopically, it looks like a thin thread (filament) in the form of a spiral. Phycocyanin, and phycobiliprotein are used in food, biotechnology, and the cosmetics industry because of their color and antioxidants. This goal of research is to provide information of the best polar solvents on the thermal stability of phycocyanin extract from Spirullina platensis. The research method used was the complete random design (CRD) method. The results of the concentration and purity of phycocyanin showed that the heating process using 80°C for 60 minutes produced phycocyanin. The best polar solvent showe in etanol. © Published under licence by IOP Publishing Ltd.

关键词： Microalgae

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Use of Sargassum polycystum ethanol extract as antibacterial for increasing shelf life tilapia fillet (Oreochromis niloticus) stored in chilling temperature 3

Use of Sargassum polycystum ethanol extract as antibacterial...

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3rd EMBRIO International Workshop on Marine Biodiversity: Understanding, Utilization, Conservation, EIW 2018

作者： Budi, P.H. Thaib, E.A. Julita, M. Study Program of Fisheries Processing Technology Jakarta Fisheries University Indonesia Study Program of Aquaculture Jakarta Fisheries University Indonesia

Sargassum polycystum is a seaweed that has a chemical compound that can be used as an antibacterial. Tilapia fillet is one of the fish product which is known as perishable food. The purpose of this study was to see the effect of S. polycystum ethanol extract in resisting the deterioration rate of tilapia fillet quality. The tilapia fillet was given three treatments that was soaked with 1% S. polycystum ethanol extract, positive control (washed with 10 ppm chlorine water), and negative control (water) was then stored for 10 days at a temperature of 4°C. The results of the study found that tilapia fillets that are soaked with S. polycystum ethanol extract solution can maintain the organoleptic value. TPC of tilapia fillet soaked with S. polycystum ethanol extract was better than control. Chemical test results (pH and TVB) showed the fillet that was soaked with S. polycystum ethanol extract had a better value than the control. Treatment with S. polycystum ethanol extract acts as an antibacterial compound similar to the positive control, but better than the negative control. This can be used in extending the shelf life of tilapia fillets in low temperatures by two days longer than negative control. © Published under licence by IOP Publishing Ltd.

关键词： Ethanol

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Screening marine lactic acid bacteria of brown seaweed (Sargassum polycystum) symbiotic producing bioactive compound from Indonesian waters

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AIP Conference Proceedings 2023年第1期2596卷

作者： Arma Anti Amran Laga Andi Hasizah Niken Dharmayanti Resmi Rumenta Siregar Atik Nurseha Food Science and Technology Study Program Hasanuddin University Makassar 90245 Indonesia Postgraduate Study Program on Utilization of Fisheries Jakarta Technical Fisheries University South Jakarta12520 Indonesia Fish Processing Technology Study Program Jakarta Technical Fisheries University South Jakarta 12520 Indonesia

Lactic acid bacteria can be found in a variety of environments in nature. These bacteria are well-known for their health advantages, particularly in the food and pharmaceutical industries. Terrestrial lactic acid bacteria and marine strain lactic acid bacteria have gotten greater attention for their traits and potential. The bacteria associated with brown algae (Sargassum polycystum) were identified in this investigation. The isolates were then subjected to a paper disc diffusion test to assess the potential of their bioactive compounds, which revealed that they had a higher potential for preventing the growth of gram-positive bacteria (Staphylococcus aureus). The symbiotic bacterial isolate from the algae was identified as a Lactobacillus plantarum species by phenotypic and genotypic investigation.

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Antibacterial activity of the symbiotic bacteria of green algae Caulerpa racemosa from Pulau Lima Indonesia 3

Antibacterial activity of the symbiotic bacteria of green al...

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3rd EMBRIO International Workshop on Marine Biodiversity: Understanding, Utilization, Conservation, EIW 2018

作者： Sujuliyani Thaib, E.A. Indriati, N. Liananda, M. Study Program of Fisheries Processing Technology Jakarta Fisheries University Indonesia Study Program of Aquaculture Jakarta Fisheries University Indonesia Marine and Fisheries Biotechnology and Product Processing Research Center Jakarta Indonesia

The use of symbionts is the most efficient way to obtain antibacterial bioactive compounds without the extraction of the host plant. Caulerpa racemosa or sea grapes are known to have symbiotic bacteria. This study was aimed to examine the antibacterial activity of symbiotic bacteria of C. racemosa. The research obtained two isolates from the inside of algae that formed an obstruent zone and of antagonist activity against Staphylococcus aureus and Salmonella typhi bacteria. Further analysis of the isolate coded CaD51 by paper disc diffusion showed that the bacteria produced an obstruent zone by 5.08 mm against S. aureus and had 50% Minimum Inhibitory Concertration (MIC) on both pathogenic bacteria. The bacteria reached their optimal growth rate at the 16th to 20th hour. The CaD51 were identified as gram negative bacteria, coccus, acid intolerant, not producing spores, not motile, and anaerobic facultative. Based on these key identification properties, the isolate CaD51 belongs to the genus Neisseria. © Published under licence by IOP Publishing Ltd.

关键词： Salmonella

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A potential inefficiency management: Kaizen application in the fillet processing of frozen demersal fish at PT. X Makassar-South Sulawesi 3

A potential inefficiency management: Kaizen application in t...

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3rd EMBRIO International Workshop on Marine Biodiversity: Understanding, Utilization, Conservation, EIW 2018

作者： Dharmayanti, N. Purnomo, A.H. Permadi, A. Mariyana Study Program of Fisheries Processing Technology Jakarta Fisheries University Indonesia Marine and Fisheries Biotechnology and Product Processing Research Center Jakarta Indonesia

PT. X is constantly lost profit allegedly due to inefficiencies in the processing of demersal fish fillets. This research was aimed to analyze the problem using the Kaizen method, a systematic analysis approach for continuous improvement. The research included the definition of the main problem, identification of the root causes on the employees, methods, materials and machine aspects, as well as identification of actions that are technically implementable and financially require less cost but produce maximum impact. The analysis of the production data in the frozen grouper fillets processing found differences in maximum yield in two production lines: 31.20% in Line 1 and 35.25% in Line 2, while the company's overall standard yield was 33%. These results indicated that interventions in Line 1 are needed to increase the yield by 2%. Root cause analysis found a numbery internal aspects that could be improved through Kaizen intervention, namely sorting raw materials, filleting, skinning, and trimming. Two interventions were recommended including employees training who in those aspects, and calibrating the main equipment. With such interventions, the inefficiencies worth Rp. 6.000,-/Kg of final product equivalent to Rp. 1.000.000.000,-/year of production could be overcome. © Published under licence by IOP Publishing Ltd.

关键词： Fish

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Bacillus tequilensis Isolated from Fermented Intestine of Holothuria Scabra Produces Fibrinolytic Protease with Thrombolysis Activity

Bacillus tequilensis Isolated from Fermented Intestine of Ho...

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2020 International Conference on Advances in Biological Science and technology, ICABST 2020

作者： Hidayati, Nur Nurrahman, Nurrahman Fuad, Hayatun Munandar, Hendra Zilda, Dewi Seswita Ernanto, Aditya Rahman Samiasih, Amin Oedjijono, Oedjijono Ethica, Stalis Norma Magister Study Program of Medical Laboratory Science Universitas Muhammadiyah Semarang50273 Indonesia Food Technology Study Program Faculty of Nursing and Health Sciences Universitas Muhammadiyah Semarang50273 Indonesia Lombok Nusa Tenggara Barat83352 Indonesia Research and Development Center for Marine and Fishery Product Processing and Biotechnology Ministry of Maritime Affairs and Fisheries of Indonesia Jakarta10260 Indonesia Faculty of Nursing and Health Sciences Universitas Muhammadiyah Semarang50273 Indonesia Faculty of Biology Universitas Jenderal Soedirman Purwokerto53122 Indonesia

Among essential treatment of cardiovascular disorders are fibrinolytic proteases. Most thrombolysis agents are fibrinolytic enzymes from group of bacterial proteases. This work reports a potent bacterium isolated from fermented intestine of H. scabra, which could produce fibrinolytic protease with high thrombolysis activity. Bacterial selection was conducted based on proteolytic and fibrinolytic activities indicated as clear zone on skim milk and fibrin agar media, respectively. Crude proteases from the selected bacterial isolates were subjected to thrombolytic activity test based on gravimetric method, which results were confirmed after 7 repetitions. As result, 4 fibrinolytic protease-producing bacterial isolates HFSI-3, HFSI-4, HFSI-5 and HFSI-8 were obtained. Among them, HFSI-5 isolate identified as Bacillus tequilensis on the basis of the 16S rRNA gene sequencing and morphological properties produced crude protease with the highest thrombolytic activity. The thrombolytic activity of crude protease produced by B. tequilensis HFSI-5 is worthy of comparing to that of standard fibrinolytic enzyme Nattokinase showing its potential as thrombolysis agent. © Published under licence by IOP Publishing Ltd.

关键词： Bacteriology

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Using internal transcribed spacers 2 (ITS2) to identify seaweed species from Tomini Bay and Banten Bay 3

Using internal transcribed spacers 2 (ITS2) to identify seaw...

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3rd EMBRIO International Workshop on Marine Biodiversity: Understanding, Utilization, Conservation, EIW 2018

作者： Dharmayanti, N. Abinawanto Anti, A. Study Program of Fisheries Processing Technology Jakarta Fisheries University Indonesia Study Program of Biology Mathematics and Natural Science Faculty Universitas Indonesia Jakarta Indonesia

Samples collected from two coastal areas by diving and using a boat. The fresh seaweed morphologically identified while the dried samples collected to be used for DNA testing. Previous research has been shown that there is an influence from environmental parameters on seaweed composition so that the water quality testing was carried out to determine the dominance of wild seaweed water. We conducted genomes extraction, PCR amplification, DNA sequencing testing, alignment with MEGA 7 application and made phylogeny tree, which are distributed at Tomini bay Dictyota sp., Halimeda sp., Padina sp., Polysiphonia sp., and Banten bay as Sargassum sp. This study evaluated the feasibility of using the ITS2 marker to identify some wild seaweed species, from Tomini Bay and Banten Bay waters. Five wild seaweed species collected from these waters were submitted for DNA barcoding. Currently available universal primers for ITS2 region amplification were sufficient to be successfully amplified and the result of DNA amplification continue to be sequenced. In conclusion, the data presented here indicates that ITS2 are useful markers for DNA barcoding of wild seaweed, with advantage for ITS2 due to the larger availability of analytical tools and reference barcodes deposited at databases for this marker. © Published under licence by IOP Publishing Ltd.

关键词： DNA

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The effect of using different polar solvents on the stability of thermal extraction phycocyanin from Spirulina platensis

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IOP Conference Series: Earth and Environmental Science 2020年第1期441卷

作者： D Irawati A A Abdillah H Pramono L Sulmartiwi Technology of Fisheries Processing Study program Faculty of Fisheries and Marine Universitas Airlangga Surabaya 60115 Department of Marine Faculty of Fisheries and Marine Universitas Airlangga Surabaya 60115

Spirulina platensis is one type of blue-green autotrophic microalgae which is included in the cyanobacteria class. Spirulina platensis microscopically, it looks like a thin thread (filament) in the form of a spiral. Phycocyanin, and phycobiliprotein are used in food, biotechnology, and the cosmetics industry because of their color and antioxidants. This goal of research is to provide information of the best polar solvents on the thermal stability of phycocyanin extract from Spirullina platensis. The research method used was the complete random design (CRD) method. The results of the concentration and purity of phycocyanin showed that the heating process using 80ºC for 60 minutes produced phycocyanin. The best polar solvent showe in etanol.

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Characteristics of the Encapsulated Unsaturated Fatty Acid Concentrate of Catfish Oil (Pangasius sp.)

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IOP Conference Series: Earth and Environmental Science 2021年第1期750卷

作者： E Hastarini S Z Khamidah D Fardiaz S Budijanto Research Centre for Marine Fisheries Product Processing and Biotechnology Jakarta Food Science Study Program Bogor Agricultural Institute Bogor Department of Food Science and Technology Faculty of Agricultural Technology Bogor Agricultural Institute Bogor SEAFAST Centre Bogor Agricultural Institute Bogor

Concentrated unsaturated fatty acids are processed from refined catfish oil extracted from belly meat which is the byproduct of the industry processing pangasius fillets. This belly part contains high enough fat which can be used as a source of essential fatty acids. This essential fatty acid is useful for human health and can reduce the risk of cancer and cardiovascular disease. The purpose of this study was to evaluate the physical and chemical characteristics of the encapsulated concentrated unsaturated fatty acids that have been produced. This study used a combination of coatings (maltodextrin and sodium caseinate; 9:1, 8:2, 7:3) with a ratio of unsaturated fatty acid concentrate with coating 1:5. The physicochemical characteristics analyzed were microencapsulation efficiency, dried encapsulated yield, emulsion viscosity, wettability, water solubility, morphology (SEM) and hygroscopicity. Based on the results of the study show that the ratio of 9:1 is the most optimal comparison in this study which have the lowest emulsion viscosity 12.60 cP with the efficiency of microencapsulation of 40.91% and the dried encapsulated yield of 47.82%

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