Traffic accidents often result in quick bottlenecks and increase injudicious lane changes near incidents (or lane blockage), worsening collision risks, congestion, and fuel consumption. As a practical solution, this p...
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This article presents an optimized control strategy tailored for DC islanded microgrids, featuring a voltage controller based on mixed H2/H∞ state feedback using linear matrix inequalities (LMIs) and a power controll...
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Definition of weak Pareto improvement is given for noncooperative systems with vector-valued payoff functions. The region where a system trajectory is Pareto improving is characerized. Some necessary and sufficient co...
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The paper aims to develop an innovative and robust control system for stabilizing an inverted pendulum using a neural network controller. This combines principles of control theory with the power of artificial intelli...
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Oriented ligand immobilization is one of the most effective strategies used in the design and construction of a high-capacity protein A chromatography. In this work, cysteine was introduced as anchoring sites by subst...
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Oriented ligand immobilization is one of the most effective strategies used in the design and construction of a high-capacity protein A chromatography. In this work, cysteine was introduced as anchoring sites by substituting a specific residue on Helix Ⅰ, Ⅱ, and at C-terminus of antibody binding domain Z from protein A, respectively, to investigate structural evolution and binding behavior of protein A ligands at liquid-solid interfaces. Among the three affinity dextran-coated Fe_(3)O_(4) magnetic nanoparticles(Fe_(3)O_(4)@Dx MNPs), affinity MNPs with the immobilized ligand via N11C on Helix Ⅰ(Fe_(3)O_(4)@Dx-Z_(1) MNPs) had the highest helical content, and MNPs with the immobilized ligand via G29C on Helix Ⅱ(Fe_(3)O_(4)@Dx-Z_(2) MNPs) had the lowest helical content at the same pHs. It was attributed to less electrostatic attraction of ligand to negatively charged surface on Fe_(3)O_(4)@Dx-Z_(1) MNPs because of less positive charged residues on Helix Ⅰ(K6) than Helix Ⅱ(R27/K35). Among the three affinity MNPs, moreover, the highest affinity to immunoglobulin G(IgG) binding was observed on Fe_(3)O_(4)@Dx-Z_(1) MNPs in isothermal titration calorimetry measurement, further validating greater structural integrity of the ligand on Fe_(3)O_(4)@Dx-Z_(1) MNPs. Finally,the study of IgG binding on MNPs and 96-well plates showed that anchoring sites for ligand immobilization had distinct influences on IgG binding and IgG-mediated antigen binding. This work illustrated that anchoring sites of the ligands had a striking significance for the molecular structure of the ligand at liquid-solid interfaces and raised an important implication for the design and optimization of protein A chromatography and protein A-based immunoassay analysis.
Encrypted computation opens up promising avenues across a plethora of application domains, including machine learning, health-care, finance, and control. Arithmetic homomorphic encryption, in particular, is a natural ...
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Biodiesel is gaining more interest due to its advantages over petroleum diesel, even though its production remains confronted with many challenges. These in terms of the desire for feedstocks encompass the feedstock c...
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The study investigated the enhanced production of 2-hydroxybutyric acid(2-HBA)from threonine using a two-step whole-cell bioconversion by recombinant Escherichia coli BL21(DE3)overexpressing threonine dehydratase and ...
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The study investigated the enhanced production of 2-hydroxybutyric acid(2-HBA)from threonine using a two-step whole-cell bioconversion by recombinant Escherichia coli BL21(DE3)overexpressing threonine dehydratase and *** address the rate-limiting step posed by NADH regeneration for the keto-reductase reaction converting 2-ketobutyric acid(2-KBA)to 2-HBA,formate dehydrogenase from Candida boidinii was overexpressed under the T7 promoter,resulting in a high titer of 1015 mM and a yield of 0.70 mol/***,the yield was improved by disrupting three enzymes responsible for the degradation of the intermediate(2-KBA),pyruvate-formate lyase(PflB),pyruvate oxidase(PoxB),and pyruvate dehydrogenase complex(PDHc),leading to an impressive yield of 0.99 mol/mol,closely approaching the theo-retical maximum of 1.00 mol/*** triple mutant,designed to prevent 2-KBA degradation,achieved a remarkable titer of 1,400 mM and volumetric productivity of 58 mmol/L/*** the best of our knowledge,this achievement represents the highest reported titer and yield for 2-HBA production to date.
Understanding the influencing mechanism of turbulent fluctuation on the ignition characteristics of millimeter coal particles is *** this work,to study the effect of turbulent fluctuation on ignition time,millimeter c...
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Understanding the influencing mechanism of turbulent fluctuation on the ignition characteristics of millimeter coal particles is *** this work,to study the effect of turbulent fluctuation on ignition time,millimeter coal particles are subjected to a specific flow field,generated in a furnace with symmetric fans.A one-dimensional model with the new proposed correlation and the Ranz-Marshall(R-M)correlation for Nu(Nusselt number)is established to simulate the coal ignition *** addition,the effects of fan speed,temperature,particle diameter,particle distance and coal type on the ignition time are *** is found that an increase in fan speed from 0 to 3000 rpm leads to a particle Reynolds number Re_(p)increase from 0 to 22.5,and a turbulent particle Reynolds number Re_(t)*increase from 0 to *** a consideration of the fluctuation effect,the new correlation of Nu gives a better prediction of ignition time compared to the R-M ***,the ignition time is revealed to decrease with an increasing fan speed and an elevating *** the ignition time shows merely an initial boost with enlarging particle distance,it exhibits a linearity with the term of particle diameter dp1.3-1.7 and Reynolds numbers(Nu*/Nu)-0.6(Nu*is turbulent Nusselt number).Based on this relationship,the difference of predicted ignition time is calculated at different Re_(p)and Re_(t)*.It is shown that at low Re_(p)or high Re_(t)*values,the new correlation should substitute for the R-M correlation.
In this study, a novel, robust, and optimal control algorithm is introduced for the Magnetic Levitation (MagLev) system. Initially, a model for the MagLev system that inheriting instability and featuring a non-affine ...
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