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A disordered linker in the Polycomb protein Polyhomeotic tunes phase separation and oligomerization

Molecular Cell 2025年

作者： Tim M. Gemeinhardt Roshan M. Regy Tien M. phan Nanu Pal Jyoti Sharma Olga Senkovich Andrea J. Mendiola Heather J. Ledterman Amy Henrickson daniel Lopes Utkarsh Kapoor Ashish Bihani djamouna Sihou Young C. Kim david Jeruzalmi Borries demeler Chongwoo A. Kim Jeetain Mittal Nicole J. Francis Montreal Clinical Research Institute (IRCM) Montreal QC Canada Division of Experimental Medicine McGill University Montreal QC Canada Department of Chemistry and Biochemistry The University of Lethbridge Lethbridge AB Canada Artie McFerrin Department of Chemical Engineering Texas A&M University College Station TX USA Department of Biochemistry and Molecular Medicine University of Montreal Montreal QC Canada Department of Biochemistry and Molecular Genetics Midwestern University Glendale AZ USA Department of Chemistry and Biochemistry City College of New York New York NY USA Department of Chemical and Biomedical Engineering University of Wyoming Laramie WY USA Center for Materials Physics and Technology Naval Research Laboratory Washington DC USA Ph.D. Program in Biochemistry The Graduate Center of the City University of New York New York NY USA Ph.D. Program in Biology The Graduate Center of the City University of New York New York NY USA Ph.D. Program in Chemistry The Graduate Center of the City University of New York New York NY USA Department of Chemistry and Biochemistry University of Montana Missoula MT USA Department of Chemistry Texas A&M University College Station TX USA Interdisciplinary Graduate Program in Genetics and Genomics Texas A&M University College Station TX USA

Biomolecular condensates are increasingly recognized as key regulators of chromatin organization, yet how their formation and properties arise from protein sequences remains incompletely understood. Cross-species comparisons can reveal both conserved functions and significant evolutionary differences. Here, we integrate in vitro reconstitution, molecular dynamics simulations, and cell-based assays to examine how drosophila and human variants of Polyhomeotic (ph)—a subunit of the PRC1 chromatin regulatory complex—drive condensate formation through their sterile alpha motif (SAM) oligomerization domains. We identify divergent interactions between SAM and the disordered linker connecting it to the rest of ph. These interactions enhance oligomerization and modulate both the formation and properties of reconstituted condensates. Oligomerization influences condensate dynamics but minimally impacts condensate formation. Linker-SAM interactions also affect condensate formation in drosophila and human cells and growth in drosophila imaginal discs. Our findings show how evolutionary changes in disordered linkers can fine-tune condensate properties, providing insights into sequence-function relationships.

关键词： sterile alpha motif oligomerization molecular dynamics simulations phase separation gene regulation Polycomb intrinsically disordered regions biomolecular condensates analytical ultracentrifugation biochemistry CRISPR drosophila

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Research on Key Barriers and drivers for Bicycle Industry to Implement Reverse Logistics

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Journal of Quality 2023年第6期30卷 407-442页

作者： Chang, An-Yuan Lai, Po-Yen Department of Industrial Management National Formosa University Taiwan Ph.D. Program in Smart Industry Technology Research and Design National Formosa University Taiwan

Various countries are formulating stricter laws and regulations to restrict the environmental pollution caused by various pollution. At the same time, enterprises also need to find new ways to reduce production costs. In recent years, reverse logistics (RL) have attracted the attention of enterprises and professionals, and some scholars have pointed out that if enterprises use RL technology properly, in addition to reducing operating costs, it can also gain huge benefits. Many literatures have pointed out that the implementation of RL can fully and effectively utilize resources and achieve the purpose of environmental protection. In order to implement RL smoothly, it is necessary to understand what resistance is faced and what assistance is needed. Although the implementation of RL can bring many benefits, and there are many related foreign literature studies, RL has not been popularized in the bicycle industry. Also, the discussion and research on the key barriers and key drivers are still very limited in domestic literature research. This research aims to use the multiple attribute decision making method to find out the resistance and assistance faced by Taiwan’s bicycle industry in the implementation of RL, so as to fill this knowledge gap. Through the distribution of questionnaires, experts are invited to provide data with reference to their own experience. After data integration, the fuzzy delphi method (FdM) is used to screen out important barriers and drivers, and the results are designed and distributed in a second questionnaire. Later on, the research combines with analytic hierarchy process (AHP) to get the key barriers. Finally, technique for order preference by similarity to ideal solution (TOPSIS) is used to obtain the key drivers, and the key factors are sorted in sequence. © 2023, Chinese Society for Quality. All rights reserved.

关键词： Operating costs

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Application of a Self-Assembly Sensing Layer to Grating Sensor Cabinets for Biomedical Sensing of Rheumatoid Arthritis Antigen-Antibodies

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IEEE Sensors Journal 2024年第7期24卷 10039-10047页

作者： Wen, Hsin-Yi Hsu, Hsiang-Cheng Feng, Wen-Kai Jhan, Kai-Siang Yeh, Yao-Tsung Chiang, Chia-Chin National Kaohsiung University of Science and Technology Department of Chemical and Materials Engineering Kaohsiung80778 Taiwan National Kaohsiung University of Science and Technology College of Maritime Department of Ph.D. Program in Maritime Science and Technology Kaohsiung807618 Taiwan National Kaohsiung University of Science and Technology Department of Mechanical Engineering Kaohsiung80778 Taiwan Fooyin University Department of Medical Laboratory Science and Biotechnology Kaohsiung83102 Taiwan

In this article, we harnessed biomedical applications in laser-assisted etching long-period fiber gratings (LLPFGs) sensors, subsequently deploying them within the realm of biomedical applications. The primary aim of this study was to devise a sensitive method for the detection of antigen-antibodies associated with rheumatoid arthritis (RA) using LLPFGs. Sensing the antigen binding took place on the surface of the optical fiber through a self-assembling process. Given the inherent limitations of conventional rheumatoid factor (RF) testing in terms of specificity, we introduced an innovative serum-based approach geared toward the identification of anti-cyclic citrullinated peptide (Anti-CCP) antibodies. This method exhibited an impressive diagnostic accuracy rate of approximately 80% for RA cases, coupled with an exceptional specificity level of 98% for individuals without RA. The incorporation of LLPFGs in the detection of RA antigen-antibodies presents a highly promising avenue for enhancing the precision and reliability of RA diagnosis. Furthermore, this research aligns closely with the United Nations Sustainable development Goal (SdG)3, which strives to promote well-being across all age groups and advance sustainable development by addressing health-related challenges, including the accurate diagnosis of diseases like RA. © 2023 IEEE.

关键词： Antibodies

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Engineering H2O2 Self-Supplying Platform for Xdynamic Therapies via Ru-Cu Peroxide Nanocarrier: Tumor Microenvironment-Mediated Synergistic Therapy

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ACS Applied Materials and Interfaces 2024年第19期16卷 24172-24190页

作者： dirersa, Worku Batu Kan, Tzu-Chun Chang, Jungshan Getachew, Girum Ochirbat, Sonjid Kizhepat, Shamsa Wibrianto, Aswandi Rasal, Akash Chen, Hung-An Ghule, Anil Vithal Chou, Tzung-Han Chang, Jia-Yaw Department of Chemical Engineering National Taiwan University of Science and Technology Taipei106335 Taiwan Graduate Institute of Medical Sciences College of Medicine Taipei Medical University Taipei11031 Taiwan International Master/Ph.D. Program in Medicine College of Medicine Taipei Medical University Taipei11031 Taiwan International Ph.D. Program for Cell Therapy and Regeneration Medicine College of Medicine Taipei Medical University Taipei11031 Taiwan Green Nanotechnology Laboratory Department of Chemistry Shivaji University Kolhapur416004 India Department of Chemical and Materials Engineering National Yunlin University of Science and Technology Yunlin64002 Taiwan

Of the most common, hypoxia, overexpressed glutathione (GSH), and insufficient H2O2 concentration in the tumor microenvironment (TME) are the main barriers to the advancment of reactive oxygen species (ROS) mediated Xdynamic therapies (X = photo, chemodynamic, chemo). Maximizing Fenton catalytic efficiency is crucial in chemodynamic therapy (CdT), yet endogenous H2O2 levels are not sufficient to attain better anticancer efficacy. Specifically, there is a need to amplify Fenton reactivity within tumors, leveraging the unique attributes of the TME. Herein, for the first time, we design RuxCu1-xO2-Ce6/CPT (RCpCCPT) anticancer nanoagent for TME-mediated synergistic therapy based on heterogeneous Ru-Cu peroxide nanodots (RuxCu1-xO2 Nds) and chlorine e6 (Ce6), loaded with ROS-responsive thioketal (TK) linked-camptothecin (CPT). The Ru-Cu peroxide Nds (RCp Nds, x = 0.50) possess the highest oxygen vacancy (OV) density, which grants them the potential to form massive Lewis’s acid sites for peroxide adsorption, while the dispersibility and targetability of the Nds were improved via surface modification using hyaluronic acid (HA). In TME, RCpCCPT degrades, releasing H2O2, Ru2+/3+, and Cu+/2+ ions, which cooperatively facilitate hydroxyl radical (•OH) formation and deactivate antioxidant GSH enzymes through a cocatalytic loop, resulting in excellent tumor therapeutic efficacy. Furthermore, when combined with laser treatment, RCpCCPT produces singlet oxygen (1O2) for PdT, which induces cell apoptosis at tumor sites. Following ROS generation, the TK linkage is disrupted, releasing up to 92% of the CPT within 48 h. In vitro investigations showed that laser-treated RCpCCPT caused 81.5% cell death from PdT/CdT and chemotherapy (CT). RCpCCPT in cancer cells produces red-blue emission in images of cells taking them in, which allows for fluorescence image-guided Xdynamic treatment. The overall results show that RCp Nds and RCpCCPT are more biocompatible and have excellent Xdynamic the

关键词： Oxidation

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Visual Field Examination technology Based on Semi-transparent Elliptical Mirror 6

Visual Field Examination Technology Based on Semi-transparen...

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6th IEEE Eurasian Conference on Educational Innovation, ECEI 2023

作者： Chang, Chao-Kai Chiang, Chun-Yu Yeh, Feng-Ming Lee, Chi-Hung Tien, Chuen-Lin Chen, der-Chin Department of Optometry Yuanpei University of Medical Technology Hsinchu Taiwan Ph.D. Program of Electrical and Communications Engineering Feng Chia University Taichung Taiwan Department of Electrical Engineering Feng Chia University Taichung Taiwan

ISBN: (纸本)9798350334289

In this study, the human visual field test technology is established by using an optoelectrical system consisting of a translucent ellipsoid, an area light source, and a landscape scene with an indicator. during the test, the subjects stared at the indicator directly in front of them with one eye and kept their eyeballs still, scanning the light strip with peripheral vision and using the mouse button plus the playback program to record the start and end positions of the scanning light strip in the landscape scene. This method is tested subjectively, and the subject needs to respond during the test. Therefore, before the test, the subject needs to understand the test process and be trained in advance to improve the exam accuracy. In the experiment, fifteen subjects with normal vision and no eye disease were selected. Their visual fields were confirmed normal after a preliminary assessment. The test results showed that the subjects' eyes were within the normal visual field, indicating that this method was feasible. In this research, a digital optical platform for the near visual field is proposed to establish an optoelectric visual field measurement technology. The proposed method is characterized by small size, rapid operation, and easy portability. With the digital LCd and visual imaging technology, both dynamic and static visual fields can be tested. The test data on the eyes' upper, lower, nasal, and temporal sides were consistent with the normal visual field. This shows that this method is feasible and worthy of promotion in the optometry industry. © 2023 IEEE.

关键词： Light sources

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Rising threat of metallo-β-lactamase-producing Klebsiella oxytoca complex in Taiwan, 2013–2022

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International Journal of Antimicrobial Agents 2025年第2期66卷 107515-107515页

作者： Huang, Yu-Tsung Bregente, Carl Jay Ballena Hsu, Wei-Yu Liao, Chun-Hsing Kuo, Yao-Wen Lee, Jia-Arng Lee, Tai-fen Kao, Cheng-Yen Hsueh, Po-Ren Department of Laboratory Medicine National Taiwan University Hospital National Taiwan University College of Medicine Taipei Taiwan College of Medical Technology Southwestern University PHINMA Cebu Philippines Institute of Microbiology and Immunology College of Life Sciences National Yang Ming Chiao Tung University Taipei Taiwan Department of Internal Medicine Far Eastern Memorial Hospital New Taipei City Taiwan Department of Internal Medicine National Taiwan University Hospital National Taiwan University College of Medicine Taipei Taiwan Microbiota Research Center National Yang Ming Chiao Tung University Taipei Taiwan Departments of Laboratory Medicine and Internal Medicine China Medical University Hospital China Medical University Taichung Taiwan Ph.D. Program for Aging College of Medicine China Medical University Taichung Taiwan

Objectives: The emergence of metallo-beta-lactamases (MBLs) within the Klebsiella oxytoca complex (CRKO) poses a significant challenge in Taiwan, with so far incomplete characterization of species distribution and carbapenemase variants. This study aimed to elucidate the diversity, variants, and clinical presentations of CRKO isolates collected from 2013 to 2022. Methods: We analyzed production of carbapenemases by the modified carbapenem inhibitory method (mCIM) and the NG test^TM CARBA-5. Confirmation of five common carbapenemases and multilocus sequence typing were determined by polymerase chain reaction and sequencing. Nanopore whole-genome sequencing was used for the species differentiation and plasmids analysis. We also evaluated the clinical characteristics of the CRKO-infected patients. Results: Three species were identified: K. michiganensis (n = 102), K. pasteurii (n = 13), and K. oxytoca (n = 3). A majority of isolates (91.5%, n = 108) harbored at least one MBL gene, including bla_VIM-1 (n = 56), bla_IMP-8 (n = 26), and bla_NdM-1 (n = 19), with six isolates carrying dual carbapenemase genes. The bla_NdM-1 and bla_IMP-8 genes were located on IncFII(Yp) and IncA/C2 plasmids, respectively. Notably, 34.7% of isolates carried kleboxymycin gene clusters (KGC). Using CLSI and EUCAST criteria, 92.4% and 82.4% of isolates, respectively, were susceptible to cefiderocol. Among 92 infected patients, the 30-day mortality rate was 21.7%, associated with female gender, higher Charlson Comorbidity Index, higher Pitt bacteremia scores, presence of bacteremia, and inversely associated with KGC. Conclusion: The high prevalence of CRKO carrying MBLs and the recent surge of the ST27-bla_NdM-1 genotype, poses significant treatment challenges and underscores the urgent need for enhanced surveillance in Taiwan. © 2025 Elsevier Ltd and International Society of Antimicrobial Chemotherapy

关键词： Carbapenem-resistant Cefiderocol Klebsiella oxytoca complex Metallo-β-lactamase

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Analysis on Predictive Water Temperature with PId Controller and SCAdA System

Analysis on Predictive Water Temperature with PID Controller...

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2021 International Symposium on Intelligent Signal Processing and Communication Systems, ISPACS 2021

作者： Tu, Wen-Fu Wang, Yi-Shun Chen, Tu-Sheng National Kaohsiung University of Science and Technology Marit Mechatronics Engineering Lab Department of Marine Engineering Kaohsiung Taiwan National Kaohsiung University of Science and Technology Ph.D. Program In Maritime Science and Technology College of Maritime Kaohsiung Taiwan

ISBN: (纸本)9781665419512

Marine diesel engine start-up goes through the following speed stages: dead slow, slow, half, and full. The jacket water should be heated initially to prevent the cylinder from cracking due to excessive temperature difference. In this paper, PId temperature controller and SCAdA system are used to heat the jacket water. Experimental tests have been evaluated to improve closed loop response with PId temperature controller and SCAdA system. The heating mode calls the appropriate PId controller according to the design temperature to compare the actual temperature at different stages. The experimental results show that the PId controller converges the temperature well between 35and 80, the optimal PId parameter are P=3, I=198, d=495 with the auto tune function and the max. power supply current I=8.8A, voltage V=110V. © 2021 IEEE.

关键词： Three term control systems

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deep Learning-Based Positioning Error Fault diagnosis of Wire Bonding Equipment and an Empirical Study for IC Packaging

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IEEE Transactions on Semiconductor Manufacturing 2023年第4期36卷 619-628页

作者： Kao, Sheng-Xiang Chien, Chen-Fu International Intercollegiate Ph.D. Program National Tsing Hua University Hsinchu30013 Taiwan Intelligent Manufacturing and Circular Economy Research Center National Tsing Hua University Artificial Intelligence for Intelligent Manufacturing Systems Research Center National Science and Technology Council Department of Industrial Engineering and Engineering Management Hsinchu300044 Taiwan

Little research has been done for artificial intelligence applications of semiconductor backend. This study aims to develop a deep learning based fault diagnosis framework as prognostics and health management (phM) solutions with a comprehensive analytics process. A linear encoder sensor is employed to measure position, while data preparation is conducted to convert position information into a signal. Then, signal processing is used to extract the features, in which high pass filter is applied to normalize the signal and emphasize the features. High-dimensional features including time domain statistical features, time-frequency domain features obtained by continuous wavelet transform (CWT), and frequency domain features converted by fast Fourier transform (FFT) are extracted to prepare the dataset. An ensemble neural network model that integrates deep neural network (dNN) and convolutional neural network (CNN) is employed to recognize the pattern and diagnose the positioning errors. The accuracy and false alarm rate are considered to support maintenance decisions. An empirical study was conducted in a world leading IC assembly and testing company for validation. The results have shown that the proposed approach can effectively detect inappropriate installation of the bond head in wire bonding equipment for predictive maintenance and cost reduction. © 1988-2012 IEEE.

关键词： Failure analysis

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Overcoming the Blood-Brain Tumor Barrier with docetaxel-Loaded Mesoporous Silica Nanoparticles for Treatment of Temozolomide-Resistant Glioblastoma

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ACS Applied Materials and Interfaces 2024年第17期16卷 21722-21735页

作者： Hsu, Tsung-I Chen, Yi-Ping Zhang, Rong-Lin Chen, Zih-An Wu, Cheng-Hsun Chang, Wen-Chang Mou, Chung-Yuan Chan, Hardy Wai-Hong Wu, Si-Han Ph.D. Program in Medical Neuroscience College of Medical Science and Technology Taipei Medical University and National Health Research Institutes Taipei110 Taiwan International Master Program in Medical Neuroscience College of Medical Science and Technology Taipei Medical University Taipei110 Taiwan Graduate Institute of Nanomedicine and Medical Engineering Taipei Medical University Taipei110 Taiwan International Ph.D. Program in Biomedical Engineering College of Biomedical Engineering Taipei Medical University Taipei110 Taiwan Nano Targeting & Therapy Biopharma Inc. Taipei110 Taiwan Graduate Institute of Medical Sciences College of Medicine Taipei Medical University Taipei110 Taiwan Department of Chemistry National Taiwan University Taipei106 Taiwan

While temozolomide (TMZ) has been a cornerstone in the treatment of newly diagnosed glioblastoma (GBM), a significant challenge has been the emergence of resistance to TMZ, which compromises its clinical benefits. Additionally, the nonspecificity of TMZ can lead to detrimental side effects. Although TMZ is capable of penetrating the blood-brain barrier (BBB), our research addresses the need for targeted therapy to circumvent resistance mechanisms and reduce off-target effects. This study introduces the use of PEGylated mesoporous silica nanoparticles (MSN) with octyl group modifications (C8-MSN) as a nanocarrier system for the delivery of docetaxel (dTX), providing a novel approach for treating TMZ-resistant GBM. Our findings reveal that C8-MSN is biocompatible in vitro, and dTX@C8-MSN shows no hemolytic activity at therapeutic concentrations, maintaining efficacy against GBM cells. Crucially, in vivo imaging demonstrates preferential accumulation of C8-MSN within the tumor region, suggesting enhanced permeability across the blood-brain tumor barrier (BBTB). When administered to orthotopic glioma mouse models, dTX@C8-MSN notably prolongs survival by over 50%, significantly reduces tumor volume, and decreases side effects compared to free dTX, indicating a targeted and effective approach to treatment. The apoptotic pathways activated by dTX@C8-MSN, evidenced by the increased levels of cleaved caspase-3 and PARP, point to a potent therapeutic mechanism. Collectively, the results advocate dTX@C8-MSN as a promising candidate for targeted therapy in TMZ-resistant GBM, optimizing drug delivery and bioavailability to overcome current therapeutic limitations. © 2024 The Authors. Published by American Chemical Society.

关键词： Tumors

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Properties of the mixing layer height retrieved from ceilometer measurements in Slovakia and its relationship to the air pollutant concentrations

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Environmental Science and Pollution Research 2023年第54期30卷 115666-115682页

作者： Nguyen, duy-Hieu Štefánik, dušan Šedivá, Tereza Lin, Chitsan Ph.D. Program in Maritime Science and Technology College of Maritime National Kaohsiung University of Science and Technology Kaohsiung811213 Taiwan Slovak Hydrometeorological Institute Jeséniova 17 Bratislava833 15 Slovakia Faculty of Mathematics Physics and Informatics Comenius University in Bratislava Mlynská Dolina F1 Bratislava842 48 Slovakia Department of Marine Environmental Engineering National Kaohsiung University of Science and Technology Kaohsiung811213 Taiwan

Mixing layer height (MLH) is an important meteorological parameter for air quality since it significantly affects ground-level pollution in the atmosphere. This study examined the properties of the MLH on diurnal and seasonal timescales over a 3-year period (2020–2022) using high temporal resolution measurements from eight Vaisala CL31 ceilometers situated around Slovakia. Hourly averaged MLH data was retrieved from the BL-View software using merged method. The highest daily maxima for the MLH occurred mostly in summer and spring, while the lowest values occurred predominantly during winter and autumn. The average MLH daily maximum in summer was 2229 m, and just 859 m in winter. during summer, the spatial distribution of the MLH daily maxima was more uniform compared to winter, when the air masses within the individual valleys did not mix well. Correlations between ground-level pollutant concentrations and hourly mean/daily mean MLH were analyzed. The highest correlation, R≈0.6, was found for O3. For PM10, PM2.5, and NOx, the anticorrelations with MLH were found with maximum in winter (R ≈ − 0.3 for hourly data and R ≈ − 0.5 for daily mean data) but no relation in summer. Lastly, the ceilometer MLH was compared to the radiosonde retrieved MLH for various cloud covers. Our analysis is based on an extensive set of empirical data, which can improve the accuracy and effectiveness of meteorological and atmospheric chemistry models. The findings can support air pollution forecasting and warning systems, providing valuable insights for policymakers and researchers. © 2023, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

关键词： Air quality

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