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A time-coded multi-concentration microfluidic chemical waveform generator for high-throughput probing suspension single-cell signaling

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Chinese Chemical Letters 2022年第6期33卷 3091-3096页

作者： Yiran Guo Peng Chen Zhaolong Gao Yiwei Li Shunji Li Xiaojun Feng Bi-Feng Liu The Key Laboratory for Biomedical Photonics of MOE at Wuhan National Laboratory for Optoelectronics-Hubei Bioinformatics and Molecular Imaging Key Laboratory Systems Biology ThemeDepartment of Biomedical EngineeringCollege of Life Science and TechnologyHuazhong University of Science and TechnologyWuhan 430074China The Key Laboratory of Ministry of Education for Image Processing and Intelligent Control School of Artificial Intelligence and AutomationHuazhong University of Science and TechnologyWuhan 430074China

The cellular response to the complex extracellular microenvironment is highly dynamic in time and type of extracellular *** reconstructing this process and analyzing the changes in receptor conformation on the cell membrane surface and intracellular or intercellular signaling has been a major challenge in analytical chemistry and biophysical *** this paper,a time-coded multiconcentration microfluidic chemical waveform generator was developed for the dynamic signaling probing with single-cell array of high temporal resolution,high throughput,and multi-concentration combination *** on innovative microchannel structure,sophisticated external control methods and multiplexing technology,the system not only allowed for temporally sequential permutations of the four concentrations of stimuli(time code),but also generated pulsed and continuous waveforms at different frequencies in a highly controllable ***,the single-cell trap array was set up to efficiently capture cells in suspension,dramatically increasing throughput and reducing experiment preparation *** maximum frequency of the platform was 1 Hz,and one cell could be stimulated at multiple *** show the ability of the system to investigate rapid biochemical events in high throughput,pulse stimulation and continuous stimulation of different frequencies and different time codes,combined with four concentrations of histamine(HA),were generated for probing G protein-coupled receptor(GPCR)signaling in He La ***,statistical analysis was performed for the mean peak height and mean peak area of the cellular *** believe that the time-coded multi-concentration microfluidic chemical waveform generator will provide a novel strategy for analytical chemistry,biophysics,cell signaling,and individualized medicine applications.

关键词： Microfluidics Dynamic stimulation Time code High-throughput Single-cell array

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Echo state Networks based method for harmonic extraction in Shunt Active Power Filters

Echo State Networks based method for harmonic extraction in ...

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6th International Conference on Bio-Inspired Computing: Theories and Applications, BIC-TA 2011

作者： Xu, Jinbang Yang, Jun Liu, Feng Zhang, Zhixiong Shen, Anwen Dep. of Control Sci. and Tech. Huazhong University of Sci. and Tech. Image Processing and Intelligent Control Key Laboratory of Education Ministry of China Wuhan China

ISBN: (纸本)9780769545141

With the wide use of power conversion devices, harmonic currents are being injected into the power grid. Shunt Active Power Filters (SAPF) is a power electronic device to compensate the harmonic currents caused by nonlinear loads. As the foundation of the harmonics recognition and compensation, harmonic extraction techniques are becoming more and more important. This paper proposes a new harmonic extraction method based on the Echo state Networks (ESN). ESN is a new type of Recurrent Neural Networks (RNN), which has much faster training speed than other types of RNN. To evaluate the dynamic system modeling capability of the ESN, the ESN with different dynamic reservoir size are discussed. The performance of the ESN based harmonic extraction method is compared with traditional methods and method based on multilayer perceptron networks (MLP). The ESN algorithm is trained and tested in MATLAB. © 2011 IEEE.

关键词： Recurrent neural networks

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The application of support vector machines and improved particle swarm optimization

The application of support vector machines and improved part...

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2012 International Conference on Modelling,Identification and control(建模、辨别与控制国际会议 ICMIC 2012)

作者： Hu Zhang Min Wang Xinhan Huang Key Laboratory of Image Processing and Intelligent Control of Education Department of Control Scienc Key Laboratory of Image Processing and IntelligentControl of Education Department of Control Science Key Laboratory of Image Processing andIntelligent Control of Education Department of Control Science

ISBN: (纸本)9781467315241

Parameters selection of support vector machine is the key issue that impacts its accurate performance.A method for support vector regression machine with standard particle swarm optimization (SPSO) algorithm is proposed in this ***, in order to improve the performance of the SPSO algorithm, the concept of the particles’ average distance and fitness variance is proposed to make the efficiency of algorithm ***, the improve algorithm was also applied in this *** two different models using SPSO and IPSO respectively were used to forecast the density of the acid-lead battery *** experimental results indicate that both SPSO and IPSO have high prediction accuracy and *** time of the parametric searching by IPSO is obviously decreased to that of *** mean squared error (MSE) of the prediction model using SPSO is about 2.02056×10-4, Meanwhile, the MSE of the model using IPSO is only about 1.9324×***, the IPSO algorithm has more superior performance on convergence speed and global optimization.

关键词： support vector regression particle swarm optimization average particle distance fitness variance

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A Novel Small Dim Infrared Target Detecting Method based on Grayscale Merging and Connected Components Analysis

A Novel Small Dim Infrared Target Detecting Method based on ...

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2012 International Conference on Modelling,Identification and control(建模、辨别与控制国际会议 ICMIC 2012)

作者： Ying Zhang Min Wang Shaoqing Niu Key Laboratory of Image Processing and Intelligent Control of Education Department of Control Scienc Key Laboratory of Image Processing and IntelligentControl of Education Department of Control Science Key Laboratory of Image Processing andIntelligent Control of Education Department of Control Science

Target detecting algorithm in infrared image is drawing extensive attention both at home and abroad, expecially when the infrared images own complex backgrounds and low *** to make sure of the accuracy of infrared dim-small target detecting is the key point during the processing *** this paper, a novel region of interest detecting algorithm is proposed for aerial small dim infrared target with clutter *** from traditional region of interest extraction method, we extract region of interest based on the grayscale merging algorithm and connected components analysising ***, the infrared image is reconstructed by separating the grayscale into several interval sections, then binarization is used to segment the processed image according to estimate the threshold value using minimum error probability;finally, analysis and select the connected components, to sift the accurate connected domain which contain the *** validity of the proposed approach is demonstrated by experiment of infrared target region extraction.

关键词： Infrared small-dim target object detection connected component minimum error probability

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Computer simulation for medical ultrasound c-mode imaging based on 2d array

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2012 2nd International Conference on Materials Science and Information Technology, MSIT 2012

作者： Hua, Shaoyan Yuchi, Ming Ding, Mingyue Key Laboratory of Image Processing and Intelligent Control of Education Ministry of China Biomedical Engineering Department School of life Science and Technology Huazhong University of Science and Technology Wuhan China

ISBN: (纸本)9783037854389

C-mode imaging is one of the ultrasound imaging modalities. Compared with other modalities, e.g. A-mode, B-mode, M-mode, and Doppler, C-mode is mainly developed and used in industry testing. The potential of C-mode imaging for medical application has not been fully explored. In this paper, we design one 2-d plane array transducer through using the point spread function (PSF), and apply the 2d array transducer for C-mode imaging. The simulation results show that the generated C-mode images can display the anatomic structure and the pathological changes on the biological tissue. © (2012) Trans Tech Publications, Switzerland.

关键词： Optical transfer function

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Facial Expression Recognition In-the-Wild with Deep Pre-trained Models 17th

Facial Expression Recognition In-the-Wild with Deep Pre-tra...

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17th European Conference on Computer Vision, ECCV 2022

作者： Li, Siyang Xu, Yifan Wu, Huanyu Wu, Dongrui Yin, Yingjie Cao, Jiajiong Ding, Jingting Ministry of Education Key Laboratory of Image Processing and Intelligent Control School of Artificial Intelligence and Automation Huazhong University of Science and Technology Wuhan China Ant Group Hangzhou China

ISBN: (纸本)9783031250743

Facial expression recognition (FER) is challenging, when transiting from the laboratory to in-the-wild situations. In this paper, we present a general framework for the Learning from Synthetic Data Challenge in the 4th Affective Behavior Analysis In-The-Wild (ABAW4) competition, to learn as much knowledge as possible from synthetic faces with expressions. To cope with four problems in training robust deep FER models, including uncertain labels, class imbalance, mismatch between pretraining and downstream tasks, and incapability of a single model structure, our framework consists of four respective modules, which can be utilized for FER in-the-wild. Experimental results on the official validation set from the competition demonstrated that our proposed approach outperformed the baseline by a large margin. © 2023, The Author(s), under exclusive license to Springer Nature Switzerland AG.

关键词： Face recognition

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A double-integrator system for distributed optimization of convex cost functions 29

A double-integrator system for distributed optimization of c...

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第29届中国控制与决策会议

作者： Ngoc-Tu Tran Xuan Feng Wen-Yuan Xu Na Zhan Xi Wang School of Automation Huazhong University of Science and Technology Key Laboratory of Image Processing and Intelligent Control(Huazhong University of Science and Technology) Ministry of Education Hubei Electric Power Company

ISBN: (纸本)9781509046584

In this paper,the distributed optimization problem is investigated under a second-order multi-agent *** the proposed algorithm,each agent solves the optimization via local computation and information exchange with its neighbors through the communication ***,in comparison with the existing second-order distributed optimization algorithms,the proposed algorithm is much simpler due to one coupled information exchange among the agents is *** achieve the optimization,the distributed algorithm is proposed based on the consensus method and the gradient *** optimal solution of the problem is thus obtained with the design of Lyapunov function and the help of LaSallel's Invariance Principle.A numerical simulation example and comparison of proposed algorithm with existing works are presented to illustrate the effectiveness of the theoretical result.

关键词： Second-order multi-agent systems Distributed optimization Consensus Lyapunov function Gradient algorithm

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An Online Ascent Phase Trajectory Reconstruction Algorithm Using Gauss Pseudospectral Method

An Online Ascent Phase Trajectory Reconstruction Algorithm U...

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2012 International Conference on Modelling,Identification and control(建模、辨别与控制国际会议 ICMIC 2012)

作者： Da Zhang Xuefang Lu YongJi Wang Lei Liu Key Laboratory of Ministry of Education for Image Processing and Intelligent Control Department of C Key Laboratory of Ministry of Education for Image Processing and Intelligent ControlDepartment of Co

ISBN: (纸本)9781467315241

In this paper, we present an online ascent phase trajectory reconstruction algorithm using Gauss Pseudospectral *** Pseudospectral Method (GPM) was used to transform the ascent phase trajectory optimization problem into Nonlinear Program Problem (NLP), we solve the problem using a optimization software named GPOPS in *** this method we can calculate the optimal trajectory control volume and feedback to update the guidance commands *** analysis of the optimized results has been done to prove its *** last, we compare the trajectory recon- struction algorithm to a law without reconstruction algorithm via simulation with aerodynamic and thrust *** simulation results indicate that the method proposed above offers improved performance and has ability for real-time calculating.

关键词： Ascent Phase Trajectory Online Trajectory Reconstruction Gauss Pseudospectral Method Nonlinear Pro- gramming

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An overview of Hierarchical Temporal Memory: A new neocortex algorithm

An overview of Hierarchical Temporal Memory: A new neocortex...

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2012 International Conference on Modelling, Identification and control, ICMIC 2012

作者： Chen, Xi Wang, Wei Li, Wei Institute of System Engineering Huazhong University of Science and Technology Education Ministry of China Wuhan Hubei 430074 China Department of Control Science and Engineering Image Processing and Intelligent Control Key Laboratory Education Ministry of China China

ISBN: (纸本)9780956715715

The overview presents the development and application of Hierarchical Temporal Memory (HTM). HTM is a new machine learning method which was proposed by Jeff Hawkins in 2005. It is a biologically inspired cognitive method based on the principle of how human brain works. The method invites hierarchical structure and proposes a memory-prediction framework, thus making it able to predict what will happen in the near future. This overview mainly introduces the developing process of HTM, as well as its principle, characteristics, advantages and applications in vision, image processing and robots movement, some potential applications by using HTM, such as thinking process, are also put forward. © 2012 Huazhong Univ of Sci & Tec.

关键词： Forecasting

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Analysis of memory property on the memristor based on the current and constant of integration

Analysis of memory property on the memristor based on the cu...

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2011 International Conference on Information Science and Technology, ICIST 2011

作者： Bao, Gang Shen, Yi Zeng, Zhigang Department of Control Science and Engineering Huazhong University of Science and Technology Wuhan Hubei 430074 China Key Laboratory of Image Processing and Intelligent Control Education Ministry of China Wuhan Hubei 430074 China

ISBN: (纸本)9781424494408

Memristor has received significant attentions since Strukov et al released their invention on April 30, 2008 at Nature Letters. Based on the model of Strukov et al, analytic expression of the internal state is obtained by applying voltage to the device. By using the constant in the expression of the internal state, memory property of the memristor is explained. © 2011 IEEE.

关键词： Memristors

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