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作者： Waldeisen, John R. Wang, Tim Ross, Benjamin M. Lee, Luke P. UC Berkeley/UCSF Graduate Program in Bioengineering Biomolecular Nanotechnology Center University of California Berkeley CA 94720 United States Department of Bioengineering Biomolecular Nanotechnology Center University of California Berkeley CA 94720 United States

The disassembly of a core-satellite nanostructured substrate is presented as a colorimetric biosensor observable under dark-field illumination. The fabrication method described herein utilizes thiol-mediated adsorption and streptavidin-biotin binding to self-assemble core-satellite nanostructures with a sacrificial linking peptide. Biosensing functionality is demonstrated with the protease trypsin, and the optical properties of the nanoassemblies are characterized. A figure of merit is presented to determine the optimal core and satellite size for visual detection. Nanoassemblies with 50 nm cores and 30 or 50 nm satellites are superior as these structures achieve an orange to green color shift greater than 70 nm that is easily discernible by the naked eye. This colorimetric substrate may prove to be a favorable alternative to liquid-based colloidal sensors and a useful visual readout mechanism for point-of-care microfluidic diagnostic assays. © 2011 American Chemical Society.

关键词： Biosensors

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SAME-CELL, SINGLE-CELL DETECTION OF PROTEIN ISOFORMS AND NucLEIC ACIDS 25

SAME-CELL, SINGLE-CELL DETECTION OF PROTEIN ISOFORMS AND NUC...

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25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021

作者： Gomez Martinez, Ana E. Rosas-Canyelles, Elisabet Modzelewski, Andrew J. Geldert, Alisha Gopal, Anjali He, Lin Herr, Amy E. Department of Bioengineering University of California Berkeley BerkeleyCA United States UC Berkeley-UCSF Graduate Program in Bioengineering United States Division of Cellular and Developmental Biology Department of Molecular & Cell Biology University of California BerkeleyCA United States

ISBN: (纸本)9781733419031

Single-cell 'omics is advancing rapidly, but there are gaps in linking nucleic acids to proteins in their myriad forms. To surmount this gap, we describe a microfluidic device that reports information on nucleic acids and proteoforms, or proteins that arise from a variety of modifications to nucleic acids or proteins, which are detected by immunoblotting (triBlot). The triBlot fractionates nuclei from the cytoplasm, allowing analysis of cytoplasmic proteoforms. Then, nuclei-laden regions are laser excised to perform PCR/RT-qPCR. We correlate DNA, mRNA, and protein expression from the same few cells, a step forward in 'omics that measure diverse protein forms. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

关键词： Proteins

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Protein dot array patterning for study of cell adhesion and migration

Protein dot array patterning for study of cell adhesion and ...

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International Nano-Optoelectronic Workshop, iNOW

作者： Junyu, Mai Song, Li Xiang, Zhang NSF Nanoscale Science and Engineering Center UCSF/UC Berkeley Joint Graduate Group in Bioengineering Berkeley CA 94720 United States Department of Bioengineering UC Berkeley Berkeley CA 94720 United States NSF Nanoscale Science and Engineering Center UC Berkeley Berkeley CA 94720 United States

Fibronectin is patterned on silicone rubber surfaces in arrays of 2pm diameter dots using contact removal method. Cells are cultured on the patterned surface and their migration and the structure of actin cytoskeleton... 详细信息

ISBN: (纸本)1424415918

关键词： Proteins

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Probing cytoskeleton dynamics with multi-directional topographical guidance 11

Probing cytoskeleton dynamics with multi-directional topogra...

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11th International Conference on Miniaturized Systems for Chemistry and Life Sciences, uTAS 2007

作者： Mai, Junyu Sun, Cheng Li, Song Zhang, Xiang NSF Nanoscale Science and Engineering Center United States UCSF UC Berkeley Joint Graduate Group in Bioengineering University of California Berkeley United States

We used microfabricated topographical guidance to manipulate the organization of a single cell cytoskeleton, and to probe a cell from multiple directions to study the molecular mechanisms regulating the coordination p... 详细信息

ISBN: (纸本)9780979806407

关键词： Surface topography

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Technical artifacts in droplet-microfluidics-based single-nuclei RNA-sequencing 25

Technical artifacts in droplet-microfluidics-based single-nu...

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25th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2021

作者： Gupta, Anushka Streets, Aaron UC Berkeley-UC San Francisco Graduate Program in Bioengineering BerkeleyCA United States Chan Zuckerberg Biohub San FranciscoCA United States

ISBN: (纸本)9781733419031

Droplet microfluidic platforms have recently become a powerful tool for sequencing the transcriptomes of single nuclei in a ultrahigh-throughput format. However, single-nuclei RNA-sequencing (snRNA-seq) introduces severe sources of technical noise and transcript detection bias which distorts true biological heterogeneity. In this work, we performed a comparative study between droplet-based single-cell RNA-sequencing (scRNA-seq) and snRNA-seq on human preadipocytes to identify and characterize primary sources of technical artifacts associated with snRNA-seq, and propose strategies to account for these artifacts. © 2021 MicroTAS 2021 - 25th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

关键词： RNA

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Miniscope3D:optimized single-shot miniature 3D fluorescence microscopy

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Light(Science & Applications) 2020年第1期9卷 367-379页

作者： Kyrollos Yanny Nick Antipa William Liberti Sam Dehaeck Kristina Monakhova Fanglin Linda Liu Konlin Shen Ren Ng Laura Waller UCB/UCSF Joint Graduate Program in Bioengineering University of CaliforniaBerkeleyCA 94720USA Department of Electrical Engineering&Computer Sciences University of CaliforniaBerkeleyCA 94720USA TIPs Department Universite libre de Bruxelles(ULB)1050 BrusselsBelgium

Miniature fluorescence microscopes are a standard tool in systems ***,widefield miniature microscopes capture only 2D information,and modifications that enable 3D capabilities increase the size and weight and have poor resolution outside a narrow depth ***,we achieve the 3D capability by replacing the tube lens of a conventional 2D Miniscope with an optimized multifocal phase mask at the objective’s aperture *** the phase mask at the aperture stop significantly reduces the size of the device,and varying the focal lengths enables a uniform resolution across a wide depth *** phase mask encodes the 3D fluorescence intensity into a single 2D measurement,and the 3D volume is recovered by solving a sparsity-constrained inverse *** provide methods for designing and fabricating the phase mask and an efficient forward model that accounts for the fieldvarying aberrations in miniature *** demonstrate a prototype that is 17mm tall and weighs 2.5 grams,achieving 2.76μm lateral,and 15μm axial resolution across most of the 900×700×390μm^(3) volume at 40 volumes per *** performance is validated experimentally on resolution targets,dynamic biological samples,and mouse brain *** with existing miniature single-shot volume-capture implementations,our system is smaller and lighter and achieves a more than 2×better lateral and axial resolution throughout a 10×larger usable depth *** microscope design provides single-shot 3D imaging for applications where a compact platform matters,such as volumetric neural imaging in freely moving animals and 3D motion studies of dynamic samples in incubators and lab-on-a-chip devices.

关键词： enable resolution narrow

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On-chip western blotting: In-situ renaturation of SDS-protein complexes unifies sodium dodecyl sulfate (SDS) sizing & blotting in one microdevice

On-chip western blotting: In-situ renaturation of SDS-protei...

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15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011

作者： Hou, Chenlu Herr, Amy E. University of California Electrical Engineering and Computer Science Berkeley United States University of California Bioengineering Berkeley United States University of California UCSF Graduate Program in Bioengineering Berkeley United States

ISBN: (纸本)9781618395955

Western blotting readily identifies specific proteins amidst complex biological backgrounds [1, 2]. Nevertheless, immunob-lotting suffers from tremendous labor-intensive and time-intensive requirements [3]. The slab-gel assays require 1-2 days for completion with multiple hands-on "blotting" steps and yield semi-quantitative information. Recently, our group has introduced new approaches for completing Western blotting. The microfluidic integration strategies introduced and used allow rapid results reporting, full assay automation, and limited sample consumption (1-10 uL). Our integration strategies use spatial, temporal, and spatiotemporal modulation of separation mechanisms in fully electrophoretic systems. The present study reports on recapitulation of immunoaffinity in previously sized proteins, using novel in-transit electrophoretic removal of SDS from SDS-protein complexes. Early results show both the length- and timescales for protein 'renaturation' are compatible with on-chip operation. Further, substantial binding affinity is recapitulated using this streamlined and promising approach.

关键词： Sodium dodecyl sulfate

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Rapid fabrication method for plastic microfluidic devices with embedded microelectrodes and its application to electroporation and cell lysis on chip 18

Rapid fabrication method for plastic microfluidic devices wi...

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18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

作者： Paredes, Jacobo Chooljian, Marc Fink, Kathryn D. Liepmann, Dorian CEIT and Tecnun University of Navarra Spain CIC MicroGUNE Spain UC Berkeley San Francisco Graduate Program in Bioengineering United States Department of Bioengineering UC Berkeley United States Berkeley Sensors and Actuators Center United States

ISBN: (纸本)9780979806476

Methods of fabricating microfluidic devices that are scalable, simple, and inexpensive are necessary to ease the transition of microfluidics from an academic to an industrial discipline. Here we present a method for rapid prototyping of microfluidic devices with embedded electrodes in plastic based on electroplating and hot embossing techniques. We then discuss its applicability to electroporation and lysis, two of many potential applications that could benefit from this manufacturing method. © 14CBMS.

关键词： Fabrication

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Glucocorticoids cause mandibular bone fragility and suppress osteocyte perilacunar-canalicular remodeling

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Bone Reports 2018年 9卷 145-153页

作者： Alemi, A. Sean Mazur, Courtney M. Fowler, Tristan W. Woo, Jonathon J. Knott, P. Daniel Alliston, Tamara Department of Otorhinolaryngology Head and Neck Surgery University of California San Francisco United States Department of Orthopaedic Surgery University of California San Francisco United States UC Berkeley-UCSF Graduate Program in Bioengineering United States

Osteocytes support dynamic, cell-intrinsic resorption and deposition of bone matrix through a process called perilacunar/canalicular remodeling (PLR). In long bones, PLR depends on MMP13 and is tightly regulated by PTH, sclerostin, TGFβ, and glucocorticoids. However, PLR is regulated differently in the cochlea, suggesting a mechanism that is anatomically distinct. Unlike long bones, the mandible derives from neural crest and exhibits unique susceptibility to medication and radiation induced osteonecrosis. Therefore, we sought to determine if PLR in the mandible is suppressed by glucocorticoids, as it is in long bone. Hemimandibles were collected from mice subcutaneously implanted with prednisolone or vehicle containing pellets for 7, 21, or 55 days (n = 8/group) for radiographic and histological analyses. Within 21 days, micro-computed tomography revealed a glucocorticoid-dependent reduction in bone volume/total volume and trabecular thickness and a significant decrease in bone mineral density after 55 days. Within 7 days, glucocorticoids strongly and persistently repressed osteocytic expression of the key PLR enzyme MMP13 in both trabecular and cortical bone of the mandible. Cathepsin K expression was significantly reduced only after 55 days of glucocorticoid treatment, at which point histological analysis revealed a glucocorticoid-dependent reduction in the lacunocanalicular surface area. In addition to reducing bone mass and suppressing PLR, glucocorticoids also reduced the stiffness of mandibular bone in flexural tests. Thus, osteocyte PLR in the neural crest-derived mandible is susceptible to glucocorticoids, just as it is in the mesodermally-derived femur, highlighting the need to further study PLR as a target of drugs, and radiation in mandibular osteonecrosis. © 2018

关键词： Glucocorticoids Mandible Osteocyte Perilacunar/canalicular remodeling

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Motion artifacts on 240Hz OLED stereoscopic 3D displays

Motion artifacts on 240Hz OLED stereoscopic 3D displays

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2014 SID International Symposium

作者： Johnson, Paul Kim, Joohwan Hoffman, David M. Vargas, Andres Banks, Martin S. UC Berkeley - UCSF Graduate Program in Bioengineering United States University of California Berkeley Vision Science Program 360 Minor Hall Berkeley CA United States Samsung Display Americas Lab San Jose CA United States

We examined the visibility of motion artifacts-judder, motion blur, and edge banding-on a Samsung 240Hz stereoscopic 3D (S3D) OLED display. We determined the relative contributions of the frame rate of the content, update rate of the panel, duty cycle, and flash number. We compared the visibility of artifacts on the Samsung display with those on a 60Hz S3D LCD. Short duty cycles and low flash numbers reduce the visibility of motion artifacts. © 2014 SID.

关键词： Visibility

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