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Transcriptome analysis and functional gene identification reveals potential mechanisms of heat stress response of Lactiplantibacillus plantarum CGMCC8198

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Food Bioscience 2023年第1期51卷 152-158页

作者： Jia-Yi Da Mao-Sheng Xi Han-Lu Li Min-Min Liu Cheng-Hui Zhou Zhong-Yuan Li Ya-Jian Song Sa Zhou Tong-Cun Zhang Xue-Gang Luo Key Laboratory of Industrial Fermentation Microbiology of the Ministry of Education and Tianjin Key Laboratory of Industrial Microbiology College of BiotechnologyTianjin University of Science and TechnologyTianjin300457China Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control Tianjin300457China

Lactiplantibacillus plantarum is an important probiotic bacterium and extensively applied to functional *** survival is strongly affected by the heat-shock process during *** is thought to be able to enhance bacteria resistance to heat *** this study,the results of transcriptomics and bioinformatics analysis showed that alcohol dehydrogenase adhE-like,phosphoribose-ATP pyrophosphatase hisE,flavodoxin ykuN,and dihydropterin aldolase folB might be related with heat resistance of *** ***,after these genes were expressed in Escherichia coli,survival rate,hydrophobicity and membrane integrity of the recombinant bacteria under high temperature were also significantly ***,by screening the strains after multiple rounds of acclimation,a strain(XH)with better heat tolerance was obtained,and the results of qRT-PCR showed that these four genes,especially hisE,were upregulated in the acclimated strain when it was treated with high *** findings indicated that adhE-like and some other potential genes could improve the heat resistance of lactic acid bacteria,and might be used as indicators for screening of heat-tolerant probiotics strains.

关键词： Probiotic Heat stress Functional genes Acclimation

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Efficient production of 14α‑OH‑AD by engineered Mycolicibacterium neoaurum via coupled cofactor and reconstructed electron transport system

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Systems Microbiology and Biomanufacturing 2023年第2期3卷 358-369页

作者： Chenwei Zhang Yanbing Shen Yuanyuan Gao Zehui Zan Min Wang Key Laboratory of Industrial Fermentation Microbiology Ministry of EducationTianjin Key Laboratory of Industrial MicrobiologyTianjin University of Science and TechnologyTianjinChina Engineering Research Center of Microbial Metabolism and Fermentation Process Control College of BiotechnologyTianjin University of Science and TechnologyTianjin 300457People’s Republic of China

14α-hydroxy-androst-4-ene-3,17-dione(14α-OH-AD)is an important precursor for the synthesis of steroid drugs with anticancer and carcinolytic ***,14α-OH-AD was mostly synthesized by whole-cell fermentation of mold fungi using androstenedione(AD)as a substrate,which had difficulties in product isolation and purification as well as problems of high production *** this study,the source of the 14α-hydroxylase gene was *** 14α-hydroxylase genes were heterologously expressed in Mycolicibacterium neoaurum(MNR)M3ΔksdD,which enabled the one-step biotransformation from the cheap substrate phytosterols(PS)to 14α-OH-AD,reducing the difficulty of product purification and production *** is more,to alleviate the problem of poor activity of 14α-hydroxylase,the 14α-hydroxylase gene was co-expressed with the electron transport chain element genes and the coenzyme regeneration genes,and a superior engineered strain MNR M3ΔksdD/pMV261-14α-G6PDH was ***,the transformation conditions were optimized for the transformation of PS by the engineered *** molar yield of 14α-OH-AD reached to 60.4±2.3%(about 0.22 g/L productivity).This study investigated for the first time the effects of the tandem electron transport chain element genes and the tandem coenzyme regeneration genes on the 14α-hydroxylation reaction,providing a theoretical basis for the industrial production of 14α-OH-AD.

关键词： 14α-hydroxy-androst-4-ene-3,17-dione Mycolicibacterium neoaurum 14α-hydroxylase Electron transportation Coenzyme regeneration

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De novo transcriptome assembly of Aureobasidium melanogenum CGMCC18996 to analyze theβ-poly(L-malic acid)biosynthesis pathway under the CaCO_(3) addition

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Food Science and Human Wellness 2023年第4期12卷 1248-1256页

作者： Genan Wang Haisong Yin Tingbin Zhao Donglin Yang Shiru Jia Changsheng Qiao Key Laboratory of Industrial Fermentation Microbiology(Tianjin University of Science and Technology) Ministry of EducationTianjin 300457China Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control College of BiotechnologyTianjin University of Science and TechnologyTianjin 300457China School of Bioengineering Tianjin Modern Vocational Technology CollegeTianjin 300350China Tianjin Huizhi Biotrans Bioengineering Co. Ltd.Tianjin 300457China

β-Poly(L-malic acid)(PMLA)is a water-soluble biopolymer used in food,medicine and other *** date,the biosynthesis pathway of PMLA has not been fully *** this study,we sequenced the transcriptom e of strain Aureobasidium melanogenum under 20 g/L CaCO_(3) *** resulting sequencing reads were assembled and annotated for the differentially expressed genes(DEGs)analysis and novel transcripts *** result indicated that with the CaCO_(3) addition,the tricarboxylic cycle(TCA)cycle and glyoxylate pathway were up-regulated,and it also found that a non-ribosomal peptide synthetase(NRPS)like protein was highly *** DEGs analysis showed a high expression level of malate dehydrogenase(MDHC)and phosphoenolpyruvate carboxykinase(PCKA)in the CaCO_(3) group,which indicated a cytosolic malate *** speculated that the malate should be transported to or synthesized in the cytoplasm,which was then polymerized to PMLA by the NRPS-like protein,accompanied by the up-regulated TCA cycle providing ATP for the *** on the analysis,we assumed that an NRPS-like protein,the TCA cycle,and the cytosolic malate together are contributing to the PMLA biosynthesis.

关键词： De novo transcriptome analysis β-Poly(L-malic acid) Aureobasidium melanogenum

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Monascus vinegar alleviates high-fat-diet-induced inflammation in rats by regulating the NF-κB and PI3K/AKT/mTOR pathways

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Food Science and Human Wellness 2022年第4期11卷 943-953页

作者： Huanmei Meng Jia Song Bingqian Fan Yingqi Li Jiaojiao Zhang Jinping Yu Yu Zheng Min Wang State Key Laboratory of Food Nutrition and Safety Key Laboratory of Industrial Fermentation MicrobiologyTianjin Engineering Research Centre of Microbial Metabolism and Fermentation Process ControlCollege of BiotechnologyTianjin University of Science&TechnologyTianjin 300457China Shandong Engineering Research Centre of Condiment Fermentation Technology Shandong Yutu Co.LtdZibo 255300China

Monascus vinegar(MV) is a typical fermented food with various health-promoting effects. This study aimed to evaluate the role of MV in alleviating high-fat-diet-induced inflammation in rats with hyperlipidemia and elucidate the possible regulatory mechanisms. In the study, serum lipid profiles, liver pathology and liver inflammatory cytokines were analyzed in hyperlipidemia rats with MV(0.5 mL/kg mb, 2 mL/kg mb). Results showed that the administration of MV alleviated dyslipidemia by decreasing the serum and liver levels of triglyceride and total cholesterol. Increase in hepatic lipase and carnitine palmitoyl transferase 1(CPT-1)levels and decrease in hepatocyte steatosis, nephritis, and intestinal tissue injury in the HD group showed that high-dose MV can significantly suppress hepatic lipid accumulation and steatosis. In addition, compared with the model(MOD) group, the HD group showed significantly down-regulated the level of serum or hepatic alanine aminotransferase(ALT), aspartate aminotransferase(AST), CPT-1, interleukin(IL)-2, IL-6, IL-12,and tumor necrosis factor α(TNF-α). Moreover, the HD group showed repressed hepatic nuclear factor κB(NF-κB) pathway and inactivated phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)/mammalian target of rapamycin(mTOR) pathway mitigated liver inflammation. Similar results were obtained from cell experiments. Collectively, these findings revealed that MV might attenuate high-fat-diet-induced inflammation by inhibiting the NF-κB and PI3K/Akt/mTOR pathways.

关键词： Monascus vinegar Hyperlipidemia Inflammation NF-κB PI3K/AKT/mTOR

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Carboxymethylation of the polysaccharide from the fermentation broth of Marasmius androsaceus and its antidepressant mechanisms

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Food Science and Human Wellness 2023年第6期12卷 2417-2427页

作者： Yu Qiu Yan Su Jia Song Fangming Mou Jia Gou Xiaoqi Geng Xinyue Li Zhiqiang Nie Jianxin Wang Yu Zheng Min Wang State Key Laboratory of Food Nutrition and Safety Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process ControlCollege of BiotechnologyTianjin University of Science&TechnologyTianjin 300457China Key Laboratory of Chemical Biology and Molecular Engineering Ministry of EducationInstitute of BiotechnologyShanxi UniversityTaiyuan 030006China Wisconsin Center for NanoBioSystems School of PharmacyUniversity of Wisconsin-MadisonMadisonWI 53706USA Pharmaceutical Sciences Division School of PharmacyUniversity of Wisconsin-MadisonMadisonWI 53706USA

To investigate the structure-activity relationship of polysaccharide and obtain a better antidepressant polysaccharide,the antidepressant-like activity of a carboxymethyl polysaccharide(C-MEPS2)subjected to submerged fermentation was systematically ***12-H cell and Kunming mice were used to investigate the differences and their mechanism in the antidepressant effects of C-MEPS2 and *** experiments have showed that C-MEPS2 has a better antidepressant effect than MEPS2.C-MEPS2 could exert antidepressant effects related to catecholamine synthesis with specifi c sites of TH,D2DR,and *** addition,C-MEPS2 could repair the Res-induced damage in PC12-H cell,stabilize the mitochondrial membrane potential and regulate intracellular Ca^(2+) concentration,thus reducing cell apoptosis caused by *** common dosing experiments on animals further proved that CMEPS2 could signifi cantly improve the antidepressant effect of derivatives without affecting the antidepressant mechanism of *** is speculated that it may be related to carboxymethylated modifi cation.

关键词： Carboxymethyl polysaccharide Anti-depressant Catecholamine

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Development of novel dead-end filtration strategy for isolation of pullulan broth

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process Biochemistry 2025年 156卷 359-368页

作者： Linyu Lu Yiwen Li Qingli Guan Tingbin Zhao Weifeng Cao Changsheng Qiao College of Bioengineering Tianjin University of Science and Technology Tianjin 300457 China Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control College of Biotechnology Tianjin University of Science and Technology Tianjin 300457 China Tianjin Beiyang Baichuan Biotechnology Co. LTD Tianjin 300457 China Tianjin Huizhi Biotrans Bioengineering Co. Ltd Tianjin 300457 China

Pullulan, a natural extracellular polysaccharide, is widely used in food and related industries, cosmetics, and pharmaceuticals. The high viscosity of its fermentation broth poses significant challenges for efficient separation, making the process both difficult and time-consuming. In this study, cross-flow membrane filtration was first applied to separate a two-fold diluted broth (feed solution), where membranes with pore sizes ranging from 150 kDa to 0.8 μm rejected over 99 % of cells and approximately 50 % of pullulan. This phenomenon was attributed to higher shear forces increasing pullulan particle size in solution, thereby reducing its recovery rate. To address this limitation, a dead-end filtration strategy was developed using a 1–3 μm filtration medium with 1 % filter aid. During concentration-mode filtration, fouling mechanisms in the first stage showed indistinct differences between standard and intermediate blocking, while the second stage exhibited unclear distinctions between complete and intermediate blocking. A favorable membrane regeneration strategy was identified: sequential washing with fresh water followed by detergent powder. This method achieved 99.54 % biomass removal and a pullulan recovery rate of 90.67 %. Overall, the dead-end filtration strategy presents a novel approach for separating high-viscosity biopolymers, offering both efficiency and practicality for industrial applications.

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Deacetoxycephalosporin C synthase(expandase):research progress and application potential

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Synthetic and Systems Biotechnology 2021年第4期6卷 396-401页

作者： Xiaofan Niu Jian Zhang Xianli Xue Depei Wang Lin Wang Qiang Gao Key Laboratory of Industrial Fermentation Microbiology Ministry of EducationCollege of BiotechnologyTianjin University of Science and TechnologyTianjin300457China National Demonstration Center for Experimental Bioengineering Education(Tianjin University of Science and Technology) Tianjin300457China Tianjin Microbial Metabolism and Fermentation Process Control Technology Engineering Center Tianjin300457China College of Artificial Intelligence Tianjin University of Science and TechnologyTianjin300457China

Cephalosporins play an indispensable role against bacterial *** C synthase(DAOCS),also called expandase,is a key enzyme in cephalosporin biosynthesis that epoxides penicillin to form the hexavalent thiazide ring of *** in fungus Acremonium chrysogenum was identified as a bifunctional enzyme with both ring expansion and hydroxylation,whereas two separate enzymes in bacteria catalyze these two *** this review,we briefly summarize its source and function,improvement of the conversion rate of penicillin to deacetyloxycephalosporin C through enzyme modification,crystallography features,the prediction of the active site,and application perspective.

关键词： Deacetyloxycephalosporin C synthase Expandase Streptomyces clavuligerus Acremonium chrysogenum Progress Application

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Characterization of Melanoidins from Shanxi Aged Vinegar and Their Behaviors of in Vitro Simulated Digestion and Colonic fermentation

SSRN

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SSRN 2024年

作者： Wang, Jun Zhang, Nannan Xia, Ting Nie, Yaning Zhang, Xiaodong Lang, Fanfan Liang, Kai Li, Tianmeng Wang, Min State Key Laboratory of Food Nutrition and Safety Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control College of Biotechnology Tianjin University of Science and Technology Tianjin300457 China Shanxi Provincial Key Laboratory for Vinegar Fermentation Science and Engineering Shanxi Zilin Vinegar Industy Co.Ltd. Taiyuan030400 China

Melanoidins can be produced during the Cupei process of Shanxi aged vinegar. The aim of this study is to elucidate the structure, physicochemical properties, and potential behavior of vinegar melanoidins (VM) during digestion and microbial fermentation in vitro. Our findings revealed that the primary constituent of VM were carbohydrates, and showed a non-crystalline structure with the particle size of 166.46±10.06 nm. VM exhibited stability in vitro digestion process. However, the carbohydrate contents and Mw were reduced. TPC (29.33 ± 8.226-79.48 ± 9.522 mg GAE/g DW), TFC (0.076 ± 0.007-0.422 ± 0.024 mg RE/g DW), antioxidant activity and SCFAs were significantly increased during the colonic fermentation process. In addition, VM exhibited intestinal modulatory effects on the microbiota by promoting an increase in beneficial bacteria including Firmicutes and Bifidobacterium. Collectively, these results suggest that VM as a potential prebiotic can be applied in the field of functional food. © 2024, The Authors. All rights reserved.

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Antibacterial mechanism and computer simulation analysis of a novel antimicrobial peptide inducing membrane disintegration in Gram-positive bacteria

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Food Bioscience 2025年 71卷

作者： Meili Cui Haoyan Sun Xia Liu Zirong Huang Zhenhua Su Yu Zheng Yanbing Shen Min Wang State Key Laboratory of Food Nutrition and Safety Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control College of Biotechnology Tianjin University of Science and Technology Tianjin 300457 China

LAB-4, a novel antimicrobial peptide screened from the microbiome of Shanxi aged vinegar, has been shown to inhibit Gram-positive bacterial infections, but its antibacterial mechanism remains unclear. This study aimed to elucidate the antibacterial mechanism of LAB-4 against Gram-positive bacteria at the cellular and molecular levels. A series of in vitro assays (including antibacterial activity, safety, stability, membrane integrity, and membrane permeability analyses) and computational simulation techniques (molecular docking and molecular dynamics simulations) were employed to systematically investigate the antibacterial efficacy and mechanism of LAB-4. The results showed that LAB-4 exhibited potent antimicrobial activity, rapid bactericidal effects, minimal hemolytic activity, no cytotoxicity, and high stability under various adverse conditions. In vitro assays revealed that LAB-4 inhibited bacterial growth in a concentration-dependent manner, primarily causing irreversible membrane damage in Gram-positive bacteria by disrupting membrane integrity, increasing membrane permeability, and inducing intracellular content leakage. Computational simulations indicated that LAB-4 initially bound to peptidoglycan, after which its positively charged amino acid residues (primarily Arg, His, and Lys) were drawn to negatively charged membrane phospholipids through electrostatic interactions and extensive hydrogen bonding. LAB-4 subsequently inserted into the phosphatidyl-phosphate headgroups of the POPG and POPE bilayers via hydrophobic interactions, causing an increase in the area per lipid and a decrease in the membrane thickness. These findings demonstrated that LAB-4 exerted its antibacterial effect by inducing membrane disruption and underscored its potential as a safe and effective natural antimicrobial agent for applications in the food industry.

关键词： Antibacterial mechanism Computer simulations Membrane-active peptide Phospholipid bilayer Vitro experiments

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Structural and Physicochemical Properties and Changes in Vitro Digestion and fermentation of Dietary Fiber from Tea Residues Modified by fermentation

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SSRN 2024年

作者： Xia, Ting Nie, Yaning Chen, Yang Zhang, Nannan Wang, Yongqi Liu, Shunhang Bai, Xiaoli Cao, Hailong Xu, Yongquan Wang, Min State Key Laboratory of Food Nutrition and Safety Tianjin Engineering Research Center of Microbial Metabolism and Fermentation Process Control College of Biotechnology Tianjin University of Science and Technology Tianjin300457 China Yunnan Tasly Deepure Bio-Tea Group Co Ltd Yunnan665099 China State Key Laboratory of Core Technology in Innovative Chinese Medicine Tasly Holding Group Co. Ltd Tianjin300410 China Department of Gastroenterology and Hepatology General Hospital Tianjin Medical University National Key Clinical Specialty Tianjin Institute of Digestive Diseases Tianjin Key Laboratory of Digestive Diseases Tianjin300052 China

The aim of this study was to investigate the structure, physicochemical properties, and changes in vitro digestion and fermentation between unfermented tea residue dietary fiber (UDF) and fermented tea residue soluble dietary fiber (FSDF). The results showed that the total dietary fiber yield in FSDF was significantly increased from 61.87% to 75.37% after fermentation modification. Monosaccharide composition analysis showed that xylose and glucose accounted for a higher proportion in FSDF. FSDF had smaller particle size, lower crystallinity and higher thermal stability. The water holding capacity, oil holding capacity and water swelling capacity of FSDF were significantly increased. Rheological properties showed that FSDF exhibited higher viscosity and better elastic than UDF. Furthermore, FSDF generated more short-chain fatty acids, and the structure was looser than UDF, which was easier to be utilized by intestinal flora. These findings provided higher value of dietary fiber in tea residue by fermentation modification as functional products. © 2024, The Authors. All rights reserved.

关键词： Tea

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