Objective We aimto determine the utility of quantifying DXA-derived surrogates of CT measures including thigh cross-sectional muscle area(CSA) and muscle attenuation. Methods We used a retrospective case-cohort study ...
详细信息
Objective We aimto determine the utility of quantifying DXA-derived surrogates of CT measures including thigh cross-sectional muscle area(CSA) and muscle attenuation. Methods We used a retrospective case-cohort study design using data from the HealthAging and Body Composition(HealthABC) *** were 111 participants that had a hip fracture during the 8.7-year study period.A cohortof 212 non-fracture participants wasrandomly selected
＜正＞Objective:To examine theindependent association ofbody shape（total volume,trunk volume,and trunk to leg volume ratio）tofracture risk. Methods:We performed a cross-sectional study of the NHANES 1999-2004 survey...
＜正＞Objective:To examine theindependent association ofbody shape（total volume,trunk volume,and trunk to leg volume ratio）tofracture risk. Methods:We performed a cross-sectional study of the NHANES 1999-2004 survey data downloaded from the study *** individualshad both a valid dual-energy Xray absorptiometry（DXA） scan and known fracture status. The body shape measureswere derived using DXA whole
We introduce a 'hands free' proteomic tool with the potential to radically transform the status quo of cancer protein bi-omarker validation. Here, we focus on a challenging and broadly relevant target in this ...
详细信息
ISBN:
(纸本)9781618395955
We introduce a 'hands free' proteomic tool with the potential to radically transform the status quo of cancer protein bi-omarker validation. Here, we focus on a challenging and broadly relevant target in this analyte class - the prostate cancer marker prostate-specific antigen (PSA). We introduce light-activatable, volume accessible polyacrylamide separation gels (LAVAgels) that capture electrophoretically separated PSA isoforms within a highly multiplexed (16-channel) microfluidic assay format. The 3D LAVAgel matrix offers unprecedented target capture duty, with capture efficiencies greater than 100fold higher than for existing surface chemistries. Our optofluidic separations strategy harnesses tremendous micro/nanoscale transport advantages to yield unmatched speed and efficiency - major barriers to realizing high-throughput proteomics.
The disassembly of a core-satellite nanostructured substrate is presented as a colorimetric biosensor observable under dark-field illumination. The fabrication method described herein utilizes thiol-mediated adsorptio...
详细信息
Western blotting readily identifies specific proteins amidst complex biological backgrounds [1, 2]. Nevertheless, immunob-lotting suffers from tremendous labor-intensive and time-intensive requirements [3]. The slab-g...
详细信息
ISBN:
(纸本)9781618395955
Western blotting readily identifies specific proteins amidst complex biological backgrounds [1, 2]. Nevertheless, immunob-lotting suffers from tremendous labor-intensive and time-intensive requirements [3]. The slab-gel assays require 1-2 days for completion with multiple hands-on "blotting" steps and yield semi-quantitative information. Recently, our group has introduced new approaches for completing Western blotting. The microfluidic integration strategies introduced and used allow rapid results reporting, full assay automation, and limited sample consumption (1-10 uL). Our integration strategies use spatial, temporal, and spatiotemporal modulation of separation mechanisms in fully electrophoretic systems. The present study reports on recapitulation of immunoaffinity in previously sized proteins, using novel in-transit electrophoretic removal of SDS from SDS-protein complexes. Early results show both the length- and timescales for protein 'renaturation' are compatible with on-chip operation. Further, substantial binding affinity is recapitulated using this streamlined and promising approach.
Here we present a fully integrated lab-on-a-chip (LOC) system that incorporates cell preconcentration, polymerase chain reaction (PCR), and capillary electrophoretic (CE) microchannel for rapid and highly sensitive pa...
详细信息
暂无评论